Isolation and use of fetal urogenital sinus expressed sequences

ABSTRACT

The invention comprises methods for identifying biomarkers useful for prognostic or diagnostic assays of human prostate disease, and for identifying those fetal genes which are differentially expressed between prostate cancers versus normal or benign prostate.

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit under 35 U.S.C. section 119(e) of co-pending U.S. provisional application Ser. No. 60/085,383, filed May 14, 1998, the entire text of which is herein incorporated by reference without disclaimer.

STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY-SPONSORED RESEARCH AND DEVELOPMENT

[0002] Part of the work performed during development of this invention utilized U.S. Government funds. The U.S. Government has certain rights in this invention. This work was supported by National Institutes of Health Grant PHFDK47596.

I. FIELD OF THE INVENTION

[0003] The present invention relates to the study of normal and diseased prostate development. More particularly, the present invention relates to methods and compositions relates to novel nucleotide sequences which can be used for the diagnosis, prognosis and treatment of prostatitis and benign and malignant growth of the prostate gland. More particularly, the present invention concerns probes and methods useful in diagnosing, identifying and monitoring the progression of diseases of the prostate through measurements of fetal gene products.

II. BACKGROUND OF THE INVENTION

[0004] Prostatic Hyperplasia

[0005] Development of prostatic hyperplasia is an almost universal phenomenon in aging men. The prostate weighs only a few grams at birth; at puberty it undergoes androgen-mediated growth and reaches the adult size of about 20 g by age 20. It remains stable in size for about 25 years, and during the fifth decade a second growth spurt commences in the majority of men. Consequently, the disorder affects men over the age of 45 and increases in frequency with age so that by the eighth decade more than 90 percent of men have prostatic hyperplasia at autopsy. Since the development of BPH is not a major cause of death, the development of effective therapies has been slow despite BPH been a leading cause of morbidity in elderly men. The prostate surrounds the urethra, and prostatic hyperplasia is the most common cause of obstruction to urinary outflow in men. The disorder occurs in all populations but may be less common in the Orient. The mean age for development of symptomatic disease is about 65 years for whites and about 60 years for blacks. At present, it is not clear whether prostatic hyperplasia predisposes the prostate to the development of prostatic cancer (Harrison's Principles of Internal Medicine, Chapter 97, p 596, 14th Edition, McGraw Hill, 1999).

[0006] Unlike the pubertal growth spurt which involves the gland diffusely, prostatic hyperplasia begins in the periurethral region as a localized proliferation and progresses to compress the remaining normal gland. Histologically, the hyperplastic tissue is nodular and composed of varying amounts of glandular epithelium, stroma, and smooth muscle. The hyperplasia can compress and obstruct the urethra; the hyperplastic gland can also grow posteriorly to obstruct the rectum and cause constipation.

[0007] At present, the pathogenesis is not well understood, but two necessary features for the process are aging and the presence of testes; whether the testes play a direct or permissive role is not known, but the active androgen that mediates prostatic growth at all ages is dihydrotestosterone, which is formed within the prostate from plasma testosterone (Harrison's Principles of Internal Medicine, Chapter 97, pp 597, 14th Edition, McGraw Hill, 1999).

[0008] Prostatic Carcinoma

[0009] Cancer of the prostate is the most common malignancy in men in the United States and the third most common cause of cancer death in men above age 55 (after carcinomas of the lung and colon). In the United States there are approximately 317,000 newly diagnosed cases and more than 41,000 deaths from the disorder each year. Only about a third of cases identified at autopsy are manifest clinically. The disease is rare before age 50, and the incidence increases with age. The frequency varies in different parts of the world. The United States has 14 deaths per 100,000 mean per year, compared with 22 for Sweden and 2 for Japan. However, Japanese immigrants to the United States develop prostatic cancer at a frequency similar to other men in this country, suggesting that environmental factors are the principal cause for population differences. The disease is more common among American blacks than whites; the reason for this difference is not known. Some carcinomas of the prostate are slow-growing and may persist for long periods without causing significant symptoms, whereas others behave aggressively. It is not known whether tumors can become more malignant with time (Harrison's Principles of Internal Medicine, Chapter 97, p 598, 14th Edition, McGraw Hill, 1999).

[0010] Prostatitis

[0011] The term prostatitis has been used for various inflammatory conditions affecting the prostate, including acute and chronic infections with specific bacteria and, more commonly, instances in which signs and symptoms of prostatic inflammation are present but no specific organisms can be detected. Patients with acute bacterial prostatitis can usually be identified on the basis of typical symptoms and signs, pyuria, and bacteriuria. To classify a patient with suspected chronic prostatitis correctly, first-void and midstream urine specimens, a prostatic expressate, and a postmassage urine specimen should be quantitatively cultured and evaluated for numbers of leukocytes. On the basis of the results of these studies, patients can be classified as having chronic bacterial prostatitis, chronic nonbacterial prostatitis, or prostatodynia. Patients with suspected chronic prostatitis usually have low back pain, perineal or testicular discomfort, mild dysuria, and lower urinary obstructive symptoms. Microscopic pyuria may be the only objective manifestation of prostatic disease (Harrison's Principles of Internal Medicine, Chapter 131, pp823, 14th Edition, McGraw Hill, 1999).

[0012] Carcinoma of the prostate (PCA) is the second-most frequent cause of cancer related death in men in the United States (Boring, 1993). The increased incidence of prostate cancer during the last decade has established prostate cancer as the most prevalent of all cancers (Carter and Coffey, 1990). Although prostate cancer is the most common cancer found in United States men, (approximately 200,000 newly diagnosed cases/year), the molecular changes underlying its genesis and progression remain poorly understood (Boring et al., 1993). According to American Cancer Society estimates, the number of deaths from PCA is increasing in excess of 8% annually.

[0013] An unusual challenge presented by prostate cancer is that most prostate tumors do not represent life threatening conditions. Evidence from autopsies indicate that 11 million American men have prostate cancer (Dbom, 1983). These figures are consistent with prostate carcinoma having a protracted natural history in which relatively few tumors progress to clinical significance during the lifetime of the patient. If the cancer is well-differentiated, organ-confined and focal when detected, treatment does not extend the life expectancy of older patients.

[0014] Unfortunately, the relatively few prostate carcinomas that are progressive in nature are likely to have already metastasized by the time of clinical detection. Survival rates for individuals with metastatic prostate cancer are quite low. Between these two extremes are patients with prostate tumors that will metastasize but have not yet done so. For these patients, surgical removal of their prostates is curative and extends their life expectancy. Therefore, determination of which group a newly diagnosed patient falls within is critical in determining optimal treatment and patient survival.

[0015] Although clinical and pathologic stage and histological grading systems (e.g., Gleason's) have been used to indicate prognosis for groups of patients based on the degree of tumor differentiation or the type of glandular pattern (Carter and Coffey, 1989; Diamond et al., 1982), these systems do not predict the progression rate of the cancer. While the use of computer-system image analysis of histologic sections of primary lesions for “nuclear roundness” has been suggested as an aide in the management of individual patients (Diamond et al., 1982), this method is of limited use in studying the progression of the disease.

[0016] It is known that the processes of transformation and tumor progression are associated with changes in the levels of messenger RNA species (Slamon et al., 1984; Sager et al., 1993; Mok et al., 1994; Watson et al., 1994). Recently, a variation on PCR analysis known as RNA fingerprinting has been used to identify messages differentially expressed in ovarian or breast carcinomas (Liang et al., 1992; Sager et al., 1993; Mok et al., 1994; Watson et al., 1994). By using arbitrary primers to generate “fingerprints” from total cell RNA, followed by separation of the amplified fragments by high resolution gel electrophoresis, it is possible to identify RNA species that are either up-regulated or down-regulated in cancer cells. Results of these studies indicated the presence of several markers of potential utility for diagnosis of breast or ovarian cancer, including a6-integrin (Sager et al., 1993), DEST001 and DEST002 (Watson et al., 1994), and LF4.0 (Mok et al., 1994).

[0017] There are two unique features of prostate cancer not shared by most of the other forms of human malignancies. First, the prevalence of prostate cancer is extremely high. In 1998 there are estimated to be 184,500 new cases diagnosed in American men accounting for nearly one-third of all male cancers (Parker et al., Cancer Journal for Clinicians. 47:5-27, 1997). At the same time there are predicted to be 39,000 deaths from prostate cancer or about 21% of the number of new cases. Prostate cancer is a disease of advancing years. By the sixth decade of life the chances of having prostate cancer are 1 in 5. In this group of men prostate cancer is the second most common form of death by cancer. But this is still only a fraction of those diagnosed. In contrast, the prevalence/incidence of lung cancer virtually equals the mortality from lung cancer with approximately 90,000 cases diagnosed and 90,000 deaths expected (Parker et al., Cancer Journal for Clinicians. 47:5-27, 1997; Boring et al., Cancer Journal for Clinicians. 44:7-26, 1994) and has remained unchanged for several years. The significant disparity between the total number of men diagnosed with prostate cancer and those dying from the disease emphasizes the importance of developing molecular markers to differentiate the virulent from indolent forms of prostate cancer and to help stratify management options for men presenting with prostate tumors. Current staging and prognostic modalities for human prostate cancer are woefully inadequate. Furthermore, our comprehension of the genetic influence over prostate carcinogenesis is lacking, although several genetic and epigenetic factors have been identified that correlate with the development of a more aggressive neoplastic phenotype (Bostwick et al., Journal of Cellular Biochemistry—Supplement. 19:283-289, 1994; Bostwick et al., Journal of Cellular Biochemistry, Supplement. 19:197-201, 1994; Rinker-Schaeffer et al., Cancer & Metastasis Reviews. 12:3-10,1993; Thompson et al., Genomics. 13:402-8,1992; Zhau et al., Journal of Cellular Biochemistry, Supplement. 19:208-216, 1994; Veltri et al., Journal of Cellular Biochemistry, Supplement. 19:249-258,1994). These include proliferation markers, pathophysiologic markers, growth factor-growth factor receptors, oncogenes, tumor suppressor genes, neuroendocrine products, and the extracellular matrix. These have been used either alone or in combination as prognostic and diagnostic markers. Unfortunately, these are poor markers and, to date, no single factor has been identified that can accurately predict the malignant potential of any given prostate tumor nor predict which patient with localized disease will eventually relapse or progress (Bostwick et al., Journal of Cellular Biochemistry—Supplement. 19: 283-289,1994;Veltri et al., Journal of Cellular Biochemistry, Supplement. 19: 249-258, 1994).

[0018] A second unique feature of prostate cancer is that it responds poorly to chemotherapy. Men with prostate cancer may initially respond well to hormonal or radiation therapy, but inevitably will relapse. Once an androgen independent phenotype is acquired, no effective therapies are currently available. For this reason, it is critically important to develop both novel management strategies and therapeutic modalities for the treatment of advanced prostate disease. One obstacle to studying human prostate cancer has been the long latency period, generally ≧25-35 years, that is required for the progression of prostate cancer from its latent morphologic forms to clinically-apparent disease. To overcome this long latency period, our laboratory has developed a human prostate cancer progression model utilizing the LNCaP cell line, a useful androgen responsive cell line as the starting material from which were generated an array of cell-lineage related sublines. This model has been shown to be relevant to human prostate cancer progression and mimics the pathophysiologic changes observed clinically as a tumor acquires increasingly metastatic and tumorigenic characteristics (Thalmann et al., Cancer Research. 54:2577-2581,1994; Wu et al., The International Journal of Cancer. Submitted October 1997.:, 1997; Wu et al., International Journal of Cancer. 57:406-12, 1994).

[0019] Recent studies have identified several recurring genetic changes in prostate cancer including, inter alia: allelic loss (particularly loss of chromosome 8p and 16q) (Bova, et al., 1993; Macoska et al., 1994; Carter et al., 1990); generalized DNA hypermethylation (Isaacs et al., 1994); point mutations or deletions of the retinoblastoma (Rb) and p53 genes (Bookstein et al., 1990a; Bookstein et al., 1990b; Isaacs et al., 1991); alterations in the level of certain cell-cell adhesion molecules (i.e., E-cadherin/alpha-catenin) (Carter et al., 1990; Morton et al., 1993; Umbas et al., 1992) and aneuploidy and aneusomy of chromosomes detected by fluorescence in situ hybridization (FISH), particularly chromosomes 7 and 8 Macoska et al., 1994; Visakorpi et al., 1994; Takahashi et al., 1994; Alcaraz et al., 1994).

[0020] The analysis of DNA content/ploidy using flow cytometry and FISH has been demonstrated to have utility predicting prostate cancer aggressiveness (Pearsons et al., 1993; Macoska et al., 1994; Visakorpi et al., 1994; Takahashi et al., 1994; Alcaraz et al., 1994; Pearsons et al., 1993), but these methods are expensive, time-consuming, and the latter methodology requires the construction of centromere-specific probes for analysis. Additionally, specific nuclear matrix proteins have been reported to be associated with prostate cancer. (Partin et al., 1993). However, these protein markers apparently do not distinguish between benign prostate hyperplasia and prostate cancer. Martin et al., 1993). Unfortunately, markers which cannot distinguish between benign and malignant prostate tumors are deemed to be of little value to urologists.

[0021] From the clinical perspective, successfully managing a prostate cancer patient is often a difficult task for the practicing urologist. Although clinicians examine tumor architecture, measure prostate-specific antigen (PSA) levels, and estimate tumor volume to help guide clinical decision-making, these currently available staging and prognostic modalities are insufficient. Studies performed on other types of cancers, such as testicular, liver and colon, have determined that these tumors can express gene products that are normally expressed only in the fetus during normal development of those organs. Some examples of these fetal proteins, also called oncofetal markers, include alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA). For testicular, liver, and colon tumors, AFP and CEA are commonly used in diagnosis, therapy, and for predicting and monitoring responses to treatment. Unfortunately, these particular markers are not applicable to the management of prostate cancer and, to date, no similar oncofetal gene(s) have been identified with any prognostic or diagnostic potential for prostate disease. It has been demonstrated that embryonic or fetal genes, such as the carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP), are frequently re-expressed in a spatially or temporally inappropriate manner during carcinogenesis. This aberrant expression has particular importance for tumor biology and therapy. Both CEA and AFP have provided significant contribution to the detection and management of germ cell, gastrointestinal and hepatobiliary cancers. Although this approach has demonstrated successful application to the diagnosis and treatment of the aforementioned tumors, no such correlates to these markers have been developed for prostate cancer.

[0022] As a result, there remain, however, deficiencies in the prior art with respect to the identification of the fetal genes linked with the progression of prostate cancer and the development of diagnostic methods to monitor disease progression. Likewise, the identification of fetal genes which are differentially expressed in prostate cancer would be of considerable importance in the development of a rapid, inexpensive method to diagnose prostate cancer. The present invention addresses the deficiencies in the prior art.

III. SUMMARY OF THE INVENTION

[0023] One aspect of the present invention is novel isolated nucleic acid segments that are useful as described herein as hybridization probes and primers that specifically hybridize to prostate disease markers. These disease markers, including both known genes and previously undescribed genes, are described herein as those fetal genes shown to be differentially expressed (either up- or down-regulated) in a prostate disease state as compared to a normal prostate. The novel isolated nucleic acid segments are designated herein as ug92, ug93, ug96, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194. The invention further comprises an isolated nucleic acid of between about 14 and about 100 bases in length, either identical to or complementary to a portion of the same length occurring within the disclosed sequences.

[0024] The present invention comprises proteins and peptides with amino acid sequences encoded by the aforementioned isolated nucleic acid segments. The invention also comprises methods for identifying biomarkers useful for prognostic or diagnostic assays of human prostate disease, and for identifying those fetal genes which are differentially expressed between prostate cancers versus normal or benign prostate.

[0025] The invention further comprises methods for detecting prostate cancer cells in biological samples, using hybridization primers and probes designed to specifically hybridize to prostate cancer markers. The hybridization probes are identified and designated herein as ug092, ug093,ug096,ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194. This method further comprises measuring the amounts of nucleic acid amplification products formed when primers selected from the designated sequences are used.

[0026] The invention further comprises the prognosis and/or diagnosis of prostate cancer by measuring the amounts of nucleic acid amplification products formed as above. The invention comprises methods of treating individuals with prostate cancer by providing effective amounts of substances, including, inter alia, antibodies and/or antisense DNA molecules which bind to the products of the above mentioned isolated nucleic acids. The invention further comprises kits for performing the above-mentioned procedures, containing amplification primers and/or hybridization probes.

[0027] The present invention further comprises production of antibodies specific for proteins or peptides encoded by ug092, ug093, ug096, ug101, ug102, ug106, ug 120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194, and the use of those antibodies for diagnostic applications in detecting diseases of the prostate, including, without limitation, prostatitis, and benign and malignant growth of the prostate gland.

[0028] The invention further comprises therapeutic treatment of diseases of the prostate, including, without limitation, prostatitis, and benign and malignant growth of the prostate gland by administration of pharmaceutically effective doses of inhibitors specific for proteins encoded by the aforementioned markers.

[0029] The invention further comprises therapeutic treatment of diseases of the prostate, including, without limitation, prostatitis, and benign and malignant growth of the prostate gland by the use of novel isolated nucleic acid segments comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194 for the development of therapeutic modalities including tissue-or cancer-specific gene promoters for use in gene therapy by naked DNA delivery or viral toxic gene therapy, growth suppression of prostate cancer by replacement gene therapy, and tissue specific gene products used to develop immunotherapeutic agents using peptide specific anti-prostate cancer vaccines or adoptive immunotherapies using peptide/protein specific cytotoxic T-cells.

IV. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The following drawings form part of the present specification and are included to further demonstrate certain aspects of the present invention. The invention may be understood better by reference to one or more of these drawings in combination with the detailed description of specific embodiments presented herein.

[0031]FIG. 1: Nucleotide sequences for 787 urogenital sinus (UGS)-derived ESTs

[0032]FIG. 2: Representative grid for columns “E”. The dot E1 is the result of pooled clones 297, 306, 314, 323, 333, 342, 352 and 360. Dot E16 on the matrix represents the addition of clones 297, 298, 299, 300, 301, 302, 304 and 305.

[0033]FIG. 3: Duplicate Dot Matrix Array filters spotted with 320 cDNA clones in pooled sets of 64 as depicted in FIG. 2 for set E. Each pair of columns A-E represents a new set of 64 clones in overlapping arrays. Radiolabeled cDNAs, reverse transcribed from LNCaP and C4-2 human prostate cancer cell line RNAs, were used as probes. The arrows indicate the pair of spots corresponding to UG311 as depicted in FIG. 2 that are lost with progression from LNCaP to C4-2.

[0034]FIG. 4: Northern blot analysis using the UG311 EST as a probe on a progression series of lineage-related prostate cancer cell lines either not-treated or treated for 48 h with 1 nM R1881 (androgen).

[0035]FIG. 5: Fold luciferase induction in LNCaP and C4-2 prostate cancer cell lines. Cells that are stably transfected with pTET-on were assayed by transient transfection to determine their ability to induce luciferase expression from pTRE-luc in response to doxycycline.

[0036]FIG. 6: RNA bolts using 30 μg total RNA from the cell lines as indicated. LNCaP through C4-2B#4 represent lineage-related cell lines having progressively more androgen independence and metastatic capacity. The +/− signs signify whether or not the samples were treated with 1 nM R1881 for 48 hours in serum-free conditions.

[0037]FIG. 7: Schematic representation of UGS-derived cDNA protein coding sequence into bacterial expression vector pGEX-4T for generating recombinant protein for use as immunogen.

[0038]FIG. 8: Urogenital sinus cDNA clone summary obtained from GelView Contig run: A determination of the range of independent sequences.

[0039]FIG. 9: Additional consensus sequence of differentially expressed cDNA clones.

V. DETAILED DESCRIPTION OF THE INVENTION

[0040] Current staging and prognostic modalities for human prostate cancer are woefully inadequate. Furthermore, the current comprehension of the genetic influence over prostate carcinogenesis is lacking, although several genetic and epigenetic factors have been identified that correlate with the development of a more aggressive neoplastic phenotype. In the human, mesenchymal-epithelial interaction maintains the functional integrity of the adult prostate gland. Prior investigations in our laboratory have demonstrated that fetal mesenchyme has the capacity to initiate glandular overgrowth of the adult rodent prostate (Sikes et al., Biology of Reproduction. 43: 353-62, 1990; Chung et al., Biology of Reproduction. 31:155-163, 1984), reduce anaplasia in the Dunning prostatic adenocarcinoma model (Chung et al., Prostate. 17:165-74, 1990; Hayashi et al., Cancer Research. 50: 4747-54, 1990), and induce the differentiation of androgen receptor deficient urogenital sinus epithelium (UGE) into functional prostate tissue (Chung et al., Biology of Reproduction. 31:155-163, 1984; Chung et al., Prostate. 17:165-74, 1990; Hayashi et al., Cancer Research. 50:4747-54, 1990; Chung et al., Molecular Biology Reports. 23:13-19, 1996. Prostatic carcinogenesis may be explained by aberrant instructive influences derived from its underlying stroma, as the microenvironment surrounding cancer epithelium has been demonstrated to determine tumor growth and malignant potential (Bissell et al., The Journal of Theoretical Biology. 99:31-68, 1982; Jacobson, Science. 152:25-34, 1966).

[0041] Consequently, it is believed that abnormal prostate growth and prostate carcinogenesis may result from abnormalities of the constituents of the stromal-epithehal milieu. The inductive role of stroma has been demonstrated in a wide variety of glandular tissues during embryonic development, including the prostate (Sakakura et al., Developmental Biology. 72:201-210, 1979; Drews et al., Cell. 10:401-404, 1977; Franks et al., The Journal of Pathology. 100:113-120, 1970; McNeal, Investigative Urology. 15:340-5,1978; Cunha et al., Journal of Steroid Biochemistry. 14:1317-24, 1981; Cunha et al., Biology of Reproduction. 22:19-42, 1980). Prostatic proliferation in the adult may result from a reawakening of dormant embryonic growth elements present in the prostatic stroma (Chung et al., Prostate. 4:503-11, 1983). It has been demonstrated that fetal urogenital sinus mesenchyme (UGM), a fetal form of prostatic stroma, is inductive and can redirect prostatic epithelial growth and differentiation (Chung et al., Biology of Reproduction. 31:155-163, 1984; Cunha et al., Endocrine Reviews. 8:338-62, 1987). Marked growth and expression of tissue-specific secretory proteins can be induced when fetal UGM is recombined with either fetal or adult prostate epithelium (Gleave et al., Cancer Research. 51:3753-61, 1991; Chung, Cancer Surveys. 23:33-42, 1995) or when it is implanted directly into the adult prostate gland (Evans, The Brittish Journal of Cancer. 68: 1051-1060,1993; Sokoloff et al., Cancer. 77:1862-1872,1996). Implanted fetal mesenchyme can induce differentiation and growth of adult rat urogenital cells (Chung et al., Prostate. 17:165-74, 1990; Hayashi et al., Cancer Research. 50:4747-54, 1990). Recombinants of androgen receptor deficient fetal mesenchyme with either fetal or adult epithelium failed to produce appropriate cytodifferentiation when recombined with fetal UGM lacking the androgen receptor (derived from testicular feminization, Tfm/y, fetuses)(Chung et al., Biology of Reproduction. 31:155-163, 1984; Chung, Cancer Surveys. 23:33-42, 1995). This further supports the contention that paracrine mediators between stroma and epithelium are prerequisite for prostate growth and morphogenesis.

[0042] Inductive influences from stroma to prostatic epithelial differentiation can be classified as either directive or permissive, depending upon the sources of embryonic epithelium and the age of both the inductive and responsive fetal tissue (Han et al., Carcinogenesis. 16:951-954, 1995). Thereafter, the ultimate growth potential of the embryonic and adult prostatic epithelium in tissue recombinants or in situ will be dictated by the presence of inductive stroma. By varying the amount of embryonic stroma used in the construction of tissue recombinants (Chung, Cancer Surveys. 23:33-42, 1995) or by inserting fetal UGM directly into the adult prostate (Evans, The Brittish Journal of Cancer. 68:1051-1060, 1993), the growth potential of prostatic epithelium is dictated entirely by the amount of UGM present in either tissue recombinants or in the induced chimeric adult gland. Hence, mesenchymal agents can induce normal and neoplastic prostate growth and differentiation. Furthermore, prostate carcinogenesis mimics a reversion to a more developmentally primitive state. Therefore, the differential expression of prostate-embryonic genes may direct neoplastic transformation or, at least, identify when a clonal population has undergone such transformation.

[0043] The temporal involvement of steroid hormones and growth factors is paramount to prostate development. Prostate growth and differentiation is tightly regulated by androgens and is influenced by a number of soluble peptide growth factors and their receptors (Cunha et al., Recent Progress in Hormone Research. 39:559-98, 1983). A close reciprocal association between stromal and epithelial tissues also has a fundamental role in normal, benign, and malignant prostate development. Mesenchymal and epithelial differentiation depends upon the stimulatory effects of dihydrotestosterone, inductive growth factors and peptides, and embryonic factors (Cunha et al., Recent Progress in Hormone Research. 39:559-98, 1983). The combination of epidermal growth factor, transforming growth factor-β, insulin growth factor, and gonadotropin can induce differentiation of reproductive cells. Other studies have demonstrated that many of the properties associated with tumor progression and metastasis in hormone-refractory prostate cancer cell lines can be altered after treatment with cytokines (Sokoloff et al., Cancer. 77:1862-1872, 1996; Ritchie et al., Endocrinology. 138:1145-1150, 1997). Suppression of prostate cancer cell growth correlated with the downregulation of oncogene, suppressor gene, growth factor, and adhesion molecule gene expression.

[0044] Our laboratory studies the interaction of prostate cancer cells and their surrounding environment, known as stroma. It has been shown that the stroma can alter normal prostate behavior and contribute to cancer progression. Furthermore, it has been shown that when normal prostate tissue is exposed to fetal tissue, the growth and development of the normal prostate resembles that of a neoplastic prostate. Many similarities exist between fetal tissue and neoplastic tissue. These include an increased rate of growth, the predilection to invade and migrate to distant locations as well as an inclination for undergoing internal changes that can detour a cell from maturing normally. These cells either remain underdeveloped or acquire the characteristics of a cell with non-prostate qualities or fetal prostate qualities.

[0045] In the human, mesenchymal-epithelial interaction maintains the functional integrity of the adult prostate gland. Indeed, some of the prognostic markers discussed previously, such as the extracellular matrix, basement membrane integrity and intermediate filament/integrin alterations, demonstrate that changes in the mesenchymal-epithelial interaction are hallmarks of cancer development. Prior investigations have demonstrated that fetal prostate mesenchyme has the capacity to initiate glandular overgrowth of adult rodent prostates (McKinnell et al., New York: Plenum Press, 1989; Pierce, N.J.: Prentiss-Hall, Inc., 1978; Sikes et al., Biology of Reproduction. 43:353-62, 1990; Chung et al., Biology of Reproduction. 31:155-163, 1984), reduce anaplasia in the Dunning prostatic adenocarcinoma model (Chung et al., Prostate. 17:165-74, 1990; Hayashi et al., Cancer Research. 50:4747-54, 1990), and induce the differentiation of androgen receptor deficient urogenital sinus epithelium into functional prostate tissue (Sikes et al., Biology of Reproduction. 43:353-62, 1990; Chung et al., Molecular Biology Reports.23:13-19,1996; Bissell et al., The Journal of Theoretical Biology. 99:31-68, 1982). As such, the instructive influence of fetal mesenchymal gene products to drive differentiation and growth is of particular interest for cancer biology since fetal tissues: divide rapidly, migrate and invade, remodel and differentiate; all of which are properties fetal tissues have in common with cancer cells. Additionally, many cancers have an embryonic appearance and express fetal (Jacobson, Science. 152:25-34, 1966) gene or differentiated (Sakakura et al., Developmental Biology. 72:201-210,1979) gene products in an inappropriate temporally or spatially manner.

[0046] Since there has been no examination of fetal prostate gene expression in prostate cancer, we sought to examine the possibility that UGS-derived gene products might be oncofetal markers for prostate cancer. Therefore, in order to investigate the role of gene expression during prostate embryogenesis and to then relate this to changes in gene expression during prostate cancer progression, a cDNA library was made from murine urogenital sinus (UGS), the prostate progenitor, and 787 clones were generated and randomly screened. Of these 787 cDNA clones, 728 generated useful sequence information. These 728 fetal murine urogenital sinus (UGS)-derived cDNA clones were subsequently screened for their expression in the LNCaP (androgen dependent, non-tumorigenic) and lineage derived C4-2 (androgen-independent, tumorigenic metastatic to bone) cell lines that closely mimic the natural progression of human prostate cancer but in a much shorter time frame. This model allows the comparison of the 728 UGS derived cDNA clones with the expressed genes from a less-aggressive versus a more aggressive prostate cancer model. This screen has identified over 33 UGS expressed sequence tags or cDNA clones whose level of expression changes when the androgen sensitive LNCaP probed filters are compared to the androgen independent C4-2 clone probed filters.

[0047] This represents the first documented evidence that fetal urogenital sinus-derived genes have been associated with the malignant potential of prostate cancer. This evidence immediately suggests that fetal prostate gene expression or loss in the prostate is significant in the development and progression of prostate cancer. In addition to clarifying the role of embryonic influences on prostate carcinogenesis, these differentially-expressed genes can also be developed into prognostic markers and targets for gene therapy and other therapeutic modalities to detect and prevent the development and progression of human prostate cancer. Furthermore, such gene products encoded by these genes can also be used to predict a prostate cancer's aggressiveness and to differentiate prostate cancers exhibiting different degrees of virulence. Such an approach has never before been employed with fetal prostate genes and thus represents a novel approach to diagnosis of prostate cancer. The methods employed herein may thus be used to examine those fetal genes which show the greatest change in expression and to develop improved techniques of monitoring patients with prostate cancer and novel therapies to prevent or retard cancerous changes in the prostate. Both of these advances should make a significant impact on the clinical management of men with prostate disease.

[0048] The more than 780 randomly screened fetal murine urogenital sinus (UGS)-derived cDNA clones described above have the following designations: ua1a2 (SEQ ID NO:1); ua1a4f (SEQ ID NO:2); ua1a4r (SEQ ID NO:3 ); ua1a6f (SEQ ID NO:4 ); ua1a6r (SEQ ID NO:5 ); ua1b4f (SEQ ID NO:6); ua1b4r (SEQ ID NO:7); ua1b5 (SEQ ID NO:8); ua1d1 (SEQ ID NO:9); ua1c6f (SEQ ID NO:10); ua1c6r (SEQ ID NO:11); ua1c6r (SEQ ID NO:12); ua1d2 (SEQ ID NO:13); ua1d4 (SEQ ID NO:14); ua1e1f (SEQ ID NO:15); ua1e1r (SEQ ID NO: 16); ua1e3f (SEQ ID NO:17); ua1e3r (SEQ ID NO:18); ua1e5r (SEQ ID NO:19); ua1e6f (SEQ ID NO:20); ua1e6r (SEQ ID NO:21); ua1f1r (SEQ ID NO:22); ua1f3f (SEQ ID NO: 23); ua1f3r (SEQ ID NO:24); ua1f4f (SEQ ID NO:25); ua1f5f (SEQ ID NO:26); ua1f6f (SEQ ID NO:27); ua1f6r (SEQ ID NO:28); ua1g2f (SEQ ID NO:29); ua1g4r (SEQ ID NO:30); ua1g5f (SEQ ID NO:31); ua1h2f (SEQ ID NO:32); ua1h3f (SEQ ID NO:33); ua1h4 (SEQ ID NO:34); ua2h6f (SEQ ID NO:35); ua2h6r (SEQ ID NO:36); ua2h6f (SEQ ID NO:37); ua2h6r (SEQ ID NO:38); ua2h7r (SEQ ID NO:39); ug1rcon (SEQ ID NO:40); ug2rcon (SEQ ID NO: 41); ug3 meld (SEQ ID NO:42); ug4rcon (SEQ ID NO:43); ug5rcon (SEQ ID NO:44); ug6rcon (SEQ ID NO:45); ug6?con (SEQ ID NO:46); ug7rcon (SEQ ID NO:47); ug8rcon (SEQ ID NO:48); ug9rcon (SEQ ID NO:49); ug10rcon (SEQ ID NO:50); ug11rcon (SEQ ID NO:51); ug12rcon (SEQ ID NO:52); ug13rcon (SEQ ID NO:53); ug14rcon (SEQ ID NO:54); ug15rcon (SEQ ID NO:55); ug16/38180 (SEQ ID NO:56); ug17rcon (SEQ ID NO:57); ug18reon (SEQ ID NO:58); ug19rcon (SEQ ID NO:59); ug20r2 (SEQ ID NO:60); ug21rcon (SEQ ID NO:61); ug22rcon (SEQ ID NO:62); ug23rcon (SEQ ID NO:63); ug24rcon (SEQ ID NO:64); ug25rcon (SEQ ID NO:65); ug26rcon (SEQ ID NO:66); ug27rcon (SEQ ID NO: 67); ug28rcon (SEQ ID NO:68); ug29rcon (SEQ ID NO:69); ug30rcon (SEQ ID NO:70); ug31con (SEQ ID NO:71); ug32rcon (SEQ ID NO:72); ug33con (SEQ ID NO:73); ug34con (SEQ ID NO:74); ug35con (SEQ ID NO:75); ug36rcon (SEQ ID NO:76); ug37rcon (SEQ ID NO:77); ug39rcon (SEQ ID NO:78); ug42rcon (SEQ ID NO:79); ug43rcon (SEQ ID NO: 80); ug42con (SEQ ID NO:81); ug43rcon (SEQ ID NO:82); ug44rcon (SEQ ID NO:83); ug45 (SEQ ID NO:84); ug46 (SEQ ID NO:85); ug47rcon (SEQ ID NO:86); ug48 (SEQ ID NO: 87); ug49rcon (SEQ ID NO:88); ug50rcon (SEQ ID NO:89); ugs51rcon (SEQ ID NO:90); ug52rcon (SEQ ID NO:91); ug53rcon (SEQ ID NO:92); ug54 (SEQ ID NO:93); ug53rcon (SEQ ID NO:94); ug56 (SEQ ID NO:95); ug 57rcon (SEQ ID NO:96); ug58rcon (SEQ ID NO:97); ug59 (SEQ ID NO:98); ug60 (SEQ ID NO:99); ug61rcon (SEQ ID NO:100); ug62rcon (SEQ ID NO:101); ug63rcon (SEQ ID NO:102); ug64rcon (SEQ ID NO:103); ug65rcon (SEQ ID NO:104); ug66rcon (SEQ ID NO:105); ug67rcon (SEQ ID NO:106); ug68rcon (SEQ ID NO:107); ug69rcon (SEQ ID NO:108); ug70rcon (SEQ ID NO:109); ug71rcon (SEQ ID NO:110); ug72rcon (SEQ ID NO:111); ug73rcon (SEQ ID NO:112); ug74rcon (SEQ ID NO:113); ug75rcon (SEQ ID NO:114); ug76rcon (SEQ ID NO:115); ug77rcon (SEQ ID NO:116); ug78rcon (SEQ ID NO:117); ug79rcon (SEQ ID NO:118); ug81rcon (SEQ ID NO:119); ug82rcon (SEQ ID NO:120); ug83rcon (SEQ ID NO:121); ug84rcon (SEQ ID NO:122); ug85rcon (SEQ ID NO:123); ug86rcon (SEQ ID NO:124); ug87rcon (SEQ ID NO:125); ug88rcon (SEQ ID NO:126); ug89rcon (SEQ ID NO:127); ug90rcon (SEQ ID NO:128); ug91rcon (SEQ ID NO:129); ug92rcon (SEQ ID NO:130); ug93rcon (SEQ ID NO:131); ug94rcon (SEQ ID NO:132); ug95rcon (SEQ ID NO:133); ug96 (SEQ ID NO:134); ug96rcon (SEQ ID NO:135); ug97rcon (SEQ ID NO:136); ug98rcon (SEQ ID NO:137); ug99rcon (SEQ ID NO:138); ug100rcon (SEQ ID NO:139); ug101rcon (SEQ ID NO:140); ug102rcon (SEQ ID NO:141); ug103rcon (SEQ ID NO:142); ug104rcon (SEQ ID NO:143); ug106rcon (SEQ ID NO:144); ug107rcon (SEQ ID NO:145); ug108rcon (SEQ ID NO:146); ug109rcon (SEQ ID NO:147); ug110rcon (SEQ ID NO:148); ug111rcon (SEQ ID NO:149); ug112 (SEQ ID NO:150); ug113rcon (SEQ ID NO:151); ug114rcon (SEQ ID NO:152); ug115rcn1o (SEQ ID NO:153); ug115rcon (SEQ ID NO:154); ug116rcon (SEQ ID NO:155); ug117 (SEQ ID NO:156); ug118 (SEQ ID NO:157); ug119 (SEQ ID NO:158); ug120rcon (SEQ ID NO:159); ug121(SEQ ID NO:160); ug122rcon (SEQ ID NO:161); ug123 (SEQ ID NO:162); ug124 (SEQ ID NO:163); ug125 (SEQ ID NO:164); ug126 (SEQ ID NO:165); ug127 (SEQ ID NO:166); ug128 (SEQ ID NO:167); ug129 (SEQ ID NO:168); ug130 (SEQ ID NO:169); ug130r2 (SEQ ID NO:170);ug131 (SEQ ID NO:171); ug132 (SEQ ID NO:172); ug133 (SEQ ID NO:173); ug134 (SEQ ID NO:174); ug135 (SEQ ID NO:175); ug136rcon (SEQ ID NO:176); ug137rcon (SEQ ID NO:177); ug138 (SEQ ID NO:178); ug139 (SEQ ID NO:179); ug140 (SEQ ID NO:180); ug141rcon (SEQ ID NO:181); ug142 (SEQ ID NO:182); ug143 (SEQ ID NO:183); ug144 (SEQ ID NO:184); ug145 (SEQ ID NO: 185); ug146 (SEQ ID NO:186); ug147 (SEQ ID NO:187); ug148 (SEQ ID NO:188); ug149rcon (SEQ ID NO:189); ug150rcon (SEQ ID NO:190); ug151rcon (SEQ ID NO:191); ug152rcon (SEQ ID NO:192); ug153rcon (SEQ ID NO:193); ug154rcon (SEQ ID NO:194); ug155rcon (SEQ ID NO:195); ug156rcon (SEQ ID NO:196); ug157rcon (SEQ ID NO:197); ug158 (SEQ ID NO:198); ug159 (SEQ ID NO:199); ug160 (SEQ ID NO:200); ug161 (SEQ ID NO:201); ug162 (SEQ ID NO:202); ug163rcon (SEQ ID NO:203); ug164rcon (SEQ ID NO:204); ug165rcon (SEQ ID NO:205); ug166rcon (SEQ ID NO:206); ug167rcon (SEQ ID NO:207); ug168rcon (SEQ ID NO:208); ug169rcon (SEQ ID NO:209); ug170rcon (SEQ ID NO:210); ug171rcon (SEQ ID NO:211); ug172rcon (SEQ ID NO:212); ug173rcon (SEQ ID NO:213); ug174rcon (SEQ ID NO:214); ug175rcon (SEQ ID NO:215); ug176rcon (SEQ ID NO:216); ug177rcon (SEQ ID NO:217); ug178rcon (SEQ ID NO:218); ug179rcon (SEQ ID NO:219); ug180rcon (SEQ ID NO:220); ug181rcon (SEQ ID NO:221); ug182 (SEQ ID NO: 222); ug183rcon (SEQ ID NO:223); ug184rcon (SEQ ID NO:224); ug185 (SEQ ID NO:225); ug185rcon (SEQ ID NO:226); ug186rcon (SEQ ID NO:227); ug187rcon (SEQ ID NO:228); ug188rcon (SEQ ID NO:229); ug189rcon (SEQ ID NO:230); ug190rcon (SEQ ID NO:231); ug191rcon (SEQ ID NO:232); ug192rcon (SEQ ID NO:233); ug193 (SEQ ID NO:234); ug194 (SEQ ID NO:235); ug195 (SEQ ID NO:236); ug196 (SEQ ID NO:237); ug197 (SEQ ID NO:238); ug198 (SEQ ID NO:239); ug199 (SEQ ID NO:240); ug200 (SEQ ID NO:241); ug201 (SEQ ID NO:242); ug202 (SEQ ID NO:243); ug203 (SEQ ID NO:244); ug204 (SEQ ID NO:245); ug205 (SEQ ID NO:246); ug206 (SEQ ID NO:247); ug207 (SEQ ID NO:248); ug208 (SEQ ID NO:249); ug209 (SEQ ID NO:250); ug210 (SEQ ID NO:251); ug210 (SEQ ID NO:252); ug211 (SEQ ID NO:253); ug212 (SEQ ID NO:254); ug213 (SEQ ID NO:255); ug214 (SEQ ID NO:256); ug215 (SEQ ID NO:257); ug216 (SEQ ID NO:258); ug217 (SEQ ID NO:259); ug218 (SEQ ID NO:260); ug219 (SEQ ID NO:261); ug220 (SEQ ID NO:262); ug221 (SEQ ID NO:263); ug222 (SEQ ID NO:264); ug223 (SEQ ID NO:265); ug224 (SEQ ID NO:266); ug225 (SEQ ID NO:267); ug226 (SEQ ID NO:268); ug227 (SEQ ID NO:269); ug228 (SEQ ID NO:270); ug229 (SEQ ID NO:271); ug230 (SEQ ID NO:272); ug231 (SEQ ID NO:273); ug232 (SEQ ID NO:274); ug233 (SEQ ID NO:275); ug234 (SEQ ID NO:276); ug235 (SEQ ID NO:277); ug236 (SEQ ID NO:278); ug237 (SEQ ID NO:279); ug238 (SEQ ID NO:280); ug239 (SEQ ID NO:281); ug240 (SEQ ID NO:282); ug241 (SEQ ID NO:283); ug242 (SEQ ID NO:284); ug243 (SEQ ID NO:285); ug244 (SEQ ID NO:286); ug245 (SEQ ID NO:287); ug246 (SEQ ID NO:288); ug247 (SEQ ID NO:289); ug248 (SEQ ID NO:290); ug249 (SEQ ID NO:291); ug250 (SEQ ID NO:292); ug251 (SEQ ID NO:293); ug252 (SEQ ID NO:294); ug253 (SEQ ID NO:295); ug254 (SEQ ID NO:296); ug255 (SEQ ID NO:297); ug256 (SEQ ID NO:298); ug257 (SEQ ID NO:299); ug258 (SEQ ID NO:300); ug259 (SEQ ID NO:301); ug260 (SEQ ID NO:302); ug261 (SEQ ID NO:303); ug262 (SEQ ID NO:304); ug263 (SEQ ID NO:305); ug264 (SEQ ID NO:306); ug265 (SEQ ID NO:307); ug266 (SEQ ID NO:308); ug267 (SEQ ID NO:309); ug268 (SEQ ID NO:310); ug269 (SEQ ID NO:311); ug270 (SEQ ID NO:312); ug271 (SEQ ID NO:313); ug272 (SEQ ID NO:314); ug273 (SEQ ID NO:315); ug274 (SEQ ID NO:316); ug275 (SEQ ID NO:317); ug276 (SEQ ID NO:318); ug277 (SEQ ID NO:319); ug278 (SEQ ID NO:320); ug279 (SEQ ID NO:321); ug280 (SEQ ID NO:322); ug281 (SEQ ID NO:323); ug282 (SEQ ID NO:324); ug283 (SEQ ID NO:325); ug284 (SEQ ID NO:326); ug285 (SEQ ID NO:327); ug286 (SEQ ID NO:328); ug287 (SEQ ID NO:329); ug288 (SEQ ID NO:330); ug289 (SEQ ID NO:331); ug290 (SEQ ID NO:332); ug291 (SEQ ID NO:333); ug292 (SEQ ID NO:334); ug293 (SEQ ID NO:335); ug294 (SEQ ID NO:336); ug295 (SEQ ID NO:337); ug296 (SEQ ID NO:338); ug297 (SEQ ID NO:339); ug298 (SEQ ID NO:340); ug299 (SEQ ID NO:341); ug300 (SEQ ID NO:342); ug301 (SEQ ID NO:343); ug303 (SEQ ID NO:344); ug304 (SEQ ID NO:345); ug305 (SEQ ID NO:346); ug306 (SEQ ID NO:347); ug307 (SEQ ID NO:348); ug308 (SEQ ID NO:349); ug309 (SEQ ID NO:350); ug310 (SEQ ID NO:351); ug311 (SEQ ID NO:352); ug312 (SEQ ID NO:353); ug313 (SEQ ID NO:354); ug314 (SEQ ID NO:355); ug315 (SEQ ID NO:356); ug316 (SEQ ID NO:357); ug317 (SEQ ID NO:358); ug318 (SEQ ID NO:359); ug320 (SEQ ID NO:360); ug321 (SEQ ID NO:361); ug322 (SEQ ID NO:362); ug323 (SEQ ID NO:363); ug324 (SEQ ID NO:364); ug325 (SEQ ID NO:365); ug326 (SEQ ID NO:366); ug327 (SEQ ID NO:367); ug328 (SEQ ID NO:368); ug329 (SEQ ID NO:369); ug330 (SEQ ID NO:370); ug331 (SEQ ID NO:371); ug332 (SEQ ID NO:372); ug333 (SEQ ID NO:373); ug334 (SEQ ID NO:374); ug335 (SEQ ID NO:375); ug336 (SEQ ID NO:376); ug337 (SEQ ID NO:377); ug338 (SEQ ID NO:378); ug339 (SEQ ID NO:379); ug340 (SEQ ID NO:380); ug341 (SEQ ID NO:381); ug342 (SEQ ID NO:382); ug343 (SEQ ID NO:383); ug344 (SEQ ID NO:384); ug345 (SEQ ID NO:385); ug346 (SEQ ID NO:386); ug347 (SEQ ID NO:387); ug348 (SEQ ID NO:388); ug349 (SEQ ID NO:389); ug350 (SEQ ID NO:390); ug351 (SEQ ID NO:391); ug352 (SEQ ID NO:392); ug353 (SEQ ID NO:393); ug354 (SEQ ID NO:394); ug355 (SEQ ID NO:395); ug356 (SEQ ID NO:396); ug357 (SEQ ID NO:397); ug358 (SEQ ID NO:398); ug359 (SEQ ID NO:399); ug360 (SEQ ID NO:400); ug361 (SEQ ID NO:401); ug362 (SEQ ID NO:402); ug363 (SEQ ID NO:403); ug364 (SEQ ID NO:404); ug365 (SEQ ID NO:405); ug366 (SEQ ID NO:406); ug367 (SEQ ID NO:407); ug368 (SEQ ID NO:408); ug369 (SEQ ID NO:409); ug370 (SEQ ID NO:410); ug371 (SEQ ID NO:411); ug372 (SEQ ID NO:412); ug373 (SEQ ID NO:413); ug374 (SEQ ID NO:414); ug375 (SEQ ID NO:415); ug376 (SEQ ID NO:416); ug377 (SEQ ID NO:417); ug378 (SEQ ID NO:418); ug379 (SEQ ID NO:419); ug380 (SEQ ID NO:420); ug381 (SEQ ID NO:421); ug382 (SEQ ID NO:422); ug383 (SEQ ID NO:423); ug384 (SEQ ID NO:424); ug385 (SEQ ID NO:425); ug386 (SEQ ID NO:426); ug387 (SEQ ID NO:427); ug388 (SEQ ID NO:428); ug389 (SEQ ID NO:429); ug390 (SEQ ID NO:430); ug391 (SEQ ID NO:431); ug392 (SEQ ID NO:432); ug393 (SEQ ID NO:433); ug394 (SEQ ID NO:434); ug395 (SEQ ID NO:435); ug386 (SEQ ID NO:436); ug397 (SEQ ID NO:437); ug398 (SEQ ID NO:438); ug399 (SEQ ID NO:439); ug400 (SEQ ID NO:440); ug401 (SEQ ID NO:441); ug402 (SEQ ID NO:442); ug403 (SEQ ID NO:443); ug404 (SEQ ID NO:444); ug406 (SEQ ID NO:445); ug407 (SEQ ID NO:446); ug408 (SEQ ID NO:447); ug411 (SEQ ID NO:448); ug412 (SEQ ID NO:449); ug413 (SEQ ID NO:450); ug414 (SEQ ID NO:451); ug415 (SEQ ID NO:452); ug416 (SEQ ID NO:453); ug417 (SEQ ID NO:454); ug418 (SEQ ID NO:455); ug420 (SEQ ID NO:456); ug421 (SEQ ID NO:457); ug422 (SEQ ID NO:458); ug423 (SEQ ID NO:459); ug424 (SEQ ID NO:460); ug425 (SEQ ID NO:461); ug426 (SEQ ID NO:462); ug427 (SEQ ID NO:463); ug428 (SEQ ID NO:464); ug429 (SEQ ID NO:465); ug430 (SEQ ID NO:466); ug431 (SEQ ID NO:467); ug432 (SEQ ID NO:468); ug433 (SEQ ID NO:469); ug434 (SEQ ID NO:470); ug435 (SEQ ID NO:471); ug436 (SEQ ID NO:472); ug437 (SEQ ID NO:473); ug439 (SEQ ID NO:474); ug441 (SEQ ID NO:475); ug442 (SEQ ID NO:476); ug443 (SEQ ID NO:477); ug444 (SEQ ID NO:478); ug445 (SEQ ID NO:479); ug446 (SEQ ID NO:480); ug447 (SEQ ID NO:481); ug448 (SEQ ID NO:482); ug449 (SEQ ID NO:483); ug450 (SEQ ID NO:484); ug451 (SEQ ID NO:485); ug452 (SEQ ID NO:486); ug453 (SEQ ID NO:487); ug454 (SEQ ID NO:488); ug455 (SEQ ID NO:489); ug456 (SEQ ID NO:490); ug457 (SEQ ID NO:491); ug458 (SEQ ID NO:492); ug459 (SEQ ID NO:493); ug460 (SEQ ID NO:494); ug461 (SEQ ID NO:495); ug462 (SEQ ID NO:496); ug463 (SEQ ID NO:497); ug464 (SEQ ID NO:498); ug465 (SEQ ID NO:499); ug466 (SEQ ID NO:500); ug467 (SEQ ID NO:501); ug468 (SEQ ID NO:502); ug470 (SEQ ID NO:503); ug471 (SEQ ID NO:504); ug472 (SEQ ID NO:505); ug473 (SEQ ID NO:506); ug474 (SEQ ID NO:507); ug475 (SEQ ID NO:508); ug476 (SEQ ID NO:509); ug477 (SEQ ID NO:510); ug478 (SEQ ID NO:511); ug479 (SEQ ID NO:512); ug480 (SEQ ID NO:513); ug481 (SEQ ID NO:514); ug482 (SEQ ID NO:515); ug483 (SEQ ID NO:516); ug484 (SEQ ID NO:517); ug485 (SEQ ID NO:518); ug486 (SEQ ID NO:519); ug487 (SEQ ID NO:520); ug488 (SEQ ID NO:521); ug489 (SEQ ID NO:522); ug491 (SEQ ID NO:523); ug492 (SEQ ID NO:524); ug493 (SEQ ID NO:525); ug494 (SEQ ID NO:526); ug495 (SEQ ID NO:527); ug496 (SEQ ID NO:528); ug497 (SEQ ID NO:529); ug498 (SEQ ID NO:530); ug499 (SEQ ID NO:531); ug500 (SEQ ID NO:532); ug501 (SEQ ID NO:533); ug502 (SEQ ID NO:534); ug504 (SEQ ID NO:535); ug505 (SEQ ID NO:536); ug506 (SEQ ID NO:537); ug507 (SEQ ID NO:538); ug508 (SEQ ID NO:539); ug509 (SEQ ID NO:540); ug510 (SEQ ID NO:541); ug511 (SEQ ID NO:542); ug514 (SEQ ID NO:543); ug516 (SEQ ID NO:544); ug517 (SEQ ID NO:545); ug518 (SEQ ID NO:546); ug519 (SEQ ID NO:547); ug520 (SEQ ID NO:548); ug521 (SEQ ID NO:549); ug522 (SEQ ID NO:550); ug523 (SEQ ID NO:551); ug524 (SEQ ID NO:552); ug525 (SEQ ID NO:553); ugs001 (SEQ ID NO:554); ugs003 (SEQ ID NO:555); ugs005 (SEQ ID NO: 556); ugs006 (SEQ ID NO:557); ugs007 (SEQ ID NO:558); ugs008 (SEQ ID NO:559); ugs009 (SEQ ID NO:560); ugs010 (SEQ ID NO:561); ugs011 (SEQ ID NO:562); ugs012 (SEQ ID NO:563); ugs013 (SEQ ID NO:564); ugs014 (SEQ ID NO:565); ugs015) (SEQ ID NO:566); ugs016 (SEQ ID NO:567); ugs017 (SEQ ID NO:568); ugs018 (SEQ ID NO:569); ugs019 (SEQ ID NO:570); ugs020 (SEQ ID NO:571); ugs021 (SEQ ID NO:572); ugs022 (SEQ ID NO:573); ugs023 (SEQ ID NO:574); ugs024 (SEQ ID NO:575); ugs025 (SEQ ID NO:576); ugs026 (SEQ ID NO:577); ugs027 (SEQ ID NO:578); ugs028 (SEQ ID NO:579); ugs029 (SEQ ID NO:580); ugs030 (SEQ ID NO:581); ugs031 (SEQ ID NO:582); ugs032 (SEQ ID NO:583); ugs033 (SEQ ID NO:584); ugs034 (SEQ ID NO:585); ugs035 (SEQ ID NO:586); ugs036 (SEQ ID NO:587); ugs038 (SEQ ID NO:588); ugs039 (SEQ ID NO:589); ugs040 (SEQ ID NO:590); ugs041 (SEQ ID NO:591); ugs042 (SEQ ID NO:592); ugs043 (SEQ ID NO:593); ugs044 (SEQ ID NO:594); ugs045 (SEQ ID NO:595); ugs046 (SEQ ID NO:596); ugs047 (SEQ ID NO:597); ugs048 (SEQ ID NO:598); ugs050 (SEQ ID NO:599); ugs051 (SEQ ID NO:600); ugs052 (SEQ ID NO:601); ugs054 (SEQ ID NO:602); ugs055 (SEQ ID NO:603); ugs059 (SEQ ID NO:604); ugs060 (SEQ ID NO:605); ugs063 (SEQ ID NO:606); ugs064 (SEQ ID NO:607); ugs065 (SEQ ID NO:608); ugs066 (SEQ ID NO:609); ugs067 (SEQ ID NO:610); ugs068 (SEQ ID NO:611); ugs070 (SEQ ID NO:612); ugs071 (SEQ ID NO:613); ugs072 (SEQ ID NO:614); ugs074 (SEQ ID NO:615); ugs077 (SEQ ID NO:616); ugs078 (SEQ ID NO:617); ugs080 (SEQ ID NO:618); ugs084 (SEQ ID NO:619); ugs085 (SEQ ID NO:620); ugs086 (SEQ ID NO:621); ugs087 (SEQ ID NO:622); ugs088 (SEQ ID NO:623); ugs090 (SEQ ID NO:624); ugs091 (SEQ ID NO:625); ugs092 (SEQ ID NO:626); ugs093 (SEQ ID NO:627); ugs094 (SEQ ID NO:628); ugs095 (SEQ ID NO:629); ugs099 (SEQ ID NO:630); ugs100 (SEQ ID NO:631); ugs101 (SEQ ID NO:632); ugs102 (SEQ ID NO:633); ugs103 (SEQ ID NO:634); ugs104 (SEQ ID NO:635); ugs105 (SEQ ID NO:636); ugs106 (SEQ ID NO:637); ugs107 (SEQ ID NO:638); ugs108 (SEQ ID NO:639); ugs110 (SEQ ID NO:640); ugs111 (SEQ ID NO:641); ugs112 (SEQ ID NO:642); ugs113 (SEQ ID NO:643); ugs114 (SEQ ID NO:644); ugs115 (SEQ ID NO:645); ugs116 (SEQ ID NO:646); ugs117 (SEQ ID NO:647); ugs118 (SEQ ID NO:648); ugs119 (SEQ ID NO:649); ugs120 (SEQ ID NO:650); ugs121 (SEQ ID NO:651); ugs122 (SEQ ID NO:652); ugs123 (SEQ ID NO:653); ugs125 (SEQ ID NO:654); ugs126 (SEQ ID NO:655); ugs127 (SEQ ID NO:656); ugs128 (SEQ ID NO:657); ugs129 (SEQ ID NO:658); ugs131 (SEQ ID NO:659); ugs133 (SEQ ID NO:660); ugs134 (SEQ ID NO:661); ugs135 (SEQ ID NO:662); ugs136 (SEQ ID NO:663); ugs137 (SEQ ID NO:664); ugs138 (SEQ ID NO:665); ugs139 (SEQ ID NO:666); ugs140 (SEQ ID NO:667); ugs142 (SEQ ID NO:668); ugs143 (SEQ ID NO:669); ugs144 (SEQ ID NO:670); ugs145 (SEQ ID NO:671); ugs146 (SEQ ID NO:672); ugs147 (SEQ ID NO:673); ugs148 (SEQ ID NO:674); ugs149 (SEQ ID NO:675); ugs150 (SEQ ID NO:676); ugs151 (SEQ ID NO:677); ugs152 (SEQ ID NO:678); ugs153 (SEQ ID NO:679); ugs156 (SEQ ID NO:680); ugs157 (SEQ ID NO:681); ugs159 (SEQ ID NO:682); ugs160 (SEQ ID NO:683); ugs161 (SEQ ID NO:684); ugs163 (SEQ ID NO:685); ugs164 (SEQ ID NO:686); ugs165 (SEQ ID NO:687); ugs167 (SEQ ID NO:688); ugs168 (SEQ ID NO:689); ugs172 (SEQ ID NO:690); ugs173 (SEQ ID NO:691); ugs174 (SEQ ID NO:692); ugs175 (SEQ ID NO:693); ugs177 (SEQ ID NO:694); ugs178 (SEQ ID NO:695); ugs179 (SEQ ID NO:696); ugs180 (SEQ ID NO:697); ugs181 (SEQ ID NO:698); ugs182 (SEQ ID NO:699); ugs183 (SEQ ID NO:700); ugs184 (SEQ ID NO:701); ugs186 (SEQ ID NO:702); ugs187 (SEQ ID NO:703); ugs188 (SEQ ID NO:704); ugs190 (SEQ ID NO:705); ugs191 (SEQ ID NO:706); ugs192 (SEQ ID NO:707); ugs193 (SEQ ID NO:708); ugs194 (SEQ ID NO:709); ugs195 (SEQ ID NO:710); ugs196 (SEQ ID NO:711); ugs198 (SEQ ID NO:712); ugs199 (SEQ ID NO:713); ugs200 (SEQ ID NO:714); ugs201 (SEQ ID NO:715); ugs202 (SEQ ID NO:716); ugs203 (SEQ ID NO:717); ugs204 (SEQ ID NO:718); ugs205 (SEQ ID NO:719); ugs206 (SEQ ID NO:720); ugs208 (SEQ ID NO:721); ugs210 (SEQ ID NO:722); ugs211 (SEQ ID NO:723); ugs212 (SEQ ID NO:724); ugs213 (SEQ ID NO:725); ugs214 (SEQ ID NO:726); ugs216 (SEQ ID NO:727); ugs217 (SEQ ID NO:728); ugs218 (SEQ ID NO:729); ugs219 (SEQ ID NO:730); ugs221 (SEQ ID NO:731); ugs223 (SEQ ID NO:732); ugs225 (SEQ ID NO:733); ugs226 (SEQ ID NO:734); ugs227 (SEQ ID NO:735); ugs228 (SEQ ID NO:736); ugs229 (SEQ ID NO:737); ugs231 (SEQ ID NO:738); ugs232 (SEQ ID NO:739); ugs233 (SEQ ID NO:740); ugs234 (SEQ ID NO:741); ugs235 (SEQ ID NO:742); and ugs236 (SEQ ID NO:743).

[0049] The 728 cloned and sequenced urogenital sinus expressed sequence tags (ESTs), representing 787 identified bacterial clones, total more than 330,000 bp of nucleotide sequence. These ESTs were first compared to the GenBank database with the following results: unique=64%; Known=28%; Moderate homology=5% with 3% vector sequences. The high complexity of the fetal library is comparable to the fetal heart findings of CC Lieu in Toronto. In order to narrow the focus to those fetal genes expressed during prostate cancer progression, a matrix blot format was developed (FIG. 1). In this format it is possible to screen 384 clones per filter using a 8×12 dot blot apparatus. The data obtained for 320 clones is depicted in FIG. 2. Using this grid matrix and probing duplicate filters simultaneously with LNCaP and C4-2 ³²p labeled cDNAs, 33 clones were identified from all 728 ESTs examined whose expression levels change dramatically between the two cell lines. The arrows shown in FIG. 2 indicate a pair of spots where the level of expression between LNCaP and C4-2 has dropped remarkably. By following the clone grid (FIG. 1, underlined) for the two E columns one can locate the spots corresponding to the increased signals. A clone's level of expression must change in at least two spots and be confirmed by RNA blot to be identified as increased (up-regulated) or decreased (down-regulated). As can be seen in the northern blot shown in FIG. 3, the clone designated UG311 has an elevated expression in LNCaP that decreases with increasing malignant potential, e.g. C4-2 by 5-7 fold. This particular clone is not regulated by androgens. In a similar fashion, other clones have been identified from these duplicate blots which are up regulated from the LNCaP to C4-2 in the human prostate cancer progression model. For example, FIG. 6 depicts the Northern blot for ug494, as well as for ug311, using the LNCaP (androgen dependent, non-tumorigenic) and lineage derived C4-2 (androgen-independent, tumorigenic metastatic to bone) cell line model. FIG. 6 shows that the fetal gene-derived EST ug494 is up-regulated in the C4-2 cell line compared to the LNCaP progression prostate cancer model cell line. These results form the basis of the experimental design described in more detail in Example 1 to completely characterize the UG311 EST by cloning and expressing UG311 EST in both bacterial systems for antibody development and in mammalian cell lines to determine their ability to modify the behavior of the LNCaP-C4-2 human prostate cancer progression model.

[0050] Tables 1-7 represent the subtractive analysis of homology determinations for all of the 728 cDNA clones as performed against the various databases. The asterisk represents a potentially differentially expressed UGS cDNA clone. See Table 7 for a summary of potentially differentially expressed UGS cDNA clones.

[0051] In particular, Table 1 presents the results of the library analysis of 787 cDNA UGS-derived ESTs using the Swissprot database. TABLE 1 Results of the library analysis of 787 cDNA UGS-derived ESTs using the Swissprot database SWISSPROT release 35 plus updates with fasty3_t -H -n -w 80 -m 6 KTUP = 2; Unknown Eθ> .1 = 581; Known Eθ< 1.00E-6 = 168; Uncertains(inbetween) = 66 Clone Prot. Locus Acc. # Identity GTPases ua1e1f RB5C_CANFA P51147 canis familiaris (dog). ras-related protein Rab-5c. 10/ (216 aa) 8.4e-32 ua1h3f KPCI_HUMAN P41743 homo sapiens (human). protein kinase c, iota type (ec 2 (587 aa) 1e-51 ug114rcon RB5B_HUMAN P35239 homo sapiens (human), and mus musculus(mouse). ras-related protein Rab-5b (215 aa) 2.4e-29 ug126 CYA6_MOUSE Q01341 mus musculus (mouse). adenylate cyclase, type vi (ec 4. (1165 aa) 1.7e-24 ug139 MMR1_MOUSE P36916 mus musculus (mouse). possible gtp-binding protein mmr1 (430 aa) 2.6e-26 *ug307cons GBLP_HUMAN P25388 homo sapiens (human), mus musculus (mouse) guanine nucleotide binding protein (317 aa) 3.2e-57 *ug308t GBLP_HUMAN P25388 homo sapiens (human), mus musculus (mouse) guanine nucleotide binding protein (317 aa) 8.8e-55 ug326 GBLP_HUMAN P25388 homo sapiens (human), mus musculus (mouse) guanine nucleotide binding protein (317 aa) 4.6e-61 Protein Kinases ug014rcon 143E_ARATH P48347 arabidopsis thaliana (mouse-ear cress). 14-3-3-like protein GF14 epsilon (254 aa) 1e-19 ug265 KPCE_MOUSE P16054 mus musculus (mouse). protein kinase c, epsilon type (NPKC-epsilon) (737 aa) 3e-28 ug365 143Z_RAT P35215 rattus norvegicus (rat), and mus musculus 14-3-3 protein (245 aa) 5.8e-50 ug425 143T_HUMAN P27348 homo sapiens (human). 14-3-3 protein tau (14-3-3 protein theta)(14-3-3 protein T-cell) (245 aa) 6.5e-18 ug479 P153_HUMAN P53807 h phosphatidylinositol-4-phosphate 5-kinase type iii (e(406 aa) 3.3e-18 ugs020 KC21_RAT P19139 rattus norvegicus (rat). casein kinase ii, alpha chain (c(391 aa) 2.2e-20 *ugs186oft CLK1_MOUSE P22518 mus musculus (mouse). protein kinase clk (ec 2.7.1.-) ( (483 aa) 5.7e-08 (see also clk-4 AF033566 (1549 nt) 9.7e-39) Structural Proteins ug023rcon AP19_MOUSE Q00382 m clathrin coat assembly protein ap19 (clathrin coat assembly protein) (158 aa) 2.5e-27 ug049rcon YAD5_YEAST P39730 saccharomyces cerevisiae (baker's yeast). 112.3 kd protein PYK1-SNC21 intergenic region (1002 aa) synaptobrevins homolog. Vesicle fusion and exocytosis regulator. 2.7e-29 ug052rcon CA13_RAT P13941 rattus norvegicus (rat). collagen alpha 1(iii) chain (fra (636 aa) 1.4e-24 ug061rcon CALX_MOUSE P35564 mus musculus (mouse). calnexin precursor. 11/1995 (591 aa) 1.6e-27 ug0982rcon CA13_RAT P13941 rattus norvegicus (rat). collagen alpha 1(iii) chain (fra (636 aa) 1.2e-48 ug116rcon DREB_CHICK P18302 gallus gallus (chicken). drebrins e1 and e2. 6/1994 (607 aa) 3.7e-07 ug136rcon KELC_DROME Q04652 drosophila melanogaster (fruit fly). ring canal protein (intercell comm.: cytoplasm exchge regulator) (689 aa) 3e-10 ug164rcon VIME_MOUSE P20152 mus musculus (mouse). vimentin. 10/1996 (465 aa) 2.9e-29 ug170rcon FBLC_MOUSE Q08878 mus musculus (mouse). fibulin-1, isoform c precursor (b (685 aa) 8.4e-51 ug256 ANX5_MOUSE P48036 m annexin v (lipocortin v) (endonexin ii) calphobindin (319 aa) 1.7e-19 ug284 ANKC_HUMAN Q01485 homo sapiens (human). ankyrin, brain variant 2 (ankyrin (1839 aa) 2.7e-08 ug297 ATCP_RAT P11505 rattus norvegicus (rat). calcium-transporting atpase plasma membrane calcium pump (1176 aa) 6.5e-40 ug382 CTNA_MOUSE P26231 mus musculus (mouse). alpha-catenin (102 kd cadherin-as (906 aa) 6.4e-31 ug401 VP36_CANFA P49256 canis familiaris (dog). vesicular integral-membrane pro (356 aa) 6.5e-43 ug415 MYST_HUMAN P35749 homo sapiens (human). myosin heavy chain, smooth muscle (1086 aa) 2.9e-12 ug416 SPCB_HUMAN P11277 homo sapiens (human). spectrin beta chain, erythrocyte. (2137 aa) 5.2e-34 ug427 TBB1_PEA P29500 pisum sativum (garden pea). tubulin beta-1 chain. 10/1994 (450 aa) 2.8e-14 ug420 NFM_MOUSE P08553 mus musculus (mouse). neurofilament triplet m protein (1 (848 aa) 1.9e-30 ug481 NFM_MOUSE P08553 mus musculus (mouse). neurofilament triplet m protein (1 (848 an) 1.9e-30 ug517 GPCK_MOUSE P51655. mus musculus (mouse). k-glypican precursor 10/1996 (557 aa) 1.6e-43 ug523 KIF1_MOUSE P33173 mus musculus (mouse). kinesin-like protein kif1 (fragme (147 aa) 1.5e-25 ugs016 PGS2_MOUSE P28654 mus musculus (mouse). bone proteoglycan ii precursor (p (354 aa) 5e-22 ugs072 TPM_RAT P18344 rattus norvegicus (rat). tropomyosin alpha chain, brain- 3 (245 aa) 2.3e-23 ugs117 TALI_MOUSE P26039 mus musculus (mouse). talin. 2/1994 (2541 aa) 7.1e-20 ugs160 EG5_HUMAN P52732 homo sapiens (human). kinesin-like protein eg5. 10/1996 (1057 aa) 1.2e-17 Growth Factors, Cytokines & Binding Proteins ua2h6f IBP2_MOUSE P47877 mus musculus (mouse). insulin-like growth factor bindin (305 aa) 2.5e-35 ug130 1BP5_MOUSE Q07079 inns musculus (mouse). insulin-like growth factor bindin (271 aa) 3.8e-50 ug150rcon TYB4_MOUSE P20065 mus musculus (mouse). thymosin beta-4. 5/1992 (50 aa) 2.1e-14 ug264 AAAT_MOUSE P51912 mus musculus (mouse). insulin-activated amino acid tran (553 aa) 9.7e-33 ug300 PC4_MOUSE P19182 mus musculus (mouse). interferon-related protein pc4 (tp (449 aa) 1.2e-24 ug324 ENDR_BOVIN P07106 bos taurus (bovine). endozepine-related protein precurs (533 aa) 3.9e-16 ug347 MFGM_MOUSE P21956 mus musculus (mouse). milk fat globule-egf factor 8 pre (463 aa) 4e-10 ug394 THA1_MOUSE P16416 mus musculus (mouse). thyroid hormone receptor alpha-1. (410 aa) 6.7e-25 Ribosomal Proteins and Translation ug029rcon RL11_RAT P25121 rattus norvegicus (rat). 60s ribosomal protein 111. 6/199 (177 aa) 6.4e-38 ug068rcon RL13_MOUSE P47963 mus musculus (mouse). 60s ribosomal protein 113 (a52). (212 aa) 2.1e-37 ug086rcon KS61_MOUSE P18653 mus musculus (mouse). ribosomal protein s6 kinase ii al (724 aa) 3.6e-10 ug129 RL2A_RAT P18445 rattus norvegicus (rat). 60s ribosomal protein 127a. 11/1 (147aa) 1.4e-46 ug127 SR72_CANFA P33731 canis familiaris (dog). signal recognition particle 72 (670 aa) 7.4e-48 ug149rcon RL5_RAT P09895 rattus norvegicus (rat). 60s ribosomal protein 15. 10/1996 (296 aa) 1.4e-52 ug172rcon RL13_MOUSE P47963 mus musculus (mouse). 60s ribosomal protein 113 (a52). (212 aa) 5.7e-44 ug187rcon RL2A_MOUSE P14115 mus musculus (mouse). 60s ribosomal protein 127a (129). (147 aa) 1.3e-44 ug194 RL31_HUMAN P12947 homo sapiens (human), and rattus norvegicus (rat). 60s (125 aa) 1.5e-36 ug290 RS15_HUMAN P11174 homo sapiens (human), mus musculus (mouse), rattus norv ribosomal protein S15 (144 aa) 4e-43 ug303 EF1A_MOUSE P10126 mus musculus (mouse). elongation factor 1-alpha(ef-1- a (462 aa) 6.1e-62 *ug334 SR14_MOUSE P16254 mus musculus (mouse). signal recognition particle 14 kd (110 aa) 6.9e-42 *ug354cons RLA2_HUMAN P05387 homo sapiens (human). 60s acidic ribosomal protein p2. (115 aa) 1.2e-29 ug381 EF1B_HUMAN P24534 homo sapiens (human). elongation factor 1-beta (ef-1- beta) (224 aa) 8.8e-39 ug460 RS23_HUMAN P39028 homo sapiens (human), and rattus norvegicus (rat). 40s ribosomal protein S23 (143 aa) 1.9e-11 ug475 RS24_HUMAN P16632 homo sapiens (human), rattus norvegicus (rat), mus musc 40s ribosomal protein S24 (S19) (133 aa) le-33 ug502 SYHH_HUMAN P49590 homo sapiens (human). histidyl-trna synthetase homolog (506 aa) 5.5e-13 ugs059 RS6_HUMAN P10660 homo sapiens (human), rattus norvegicus (rat), and mus m ribosomal protein S6 (phosphoprotein NP33)(249 aa) 1.1e-24 ugs095 RSP4_HUMAN P08865 homo sapiens (human). 40s ribosomal protein sa (p40) (34/67 kDa laminin binding protein) (295 aa) 6.3e-23 ugs114 S61A_RAT P38378 rattus norvegicus (rat). protein transport protein sec61p (ribosomal associated transport protein) (475 aa) 2.1e-23 ugs142 RL7A_MOUSE P12970 mus musculus (mouse). 60s ribosomal protein 17a (surfeit locus protein 3) (265 aa) 1.4e-13 ugs188 RS18_HUMAN P25232 homo sapiens (human), rattus norvegicus (rat), and mus musculus 40S ribosomal protein S18 (KE3) (152 aa) 1.3e-21 ugs226 RS24_XENLA P02377 xenopus laevis (african clawed frog). 40s ribosomal protein S24 (S19) (132 aa) 4e-27 Transcription Factors ua1a2 SON_HUMANP 18583 homo sapiens (human) son protein (son3). DNA binding protein w/mos and myc homology 11/1995 (1523 aa) 2.3e-13 ug027rcon PUR_MOUSE P42669 mus musculus (mouse). transcriptional activator protein (321 aa) 3.1e-07 ug087rcon TYY1_MOUSE Q00899 mus musculus (mouse). transcriptional repressor protein (414 aa) 2.5e-33 ug113rcn1o POL2_MOUSE P11369 mus musculus (mouse). retrovirus-related pol polyprotei (1300 aa) 8.8e-36 ug228 ZN83_HUMAN P51522 homo sapiens (human). zinc finger protein 83 (zinc fing (428 aa) 6.4e-08 ug243 POL2_MOUSE P11369 mus musculus (mouse). retrovirus-related pol polyprotei (1300 aa) 7.1e-51 ug249 POL2_MOUSE P11369 mus musculus (mouse). retrovirus-related pol polyprotei (1300 aa) 1.8e-10 ug271 CABA_MOUSE Q99020 mus musculus (mouse). carg-binding factor-a (cbf-a). 11 (285 aa) 3.5e-08 *ug277t HXAD_AMBME P50210 ambystoma mexicanum (axolotl). homeotic protein hox-a13 (107 aa) 1.2e-34 (other locus 1399859 Acc #U59322) ug289 SN21_HUMAN P28370 homo sapiens (human). possible global transcription activator (976 aa) 1.9e-09 ug313 POL2_MOUSE P11369 mus musculus (mouse). retrovirus-related pol polyprotei (1300 aa) 2.7e-37 ug367 ETF_MOUSE P48301 mus musculus (mouse). embryonic tea domain- containing factor (445 aa) 2.1e-23 ug486 CL36_RAT P52944 rattus norvegicus (rat). lim protein clp36. (contains homeodomain of lin-11) 10/1996 (327 aa) 2e-24 ugs101 POL2_MOUSE P11369 mus musculus (mouse). retrovirus-related pol polyprotei (1300 aa) 5.8e-23 Mitochondrial ug002rcon ATP6_MOUSE P00848 mus musculus (mouse). atp synthase a chain (ec 3.6.1.34 (226 aa) 1.2e-52 ug007rcon CYB_MOUSE P00158 mus musculus (mouse). cytochrome b (ec 1.10.2.2). 3/1992 (381 aa) .8e-85 ug045con NU5M_MOUSE P03921 mus musculus (mouse). nadh-ubiquinone oxidoreductase ch (607 aa) 5.1e-37 ug063rcon GR75_MOUSE P38647 mus musculus (mouse). mitochondrial stress-70 protein p (679 aa) 3.4e-31 ug103rcon ATP6_MOUSE P00848 mus musculus (mouse). atp synthase a chain (ec 3.6.1.34 (226 aa) 1.1e-19 ug207 NU5M_MOUSE P03921 mus musculus (mouse). nadh-ubiquinone oxidoreductase ch (607 aa) 8.9e-55 ug296 ATP6_MOUSE P00848 mus musculus (mouse). atp synthase a chain (ec 3.6.1.34 (226 aa) 7e-27 ug336 ATP6_MOUSE P00848 mus musculus (mouse). atp synthase a chain (ec 3.6.1.34 (226 aa) 7.2e-32 ug363 NU4M_MOUSE P03911 mus musculus (mouse). nadh-ubiquinone oxidoreductase ch (459 aa) 1.4e-46 ug378 ATPQ_RAT P31399 rattus norvegicus (rat). atp synthase d chain, mitochondr (160 aa) 2.8e-11 ug489 NU1M_MOUSE P03888 mus musculus (mouse). nadh-ubiquinone oxidoreductase ch (315 aa) 4.5e-61 ug510 COX3_MOUSE P00416 mus musculus (mouse). cytochrome c oxidase polypeptide (261 aa) 2.8e-11 ugs064 ATP6_MOUSE P00848 mus musculus (mouse). atp synthase a chain (ec 3.6.1.34 (226 aa) 1.1e-18 ugs091 NU6M_MOUSE P03925 mus musculus (mouse). nadh-ubiquinone oxidoreductase ch (172 aa) 3.7e-32 ugs094 ATP6_MOUSE P00848 mus musculus (mouse). atp synthase a chain (ec 3.6.1.34 (226 aa) 4.6e-19 RNA Splicing, Binding, RNPs, etc... ug072rcon P68_HUMAN P17844 homo sapiens (human). p68 protein (rna helicase). 6/1994 (614 aa) 1.2e-16 ug145 HMT1_YEAST P38074 saccharomyces cerevisiae (baker's yeast). hnrnp arginine n-methyltransferase (348 aa) 4.2e-17 ug225 ROA1_MOUSE P49312 mus musculus (mouse). heterogeneous nuclear ribonucleop (319 aa) 3.7e-15 ug293 PSF_HUMAN P23246 homo sapiens (human). ptb-associated splicing factor (ps (707 aa) 1.3e-41 ug310 FUS_HUMAN P35637 homo sapiens (human). ma-binding protein fus/tls. 11/19 (526 aa) 1.7e-27 *ug311icons PSF_HUMAN P23246 homo sapiens (human). ptb-associated splicing factor (ps (707 aa) 1.7e-25 ug391 RSMB_MOUSE P27048 mus musculus (mouse). small nuclear ribonucleoprotein a (231 aa) 2.6e-25 *ug485ors RNPL_HUMAN P98179 homo sapiens (human). putative rna-binding protein rnpl (157aa) 3.1e-12 ugs115 UBIQ_HUMAN P02248 homo sapiens (human), bos taurus (bovine), UBIQUITIN (76 aa) 3e-14 ugs128 P68_HUMAN P17844 homo sapiens (human). p68 protein (rna helicase). 6/1994 (614 aa) 2.6e-13 Peptidases, Proteinases, Isomerases, Transferases *ug101rcon DPP4_MOUSE P28843 mus musculus (mouse). dipeptidyl peptidase iv (ec 3.4.1) (760 aa) 5.7e-07 ug153rcon PPI1_MOUSE P53810 mus musculus (mouse). phosphatidylinositol (ptdins) transfer protein alpha (270 aa) 9.2e-26 ug188rcon NMT_HUMAN P30419 homo sapiens (human). glycylpeptide n- tetradecanoyltransferase (peptide N- myristoyltransferase) (NMT) (416 aa) 1e-51 ug211 COGT_MOUSE P53690 mus musculus (mouse). matrix metalloproteinase-14 precu (582 aa) 3.2e-51 *ug335 NEP_RAT P07861 rattus norvegicus (rat). neprilysin (ec 3.4.24.11) (neutral endopeptidase) (749 aa) 5e-20 ug458 VKGC_HUMAN P38435 homo sapiens (human). vitamin k-dependent gamma glutamyl-carboxylase (758 aa) 1.7e-34 ugs030 PUR6_RAT P51583 r multifunctional protein ade2 (amidophosphoribosyltransferase) Cell cycle dependent regulation (425 aa) 1.7e-16 ugs123 PDI_MOUSE P09103 m protein disulfide isomerase precursor (pdi). (509 aa) 5e-11 ugs180 AMP2_RAT P38062 rattus norvegicus (rat). methionine aminopeptidase 2 (478 aa) 7.2e-27 ugs190 FUCO_HUMAN P04066 homo sapiens (human). tissue alpha-1-fucosidase precurs (Lysosomal storage) (461 aa) 6.8e-25 Chromosomal Associated ug040rcon RCC_MESAU P23800 mesocricetus auratus (golden hamster). regulator of chromosomal condensation (421 aa) 4.8e-07 ugs010 H33_HUMAN P06351 homo sapiens (human), mus musculus (mouse), rattus norve histone H3.3 (H3b) (135 aa) 4.3e-18 ugs146 TPR_HUMAN P12270 homo sapiens (human). nucleoprotein tpr. 10/1996 (2349 aa) 1.9e-15 Heat Shock, Chaperones, Stress-Induced ugo42con HS9B_MOUSE P11499 mus musculus (mouse). heat shock protein hsp 84 (tumor specific transplantation antigen) (723 aa) 3.7e- 51 ug356 HS7C_RAT P08109 rattus norvegicus (rat), and mus musculus (mouse). heat shock cognate 71kDa (646 aa) 6.3e-58 Neural Specific ug379 HIPP_HUMAN P41211 homo sapiens (human). neuron specific calcium- binding protein hippocalcin (BDR-2) (192 aa) 9.2e-09 ugs023 NED4_MOUSE P46935 mus musculus (mouse). nedd-4 protein (ec6.3.2.-) neural precursor cell protein (frag (957 aa) 4.8e-19 Hypothetical *ug093rcon YO11_MOUSE P11260 mus musculus (mouse). hypothetical protein orf-1137. (L1Md domain protein, repetitive element retroposon- like) 7/ (379 aa) 4.4e-46 ug095rcon YJZ4_YEAST P47095 saccharomyces cerevisiae (baker's yeast). hypothetical (244 aa) 8.3e-18 ug309 YNK7_YEAST P53930 saccharomyces cerevisiae (baker's yeast). hypothetical (226 aa) 2.5e-14 ug412 YCFB_HAEIN P44551 haemophilus influenzae. hypothetical protein hi0 174. 10 (418 aa) 6.5e-11 Nucleotide metabolism (Cytosolic) ug084rcon THIO_MOUSE P10639 inns musculus (mouse). thioredoxin (atl-derived factor) ribont-deoxyribont converter and general reducer (104 aa) 2.3e-21 ug413 ARF5_HUMAN P26437 homo sapiens (human), and rattus norvegicus (rat). adp-ribosylation factor 5 (179 aa) 5.2e-07 Unknown ug480 IGEB_MOUSE P03975 mus musculus (mouse). IgE-binding protein. 4/1988 (557 aa) 1.5e-24 ugs044 TLM_MOUSE P17408 mus musculus (mouse). tlm protein (tlm oncogene). 12/199 (317aa)1.4e-07 Vector Associated (Tet-R/Beta-gal) ug016_38_80 TER1_ECOLI P03038 escherichia coli , tetracycline repressor protein class (216 aa) 4.7e-27 ug060 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 1.8e-32 ug100rcon TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 9.5e-08 ug108rcon TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 4.6e-31 ug122rcon TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 1.7e-30 ug165rcon BGAL_ECOLI P00722 escherichia coli. beta-galactosidase (ec 3.2.1.23) (lac (1023 aa) 3.1e-13 ug166rcon TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 5.2e-35 ug193 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 2.2e-24 ug199 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa)3.5e-11 ug204 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 2.2e-23 ug215 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 1.5e-12 ug231 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 4.8e-27 ug235 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 1e-29 ug236 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) ug268 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 4.5e-16 ug283 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 1.1e-31 *ug316cons TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 3e-20 ug327 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 5.1e-34 ug349 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 8.7e-31 ug362 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 2.3e-32 ug375 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 as) 9e-29 ug386 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 2.3e-23 ug389 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 3e-27 ugs015 TER1_ECOLI P03038 escherichia coli. tetracycline repressor protein class (216 aa) 7.6e-13

[0052] Table 2 presents the results of the library analysis of 787 cDNA UGS-derived ESTs using the GENPEPT translated protein database (rel 102.0). TABLE 2 Results of the library analysis of 787 cDNA UGS-derived ESTs using the GENPEPT translated protein database (rel 102.0) GENPEPT Translated Protein Database (rel 102.0); Additional known Eθ < 1.00E-6 = 28 Clone Prot. Locus Acc. # Identity Protein Kinases ug135 1008544 U35113 breast adenocarcinoma metastasis- associated gene (contains SH3 domains) Homo sapiens (715aa) 2.5e-14 Membrane/Structural Proteins ug001rcon 1228041 D83779 contains 9 hydrophobic domains. ÄHomo sapiens AÅ(1356 aa) 3.8e-66 the KIAA0195 gene is expressed ubiquitously.; the KIAA0195 protein retains 9 hydrophobic domains. ug202 1109847 U41538 R04E5.6 gene product Caenorhabditis elegans (430 aa) 3.3e-14 Similar to cytoplasmic intermeidate filament protein” (chromosome III) ug244 165704 M76233 Rabbit smooth muscle myosin light chain kinase mRNA, comp (1147 aa) 2.1e-18 ugs112 1707522 Y08612 88kDa nuclear pore complex protein ÄHomo (741 aa) 2.6e-22 Growth Factors, Cytokines & Binding Proteins ug094rcon 1480110 X99643 HP1-BP38 protein Mus musculus (TIF-like molecule) nuclear receptor ligand binding domain interactive protein (852 aa) 2.2e-40 Transcription Factors ug279 1203987 L40331 homologuous to yeast silent information regulatory 2 prot (381 aa) 6.1e-07 ug374 431953 X76302 nucleic acid binding protein Homo sapiens (163 aa) 1.1e-08 Mitochondrial ug449 1332384 M27315 Rattus norvegicus cytochrome c oxidase subunit I Rattus (514 aa) 2.5e-38 RNA Splicing, Binding, RNPs, etc... ug019rcon 1374782 D85414 possible ubiquitin protein ligase Mus musculus (957 aa) 5.5e-05 ug168rcon 619302 S72641 RNA-binding protein = Merc äalternatively spliced (296 aa) 1.1e-28 ug081rcon 603949 D43947 KIAA0100 is a human counterpart of mouse el gene. Homo sa (2092 aa) 3.5e-55 Peptidases, Proteinases, Isomerases, Transferases ualc6f 1483249 Z78012 C52E4.6 Caenorhabditiselegans 23S protase regulatory subnunit 7” “Alpha 1,2 mannosidase”, “Cathepsin-like cysteine protease”, “Guanylate cyclase”,“HTG”, “Low- density lipoprotein receptor”, “Macrophage migration inhibitory factor like”, “Small nuclear ribonucleoprotein”}(534 aa) 2.4e-29 ug062rcon 1240019 Z70287 R09E10.7 Caenorhabditis elegans 2.2mb of chromoIII “HTG”, “Long- chain-fatty-acid-CoA ligase”, “Protein-tyrosine phosphatase”} (1791 aa) 4.4e-13 ug312 802105 S74907 PP1M M110 = protein phosphatase 1M 110 kda regula (976 aa) 1.5e-27 ug329 1808596 Y08826 alkyl-dihydroxyacetonephosphate synthase (658 aa) 4e-38 Heat Shock, Chaperones, Stress-Induced ug111nov 687844 U21320 contains TPR domain-like repeats Caenorhabditis elegans (molecular chaperone for HSP's and other) (1194 aa) 6.7e-32 ug167rcon 860712 U28735 coded for by C. elegans cDNA cm06e4; coded for by C. elegans (1493 aa) 9.9e-14 similar to killer toxin-resistance protein 5 (SP: KRE5_YEAST, P22023) Unknown ug337 1469878 D63482 The KIAA0148 gene product is related to KIAA0041 and KIAA (471 aa) 6.4e-13 (ESTs of human cell line KG-1) *ug371f 1480863 U63332 super cysteine rich protein; SCRP Homo sapiens (46 aa) 1.9e-08 (expression appears ubiquitois) ugs137 532816 U13876 similar to S. cerevisiae hypothetical 240.3 kd protein in C. Elegans similar to MSH3 3′sequence. (2500 aa) 2.4e-08 ugs153 1483249 Z78012 C52E4.6 Caenorhabditis elegans (Chromosome 5) (534 aa) 5.1e-11 ugs206 291844 L13434 Human chromosome 3p21.1 gene sequence from lung cancer, complete cds., conceptual translation (256 aa) 1.2e-18 Tet-Repressor/βeta-gal Cloning Vector ug067rcon 1132426 U39779 beta-galactosidase alpha polypeptide ÄCloning vector pTri (139 aa) 2.9e-10 ug258 1132426 U39779 beta-galactosidase alpha polypeptide ÄCloning vector pTri (139 aa) 6.7e-09

[0053] Table 3 presents the results of the library analysis of 787 cDNA UGS-derived ESTs using the primate rodent GB103 database. TABLE 3 Results of the library analysis of 787 cDNA UGS-derived ESTs using the primate rodent GB103 database GenBank GB103: Primate and Rodent divisions fasta3_t -H -n -w 80 -m 6 -1 /seqlib/lib/fastlibs % PR 6 Clone Locus Acc. # Identity GTPases ug270 AF070603 Homo sapiens clone 24584 beta-subunit signal transducing proteins (Gs/Gi) (1889 nt) 4.6e-55 ug447 HSEWSGAR Y07848 Homo sapiens EWS, gar22, rrp22 and bam22 genes. 5/98 (79468 nt) 7.3e-65 (Identification of new members of the Gas2 and Ras families in the 22q12 chromosome region.) ug451 HSRANBP5 Y08890 H.sapiens mRNA for Ran_GTP binding protein 5. 9/97 (4826 nt) 9.3e-100 ugs149 HSU50078 U50078 Human guanine nucleotide exchange factor p532 mRNA, complet (15171 nt) 1.6e-36 ugs177 HSU90268 U90268 Human Krit1 mRNA, complete cds. 6/97 (2004 nt) 2.6e-19 Protein Kinases ug195 MUSPGK1PS2 M23962 Mus musculus phosphoglycerate kinase (Pgk1- ps2) processed (1753 nt) 3.1e-68 *ug441ors AF027504 Mus musculus putative membrane-associated guanylate kinase 1 (Mag (919 nt) 1.8e-2 ugs110 HSDAPK X76104 H.sapiens DAP-kinase mRNA. 4/97 (5910 nt) 7e- 11 (Very repetitive jdr) ugs147 MMU51866 U51866 Mus musculus casein kinase II alpha subunit mRNA, complete (1552 nt) 1.4e-49 Structural Proteins/ECM *ug102cons RATCTTG M80829 Rat troponin T cardiac isoform gene, complete cds. 9/96 (19185 nt) 2.3e-12 (Highly repetitive jdr) ug174rcon MMU48797 U48797 Mus musculus astrotactin mRNA, complete cds. 5/96 (6863 nt) 1.4e-31 ug206 RATSTPBCB D83349 Rat mRNA for short type PB-cadherin, complete cds. 7/96 (4153 nt) 3.9e-16 ug392 AF078705 Mus musculus vascular adhesion protein-1 gene, complete cds. 9/98 (14357 nt) 2.7e-19 ug465 MUSCK15 D16313 Mouse cytokeratin 15 gene, complete cds. 3/96 (6149 nt) 4.7e-58 ug498 RRU04320 U04320 Rattus norvegicus Wistar alpha B-crystallin gene, complete (6806 nt) 6e-14 ug521 RATCRYG M19359 Rat gamma-crystallin gene cluster, encoding gamma-A (gamma 1 (54670 nt) 1.6e-09 ug525 MMSPARCR X04017 Mouse mRNA for cysteine-rich glycoprotein SPARC. 9/93 (2079 nt) 1.1e-131 ugs217 MUSCOL4A J04448 Mouse alpha 1 and 2 collagen type IV genes, 5′ end. 1/94 (1200 nt) 9.6e-29 Oncogenes/Tumor Suppressors/Apoptosis ug142 AF060868 Mus musculus tumor susceptibility protein 101 (tsg101) gene, comp (33613 nt) 2.6e-12 ug219 AF060868 Mus musculus tumor susceptibility protein 101 (tsg101) gene gene, comp (33613 nt) 2.5e-13 ug201 MUSRRG D10837 Mus muculus rrg (ras recision gene) mRNA, tumor suppressor opposes ras action, partial sequence. 1 (1942 nt) 2.6e-104 ug218 HSU41635 U41635 Human 0S-9 precursor mRNA, complete cds. 8/96 (sarcoma associated gene) (2736 nt) 6.5e-34 *ug503s AF017989 Mus musculus secreted apoptosis related protein 1(Sarp1) mRNA, c (2031 nt) 8.3e-57 ugs216 MMU50850 U50850 Mus musculus retinoblastoma-related protein p130 mRNA, (4013 nt) 2e-48 Growth Factors, Cytokines & Binding Proteins ug140 MMIGFIIE6 X71922 M.musculus gene for IGF-II, exon 6. 7/95 (3321 nt) 1.2e-116 ug306 MMTNFBG Y00137 Mouse tumor necrosis factor-beta (lymphotoxin) gene. 5/93 (3219 nt) 6.4e-13 (polyA jdr) ug414 MMIL5G X06271 Murine gene for interleukin 5 (eosinophil differentiation fac (6727 nt) 1.5e-12 ug518 AF063020 Homo sapiens lens epithelium-derived growth factor mRNA, complete (3377 nt) 2.7e-57 ug522 MMU06950 U06950 Mus musculus C57BL/6 lymphotoxin-beta, lymphotoxin-alpha, (Murine TNF-alpha?beta locus) (15213 nt) 4e-21 Growth Factor Induced ug077rcon MUSDIP D44443 Mouse mRNA for dexamethasone induced apoptosis in T-cells, complete cds. 5 (573 nt) 1.3e-08 ug111rcon MUSDIP D44443 Mouse mRNA for dexamethasone induced apoptosis in T-cells, complete cds. 5 (573 nt) 1.7e-17 ug124 RNPTHR202 X95079 R.norvegicus mRNA for parathyroid hormone regulated sequen (202 nt) 1.4e-19 ugs167 MUSGRP784 D78645 Mouse mRNA for 78 kDa glucose-regulated protein, complete (2408 nt) 1.5e-27 Ribosomal Proteins and rRNA ug257 MM45SRRNA X82564 M.musculus 45S pre rRNA gene. 4/96 (22118 nt) 1.6e-30 ug325 MM45SRRNA X82564 M.musculus 45S pre rRNA gene. 4/96 (22118 nt) 2.6e-67 ug361 MM45SRRNA X82564 M.musculus 45S pre rRNA gene. 4/96 (22118 nt) 4.1e-42 ug444 MM45SRRNA X82564 M.musculus 45S pre rRNA gene. 4/96 (22118 nt) 4.5e-88 ugs005 MMJ1PRO Y00225 Murine mRNA for J1 protein, yeast ribosomal protein L3 homol (1276 nt) 3.4e-13 ugs038 MM45SRRNA X82564 M.musculus 45S pre rRNA gene. 4/96 (22118 nt) 1.5e-46 Membrane Proteins/Receptors ug083rcon MMHC135G15 AF050157 Mus musculus major histocompatibility locus class II re (79435 nt) 5.2e-10 ug093f MMAE000663 AE000663 Mus musculus TCR beta locus from bases 1 to 250611 (sec (79890 nt) 4.7e-11 (TCR = T cell receptor jdr) ug119 MMAE000664 AE000664 Mus musculus TCR beta locus from bases 250554 to 501917 (79704 nt) 1.8e-18 ug131 MMAE000665 AE000665 Mus musculus TCR beta locus from bases 501860 to 700960 (40877 nt) 1.8e-13 ug133 AF100956 Mus musculus major histocompatibility locus class II region; Fas- (79588 nt) 3e-06 ug155rcon MMHC438N12 AF049850 Mus musculus major histocompatibility locus class III r (70941 nt) 3.3e-19 ug176rcon MUSSVA L44117 Mus musculus (clone GSmSVA) seminal vesicle autoantigen gene, (5307 nt) 1.2e-09 (Highly repetitve, but it is in the right place jdr) ug200 MMHC135G15 AF050157 Mus musculus major histocompatibility locus class II re (79513 nt) 5.2e-13 ug214 MMU97066 U97066 Mus musculus sulfonylurea receptor 2B (SUR2) mRNA, Protein associated with potassium ATPase transporter (6081 nt) 5.5e-07 ug222 MUSLYT3A6 M22070 Mouse MHC class I T-cell surface antigen gene Lyt-3-a enco (1249 nt) 2.8e-15 *ug254 MUSBA D82019 Mouse gene for basigin, complete cds (exon 1-7). 2/97 (11763 nt) (Basigin, a new, broadly distributed member of the immunoglobulin superfamily, has strong homology with both the immunoglobulin V domain and the beta-chain of major histocompatibility complex class II antigen.)1/98 (1302 nt) 5.9e-95 ug260 AF018261 Rattus norvegicus EH domain binding protein Epsin mRNA, complete (calthrin mediated endocytosis) (2047 nt) 4.3e-11 ug287 MMZNT4S3 AF004099 Mus musculus zinc transporter (ZnT4) gene, fragment 3, important for Zn uptake and sequestration into endosome/lysosomal and synaptic vesicles (1371 nt) 1.1e-08 ug369 AF007558 Mus musculus hemocbromatosis (HFE) gene (Critical molecule involved in cellular iron homeosatsis. Related to MHC genes., complete cds. 2/98 (14000 nt) 1.5e-12 ug376 MUSBB2R L27595 Mus muscaris bradykinin B2 receptor (B2R) gene, complete cds (8934 nt) 1.5e-07 ug454 MMCLCNVI4 AF030104 Mus musculus putative chloride channel protein CLC6 (Clc (14925 nt) 7e-11 ug459 MMHC135G15 AF050157 Mus musculus major histocompatibility locus class II re (8222 nt) 2.7e-09 ug462 HUMZD39G09 AF086249 Homo sapiens full length insert cDNA clone ZD39G09. 8/9 (555 nt) 7.8e-12 Similar to INTEGRIN BETA-1 ug468 MMMMH461 AF027865 Mus musculus Major Histocompatibility Locus class II regi (79560 nt) 7.6e-110 ug474 AF100956 Mus musculus major histocompatibility locus class II region; Fas- (79856 nt) 3.7e-15 *ug493ors MMEZR X60671 M.musculus mRNA for ezrin. 8/96 (2701 nt) (A gene family consisting of ezrin, radixin and moesin. Its specific localization at actin filament/plasma membrane association sites.) 2.9e- 57 ugs024 MMMHC29N7 AF030001 Mus musculus major histocompatibility locus class III re (79848 nt) 2.9e-05 ugs126 AB008110 Rattus norvegicus RTI-DOb gene, partial cds. 7/98 (8818 nt) 4.6e-13 (major histocompatibility gene) ugs133 MUSTCRA M64239 Mouse T-cell receptor alpha/delta chain locus. 8/92 (79772 nt) 1.8e-10 Transcription Factors/Co-factor ug141rcon HSU10323 U10323 Human nuclear factor NF45 mRNA, complete cds. 8/94 (1552 nt) 1.9e-32 ug156rcon AF075587 Homo sapiens protein associated with Myc mRNA, complete cds. 8/98 (14807 nt) 8.1e-94 ug157rcon AF010403 Homo sapiens ALR mRNA, complete cds. 9/97 (trx-G paralogue, trithorax gene complex, homeotic) (15789 nt) 8.2e-21 ug159 MMU92454 U92454 Mus musculus WW domain binding protein 5 mRNA, partial cds. (proline-rich, sh3 domain interactive protein) involved in regulation of transcription in development of kidney and limbs. Homologue of Drosophila enabled. (647 nt) ug192rcon HSU05040 U05040 Human FUSE binding protein mRNA, complete cds. 5/94 (2325 nt) 1.4e-69 (The far upstream element-binding proteins comprise an ancient family of single-strand DNA-binding transactivators; myc gene transcriptional controller) ug224 RRU17837 U17837 Rattus sp. zinc finger protein RIZ mRNA, complete cds. 8/95 (6152 nt) 8.7e-29 ug278 MMU48363 U48363 Mus musculus transcriptional activator alpha- NAC (nascent polypeptide-associated complex) gene (12989 nt) 2.3e-20 ug371cons MMHOXD11 X71422 M.musculus Hoxd-11 gene. 8/93 (5593 nt) 1.4e- 63 ug408 MMU70139 U70139 Mus musculus putative CCR4 protein mRNA, partial cds. 7/97 (9737 nt) 1.3e-07 Characterization of two age-induced intracisternal A-particle-related transcripts in the mouse liver. Transcriptional read-through into an open reading frame with similarities to the yeast ccr4 transcription factor. ug422 AF098161 Mus musculus timeless homolog mRNA, complete cds. 11/98 (4438 nt) 7.1e-47 (Mammalian Circadian Autoregulatory Loop: A Timeless Ortholog and mPER1 Interact and Negatively Regulate CLOCK-BMAL1-Induced Transcription) ug442 AF017085 Mus musculus BAP-135 homolog (general transcription factor II-1: Gtf2i: Diws1t) mRNA, complete cds. 3/98 (4091 nt) 2.9e-104 ug509 AF059275 Mus musculus heat shock transcription factor 1 (Hsfl) gene, parti (11395 nt) 4.9e-19 ugs045 AF056002 Rattus norvegicus Smad4 protein (Smad4) mRNA, complete cds. 4/98 (3041 nt) 1.5e-36 ugs055 RNCEBPRNA X64403 R.norvegicus c/ebp (CC-AAT/enhancer binding protein) gamma mRNA. 6/93 (1430 nt) 1.3e-14 ugs107 HUMYZ84E01 AF086085 Homo sapiens full length insert cDNA clone YZ84E01. 8/9 (650 nt) 2.2e-21 similar to chicken SSDP (sequence-specific single-stranded DNA- binding protein), binds pyrimidine rich regions of DNA ugs192 AF075587 Homo sapiens protein associated with Myc mRNA, complete cds. 8/98 (14807 nt) 8.5e-53 ugs210 RNU09567 U09567 Rattus norvegicus cysteine-rich zinc-finger protein mRNA, widely expressed in fetal brain.. (1403 nt) 9.9e-09 ugs213 MMU41285 U41285 Mus musculus dishevelled-3 (Dvl-3) mRNA, complete cds. 6/96 (2498 nt) 3.8e-13 ugs218 HUMHPLK M55422 Human Krueppel-related zinc finger protein (H- plk) mRNA, com (2873 nt) 1.2e-07 Nuclear/Mitosis Assoc./Chromatin ug232 RATHMG2 D84418 Rat mRNA for chromosomal protein HMG2, complete cds. 4/97 (1072 nt) 6.7e-51 ug246 HSCGGBP AJ000258 Homo sapiens trinucleotide repeat 5-d(CGG)n- double stranded DNA binding protein (779 nt) 4.2e-21 (Fragile X Assoc) ug248 MMHMG17 X12944 Mouse mRNA for HMG-17 chromosomal protein. 9/93 (1113 nt) 1.6e-69 (HMGs are associated with active chromatin jdr) ug281 HSU30872 U30872 Human mitosin mRNA (mitotic progression factor), complete cds. 12/95 (10211 nt) 8.4e-16 ug340 MMU39074 U39074 Mus musculus thymopoietin beta mRNA, complete cds. 5/96 Ubiquitously expressed nuclear proteins. (2170 nt) 6e-74 ug355 HSU70322 U70322 Human transportin (TRN) mRNA, Alternative mechanism to NTS for nuclear translocation. A receptor mediated mechanism via transportin. 10/96 (3054 nt) 2.7e-11 ug453 AF033664 Mus musculus gene-trap line CT 146 cbp 146 (cbp 146) mRNA, Capturing novel mouse genes encoding chromosomal and other nuclear proteins(1032 nt) 6e-100 ug487 MMPSHIS2B X90779 M.musculus psH2B gene. 3/97 (1312 nt) 1.3e-38 (Molecular cloning of mouse somatic and testis- specific H2B histone genes containing a methylated CpG island.) ugs026 MMAJ2636 AJ002636 Mus musculus mRNA for nuclear protein SA2. 11/97 (3871 nt) 4.9e-34 ugs090 AB015330 Homo sapiens mRNA for HRIHFB2007, Selection system for genes encoding nuclear- targeted proteins. 12/98 (865 nt) 1.7e-26 ugs174 AF083322 Homo sapiens centriole associated protein CEP110 mRNA, complete c (3893 nt) 2.3e-29 ugs205 RATSP120 D14048 Rat mRNA for SP120,Nuclear scaffold protein that binds the matrix attachment region DNA 1/95 (3563 nt) 3e-28 ugs227 MMU18295 U18295 Mus musculus histone Hi (0) gene, complete cds. 7/95 (2893 nt) 5.2e-42 (An upstream control region required for inducible transcription of the mouse H1(zero) histone gene during terminal differentiation.) ugs232 HSNUMAMRB Z11584 H.sapiens mRNA for NuMA protein. 4/92 (mitotic spindle associated protein)(7217 nt) 2.8e-32 ugs234 AF022465 Mus musculus high mobility group protein homolog HMG4 (Hmg4) mRNA (1502 nt) 3.9e- 44 (The mouse Hmg4 gene is highly expressed in the embryo; Hmg4 transcripts are barely detectable in adult tissues. The human HMG4 gene, which is extremely similar to its mouse homolog, has been sequenced as part of chromosome X, band q28. HMG4, HMG1, and HMG2 proteins have been highly conserved during vertebrate evolution, suggesting that each has at least some unique property. It is possible that HMG4 is required during development) Mitochondrial ug104rcon RATMT3H3MG M63800 Rattus norvegicus mitochondrial 3-hydroxy-3- methyl glutaryl coenzyme alpha-synthase gene, exon 1 (2074 nt) 4.5e-08 ug180rcon MUSMTHYPB L07096 Mus domesticus strain Mi1P mitocondrion genome, complete s (16303 nt) 1.5e-11 ug181rcon MUSMTHYPA L07095 Mus domesticus strain NZB/B1NJ mitochondrion genome, compl (16303 nt) 5.3e-37 ug205 MUSMTHYPB L07096 Mus domesticus strain Mi1P mitochondrion genome, complete s (16303 nt) 1.1e-51 ug220 MUSMTHYPA L07095 Mus domesticus strain NZB/B1NJ mitochondrion genome, compl (16303 nt) 9.4e-110 ug240 MUSMTHYPA L07095 Mus domesticus strain NZB/B1NJ mitochondrion genome, compl (16303 nt) 6.7e-60 ug411 MUSMTHYPA L07095 Mus domesticus strain NZB/B1NJ mitochondrion genome, compl (16303 nt) 8.2e-87 ug448 MUSMTHYPA L07095 Mus domesticus strain NZB/B1NJ mitochondrion genome, compl (16303 nt) 2.6e-88 ug499 MUSMTCG J01420 Mouse mitochondrion, complete genome. 7/95 (16295 nt) 1e 19 ugs104 MUSMTHYPA L07095 Mus domesticus strain NZB/B1NJ mitochondrion genome, compl (16303 nt) 2.9e-56 ugs178 MUSMTHYPB L07096 Mus domesticus strain MilP mitocondrion genome, complete s (16303 nt) 1.2e-53 RNA Splicing, Binding, RNPs, etc... ug185 MUSCIRPB D78135 Mus musculus mRNA for CIRP, complete cds. 2/98 (1256 nt) 8.2e-16 (CIRP = cold-inducible RNA-binding protein jdr) ug304 HSU97188 U97188 Homo sapiens putative RNA binding protein KOC (koc) mRNA, c (4181 nt) 8.3e-31 *ug485 MUSCIRPB D78135 Mus musculus mRNA for CIRP, complete cds. 2/98 (1256 nt) 7e-09 *ug494cons HUMASF M72709 Human alternative splicing factor mRNA, complete cds. 9/91 (1717 nt) 1.2e-27 ugs060 HSU85510 U85510 Human RNA polymerase II subunit hsRPB4 mRNA, complete cds, (1894 nt) 1.9e-28 ugs102 HSPABII Y08772 H.sapiens PABII pseudogene, poly(A) binding protein. 1/97 (1930 nt) 8.8e-18 ugs106 MMU40654 U40654 Mus musculus U22 snoRNA host gene (UHG) gene, complete sequ (3838 nt) 2.6e-22 (These snoRNAs are co-transcribed with their host pre- mRNAs and released by processing from excised introns. Here we show that, in addition to U22, seven novel fibrillarin-associated snoRNAs, named U25-U31, are encoded within different introns of the unusually compact mammalian U22 host gene (UHG). All seven RNAs exhibit extensive (12-15 nucleotides) complementarity to different segments of the mature rRNAs, followed by a C/AUGA (′U-turn′) sequence. The spliced UHG RNA, although it is associated with polysomes, has little potential for protein coding, is short-lived, and is poorly conserved between human and mouse. Thus, the introns rather than the exons specify the functional products of UHG.) ugs159 HUMU1RNP1 M60779 Human U1 snRNP-specific protein A gene, exon 1.1/95 (495 nt) 2.6e-14 Peptidases, Proteinases, Isomerases, Transferases ug088rcon MM14MMP9 AF022432 Mus musculus matrix metalloproteinase-14 (Mmp14), exons 9 (1242 nt) 2e-38 ug179rcon AF090430 Mus musculus ATP-dependent metalloprotease FtsH1 mRNA, complete c (2654 nt) 9.5e-15 ug426 MAP5PRQMR X62678 M.auratus mRNA for P5 protein. 8/93 (2234 nt) a member of the protein disulphide isomerase/form I phosphoinositide-specific phospholipase C family 3.2e-21 Developmental Unclassified ug109rcon RATDRP L20319 Rattus norvegicus developmentally regulated protein mRNA, com (5395 nt) 3e-60 ug380 MUSMEAA L10401 Mus musculus male-enhanced antigen (Mea) mRNA (human chromo 6p21.1-21.3), complete cds. (841 nt) 1.1e-38 ug423 AF015262 Homo sapiens Down Syndrome critical region, partial sequence. 2/9 (79607 nt) 1.9e-10 ugs008 MMU47024 U47024 Mus musculus maternal-embryonic 3 (Mem3) mRNA, complete cds (3128 nt) 8.8e-12 Protein Turnover ug234 AF071317 Mus musculus COP9 complex subunit 7b (COPS7b) mRNA, complete cds. (1990 nt) 2.2e- 91 (The COP9 complex is conserved between plants and mammals and is related to the 26S proteasome regulatory complexsubunit. 7b is a component of the COP9 complex which contains a total of 8 distinct subunits, similar tothe JAB 1- containing signalosome; the plant COP9 complex functions as a repressor of photomorphogenesis) ug267 MMU96635 U96635 Mus musculus ubiquitin protein ligase Nedd-4 mRNA, complete (5581 nt) 6.7e-50 ug445 AF033353 Mus musculus ubiquitin-homology domain protein (Ubl1) mRNA, compl (1187 nt) 2.1e-87 X Chromosome Associated ug115rcn1o HS23K20 AL022153 Human DNA sequence from clone 23K20 on chromosome Xq25-26. (79472 nt) 4.9e-07 ug196 AC004827 Homo sapiens PAC clone DJ044L15 from Xq23, complete sequence. 10/(79688 nt) 2.5e-25 ug321 MMTSXDNA X99946 M.musculus 94kb genomic sequence encoding Tsx (testis-specific X-chromosome) gene. 11/96 (79555 nt) 2.1e-09 ug328 AB006651 Homo sapiens EXLM1 mRNA, complete cds. 6/98 (7984 nt) 1.1e-116 (Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation) ug385 HSA218J18 AL034370 Human DNA sequence from clone 218J18 on chromosome Xp11. (40465 nt) 1e-19 ug390 HSA218J18 AL034370 Human DNA sequence from clone 218J18 on chromosome Xp11. (40478 nt) 9.5e-19 *ugs194rs AC005859 Homo sapiens Xp22-83 BAC GSHB-324M7 (Genome Systems Human BAC Lib (79502 nt) 3.2e-07 Chromosomal Locus Association ug078rcon AC004132 Homo sapiens chromosome 17, clone hRPC.986_F_12, complete sequence (79432 nt) 2.1e-12 ug089rcon HSAC001228 AC001228 244Kb Contig from Human Chromsome 11p15.5 spanning D11S (79627 nt) 2.4e-21 ug134 HS66H14 Z97989 Human DNA sequence from PAC 66H14 on chromosome 6q21-22. Con (76686 nt) 8.9e-26 ug210c HS82J11 Z83850 Human DNA sequence from PAC 82J11 and cosmid U134E6 on chrom (79596 nt) 3.4e-14 ug286 AC004611 Homo sapiens chromosome 19, cosmid F24200, complete sequence. 4/9 (47055 nt) 2.7e-20 ug323 HS180M12 Z82190 Human DNA sequence from PAC 180M12 on chromosome 22. Contains GSSs (59941 nt) 3e-16 ug346 AC002121 Genomic sequence from Mouse 11, complete sequence. 7/97(79740 nt) 2.2e-11 (poly A jdr) ug350 AC002324 Mus musculus chromosome 11, clone 475_H_14, complete sequence. 5/ (79709 nt) 2.1 e-26 ug364 HUAC002550 AC002550 Human Chromosome 16 BAC clone CIT987SK- A-101F10, comple (79780 nt) 5.9e-15 (poly Ajdr) ug370 HS434P1 Z97056 Human DNA sequence from PAC 434P1 on chromosome 22. Contains (45764 nt) 4.7e-24 ug395 HSL241B9C Z69708 Human DNA sequence from cosmid L241B9, Huntington's Diseas (17243 nt) 1.7e-21 ug402 AP000031 Homo sapiens genomic DNA, chromosome 21q11.1, segment 2/28, compl (79580 nt) 2.6e-10 ug407 AC002116 Human DNA from chromosome 19 cosmid R33743, genomic sequence, com (40491 nt) 4.1e-09 ug450 AC000399 Genomic sequence from Mouse 9, complete sequence. 5/97 (61336 nt) 1.2e-08 ug457 AC003063 Mus musculus Chromosome 16 BAC Clone b40- o20 Syntenic To Homo sap (79720 nt) 2.1 e-11 ug461 AC005807 Mus musculus chromosome 17 BAC clone citb585c7 from MHC region, c (65870 nt) 4.7e-07 ug467 HSU96629 U96629 Human chromosome 8 BAC clone CIT987SK- 2A8 complete sequence (79589 nt) 7.2e-08 ug470 AC003018 Mus musculus Chromosome 4 BAC84c8, complete sequence. 5/98 (57327 nt) 4.8e-10 ug476 AC002324 Mus musculus chromosome 11, clone 475_H_14, complete sequence. 5/ (79604 nt) 1.5e-16 ug477 AC005900 Homo sapiens chromosome 17, clone hRPK.998_F_8, complete sequence (79544 nt) 1.3e-07 ug488 HS459L4 AL031120 Human DNA sequence from clone 459L4 on chromosome 6p22.3-2 (79692 nt) 4.2e-08 ug497 AC002324 Mus musculus chromosome 11, clone 475_H_14 complete sequence. 5/ (79672 nt) 6.8e-11 ug524 MMNHCHMG1 Z11997 M.musculus mRNA for non-histone chromosomal high-mobility (2231 nt) 2.3e-69 ugs017 AC004790 Homo sapiens chromosome 19, cosmid F17987, complete sequence. 6/9 (41613 nt) 4.4e-20 ugs021 HSD13S106 X59131 Homo sapiens D13S106 mRNA for A unique intronless gene or gene seqment on chromosome 13 specifying a highly charged amino acid sequence (3650 nt) 1.5e-12 ugs033 AC005259 Mouse BAC CitbCJ7 219m7, genomic sequence, complete sequence. 7/9 (79776 nt) 5e-09 ugs036 AC005742 Mus musculus chromosome 11, BAC clone 111- 181 (LBNL M01), complet (79780 nt) 7.3e-07 ugs116 AC004500 Homo sapiens chromosome 5, P1 clone 1076B9 (LBNL H14), complete s (77538 nt) 1.3e-26 ugs125 AC004259 Human Chromosome 15q11-q13 PAC clone pDJ14i12 containing Angelman (79744 nt) 2.3e-07 ugs134 AF059580 Murine genomic DNA; partially digested Sau3A fragment, cloned int (36326 nt) 3e-45 ugs139 HS94G16 Z85999 Human DNA sequence from PAC 94G16 on chromosome 6q21. Contai (79812 nt) 5.6e-07 ugs165 AP000021 Homo sapiens genomic DNA, chromosome 21q22.2 (Down Syndrome regio (79776 nt) 3.1e-08 ugs191 AC005070 Homo sapiens BAC clone RG152G17 from 7q22- q31.1, complete sequenc (79788 nt) 4.3e-16 ugs208 AF044773 Homo sapiens breakpoint cluster region protein in uterine leiosarcoma (chromo t12:14) (BCRG1) mRNA, co (772 nt) 2e-12 ugs219 DJ270M14 AF107885 Home sapiens chromosome 14q24.3 clone BAC270M14 transform (79780 nt) 2.3e-11 Heat Shock, Chaperones, Protein Trafficking ug147 HUMCALIEF M94859 Human calnexin mRNA (molecular chaperone), complete cds. 9/94 (3881 nt) 7.8e-22 ug245 RNHSP7O3 X77209 R.norvegicus Hsp70-3 gene. 1/97 (3913 nt) 7.8e-25 ug348 MMSCP2EX6 X91155 M.musculus scp2 gene exon 6. 1/97 (512 nt) 1.2e-21 (the murine sterol carrier protein 2 gene (Scp2)) ug400 MMHSP47 X60676 M.musculus HSP47 mRNA. 6/93 (2273 nt) 4.9e- 104 ug435 AF058718 Homo sapiens putative 13 S Golgi transport complex 90kD subunit brain-specific isoform mRNA, complete cds. (3105 nt) 2.6e-07 ug455 HSU67615 U67615 Human beige protein homolog (chs) mRNA, complete cds. 1/97 (13449 nt) 3.4e-11 (beige gene is involved in protein and lysosomal trafficking) ug507 RATNOP140A M94287 Rattus norvegicus nucleolar phosphoprotein of 140kD, Nopp (3609 nt) 1.7e-08 Molecular chaperone for NTS containing proteins. ugs019 HUMHBP M64098 Human high density lipoprotein binding protein (HBP) mRNA, co (4354 nt) 8.7e-36 Neural Element or Assoc. ug198 AF047384 Rattus norvegicus postsynaptic protein CRIPT mRNA, complete cds. (1435 nt) 2.7e-23 ug261 HSGTHLA1 Y11044 Homo sapiens mRNA for GABA-BR1a (hGB1a) receptor. 10/98 (4220 nt) 3.8e-12 ug333 MUSSPESPEP M55181 Mouse spermatogenic-specific proenkephalin mRNA, complete (1408 nt) 3.2e-57 DNA Repair ug099rcon AF069519 Mus musculus T:G mismatch-specific thymine- DNA glycosylase TDGb i(2859 nt) 1.1e-51 Metabolism (Cytosolic) ug125 RNO010709 AJ010709 Rattus norvegicus gene encoding tyrosine aminotransferase (12460 nt) 5.3e-16 ug266 MUSCATALAA L25069 Mouse catalase mRNA (antioxidant enzyme), complete cds. 5/95 (2423 nt) 1.4e-54 ug366 HSU62961 U62961 Human succinyl CoA: 3-oxoacid CoA transferase precursor (OXC (3337 nt) 3.4e-11 ugs022 MSALEN X52379 Mouse mRNA for alpha-enolase (2-phospho-D- glycerate hydrolase (1720 nt) 2.2e-46 Unknown *ug317 MMU80894 U80894 Mus musculus CAG trinucleotide repeat mRNA, Transcription factor or Cadherin. (543 nt) 6.8e-51 ugs025 AF052130 Homo sapiens clone 23704 mRNA sequence. 8/98 (1810 nt) 1.7e-09 ugs080 MUSHKPRO M74555 Mouse house-keeping protein mRNA, complete cds. 8/91 (2415 nt) 2.7e-54 *ugs506or MMY17106 Y17106 Mus musculus transposon ETn, SELH/L3A strain. 10/98 (5542 nt) 2.5e-73 ugs042 MMY17106 Y17106 Mus musculus transposon ETn, SELH/L3A strain. 10/98 (5542 nt) 5.1e-50 ug160 AB014563 Homo sapiens mRNA for KIAA0663 protein, complete cds. 7/98 (4365 nt) 3.2e-60 ug178rcon AB011125 Homo sapiens mRNA for KIAA0553 protein, partial cds. 4/98 (5574 nt) 2.9e-26 ug209 D86971 Human mRNA for KIAA0217 gene, partial cds. 7/97 (5404nt) 2.4e-42 ug213 D86971 Human mRNA for KIAA0217 gene, partial cds. 7/97 (5404 nt)7.3e-41 ug263 AB014550 Homo sapiens mRNA for KIAA0650 protein, partial cds. 7/98 (5003 nt) 2.4e-79 ug275 HUMORF16 D14812 Human mRNA for KIAA0026 gene, complete cds. 7/97 (1826 nt) 3.2e-45 ug377 HUMORF16 D14812 Human mRNA for KIAA0026 gene, complete cds. 7/97 (1826 nt) 1.5e-66 ug481cp2 AB018325 Homo sapiens mRNA for KIAA0782 protein, partial cds. 11/98 (4130 nt) 5.2e-10 ugs027 AB018272 Homo sapiens mRNA for KIAA0729 protein, partial cds. 11/98 (4143 nt) 4.2e-51 ugs029 AB018306 Homo sapiens mRNA for KIAA0763 protein, complete cds. 11/98 (4148 nt) 1.1e-27 ugs099 AB002293 Human mRNA for K1AA0295 gene, partial cds. 6/97 (7326nt) 6.4e-40 ugs100 D86958 Human mRNA for KIAA0203 gene, complete cds. 7/97 (6614nt) 8.5e-40 ugs211 AB018325 Homo sapiens mRNA for KIAA0782 protein, partial cds. 11/98 (4130 nt) 1.2e-19 ugs235 AB018330 Homo sapiens mRNA for KIAA0787 protein, partial cds. 11/98 (4427 nt) 2.2e-13

[0054] Table 4 presents the results of the library analysis of 787 cDNA UGS-derived ESTs using the GenBank database. TABLE 4 Results of the library analysis of 787 cDNA UGS-derived ESTs using the GenBank database Clone Locus Acc. # Identity GTPases ua1b5 RNARP1 X78603 R.norvegicusSprague DawleyARP1 mRNA faD ARF-related prote (943 nt) 1.3e-19 (ARP is a plasma membrane-associateD Ras-related GTPase with remote similarity to the family oD ADP ribosylation factors.D ua1e1r HSU18420 U18420 Human ras-related small GTP binding protein Rab5rab5mRN (1590 nt) 4.8e-27 ug035con HSPACAP X60435 H.sapiens gene PACAP for pituitary adenylate cyclase activating polypeptide (PACAP) (17041 nt) 9.5e-08 ug182 RATGCA J05677 Rat guanylyl cyclase A/atrial natriuretic peptide receptor G (17517 nt) 9.9e-07 Protein Kinases/Phosphatases ug069rcon HSPTP1CHG X82818 H. sapiens PTP1C/HCP gene.protein tyrosine phosphatase. June 1997 (8545 nt) 2.7e-30 Structural Proteins/ECM ua1e3f MMU49739 U49739 Mus musculus unconventional myosin VIsvmRNAD complete c (4602 nt) 2e-36 ug013rcon RATCRBGLVC L20468 Rattus norvegicus cerebroglycan mRNAD complete cds. January 1994 (2607 nt) 2e-23 ug031con AF078705 Z97056 Mus musculus vascular adhesion protein-1 gene. complete cds. September 1998 (14357 nt) 6.5e-12 ug055con MMLAMBETA2 U43541 Mus musculus laninin beta 2 gene, exon 17-33, (5350 nt) 2.3e-95 ug059 AB009808 AF085906 Homo sapiens gene for osteonidogen, intron 9. March 1998 (9085 at) 2.2e-08 ug464 MMSYNDE1A Z22532 M. musculus syndecan-1. April 1997 (33934 nt) 6.7e-07 Oncogenes/Tumor Suppressors/Apoptosis ug039rcon MMAF000168 AF000168 Mus musculus 9ORF binding protein 19BP-1 mRNA, Binding of Human Virus Oncoproteins to bD1g/SAP97, a Mammalian Homolog of the Drosophila Discs large Tumor Suppressor protein (2703 nt) 2.1e-155 Growth Factors, Cytokines & Binding Proteins ua1a4f AF063020 Z11584 Homo sapiens Lens epithelium-derived growth factor (LEDGF) mRNA (3377 nt) 1.5e-24 ua1e6r MMU7909 AJ007909 Mus musculus mRNA for erythroid differentiation regulator, A novel protein from WEHI-3 cells inducing hemoglobin synthesis in human K562 and murine erythroleukemia cells (715 nt) 3.8e- 16 ua1f5f AF063020 AJ007909 Homo sapiens lens epithelium-derived growth factor mRNA, A novel protein from WEHI-3 cells inducing hemoglobin synthesis in human K562 and murine erythroleukemia cells complete (715 nt) 5.5e-23 ua1g2f MMU7909 AJ007909 Mus musculus mRNA for erythroid differentiation regulator A novel protein from WEHI-3 cells inducing hemoglobin synthesis in human K562 and murine erythroleukemia cells, (715 nt) 2.4e-26 ua2h6r MUSIGFBP04 L05439 Mouse insulin-like growth factor binding protein 2 (IGFBP-2) (532 nt) 3.4e-73 ug051rcon MMTHYMOA X56135 Mouse mRNA for prothymosin alpha. June 1991 (1191 nt) 8.7e-56 Ribosomal Proteins and rRNA ug037rcon MM45SRRNA X82564 M. musculus 45S pre rRNA gene. April 1996 (22118) nt4.6e-99 Transcription Factors/Co-factors ug011rcon MMCNBPMR X63866 Mus musculus mRNA for cellular nucleic acid binding protein (1492 nt) 1.5e-102 ug033con MMTSC22 X62940 M. musculus TSC-22 mRNA. Isolation of a gene encoding a putative leucine zipper structure that is induced by transforming growth factor beta 1 and other growth factors. December 1993 (1706 nt) 6e-128 ug053rcon D87671 X56135 Rat mRNA for TIP120, TATA-binding protein interacting protein. January 1997 (4383 nt) 7.8e-84 *ug092 GGU68380 U68380 Gallus gallus single-strand DNA-binding protein. csdp SSDP (sequence-specific single-stranded DNA-binding protein), mRNA,(1211 nt) 5.2e-85 ugs045 AF056002 Z22532 Rattus norvegicus Smad4 protein Smad4 mRNA, complete cds. April 1998 (3041 nt) 8.3e-36 Mitochondrial ug292 AA933159 D21852 UI-R-E0-cz-e-07-0-UI.s1 UI-R-E0 Rattus norvegicus cDNA clone UI-R (283 nt) 1.9e-33 (Rat mitochondrial genome fragment encoding cytochrome oxidase subunit I) ugs104 MUSMTHYPB L07096 Mus domesticus strain MilP mitocondrion genome, complete seq (16303 nt) 6.3e-48 RNA Splicing, Binding, RNPs, etc . . . ug034con AF015812 X62940 Homo sapiens RNA helicase p68HUMP68gene, complete cds. November 1997 (7834 nt) 4.5e-111 Peptidases, Proteinases, Isomerases, Transferases ug238 HUMCANPRA J04700 Homo sapiens calcium-dependent protease large subunit CAN (1154 at) 8.4e-07 ug406 RNU05013 U05013 Rattus norvegicus Sprague-Dawley heme oxygenase-2 non-reducing form, genomic clone (14984 nt) 1.5e-08 Developmental Unclassified ug251 AU043179 AU043179 Mouse sixteen-cell-embryo cDNA Mus musculus cDNA clone J (550 nt) 3.8e-75 ug373 AI550071 AU051101 mn04d09.y1 Beddington mouse embryonic region Mus musculus cDNA clone (508 nt) 3.3e-65 ugs060 AI331913 Z22532 fa95b11.y1 zebrafish fin day3 regeneration Danio rena cDNA 5′si (476 nt) 5e-34 Protein Turnover ug066rcon MMUBIQU X51703 Mouse mRNA for ubiquitin. May 1991 (1172 nt) 2.8e- 30 X Chromosome Associated ug158 AF002223 L27758 Human genomic DNA of Xq28 with MTM1 and MTMR1 genes, complete seq (79535 nt) 2.8e-13 (MTM1 gene mutations in 47 unrelated X-linked myotubular myopathy patients.) Chromosomal Locus Associated ua1a6r AC004453 Z11584 Homo sapiens PAC clone DJ0844F09 from 7p12- p13D complete sequence (79660 nt) 1e-10 ug021rcon AC003997 L20468 Mouse BAC mbac20 from 14D1-D2T-Cell Receptor AlphD Locus, (79656 nt) 8.1e-56 ug028rcon HS434P1 Z97056 Human DNA sequence from PAC 434P1 oD chromosome 22. Contains (45869 nt) 3.6e-13 ug036rcon AC004079 X62940 Homo sapiens PAC clone DJ0167F23 from 7p15, completD sequence. (23938) nt 7.1e-63 ug048 HS434P1 Z97056 Human DNA sequence from PAC 434P1 on chromosome 22. Contains (45548 nt) 2.1 e-46 ug050rcon AC005742 Z97056 Mus musculus chromosome 11, BAC clone 111- 181LBND M01, complete (79416 nt) 1.3e-10 ug291ft B49438 D21852 RPCI1 1-6118.TV RPCI11 Homo sapiens genomic clone R-6118, genomic (539 nt) 1.9e-08 ug397 HS73M5 AJ010597 Homo sapiens chromosome 21 PAC RPCIP704M573Q2. March 1999 (79664 nt) 7.8e-08 ug429 AC005743 U05013 Mus musculus PAC clone, complete sequence. December 1998 (185548 nt) 9.3e-13 ug472 AQ194542 Z22532 RPCI11-60K21.TJ RPCI11 Homo sapiens genomic clone R-60K21, genomic clone (388 nt) 9.1e-09 ugs007 AQ111639 Z22532 CIT-HSP-2378O4.TF CIT-HSP Homo sapiens genomic clone 2378O4, geno (634 nt) 5.8e-09 ugs018 AU026520 Z22532 Rattus norvegicus, OTSUKA clone, OT83.06/945f02, microsatellite seq. (298 nt) 3.4e- 08 ugs031 AC005259 Z22532 Mouse BAC CitbCJ7 219m7, genomic sequence, complete sequence. July 1997 (9836 nt) 1.5e-07 ugs032 AQ240341 Z22532 CIT-HSP-2386E2.TF.1 CIT-HSP Homo sapiens genomic clone 2386E2, (576 nt) 8.7e-28 ugs043 AC004406 Z22532 Mus musculus ma40a113, complete sequence. T- cell receptor locus. March 98 (47536 nt) 1.9e-09 ugs065 HUAC004531 AC004531 Home sapiens Chromosome 16 BAC cloneD CIT987SK-A-67A1, clone (79783 nt) 3.5e-10 ugs113 AC006087 L07096 ***SEQUENCING IN PROGRESS***Homo sapiens chromosome 12p13.3 clone (79790 nt) 1.5e-15 ugs127 AC002327 AU051628 Mus musculus chromosome 7, clone 19K5, complete sequence. February 1999 (79756 nt) 1.7e-07 ugs138 MMU58105 U58105 Mus musculus Btk locus, alpha-D-galactosidase AAgs, ribosomal protein (L44L), and Bruton's tyrosine kinase (Btk) genes (79780 nt) 4.8e-07 ugs152 AC007110 U58105 Homo sapiens chromosome 17, clone hRPK.472_J_18, complete sequence (79816) nt 1.1e-07 ugs157 AC002109 U58105 Genomic sequence from Mouse 9, complete sequence. September 97 (79776 nt) 1.1e-07 ugs225 AC003949 L27758 Mus musculus chromosome 19, clone D19-96, B7, complete sequence. (769037 nt) 8.5e-09 ugs228 AP000025 L27758 Homo sapiens genomic DNA, chromosome 21q1 1.1, segment 3/5, complete (31709 nt) 5.8e- 23 Heat Shock, Chaperones, Protein Trafficking ugs203 AF086628 L27758 Homo sapiens Vesicle associated membrane protein (VAMP)-associated protein BVAP-B mRNA, complete cds (2195 nt)1.7e-15 Testis/Sperm or Male enhanced ua2h7r D78270 X60435 Mouse mRNA for male-enhanced antigen-2, complete cds. April 1997 (4621 nt) 1.4e-07 ug058rcon MMTSXDNA X99946 M. musculus 94kb genomic sequence encoding TsD gene. November 1996 (79563 nt) 2.7e-12 (a new testis specific gene TsxD) ug197 AQ212110 J05677 HS_3241_B1_E05_MR CIT Approved Human Genomic Sperm Library Homo sapiens (395 nt) 6.9e-09 ugs078 AQ303203 AC004531 HS_3235_B2_H09_T7 CIT Approved Human Genomic SpermD Library Homo (504 nt) 5.1 e-37 ugs195 AQ270425 AL034550 HS_2052_B1_H06_T7 CIT Approved Human Genomic Sperm Library D Homo (380 nt) 2.6e-23 Metabolism (Cytosolic) ugs080 MUSHKPRO M74555 Mouse house-keeping protein mRNA, complete cds. August 1991 (2415 nt) 3.7e-57 Normalized Library ESTs (Non-Human) ug073rcon HUMYQ60A05 AF085906 Homo sapiens full length insert cDNA clone YQ6OA05. August 1998 (497 nt) 5.3e-15 ug478 AI384054 Z22532 te36a06.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:2088754 (488 nt) 2.4e-40 ug483 AI269337 Z22532 qj69d02.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1864707 3′(416 nt) 3.3e-23 ugs145 AA724439 U58105 ah91h04.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE: 1326 (428 nt) 4.4e- 20 ugs181 HS1184F4 AL034550 Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone (79790 nt) 9.5e-24 ugs183 AI521602 AL034550 to65e01.x1 NCI_CGAP_Gas4 Homo sapiens cDNA clone IMAGE:2183160 3′ (434 nt) 9.5e-09 ugs200 AI525836 C83432 PT1.3_06_D04.r tumor1 Homo sapiens cDNA 5′, mRNA sequence. March 1999 (913 nt) 6.8e-16 ugs236 AI346524 L27758 qp51d11.x1 NCI_CGAP_Co8 Homo sapiens cDNA clone IMAGE: 1926549 3′ (702 nt) 1.4e-29 Normalized Library ESTs (Non-Human) ua1f3fr AI407830 X60435 EST236120 Normalized rat ovary, Bento Soares Rattus sp cDNA clone (553 nt) 6.2e-21 ua1a4r AI510687 X60435 vx91h09.y1 Soares 2NbMT Mus musculus cDNA clone IMAGE:1282625 5′, (446 nt) 1e-22 ug004rcon AI103952 X60435 EST213241 Normalized rat heart, Bento Soares Rattus sp. cDNA clone (522 nt) 6.5e-41 ug0l0rcon AI071801 X60435 UI-R-C2-nj-h-11-O-UI.s1 UI-R-C2 Rattus norvegicus cDNA clone UI-R from 8-day embryo normalized library (218 nt) 3.2e-22 ug017rcon AI177121 X60435 EST220728 Normalized rat ovary, Bento Soares Rattus sp. cDNA clone (362 nt) 6.7e-36 ug022rcon AI415663 X60435 mc65f04.x1 Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA clone (456 nt) 2.7e-54 ugo24rcon AI337885 X60435 qt34d08.x1 Soares_pregnant_uterus_NbHPU Homo sapien cDNA clone I(516 nt) 7e-80 ugo43rcon AI465001 AF085906 vw55b07.y1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone (470 nt) 3.6e-3 1 ug091rcon AI406675 X60435 EST234962 Normalized rat ovary, Bento Soares Rattus sp. cDNA clone (489 nt) 2.4e-15 *ug096ors AI556610 U68380 UI-R-C2p-ri-d-03-0-UI.s1 UI-R-C2p Rattus norvegicus cDNA clone (431 nt) 3e-25 ug144 AI463953 L27758 vw67d10.y1 Stratagene mouse heart #937316 Mus musculus cDNA clone (448 nt) 5.3e-59 ug161 AI179287 L27758 EST222980 Normalized rat spleen, Bento Soares Rattus sp. cDNA clone (559 at) 2.1e-37 ug162 AI454888 L27758 UI-R-C2p-q1-e-06-0-UI.s1 UI-R-C2p Rattus norvegicus cDNA clone (375 nt) 1.5e-45 ug163rcon AI462455 L27758 ub73a04.x1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone (459 nt) 9.6e-92 ug169rcon AI555802 L27758 UI-R-C2p-qz-e-01-0-UI.s1 UI-R-C2p Rattus norvegicus cDNA clone (515 nt) 5e-100 ug183rcon AI466568 J05677 vx79f07.y1 Soares 2NbMT Mus musculus cDNA clone IMAGE:1281445 5′, (180 nt) 4.2e-41 ug212 AI179029 J05677 EST222711 Normalized rat spleen, Bento Soares Rattus sp. cDNA clone (464 nt) 3.9e-16 ug221 AI462455 J05677 ub73a04.x1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone (459 nt) 2.8e-83 ug226 AI386220 J05677 mq66d04.y1 Soares 2NbMT Mus musculus cDNA clone IMAGE: 583687 5′s (608 nt) 6.2e- 91 ug237 AI467092 J05677 vd64a07.x1 Knowles Solter mouse blastocyst B1 Mus musculus cDNA clone (358 nt) 2.4e-74 ug255 AA963280 AU043179 UI-R-E1-gh-h-04-0-UI.s1 UI-R-E1 Rattus norvegicus cDNA clone UI-R (409 nt) 1.2e-43 ug280 AI551859 937311 vo93h08.x1 Soares mouse mammary gland NbMMG Mus musculus cDNA clone (348 nt) 1.4e-48 ug301 AI414548 D21852 ma46c09.x1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE: (3396 nt) 2.2e-67 ug318 AU051101 AU051101 Sugano mouse brain mncb Mus musculus cDNA clone MNCb-152918 nt 1.Se-31 ug342 AA782146 AU051101 ai48f10.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone (421 nt) 2.5e-32 ug345 AI059369 AU051101 UI-R-C1-1d-e-09-0-UI.s1 UI-R-C1 Rattus norvegicus cDNA clone UI-R (379 nt) 2.4e-24 ug352 AI536347 AU051101 ma93g05.y1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE: (3638 nt) 2.7e- 12 *ug357 AI428736 AU051101 vv49a04.y1 Soares 2NbMT Mus musculus cDNA clone IMAGE:1225710 5′(463 nt) 1.8e-90 ug358 AI071119 AU051101 UI-R-C2-mt-a-08-0-UI.s1 UI-R-C2 Rattus norvegicus cDNA clone UI-R (369 nt) 7.9e-36 ug359 AA998224 AU051101 UI-R-C0-ib-d-09-0-UI.s1 UI-R-C0 Rattus norvegicus cDNA clone UI-R (328 nt) 5.6e-36 ug383 AI227819 AU051101 EST224514 Normalized rat brain, Bento Soares Rattus sp. cDNA clone (395 nt) 3.4e-49 ug384 AA963776 AU051101 UI-R-E1-gk-f-01-0-UI.s1 UI-R-E1 Rattus norvegicus cDNA clone UI-R (443 nt) 1.4e-24 ug388 AI011736 AU051101 EST206187 Normalized rat ovary, Bento Soares Rattus sp. cDNA clone (638 nt) 2.6e-43 ug393 AI547803 AU051101 UI-R-C3-sk-a-09-0-UI.s1 UI-R-C3 Rattus norvegicus cDNA clone UI-R (513 nt) 1.2e-29 ug396 AI347278 AU051101 tc05d06.x1 NCI_CGAP_Co16 Homo sapiens cDNA clone IMAGE: 3′, mRNA (465 nt) 1.3e-24 ug399 AI315959 AJ010597 uj28b10.y1 Sugano mouse kidney mkia Mus musculus cDNAD clone IMAGE (642 nt) 5e-46 ug404 AI314760 AJ010597 uj28d10.x1 Sugano mouse kidney mkia Mus musculus cDNAD clone IMAGE (686 nt) 1.2e-35 ug421 AI102962 U05013 EST212251 Normalized rat embryo, Bento Soares Rattus sp. cDNA clone (509 nt) 1.6e-67 ug434 AI323275 U05013 mp96d04.y1 Soares 2NbMT Mus musculus cDNA clone IMAGE:577063 5′ (487 nt) 3e-09 ug436 AI503731 U05013 vk81e11.x1 Knowles Solter mouse 2 cell Mus musculus cDNA clone IMAGE (496 nt) 1.4e-17 *ug440rs AI465094 U05013 vw65h07.y1 Stratagene mouse heart#937316Mus musculus cDNA clone (425 nt) 1.4e-47 ug456 AI451948 U05013 mp75e08.x1 Soares 2NbMT Mus musculus cDNA clone IMAGE:575078 3′,374 nt 5.7e-72 ug471 AI071177 Z22532 UI-R-C2-my-d-06-0-UI.s1 UI-R-C2 Rattus norvegicus cDNA clone UI-R (472 nt) 2.2e-51 *ug482 AI230701 Z22532 EST227396 Normalized rat embryo, Bento Soares Rattus sp. cDNA clone (420 nt) 2.3e-23 ug496 AI172198 Z22532 EST218195 Normalized rat muscle, Bento Soares Rattus sp. cDNA clone (642 nt) 1.4e-20 ug504 AI503116 Z22532 vm94a04.x1 Knowles Solter mouse blastocyst B1 Mus musculus cDNA clone (385 ut) 6e-69 *ug505ors AI314057 Z22532 uj25a12.x1 Sugano mouse kidney mkia Mus AA673668 musculus cDNA clone IMAGE (635 nt) 3.5e-45 or 10667012 vo57h10.r1 Soares mouse mammary gland NbMMG Mus musculus cDNA clo 547 at 6.6e-24 ug519 AI463740 Z22532 va16g07.y1 Soares mouse lymph node NbMLN Mus musculus cDNA clone (526 at) 1.5e-13 ugs006 AI045419 Z22532 UI-R-C1-kg-f-08-0-UI.s1 UI-R-C1 Rattus norvegicus cDNA clone UI-R (319 nt) 1.1e-07 ugs039 AI236146 Z22532 EST232708 Normalized rat ovary, Bento Soares Rattus sp. cDNA clone (612 nt) 1.6e-44 ugs046 AI235046 Z22532 EST231608 Normalized rat ovary, Bento Soares Rattus sp. cDNA clone (484 at) 3.5e-30 ugs048 AI412981 Z22532 EST241281 Normalized rat brain, Bento Soares Rattus sp. cDNA clone (510 nt) 5.1e-23 ugs077 AA924175 AC004531 UI-R-E0-bp-c-02-0-UI.s3 UI-R-E0 Rattus norvegicus cDNA clone UI-R (463 at) 2e-12 ugs085 AI551460 M74555 mp87c07.y1 Soares 2NbMT Mus musculus cDNA clone IMAGE:576204 5′, (406 at) 3.4e-33 ugs092 AI467480 M74555 vd57h06.x1 Knowles Solter mouse blastocyst B1 Mus musculus cDNA clone (398 nt) 2e-12 ugs093 AI482527 M74555 vg49d04.x1 Soares mouse mammary gland NbMMG Mus musculus cDNA clone (420 nt) 7.2e-52 ugs103 AI391039 M74555 mc10h07.y1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE: (3553 at) 1.2e-12 ugs120 AA933159 L07096 UI-R-E0-cz-e-07-0-UI.s1 UI-R-E0 Rattus norvegicusD cDNA clone UI-R283 nt 5.5e-30 ugs122 AU051628 AU051628 Sugano mouse brain mncb Mus musculus cDNA clone MNCb-2261 (781 at) 5.2e-12 ugs129 AI555441 AU051628 UI-R-C2p-qp-h-10-0-UI.s1 UI-R-C2p Rattus norvegicus cDNA clone UI (377 at) 7.5e-22 ugs136 AI227835 AU051628 EST224530 Normalized rat brain, Bento Soares Rattus sp. cDNA clone (552 at) 2.8e-21 ugs140 AI466881 U58105 mz55d06.y1 Barstead mouse pooled organs MPLRB4 Mus musculus cDNA (484 at) 1.6e-14 ugs143 AI481908 U58105 vh18g12.x1 Soares mouse mammary gland NbMMG Mus musculus cDNA clone (267 nt) 2.2e-25 *ugs148 AI415455 U58105 mc57e02.x1 Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA clone (424) nt 9.5 e-45 ugs175 AI510452 U58105 mp96g07.y1 Soares 2NbMT Mus musculus cDNA clone IMAGE:577116 5′(435 nt) 7.4e-46 ugs199 AA818528 C83432 UI-R-A0-au-d-06-0-UI.s1 UI-R-A0 Rattus norvegicus cDNA clone UI-R (611 nt) 2.7e-30 ugs202 AI316156 L27758 uj25fD8.y1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE (663 nt) 9.1e-49 ugs214 AI505916 L27758 vk69c10.x1 Knowles Salter mouse 2 cell Mus musculus cDNA clone IMAGE (587 nt) 3.7e-12 ugs221 AI463931 L27758 vw70b08.y1 Stratagene mouse heart#937316Mus musculus cDNA do (402 nt) 3.3e-18 Unknown *ug120 AC005835 U68380 Mus musculus clone UWGC:mbac82 from 14D1- D2, T-Cell Receptor Alpha (79548 nt) 3.2e-20 OR WORKING DRAFT SEQUENCE, 17 unordered pieces. March 1999 (79545 nt) 9e-18 ug276 AI504762 937311 v117h02.x1 Stratagene mouse T cell Mus musculus cDNA clone (504 nt) 8e-111 ug298 AC005992 D21852 *** SEQUENCING IN PROGRESS *** HTGS phase 1, 13 unordered pieces.(79759 nt) 2.8e-10 ug428 AC004821 U05013 *** SEQUENCING IN PROGRESS *** Homo sapiens clone DJ0098O22; HTGS (79478 nt) 2.7e-22 ugs198 C83432 C83432 rabbit corneal endothelial cell Oryctolagus cuniculus cDNA clone (361 nt) 1.3e-39 ug138 BIACOMGEN L27758 Birmingham IncP-alpha plasmidR18, R68, RK2, D RP1, RP4co (60099 nt)2.2e-10 ug239 BIACOMGEN L27758 Birmingham IncP-alpha plasmidR18, R68, RK2, D RP1, RP4co (60099 nt) 4.9e-32 ug315 BIACOMGEN L27758 Birmingham IncP-alpha plasmidR18, R68, RK2, RP1, RP4co (60099 nt)1.4e-18 ugs201 BIACOMGEN L27758 Birmingham IncP-alpha plasmidR18, R68, RK2, RP1, RP4co (60099 nt)4e-29 ua1e3r AB002387 U49739 Human mRNA for KIAA0389 gene, complete cds. June 1997 (5212 nt) 6.1e-29 ua1g4r HUMORF05 D14661 Human mRNA for KIAA0105 gene, complete cds. July 1997 (1622 nt) 6.4e-49 ug282 HUMORFIA D21852 Human mRNA for KIAA0029 gene, partial cds. July 1997 (4272 nt) 3.4e-08

[0055] Table 5 presents the results of the library analysis of 787 cDNA UGS-derived ESTs using the GenBank expressed sequence tag database. TABLE 5 Results of the library analysis of 787 cDNA UGS-derived ESTs using the GenBank expressed sequence tag database Clone Locus Acc. # Identity (Source Tissue or Near Match) Protein Kinases/Phosphatases ug003 AA552488 nk12e05.s1 NCI_CGAP_Co2 Homo sapiens cDNA clone IMAGE:1013312 3′similar to gb:L07395 Protein Phosphatase PP1-Gamma Catalytic Subunit (HUMAN);contains element MER35 repetitive element mRNA sequence (600 nt) 7.5e-07 Growth Factors Induced ugs068 H33545 10667012 EST109665 Rat PC-12 cells, NGF-treated 9 days Rattus sp. cDNA clone (291 nt)3.1e-33 Transcription Factors/Co-factors ug171rcon AI060775 d08016 ub43e04.r1 Soares 2NbMT Mus musculus cDNA clone IMAGE:1380510 5′end similar to WP:F13H6.1 CE09373 Zinc-Finger Protein; (388 nt) 8.1e-19 Egg (Fertilized) ug186rcon C88119 Mouse fertilized one-cell-embryo cDNA Mus musculus cDNA clan 567 nt 2.4e-46 Egg (Unferrtilized) ug185rcon AU023398 AU023398 Mouse unfertilized egg cDNA Mus musculus cDNA clone J043 585 nt 9.5e-15 Two-Cell Embryo ua1c1 AA536741 vj88c04.r1 Knowles Solter mouse 2 cell Mus musculus cDNA clone IM 603 nt 1.2e-38 ug110rcon AU016170 Mouse two-cell stage embryo cDNA Mus musculus cDNA clone 596 nt 1.6e-07 Blastocyst ugs233 AA590398 937311 vm16b09.r1 Knowles Solter mouse blastocyst B1 Mus musculus cDNA c 521 at 2.1e-29 Fetus (7.5d pc) ug005rcon AA120195 mn34h12.r1 Beddington mouse embryonic region Mus musculus cDNA clone 7.5d pc (485 nt) 2.9e-42 ug216 AA409017 10667012 EST03497 Mouse 7.5 dpc embryo ectoplacental cone cDNA library Mus 475 nt 3.2e-26 Fetus (13.5-14.5d pc) ua1f4f AA051759 mj54d09.r1 Soares mouse embryo NbME 13.5 14.5 Mus musculus cDNA c1 455 nt 9.5e-27 Fetus (15.5d pc) ug210 AA288823 10667012 mr51a03.r1 Life Tech mouse embryo 15 5dpc Mus musculus c 212 nt4.8e-19 Fetus-Human (9 wk) ug398 AA451660 10667012 zx43f06.r1 Soares_total_fetus_Nb2HF8_9wk Homo sapiens cDNA clone I (471 nt) 2.3e-54 Brain ug223 AA982689 10667012 uh12b11.r1 Soares mouse hypothalamus NMHy Mus musculus cDNA clone 243 nt 1.4e-58 ug233 Z46187 10667012 HSC26A061 normalized infant brain cDNA Homo sapiens cDNA clone c-26 294 nt 6.4e-12 ugs196 R12838 937311 yf57g11.r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE:2 410 nt 9.6e-34 Breast ug132 R75784 AU016170 y121d10.r1 Soares breast 2NbHBst Homo sapiens cDNA clone IMAGE:1588 616 nt 3.9e-10 ug443 AA463093 10667012 vf92h09.r1 Soares mouse mammary gland NbMMG Mus musculus cDNA clo 445 nt 1.5e-15 Heart ug009rcon AA512195 vj21d02.r1 Soares mouse NbMH Mus musculus cDNA clone from heart IMAGE:922371 (434 nt) 1.1e-31 Liver/Spleen ug143 R94675 d08016 yq42g09.r1 Soares fetal liver/spleen 1NFLS Homo sapiens cDNA clone (396 nt) 1.4e-14 ug191rcon AA177621 C88119 mt32e09.r1 Soares mouse 3NbMS Mus musculus cDNA clone from 4wk spleen IMAGE:62279 (517 nt) 2.2e-66 Macrophage/T cells ug025rcon AA896565 vx63h11.r1 Stratagene mouse macrophage #937306 Mus musculus cDNA (542 nt) 2.4e-42 ug431 AA896311 10667012 vy13b07.r1 Stratagene mouse macrophage #937306 Mus musculus cDN (371 nt)9.5e-41 ug463 AA981302 10667012 vx60a04.r1 Stratagene mouse macrophage #937306 Mus musculus cDN (423 nt)2.6e-61 ugs108 AA655870 937311 vs41h04.r1 Stratagene mouse T cell Mus musculus cDNA clone (403 nt) 1.3e-55 Myotubes ug314 AA815998 10667012 vr14b11.r1 Barstead mouse myotubes MPLRBS Mus musculus cDNA clone (603 nt) 1.8e-36 ug343 AA754682 10667012 vu20e09.r1 Barstead mouse myotubes MPLRB5 Mus musculus cDNA clone (472 nt) 3 .9e-59 ugs161 AA521515 937311 vi07b05.r1 Barstead mouse myotubes MPLRB5 Mus musculus cDNA clone (602 nt) 7.7e-59 Ovary/Female Reproductive ug516 AA429173 10667012 zv49e04.r1 Soares ovary tumor NbHOT Homo sapiens cDNA clone IMAGE (492 bt) 2e-14 ugs070 AA338077 10667012 EST42893 Endometrial tumor Homo sapiens cDNA 5′end similar to small nuclear ribonucleoprotein, polypeptide C, mRNA sequence 293 nt 4.9e-29 Retina *ug491ft W26371 10667012 26f7 Human retina cDNA randomly primed sublibrary Homo sapiens cDNA (621 nt) 8.5e-29 Testis/Male Reproductive ug064rcon AA139248 mr69b12.r1 Stratagene mouse testis #937308 Mus musculus cDNA c1 (347 nt)1e-10 ug137rcon T19209 d08016 d08016t Testis 1 Homo sapiens cDNA clone 5′ end, mRNA sequence (397 nt) 2.9e-12 ug184rcon AI115344 d08016 uh84a11.r1 Soares mouse urogenital ridge NMUR Mus musculus cDNA c (417 nt) 7.7e-69 Thymus ugo30rcon AA120338 mp89a02.r1 Soares 2NbMT Mus musculus cDNA clone from 4 wk thymus IMAGE:576362 5′, (334 nt) 2.5e-39 ug295 AA209880 10667012 mu40c07.r1 Soares 2NbMT Mus musculus cDNA clone 4wk thymus IMAGE:641868 5′, (501 nt) 3.8e-106 ug418 AI049035 10667012 ub39b04.r1 Soares 2NbMT 4 wks Mus musculus cDNA clone IMAGE: 1380079 5′cDNA from Thymus, (530 nt) 2.1e-71 ugs011 AI060722 10667012 ub42h03.r1 Soares 2NbMT Mus musculus cDNA clone from 4wk fetal thymus IMAGE:1380437 5′end, (457 nt) 2.9e-38

[0056] Table 6 presents a summary of the urogenital sinus clone unknowns. TABLE 6 List of Urogenital Sinus clone unknowns UGS Clone Unknowns N = 157 ua1a6f ua1b4 ua1d2 ua1e5r ua1f1r ua1f6 ua1g5f ua1h4 ug003meld ug006rcon ug008rcon ug012rcon ug015rcon ug018rcon ug020r2 ug026rcon ug032rcon ug041rcon ug044rcon ug046 ug047rcon ug054 ug071rcon ug074rcon ug075rcon ug079rcon ug085rcon ug090rcon ug096rcon ug097rcon ug098rcon *ug106rcon ug107rcon ug112 ug113rcon ug115rcon ug117 ug118 ug121 ug123 ug128 ug130r2 ug146 ug148 ug151rcon ug152rcon ug154rcon ug173rcon ug175rcon ug177rcon ug189rcon ug190rcon ug203 ug208 ug217 ug227 ug229 ug230 ug241 ug242 ug247 ug250 ug253 ug254f ug259 ug262 ug269 ug272 ug273 ug274 ug277f ug285 ug288 ug294 ug299 ug305 *ug320 ug322 ug330 ug331 ug332 ug338 ug339 ug341 ug344 ug351 *ug353 ug360 ug368 ug372 ug387 ug403 ug417 ug424 ug430 ug432 ug433 ug437 ug439 ug446 ug452 ug466 ug473 *ug484 ug492 ug495 ug500 ug501 ug508 ug511 ug514 ug520 ugs001 ugs003 ugs009 ugs012 ugs013 ugs014 ugs028 ugs034 ugs035 ugs040 ugs041 ugs047 ugs050 ugs051 ugs052 ugs054 ugs066 ugs067 ugs071 ugs074 ugs084 ugs086 ugs087 ugs088 ugs111 ugs118 ugs121 ugs131 ugs144 ugs150 ugs151 ugs156 ugs163 ugs168 ugs172 ugs173 ugs179 ugs182 ugs184 ugs187 ugs193 ugs204 ugs212 ugs229 ugs231

[0057] Table 7 presents the summary of the 33 clones obtained from the library contig subtraction analysis of all 787 cDNA UGS-derived ESTs cDNA clones. TABLE 7 List of Potential Differentially Expressed UGS Clones by Database SwissProt Match GTPases *ug307cons GBLP_HUMAN P25388 homo sapiens (human), mus musculus (mouse) guanine nucleotide binding protein (317 aa) 3.2e-57 *ug308t GBLP_HUMAN P25388 homo sapiens (human), mus musculus (mouse) guanine nucleotide binding protein (317 aa) 8.8e-55 Protein Kinases *ugs186oft CLK1_MOUSE P22518 mus musculus (mouse). protein kinase clk (ec 2.7.1.-) ( (483 aa) 5.7e-08 (see also clk-4 AF033566 (1549 nt) 9.7e-39) Ribosomal Proteins and Translation *ug334 SR14_MOUSE P16254 mus musculus (mouse). signal recognition particle 14kd (110 aa) 6.9e-42 *ug354cons RLA2_HUMAN P05387 homo sapiens (human). 60s acidic ribosomal protein p2. (115 aa) 1.2e-29 Transcription Factors *ug277t HXAD_AMBME P50210 ambystoma mexicanum (axolotl). homeotic protein hox-a13 (107 aa) 1.2e-34 (other locus 1399859 Acc #U59322) RNA Splicing, Binding, RNPs, etc . . . *ug311cons PSF_HUMAN P23246 homo sapiens (human). ptb-associated splicing factor (ps (707 an) 1.7e-25 *ug4850rs RNPL_HUMAN P98179 homo sapiens (human). putative rna-binding protein rnpl (157 aa) 3.1e-12 Peptidases, Proteinases, Isomerases, Transferases *ug101rcon DPP4_MOUSE P28843 mus musculus (mouse). dipeptidyl peptidase iv (ec 3.4.1) (760 aa) 5.7e-07 *ug335 NEP_RAT P07861 rattus norvegicus (rat). neprilysin (ec 3.4.24.11) (neutral endopeptidase) (749 aa) 5e-20 Hypothetical *ug093rcon YO11_MOUSE P11260 mus musculus (mouse). hypothetical protein orf-1137. (L1Md domain protein, repetitive element retroposon-like) 7/(379 aa) 4.4e-46 GenPent Matches Unknown *ug371f 1480863 U63332 super cysteine rich protein; SCRP Homo sapiens (46 aa) 1.9e-08 (expression appears ubiquitois) GenBank Primate/Rodent Protein Kinases *ug441ors AF027504 Mus musculus putative membrane-associated guanylate kinase 1 (Mag(919 nt) 1.8e-2 Structural Proteins/ECM *ug102cons RATCTTG M80829 Rat troponin T cardiac isoform gene, complete cds. September 1996 (19185 nt) 2.3e-12 (Highly repetitive jdr) Oncogenes/Tumor Suppressors/Apoptosis *ug503s AF017989 Mus musculus secreted apoptosis related protein 1(Sarp1) mRNA, c (2031 nt) 8.3e-57 Membrane Proteins/Receptors *ug254 MUSBA D82019 Mouse gene for basigin, complete cds (exon 1- 7). February 1997 (11763 nt) (Basigin, a new, broadly distributed member of the immunoglobulin superfamily, has strong homology with both the immunoglobulin V domain and the beta-chain of major histocompatibility complex class II antigen.) January 1998 (1302 nt) 5.9e-95 *ug493ors MMEZR X60671 M. musculus mRNA for ezrin. August 1996 (2701 nt) (A gene family consisting of ezrin, radixin and macsin. Its specific localization at actin filament/plasma membrane association sites.)2.9e-57 RNA Splicing, Binding, RNPs, etc . . . *ug485 MUSCIRPB D78135 Mus musculus mRNA for CIRP, complete cds. February 1998 (1256 nt) 7e-09 *ug494cons HUMASF M72709 Human alternative splicing factor mRNA, complete cds. September 1991 (1717 nt) 1.2e-27 X Chromosome Associated *ugs194rs AC005859 Homo sapiens Xp22-83 BAC GSHB-324M7 (Genome Systems Human BAC Lib (79502 nt) 3.2e-07 Unknown *ug317 MMU80894 U80894 Mus musculus CAG trinucleotide repeat mRNA, Transcription factor or Cadherin. (543 nt) 6.Se-51 *ug506or MMY17106 Y17106 Mus musculus transposon ETn, SELH/L3A strain. October 1998 (5542 nt)2.5e-73 GenBank All other database except ESTs Transcription Factors/Co-factors *ug092 GGU68380 U68380 Gallus gallus single-strand DNA-binding protein. csdp SSDP (sequence-specific single-stranded DNA- binding protein), mRNA,(1211 nt) 5.2e-85 Normalized Library ESTs (Non-Human) *ug096ors AI556610 U68380 UI-R-C2p-ri-d-03-0-UI.S1 UI-R-C2p Rattus norvegicus cDNA clone (431 nt) 3e-25 *ug357 AI428736 AU051101 vv49a04.y1 Soares 2NbMT Mus musculus cDNA clone IMAGE:1225710 5′(463 nt) 1.8e- 90 *ug440rs AI465094 U05013 vw65h07.y1 Stratagene mouse heart#9373l6Mus musculus cDNA clone (425 nt) 1.4e-47 *ug505ors AI314057 Z22532 uj25a12.x1 Sugano mouse kidney mkia Mus AA673668 musculus cDNA clone IMAGE (635 nt) 3.5e-45 or 10667012 vo57h10.r1 Soares mouse mammary gland NbMMG Mus musculus cDNA clo 547 nt 6.6e-24 *ugs148 AI415455 U58105 mc57e02.x1 Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA clone (424) nt 9.5e- 45 Unknown *ug120 AC005835 U68380 Mus musculus clone UWGC:mbac82 from 14D1-D2, T-Cell Receptor Alpha (79548 nt) 3.2e-20 OR WORKING DRAFT SEQUENCE, 17 unordered pieces. March 1999 (79545 nt) 9e-18 GenBank ESTs May 3, 1999 Retina *ug491ft W26371 10667012 26f7 Human retina cDNA randomly primed sublibrary Homo sapiens cDNA (621 nt) 8.5e-29 Did Not Match Anything-Truly Unidentified *ug106rcon *ug320 *ug353 *ug484

[0058] Table 8 presents a summary of the library contig subtraction analysis for the 728 cDNA UGS-derived ESTs which reveals 33 differentially expressed UGS-derived EST-containing fetal prostate genes as well two potential homeobox proteins. TABLE 8 Potentially Differentially Expressed Clones Conti Sum Contig g sz Forw or fragme Rev or fragme (F + R) Clone (Y/N) (bp) T7 (bp) nts Sp6 (bp) nts (bp) Comments ug092 N 499 FT 594 ORS 1093 GC rich on rev end ug093 N 165 FT 349 ORS 514 ug096 N 96 F 533 RS 629 GC rich on rev end ug101 Y 503 ORSFT 503 130 bp overlap/120 bp one run ug102 Y 1133 ORSFT 1133 63 bp overlap ug106 N 312 FT 294 ORS 606 ug120 N 87 FFT 444 OS 531 cdx-1? or T cell receptor 168 R R and OS don't align ug254 N? 29 F 461 ORS 490 29 has pT and 461 has pA ug277 N 514 T 409 ORS 923 hoxal3 104 F T7 and F do not align ug291 N 408 FFFT 409 ORS 817 ug307 Y 524 ORSFT 524 160 bp Overlap/ pT on ORS ug308 N 440 T 403 O 843 77 F pT no overlap with T7 ORSSP ug311 Y 704 T 704 110 bp overlap ug317 Y 645 ORSTT 645 250 bp overlap ug320 N 279 FT 560 ORS 839 ug334 N 100 FT 572 ORS 672 pT on FT ug335 N 248 T 156 ORS 404 pT on OR ug353 N 92 FT 633 ORS 725 pT on ft ug354 Y 445 ORSPT 445 pT on ORS ug357 N 225 FT 428 ORS 653 72 bp FF(TRI) overlap/FF(TRI) pb ug371 Y 612 140 ORSF 752 alignment hoxd11 ug440 N 41 F 217 RS 258 pT on F no orig data ug441 N 290 FT 338 ORS 628 no orig data ug482 N 213 FT 330 ORS 543 ug484 N 234 FT 419 ORS 653 ug485 N 153 T 622 ORS 775 ug491 N 603 FT 394 ORS 997 ug493 N 205 FT 263 ORS 468 pT on FT ug503 N 159 FFTT 310 R 469 pT on FT/ 276 S RS do not align ug505 N 180 FFTT 260 ORSS 440 FFF(tri) ug506 N 204 TT 307 OR 511 pT on F 0 ugs148 N 279 OFT 292 RS 571 ugs186 N 410 OFT 238 S 648 ugs194 N 391 OFT 490 RS 881

[0059] These aforementioned 33 cDNA clones can be found in the accompanying tables and figures and are represented herein by the following designations: ug092, ug093, ug096, ug101, ug102, ug106,ug120, ug254, ug291,ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.

[0060] These aforementioned 33 clones have been used herein to identify human paralogs for prostate cancer progression using the LNCaP (androgen dependent, non-tumorigenic) and lineage derived C4-2 (androgen-independent, tumorigenic metastatic to bone) cell line model. Similarly, these 728 fetal UGS-derived cDNA clones could be used to identify other human paralogs involved in the development of prostate diseases including, without limitation, prostatitis, and benign and malignant growth of the prostate gland. “Human paralogs”, as used herein, is intended to mean the human equivalent or homologous sequence.

[0061] These aforementioned 33 clones may be used to identify the aggressiveness of prostate cancer by nucleic acid hybridization techniques or via immunological detection by antisera specific to the gene product. The 33 clones may also be used to develop therapeutic modalities including: tissue- or cancer-specific gene promoters for use in gene therapy by naked DNA delivery; viral toxic gene therapy growth suppression of prostate cancer by replacement gene therapy; tissue specific gene products may also be used to develop immunotherapeutic agents using peptide specific anti-prostate cancer vaccines or adoptive immunotherapies using peptide/protein specific cytotoxic T-cells. Additional cDNA clones may be identified from the 787 UGS-derived ESTs with comparable utility.

[0062]FIG. 8 represents the urogenital sinus fetal prostate cDNA clone summary obtained from GelView Contig run: A determination of the range of independent sequences. 787 cDNA clones were examined which generated 728 usable sequences as acquired. The redundancy was in the range of 2-27 times whereas the average redundancy was 2.84 times. In summary, 66 sequences. max. −44 min. sequences were represented in a contig. of 2 sequences: 33 times (11 contigs. questionable). 24 sequences max. −17 min. sequences were represented in a contig of 3 sequences: 8 times (7 seq. questionable). 5 sequences were represented in a contig. of 5 sequences: 1 time (none questionable). 27 seq. were represented in a contig. of 27 sequences: 1 time (none questionable). Therefore, this result represents 43 generated contig events representing 122 sequences max. and 93 sequences min. in overlapping contigs. Thus, the max. number of single representation is: 728−93=635 single clones+43 seq. contigs.=678 individual sequences. Thus, the min. number of single representation is: 728−122=606 single clones+43 sequences contigs.=649 individual sequences.

[0063]FIG. 9 depicts the additional consensus sequence of differentially expressed clones which have the following designations ug092 ft (SEQ ID NO:744); ug092ors (SEQ ID NO: 745); ug093f (SEQ ID NO:746); ug093ft (SEQ ID NO:747); ug106ft (SEQ ID NO:748); ug106ors (SEQ ID NO:749); ug120fmin (SEQ ID NO:750); ug120os (SEQ ID NO:751); ug254f (SEQ ID NO:752); ug254ors (SEQ ID NO:753); ug277f (SEQ ID NO:754); ug277ors (SEQ ID NO:755); ug277t (SEQ ID NO:756); ug291ft (SEQ ID NO:757); ug291ors (SEQ ID NO:758); ug307cons (SEQ ID NO:759); ug308f (SEQ ID NO:760); ug308o (SEQ ID NO: 761); ug308t (SEQ ID NO:762); ug311cons (SEQ ID NO:763); ug316cons (SEQ ID NO: 764); ug317cons (SEQ ID NO:765); ug320f (SEQ ID NO:766); ug320ors (SEQ ID NO:767); ug334ft (SEQ ID NO:768); ug334ors (SEQ ID NO:769); ug335ors (SEQ ID NO:770); ug335t (SEQ ID NO:771); ug353ft (SEQ ID NO:772); ug353ors (SEQ ID NO:773); ug354cons (SEQ ID NO:774); ug357ft (SEQ ID NO:775); ug357ors (SEQ ID NO:776); ug375cons (SEQ ID NO:777); ug371f (SEQ ID NO:778); ug440f (SEQ ID NO:779); ug440rs (SEQ ID NO:780); ug441ft (SEQ ID NO:781); ug441ors (SEQ ID NO:782); ug482ft (SEQ ID NO:783); ug093ors (SEQ ID NO:784); ug096f (SEQ ID NO:785); ug096ors (SEQ ID NO:786); ug101orsft (SEQ ID NO:787); ug102cons (SEQ ID NO:788); ug482ors (SEQ ID NO:789); ug484ft (SEQ ID NO:790); ug484ors (SEQ ID NO:791); ug485ors (SEQ ID NO:792); ug485t (SEQ ID NO:793); ug491ft (SEQ ID NO:794); ug491ors (SEQ ID NO:795); ug493ft (SEQ ID NO:796); ug493ors (SEQ ID NO:797); ug494cons (SEQ ID NO:798); ug503ft (SEQ ID NO:799); ug503r (SEQ ID NO:800); ug503s (SEQ ID NO:801); ug505ft (SEQ ID NO:802); ug505ors (SEQ ID NO:803); ug506ft (SEQ ID NO:804); ug506or (SEQ ID NO:805); ugs148oft (SEQ ID NO:806); ugs148rs (SEQ ID NO:807); ugs186oft (SEQ ID NO:808); ugs186s (SEQ ID NO:809); ugs194oft (SEQ ID NO:810); ugs194rs (SEQ ID NO:811).

[0064] Accordingly, the present invention relates to methods and compositions for the treatment and diagnosis of prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland. Specifically, fetal genes are identified and described which are differentially expressed in prostate disease states, relative to their expression in normal, or non-prostate disease states.

[0065] The present invention further relates to screening methods to identify compositions and their therapeutic use for the treatment of prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland. “Differential expression”, as used herein, refers to both quantitative as well as qualitative differences in the fetal genes' temporal and/or tissue expression patterns. Differentially expressed fetal genes may represent “fingerprint genes,” and/or “target genes.” “Fingerprint gene,” as used herein, refers to a differentially expressed fetal gene whose expression pattern may be utilized as part of a prognostic or diagnostic for prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland, disease evaluation, or which, alternatively, may be used in methods for identifying compounds useful for the treatment of prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland. “Target gene”, as used herein, refers to a differentially expressed gene involved in prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland such that modulation of the level of target gene expression or of target gene product activity may act to ameliorate a prostate disease condition. Compounds that modulate target gene expression or activity of the target gene product can be used in the treatment of prostate disease.

[0066] Further, “pathway genes” are defined via the ability of their products to interact with other gene products involved in the development of prostate disease, or the progression of prostate disease. Pathway genes may also exhibit target gene and/or fingerprint gene characteristics. Although the genes described herein may be differentially expressed with respect to prostate disease, and/or their products may interact with gene products important to prostate disease, the genes may also be involved in mechanisms important to additional prostate processes.

[0067] The invention further includes the products of such fingerprint, target, and pathway genes, as well as antibodies to such gene products. Furthermore, the engineering and use of cell- and animal-based models of prostate disease to which such gene products may contribute are also described.

[0068] The present invention encompasses methods for prognostic and diagnostic evaluation of prostate disease conditions, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland, and for the identification of subjects exhibiting a predisposition to such conditions. Furthermore, the invention provides methods for evaluating the efficacy of drugs, and monitoring the progress of patients, involved in clinical trials for the treatment of prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland.

[0069] The invention also provides methods for the identification of compounds that modulate the expression of genes or the activity of gene products involved in prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland as well as methods for the treatment of prostate disease which may involve the administration of such compounds to individuals exhibiting prostate disease symptoms or tendencies.

[0070] The invention also provides methods for the identification of compounds that modulate the expression of genes or the activity of gene products involved in prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland.

[0071] The invention is based, in part, on systematic search strategies involving in vivo and in vitro prostate disease models, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland, coupled with sensitive and high throughput gene expression assays. In contrast to approaches that merely evaluate the expression of a given gene product presumed to play a role in a prostate disease process, the search strategies and assays used herein permit the identification of all genes, whether known or novel, that are expressed or repressed in the prostate disease condition, as well as the evaluation of their temporal regulation and function during prostate disease progression. This comprehensive approach and evaluation permits the discovery of novel genes and gene products, as well as the identification of an array of genes and gene products (whether novel or known) involved in novel pathways that play a major role in prostate disease pathology. Thus, the invention allows one to define targets useful for diagnosis, monitoring, rational drug screening and design, and/or other therapeutic intervention for prostatic disease processes, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland.

[0072] In the working examples described herein, novel human genes are identified that are demonstrated to be differentially expressed in different prostate disease states. The identification of these genes and the characterization of their expression in particular prostate disease states provide newly identified roles in prostate disease for these genes.

[0073] Specifically, ug311, and ug494 are two novel fetal urogenital sinus (UGS)-derived expressed sequence tags (ESTs) which represent novel genes that are each differentially regulated in the LNCaP progression prostate cancer model. The fetal gene-derived EST Ug311 is down-regulated in the aggressive, androgen independent PCa cell line, C4-2, whereas the fetal gene-derived EST ug494 is up-regulated in the C4-2 cell line compared to the LNCaP progression prostate cancer model cell line. The isolation and characterization of the fetal gene-derived EST Ug311 is presented in more detail in Example 1.

[0074] Accordingly, methods are provided for the diagnosis, monitoring in clinical trials, screening for therapeutically effective compounds, and treatment of prostate disease, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland based upon the discoveries herein regarding the expression patterns of the fetal UGS-derived ESTs, ug311 and ug494.

[0075] The characteristic up-regulation of the ug494 fetal gene can be used to design prostate disease treatment strategies. For those up-regulated fetal genes that have a causative effect on the disease conditions, treatment methods can be designed to reduce or eliminate their expression, particularly in prostate cells. Alternatively, treatment methods include inhibiting the activity of the protein products of these fetal genes. For those up-regulated fetal genes that have a protective effect, treatment methods can be designed for enhancing the activity of the products of such fetal genes.

[0076] In either situation, detecting expression of these genes in excess of normal expression provides for the diagnosis of prostate disease. Furthermore, in testing the efficacy of compounds during clinical trials, a decrease in the level of the expression of these genes corresponds to a return from a disease condition to a normal state, and thereby indicates a positive effect of the compound. The prostate diseases that may be so diagnosed, monitored in clinical trials, and treated include, but are not limited to, prostatitis, and benign and malignant growth of the prostate gland.

[0077] The characteristic down-regulation of the ug311 fetal gene can also be used to design prostate disease treatment strategies. For those genes whose down-regulation has a pathogenic effect, treatment methods can be designed to restore or increase their expression, particularly in prostate cells. Alternatively, treatment methods include increasing the activity of the protein products of these fetal genes. For those fetal genes whose down-regulation has a protective effect, treatment methods can be designed for decreasing the amount or activity of the products of such fetal genes.

[0078] The invention encompasses methods for screening compounds and other substances for treating prostate disease symptoms, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland, by assaying the ability of such compounds and other substances to modulate the expression of either the ug311 or ug494 fetal UGS-derived EST genes disclosed herein or activity of the protein products of the ug311 or ug494 fetal UGS-derived EST genes. The invention further encompasses methods for screening compounds and other substances such as steroids, anti-steroids, chemotherapeutics, including, for example, without limitation, compounds or analogs for nucleotide metabolism or nucleotide synthesis, radiation sensitizing agents, DNA repair enzymes or drugs targeting DNA repair, including, for example, without limitation, DNA topoisomerase inhibitors, potential Ku inhibitors or interacting proteins, and differentiation compounds, including, for example, without limitation, phenylacetate, and phenylbutyrate, and derivatives of such compounds, which may be used for treating human prostatic diseases and syndromes including, without limitation, prostatitis, and benign and malignant growth of the prostate gland, by assaying the ability of such compounds and other substances to modulate the expression of the target fetal genes disclosed herein or activity of the protein products of the target fetal genes. Such screening methods include, but are not limited to, assays for identifying compounds and other substances that interact with (e.g., bind to) the either the ug311 or ug494 fetal UGS-derived ESTs fetal gene products disclosed herein.

[0079] The data presented in Example 1, below, demonstrates the use of the prostate disease model of the invention to identify prostate disease target fetal genes.

[0080] In either situation, detecting expression of these fetal genes in below normal expression provides for the diagnosis of prostate disease. Furthermore, in testing the efficacy of compounds during clinical trials, an increase in the level of the expression of these fetal genes corresponds to a return from a disease condition to a normal state, and thereby indicates a positive effect of the compound. The prostate diseases that may be so diagnosed, monitored in clinical trials, and treated include, but are not limited to, prostatitis, and benign and malignant growth of the prostate gland.

[0081] In addition, the invention encompasses methods for treating prostate disease by administering compounds and other substances that modulate the overall activity of the target fetal gene products. Compounds and other substances can effect such modulation either on the level of target gene expression or target protein activity.

[0082] In order to identify differentially expressed genes, RNA, either total or mRNA, may be isolated from one or more tissues of the subjects utilized in the model systems such as those described earlier in this Section. RNA samples are obtained from tissues of experimental subjects and from corresponding tissues of control subjects. Any RNA isolation technique which does not select against the isolation of mRNA may be utilized for the purification of such RNA samples. See, for example, Sambrook et al., 1989, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press, N.Y.; and Ausubel, F. M. et al., eds., 1987-1993, Current Protocols in Molecular Biology, John Wiley & Sons, Inc. New York, both of which are incorporated herein by reference in their entirety. Additionally, large numbers of tissue samples may readily be processed using techniques well known to those of skill in the art, such as, for example, the single-step RNA isolation process of Chomczynski, P. (1989, U.S. Pat. No. 4,843,155), which is incorporated herein by reference in its entirety.

[0083] Transcripts within the collected RNA samples which represent RNA produced by differentially expressed genes may be identified by utilizing a variety of methods which are well known to those of skill in the art. For example, differential screening (Tedder, T. F. et al., 1988, Proc. Natl. Acad. Sci. USA 85:208-212), subtractive hybridization (Hedrick, S. M. et al., 1984, Nature 308:149-153; Lee, S. W. et al., 1984, Proc. Natl. Acad. Sci. USA 88:2825), and, preferably, differential display (Liang, P., and Pardee, A. B., 1993, U.S. Patent No. 5,262,311, which is incorporated herein by reference in its entirety), may be utilized to identify nucleic acid sequences derived from genes that are differentially expressed.

[0084] Differential screening involves the duplicate screening of a cDNA library in which one copy of the library is screened with a total cell cDNA probe corresponding to the mRNA population of one cell type while a duplicate copy of the cDNA library is screened with a total cDNA probe corresponding to the mRNA population of a second cell type. For example, one cDNA probe may correspond to a total cell cDNA probe of a cell type derived from a control subject, while the second cDNA probe may correspond to a total cell cDNA probe of the same cell type derived from an experimental subject. Those clones which hybridize to one probe but not to the other potentially represent clones derived from genes differentially expressed in the cell type of interest in control versus experimental subjects.

[0085] Subtractive hybridization techniques generally involve the isolation of mRNA taken from two different sources, e.g., control and experimental tissue, the hybridization of the mRNA or single-stranded cDNA reverse-transcribed from the isolated mRNA, and the removal of all hybridized, and therefore double-stranded, sequences. The remaining non-hybridized, single-stranded cDNAs, potentially represent clones derived from genes that are differentially expressed in the two mRNA sources. Such single-stranded cDNAs are then used as the starting material for the construction of a library comprising clones derived from differentially expressed genes.

[0086] The differential display technique describes a procedure, utilizing the well known polymerase chain reaction (PCR; the experimental embodiment set forth in Mullis, K. B., 1987, U.S. Pat. No. 4,683,202) which allows for the identification of sequences derived from genes which are differentially expressed. First, isolated RNA is reverse-transcribed into single-stranded cDNA, utilizing standard techniques which are well known to those of skill in the art. Primers for the reverse transcriptase reaction may include, but are not limited to, oligo dT-containing primers, preferably of the reverse primer type of oligonucleotide described below. Next, this technique uses pairs of PCR primers, as described below, which allow for the amplification of clones representing a random subset of the RNA transcripts present within any given cell. Utilizing different pairs of primers allows each of the mRNA transcripts present in a cell to be amplified. Among such amplified transcripts may be identified those which have been produced from differentially expressed genes.

[0087] The reverse oligonucleotide primer of the primer pairs may contain an oligo dT stretch of nucleotides, preferably eleven nucleotides long, at its 5′ end, which hybridizes to the poly(A) tail of mRNA or to the complement of a cDNA reverse transcribed from an mRNA poly(A) tail. Second, in order to increase the specificity of the reverse primer, the primer may contain one or more, preferably two, additional nucleotides at its 3′ end. Because, statistically, only a subset of the mRNA derived sequences present in the sample of interest will hybridize to such primers, the additional nucleotides allow the primers to amplify only a subset of the mRNA derived sequences present in the sample of interest. This is preferred in that it allows more accurate and complete visualization and characterization of each of the bands representing amplified sequences.

[0088] The forward primer may contain a nucleotide sequence expected, statistically, to have the ability to hybridize to cDNA sequences derived from the tissues of interest. The nucleotide sequence may be an arbitrary one, and the length of the forward oligonucleotide primer may range from about 9 to about 13 nucleotides, with about 10 nucleotides being preferred. Arbitrary primer sequences cause the lengths of the amplified partial cDNAs produced to be variable, thus allowing different clones to be separated by using standard denaturing sequencing gel electrophoresis. PCR reaction conditions should be chosen which optimize amplified product yield and specificity, and, additionally, produce amplified products of lengths which may be resolved utilizing standard gel electrophoresis techniques. Such reaction conditions are well known to those of skill in the art, and important reaction parameters include, for example, length and nucleotide sequence of oligonucleotide primers as discussed above, and annealing and elongation step temperatures and reaction times.

[0089] The pattern of clones resulting from the reverse transcription and amplification of the mRNA of two different cell types is displayed via sequencing gel electrophoresis and compared. Differences in the two banding patterns indicate potentially differentially expressed genes.

[0090] Once potentially differentially expressed gene sequences have been identified via bulk techniques such as, for example, those described above, the differential expression of such putatively differentially expressed genes should be corroborated. Corroboration may be accomplished via, for example, such well known techniques as Northern analysis and/or RT-PCR.

[0091] Also, amplified sequences of differentially expressed genes obtained through, for example, differential display may be used to isolate full length clones of the corresponding gene. The full length coding portion of the gene may readily be isolated, without undue experimentation, by molecular biological techniques well known in the art. For example, the isolated differentially expressed amplified fragment may be labeled and used to screen a cDNA library. Alternatively, the labeled fragment may be used to screen a genomic library.

[0092] PCR technology may also be utilized to isolate full length cDNA sequences. As described above, the isolated, amplified gene fragments obtained through differential display have 5′ terminal ends at some random point within the gene and have 3′ terminal ends at a position preferably corresponding to the 3′ end of the transcribed portion of the gene. Once nucleotide sequence information from an amplified fragment is obtained, the remainder of the gene (i.e., the 5′ end of the gene, when utilizing differential display) may be obtained using, for example, RT-PCR.

[0093] In one embodiment of such a procedure for the identification and cloning of full length gene sequences, RNA may be isolated, following standard procedures, from an appropriate tissue or cellular source. A reverse transcription reaction may then be performed on the RNA using an oligonucleotide primer complimentary to the mRNA that corresponds to the amplified fragment, for the priming of first strand synthesis. Because the primer is anti-parallel to the mRNA, extension will proceed toward the 5′ end of the mRNA. The resulting RNA/DNA hybrid may then be “tailed” with guanines using a standard terminal transferase reaction, the hybrid may be digested with RNAase H, and second strand synthesis may then be primed with a poly-C primer. Using the two primers, the 5′ portion of the gene is amplified using PCR. Sequences obtained may then be isolated and recombined with previously isolated sequences to generate a full-length cDNA of the differentially expressed genes of the invention. For a review of cloning strategies and recombinant DNA techniques, see s, Sambrook et al., 1989, supra; and Ausubel et al., 1989, supra.

[0094] As used herein, “differentially expressed gene” (i.e. target and fingerprint gene) or “pathway gene” refers to (a) a gene containing at least one of the DNA sequences disclosed herein (as shown in FIG. 1 and FIG. 9), or contained in the UGS-derived ESTs listed in Tables 1-6; (b) any DNA sequence that encodes the amino acid sequence encoded by the DNA sequences disclosed herein (as shown in FIG. 1 and FIG. 9), contained in the ESTs listed in Tables 1-6, or contained within the coding region of the gene to which the DNA sequences disclosed herein (as shown in FIG. 1 and FIG. 9) or contained in the ESTs listed in Tables 1-6, belong; (c) any DNA sequence that hybridizes to the complement of the coding sequences disclosed herein (as shown in FIG. 1 and FIG. 9), contained in the ESTs listed in Tables 1-6, or contained within the coding region of the gene to which the DNA sequences disclosed herein (as shown in FIG. 1 and FIG. 9) or contained in the ESTs listed in Tables 1-6, under highly stringent conditions, e.g., hybridization to filter-bound DNA in 0.5 M NaHPO₄, 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at 65° C., and washing in 0.1×SSC/0.1% SDS at 68° C. (Ausubel F. M. et al., eds., 1989, Current Protocols in Molecular Biology, Vol. I, Green Publishing Associates, Inc., and John Wiley & sons, Inc., New York, at p. 2.10.3) and encodes a fetal gene product functionally equivalent to a gene product encoded by the DNA sequences disclosed herein (as shown in FIG. 1 and FIG. 9) or a gene product encoded by sequences contained within the ESTs listed in Tables 1-6; and/or (d) any DNA sequence that hybridizes to the complement of the coding sequences disclosed herein, (as shown in FIG. 1 and FIG. 9) contained in the ESTs listed in Tables 1-6, or contained within the coding region of the gene to which DNA sequences disclosed herein (as shown in FIG. 1 and FIG. 9) or contained in the ESTs, listed in Tables 1-6, belong, under less stringent conditions, such as moderately stringent conditions, e.g., washing in 0.2×SSC/0.1% SDS at 42° C. (Ausubel et al., 1989, supra), yet which still encodes a functionally equivalent fetal gene product.

[0095] The invention also includes nucleic acid molecules, preferably DNA molecules, that hybridize to, and are therefore the complements of, the DNA sequences (a) through (c), in the preceding paragraph. Such hybridization conditions may be highly stringent or less highly stringent, as described above. In instances wherein the nucleic acid molecules are deoxyoligonucleotides (“oligos”), highly stringent conditions may refer, e.g., to washing in 6×SSC/0.05% sodium pyrophosphate at 37° C. (for 14-base oligos), 48° C. (for 17-base oligos), 55° C. (for 20-base oligos), and 60° C. (for 23-base oligos). These nucleic acid molecules may act as target gene antisense molecules, useful, for example, in target gene regulation and/or as antisense primers in amplification reactions of target gene nucleic acid sequences. Further, such sequences may be used as part of ribozyme and/or triple helix sequences, which are also useful for target gene regulation. Still further, such molecules may be used as components of diagnostic methods whereby the presence of a prostate disease-causing allele, may be detected.

[0096] The nucleotide sequences of the invention also include nucleotide sequences that have at least 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, or more nucleotide sequence identity to a gene containing at least one of the DNA sequences disclosed herein (as shown in FIG. 1 and FIG. 9). The nucleotide sequences of the invention further include nucleotide sequences that encode polypeptides having at least 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, or higher amino acid sequence identity to the polypeptides encoded by the nucleotide sequences disclosed herein (as shown in FIG. 1 and FIG. 9).

[0097] To determine the percent identity of two amino acid sequences or of two nucleic acids, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of a first amino acid or nucleic acid sequence for optimal alignment with a second amino or nucleic acid sequence). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity=# of identical overlapping positions/total # of positions×100%). In one embodiment, the two sequences are the same length.

[0098] The determination of percent identity between two sequences can also be accomplished using a mathematical algorithm. A preferred, non-limiting example of a mathematical algorithm utilized for the comparison of two sequences is the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA 87:2264-2268, modified as in Karlin and Altschul (1993)Proc. Natl. Acad. Sci. USA 90:5873-5877. Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul, et al. (1990) J. Mol. Biol. 215:403-410. BLAST nucleotide searches can be performed with the NBLAST program, score=100, wordlength=12 to obtain nucleotide sequences homologous to a nucleic acid molecules of the invention. BLAST protein searches can be performed with the XBLAST program, score=50, wordlength=3 to obtain amino acid sequences homologous to a protein molecules of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al. (1997) Nucleic Acids Res.25:3389-3402. Alternatively, PSI-Blast can be used to perform an iterated search which detects distant relationships between molecules (Id). When utilizing BLAST, Gapped BLAST, and PSI-Blast programs, the default parameters of the respective programs (e.g., XBLAST and NBLAST) can be used (see http://www.ncbi.nlm.nih.gov). Another preferred, non-limiting example of a mathematical algorithm utilized for the comparison of sequences is the algorithm of Myers and Miller, (1988) CABIOS 4:11-17. Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used.

[0099] The percent identity between two sequences can be determined using techniques similar to those described above, with or without allowing gaps. In calculating percent identity, typically only exact matches are counted.

[0100] The invention also encompasses (a) DNA vectors that contain any of the foregoing coding sequences and/or their complements (i.e., antisense); (b) DNA expression vectors that contain any of the foregoing coding sequences operatively associated with a regulatory element that directs the expression of the coding sequences; and (c) genetically engineered host cells that contain any of the foregoing coding sequences operatively associated with a regulatory element that directs the expression of the coding sequences in the host cell. As used herein, regulatory elements include but are not limited to inducible and non-inducible promoters, enhancers, operators and other elements known to those skilled in the art that drive and regulate expression. The invention includes fragments of any of the DNA sequences disclosed herein.

[0101] In addition to the gene sequences described above, homologues of such sequences, as may, for example be present in other species, may be identified and may be readily isolated, without undue experimentation, by molecular biological techniques well known in the art. Further, there may exist genes at other genetic loci within the genome that encode proteins which have extensive homology to one or more domains of such gene products. These genes may also be identified via similar techniques.

[0102] For example, the isolated differentially expressed gene sequence may be labeled and used to screen a cDNA library constructed from mRNA obtained from the organism of interest. Hybridization conditions will be of a lower stringency when the cDNA library was derived from an organism different from the type of organism from which the labeled sequence was derived. Alternatively, the labeled fragment may be used to screen a genomic library derived from the organism of interest, again, using appropriately stringent conditions. Such low stringency conditions will be well known to those of skill in the art, and will vary predictably depending on the specific organisms from which the library and the labeled sequences are derived. For guidance regarding such conditions see, for example, Sambrook et al., 1989, Molecular Cloning, A Laboratory Manual, Cold Springs Harbor Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y.

[0103] Further, a previously unknown differentially expressed or pathway gene-type sequence may be isolated by performing PCR using two degenerate oligonucleotide primer pools designed on the basis of amino acid sequences within the gene of interest. The template for the reaction may be cDNA obtained by reverse transcription of mRNA prepared from human or non-human cell lines or tissue known or suspected to express a differentially expressed or pathway gene allele.

[0104] The PCR product may be subcloned and sequenced to insure that the amplified sequences represent the sequences of a differentially expressed or pathway gene-like nucleic acid sequence. The PCR fragment may then be used to isolate a full length cDNA clone by a variety of methods. For example, the amplified fragment may be labeled and used to screen a bacteriophage cDNA library. Alternatively, the labeled fragment may be used to screen a genomic library.

[0105] PCR technology may also be utilized to isolate full length cDNA sequences. For example, RNA may be isolated, following standard procedures, from an appropriate cellular or tissue source. A reverse transcription reaction may be performed on the RNA using an oligonucleotide primer specific for the most 5′ end of the amplified fragment for the priming of first strand synthesis. The resulting RNA/DNA hybrid may then be “tailed” with guanines using a standard terminal transferase reaction, the hybrid may be digested with RNAase H, and second strand synthesis may then be primed with a poly-C primer. Thus, cDNA sequences upstream of the amplified fragment may easily be isolated. For a review of cloning strategies which may be used, see e.g., Sambrook et al., 1989, supra.

[0106] In cases where the differentially expressed or pathway gene identified is the normal, or wild type, gene, this gene may be used to isolate mutant alleles of the gene. Such an isolation is preferable in processes and disorders which are known or suspected to have a genetic basis. Mutant alleles may be isolated from individuals either known or suspected to have a genotype which contributes to prostate disease symptoms. Mutant alleles and mutant allele products may then be utilized in the therapeutic and diagnostic assay systems described below.

[0107] A cDNA of the mutant gene may be isolated, for example, by using PCR, a technique which is well known to those of skill in the art. In this case, the first cDNA strand may be synthesized by hybridizing an oligo-dT oligonucleotide to mRNA isolated from tissue known or suspected to be expressed in an individual putatively carrying the mutant allele, and by extending the new strand with reverse transcriptase. The second strand of the cDNA is then synthesized using an oligonucleotide that hybridizes specifically to the 5′ end of the normal gene. Using these two primers, the product is then amplified via PCR, cloned into a suitable vector, and subjected to DNA sequence analysis through methods well known to those of skill in the art. By comparing the DNA sequence of the mutant gene to that of the normal gene, the mutation(s) responsible for the loss or alteration of function of the mutant gene product can be ascertained.

[0108] Alternatively, a genomic or cDNA library can be constructed and screened using DNA or RNA, respectively, from a tissue known to or suspected of expressing the gene of interest in an individual suspected of or known to carry the mutant allele. The normal gene or any suitable fragment thereof may then be labeled and used as a probed to identify the corresponding mutant allele in the library. The clone containing this gene may then be purified through methods routinely practiced in the art, and subjected to sequence analysis as described above.

[0109] Additionally, an expression library can be constructed utilizing DNA isolated from or cDNA synthesized from a tissue known to or suspected of expressing the gene of interest in an individual suspected of or known to carry the mutant allele. In this manner, gene products made by the putatively mutant tissue may be expressed and screened using standard antibody screening techniques in conjunction with antibodies raised against the normal gene product, as described below. (For screening techniques, see, for example, Harlow, E. and Lane, eds., 1988, “Antibodies: A Laboratory Manual”, Cold Spring Harbor Press, Cold Spring Harbor.) In cases where the mutation results in an expressed gene product with altered function (e.g., as a result of a missense mutation), a polyclonal set of antibodies are likely to cross-react with the mutant gene product. Library clones detected via their reaction with such labeled antibodies can be purified and subjected to sequence analysis as described above.

[0110] In addition, differentially expressed and pathway gene products may include proteins that represent functionally equivalent gene products. Such an equivalent differentially expressed or pathway gene product may contain deletions, additions or substitutions of amino acid residues within the amino acid sequence encoded by the differentially expressed or pathway gene sequences described above but which result in a silent change, thus producing a functionally equivalent differentially expressed on pathway gene product. Amino acid substitutions may be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues involved.

[0111] For example, nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; positively charged (basic) amino acids include arginine, lysine, and histidine; and negatively charged (acidic) amino acids include aspartic acid and glutamic acid. “Functionally equivalent”, as utilized herein, refers to a protein capable of exhibiting a substantially similar in vivo activity as the endogenous differentially expressed or pathway gene products encoded by the differentially expressed or pathway gene sequences described above. Alternatively, when utilized as part of assays such as those described below, “functionally equivalent” may refer to peptides capable of interacting with other cellular or extracellular molecules in a manner substantially similar to the way in which the corresponding portion of the endogenous differentially expressed or pathway gene product would.

[0112] The differentially expressed or pathway gene products may be produced by recombinant DNA technology using techniques well known in the art. Thus, methods for preparing the differentially expressed or pathway gene polypeptides and peptides of the invention by expressing nucleic acid encoding differentially expressed or pathway gene sequences are described herein. Methods which are well known to those skilled in the art can be used to construct expression vectors containing differentially expressed or pathway gene protein coding sequences and appropriate transcriptional/translational control signals. These methods include, for example, in vitro recombinant DNA techniques, synthetic techniques and in vivo recombination/genetic recombination. See, for example, the techniques described in Sambrook et al., 1989, supra, and Ausubel et al., 1989, supra. Alternatively, RNA capable of encoding differentially expressed or pathway gene protein sequences may be chemically synthesized using, for example, synthesizers. See, for example, the techniques described in “Oligonucleotide Synthesis”, 1984, Gait, M. J. ed., IRL Press, Oxford, which is incorporated by reference herein in its entirety.

[0113] Vectors, Host Cells, and Recombinant Expression

[0114] A variety of host-expression vector systems may be utilized to express the differentially expressed or pathway gene coding sequences of the invention. Such host-expression systems represent vehicles by which the coding sequences of interest may be produced and subsequently purified, but also represent cells which may, when transformed or transfected with the appropriate nucleotide coding sequences, exhibit the differentially expressed or pathway gene protein of the invention in situ. These include but are not limited to microorganisms such as bacteria (e.g., E. coli, B. subtilis) transformed with recombinant bacteriophage DNA, plasmid DNA or cosmid DNA expression vectors containing differentially expressed or pathway gene protein coding sequences; yeast (e.g. Saccharomyces, Pichia) transformed with recombinant yeast expression vectors containing the differentially expressed or pathway gene protein coding sequences; insect cell systems infected with recombinant virus expression vectors (e.g., baculovirus) containing the differentially expressed or pathway gene protein coding sequences; plant cell systems infected with recombinant virus expression vectors (e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or transformed with recombinant plasmid expression vectors (e.g., Ti plasmid) containing differentially expressed or pathway gene protein coding sequences; or mammalian cell systems (e.g. COS, CHO, BHK, 293, 3T3) harboring recombinant expression constructs containing promoters derived from the genome of mammalian cells (e.g., metallothionein promoter) or from mammalian viruses (e.g., the adenovirus late promoter; the vaccinia virus 7.5K promoter).

[0115] In bacterial systems, a number of expression vectors may be advantageously selected depending upon the use intended for the differentially expressed or pathway gene protein being expressed. For example, when a large quantity of such a protein is to be produced, for the generation of antibodies or to screen peptide libraries, for example, vectors which direct the expression of high levels of fusion protein products that are readily purified may be desirable. Such vectors include, but are not limited, to the E. coli expression vector pUR278 (Ruther et al., 1983, EMBO J. 2:1791), in which the differentially expressed or pathway gene protein coding sequence may be ligated individually into the vector in frame with the lac Z coding region so that a fusion protein is produced; pIN vectors (Inouye & Inouye, 1985, Nucleic Acids Res. 13:3101-3109; Van Heeke & Schuster, 1989, J. Biol. Chem. 264:5503-5509); and the like. pGEX vectors may also be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST). In general, such fusion proteins are soluble and can easily be purified from lysed cells by adsorption to glutathione-agarose beads followed by elution in the presence of free glutathione. The pGEX vectors are designed to include thrombin or factor Xa protease cleavage sites so that the cloned target gene protein can be released from the GST moiety.

[0116] In a preferred embodiment, full length cDNA sequences are appended with in-frame BamHI sites at the amino terminus and EcoRI sites at the carboxyl terminus using standard PCR methodologies (Innis et al., 1990, supra) and ligated into the pGEX-2TK vector (Pharmacia, Uppsala, Sweden). The resulting cDNA construct contains a kinase recognition site at the amino terminus for radioactive labelling and glutathione 5-transferase sequences at the carboxyl terminus for affinity purification (Nilsson, et al., 1985, EMBO J.4:1075; Zabeau and Stanley, 1982, EMBO J 1:1217.

[0117] In an insect system, Autographa californica nuclear polyhedrosis virus (AcNPV) is used as a vector to express foreign genes. The virus grows in Spodoptera frugiperda cells. The differentially expressed or pathway gene coding sequence may be cloned individually into non-essential regions (for example the polyhedrin gene) of the virus and placed under control of an AcNPV promoter (for example the polyhedrin promoter). Successful insertion of differentially expressed or pathway gene coding sequence will result in inactivation of the polyhedrin gene and production of non-occluded recombinant virus (i.e., virus lacking the proteinaceous coat coded for by the polyhedrin gene). These recombinant viruses are then used to infect Spodoptera frugiperda cells in which the inserted gene is expressed. (E.g., see Smith et al., 1983, J. Virol. 46:584; Smith, U.S. Pat. No. 4,215,051).

[0118] In mammalian host cells, a number of viral-based expression systems may be utilized. In cases where an adenovirus is used as an expression vector, the differentially expressed or pathway gene coding sequence of interest may be ligated to an adenovirus transcription/translation control complex, e.g., the late promoter and tripartite leader sequence. This chimeric gene may then be inserted in the adenovirus genome by in vitro or in vivo recombination. Insertion in a non-essential region of the viral genome (e.g., region E1 or E3) will result in a recombinant virus that is viable and capable of expressing differentially expressed or pathway gene protein in infected hosts. (E.g., See Logan & Shenk, 1984, Proc. Natl. Acad. Sci. USA 81:3655-3659). Specific initiation signals may also be required for efficient translation of inserted differentially expressed or pathway gene coding sequences. These signals include the ATG initiation codon and adjacent sequences. In cases where an entire differentially expressed or pathway gene, including its own initiation codon and adjacent sequences, is inserted into the appropriate expression vector, no additional translational control signals may be needed. However, in cases where only a portion of the differentially expressed or pathway gene coding sequence is inserted, exogenous translational control signals, including, perhaps, the ATG initiation codon, must be provided. Furthermore, the initiation codon must be in phase with the reading frame of the desired coding sequence to ensure translation of the entire insert. These exogenous translational control signals and initiation codons can be of a variety of origins, both natural and synthetic. The efficiency of expression may be enhanced by the inclusion of appropriate transcription enhancer elements, transcription terminators, etc. (see Bittner et al., 1987, Methods in Enzymol. 153:516-544).

[0119] In a preferred embodiment, cDNA sequences encoding the full-length open reading frames are ligated into pCMVβ replacing the β-galactosidase gene such that cDNA expression is driven by the CMV promoter (Alam, 1990, Anal. Biochem. 188:245-254; MacGregor & Caskey, 1989, Nucl. Acids Res. 17:2365; Norton & Corrin, 1985, Mol. Cell. Biol. 5:281).

[0120] In addition, a host cell strain may be chosen which modulates the expression of the inserted sequences, or modifies and processes the gene product in the specific fashion desired. Such modifications (e.g., glycosylation) and processing (e.g., cleavage) of protein products may be important for the function of the protein. Different host cells have characteristic and specific mechanisms for the post-translational processing and modification of proteins. Appropriate cell lines or host systems can be chosen to ensure the correct modification and processing of the foreign protein expressed. To this end, eukaryotic host cells which possess the cellular machinery for proper processing of the primary transcript, glycosylation, and phosphorylation of the gene product may be used. Such mammalian host cells include but are not limited to CHO, VERO, BHK, HeLa, COS, MDCK, 293, 3T3, W138, etc.

[0121] For long-term, high-yield production of recombinant proteins, stable expression is preferred. For example, cell lines which stably express the differentially expressed or pathway gene protein may be engineered. Rather than using expression vectors which contain viral origins of replication, host cells can be transformed with DNA controlled by appropriate expression control elements (e.g., promoter, enhancer, sequences, transcription terminators, polyadenylation sites, etc.), and a selectable marker. Following the introduction of the foreign DNA, engineered cells may be allowed to grow for 1-2 days in an enriched media, and then are switched to a selective media. The selectable marker in the recombinant plasmid confers resistance to the selection and allows cells to stably integrate the plasmid into their chromosomes and grow to form foci which in turn can be cloned and expanded into cell lines. This method may advantageously be used to engineer cell lines which express the differentially expressed or pathway gene protein. Such engineered cell lines may be particularly useful in screening and evaluation of compounds that affect the endogenous activity of the differentially expressed or pathway gene protein.

[0122] A number of selection systems may be used, including but not limited to the herpes simplex virus thymidine kinase (Wigler, et al., 1977, Cell 11:223), hypoxanthine-guanine phosphoribosyltransferase (Szybalski & Szybalski, 1962, Proc. Natl. Acad. Sci. USA 48:2026), and adenine phosphoribosyltransferase (Lowy, et al., 1980, Cell 22:817) genes can be employed in tk-, hgprt- or aprt cells, respectively. Also, antimetabolite resistance can be used as the basis of selection for dhfr, which confers resistance to methotrexate (Wigler, et al., 1980, Natl. Acad. Sci. USA 77:3567; O'Hare, et al., 1981, Proc. Natl. Acad. Sci. USA 78:1527); gpt, which confers resistance to mycophenolic acid (Mulligan & Berg, 1981, Proc. Natl. Acad. Sci. USA 78:2072); neo, which confers resistance to the aminoglycoside G418 (Colberre-Garapin, et al., 1981, J. Mol. Biol. 150:1); and hygro, which confers resistance to hygromycin (Santerre, et al., 1984, Gene 30:147) genes.

[0123] An alternative fusion protein system allows for the ready purification of non-denatured fusion proteins expressed in human cell lines (Janknecht, et al., 1991, Proc. Natl. Acad. Sci. USA 88:8972-8976). In this system, the gene of interest is subcloned into a vaccinia recombination plasmid such that the gene's open reading frame is translationally fused to an amino-terminal tag consisting of six histidine residues. Extracts from cells infected with recombinant vaccinia virus are loaded onto Ni²⁺ nitriloacetic acid-agarose columns and histidine-tagged proteins are selectively eluted with imidazole-containing buffers.

[0124] When used as a component in assay systems such as those described below, the differentially expressed or pathway gene protein may be labeled, either directly or indirectly, to facilitate detection of a complex formed between the differentially expressed or pathway gene protein and a test substance. Any of a variety of suitable labeling systems may be used including but not limited to radioisotopes such as ¹²⁵I; enzyme labeling systems that generate a detectable colorimetric signal or light when exposed to substrate; and fluorescent labels.

[0125] Where recombinant DNA technology is used to produce the differentially expressed or pathway gene protein for such assay systems, it may be advantageous to engineer fusion proteins that can facilitate labeling, immobilization and/or detection.

[0126] Indirect labeling involves the use of a protein, such as a labeled antibody, which specifically binds to either a differentially expressed or pathway gene product. Such antibodies include but are not limited to polyclonal, monoclonal, chimeric, single chain, Fab fragments and fragments produced by an Fab expression library.

[0127] Described herein are methods for the production of antibodies capable of specifically recognizing one or more differentially expressed or pathway gene epitopes. Such antibodies may include, but are not limited to polyclonal antibodies, monoclonal antibodies (mAbs), humanized or chimeric antibodies, single chain antibodies, Fab fragments, F(ab′)₂ fragments, fragments produced by a Fab expression library, anti-idiotypic (anti-Id) antibodies, and epitope-binding fragments of any of the above. Such antibodies may be used, for example, in the detection of a fingerprint, target, or pathway gene in a biological sample, or, alternatively, as a method for the inhibition of abnormal target gene activity. Thus, such antibodies may be utilized as part of prostate disease treatment methods, and/or may be used as part of diagnostic techniques whereby patients may be tested for abnormal levels of fingerprint, target, or pathway gene proteins, or for the presence of abnormal forms of such proteins.

[0128] For the production of antibodies to a differentially expressed or pathway gene, various host animals may be immunized by injection with a differentially expressed or pathway gene protein, or a portion thereof. Such host animals may include but are not limited to rabbits, mice, and rats, to name but a few. Various adjuvants may be used to increase the immunological response, depending on the host species, including but not limited to Freund's (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanin, dinitrophenol, and potentially useful human adjuvants such as BCG (bacille Calmette-Guerin) and Corynebacterium parvum.

[0129] In a preferred embodiment, peptide sequences corresponding to amino sequences of target gene products are selected and submitted for synthesis and antibody production. Peptides are modified as described (Tam, J. P., 1988, Proc. Natl. Acad. Sci. USA 85:5409-5413; Tam, J. P., and Zavala, F., 1989, J. Immunol. Methods 124:53-61; Tam, J. P., and Lu, Y. A., 1989, Proc. Natl. Acad. Sci. USA 86:9084-9088), emulsified in an equal volume of Freund's adjuvant and injected into rabbits at 3 to 4 subcutaneous dorsal sites for a total volume of 1.0 ml (0.5 mg peptide) per immunization. The animals are boosted after 2 and 6 weeks and bled at weeks 4, 8, and 10. The blood is allowed to clot and serum is collected by centrifugation. The generation of polyclonal antibodies against the ug311 EST-derived gene products is described in detail below.

[0130] Polyclonal antibodies are heterogeneous populations of antibody molecules derived from the sera of animals immunized with an antigen, such as target gene product, or an antigenic functional derivative thereof. For the production of polyclonal antibodies, host animals such as those described above, may be immunized by injection with differentially expressed or pathway gene product supplemented with adjuvants as also described above.

[0131] Monoclonal antibodies, which are homogeneous populations of antibodies to a particular antigen, may be obtained by any technique which provides for the production of antibody molecules by continuous cell lines in culture. These include, but are not limited to the hybridoma technique of Kohler and Milstein, (1975, Nature 256:495-497; and U.S. Pat. No. 4,376,110), the human B-cell hybridoma technique (Kosbor et al., 1983, Immunology Today 4:72; Cole et al., 1983, Proc. Natl. Acad. Sci. USA 80:2026-2030), and the EBV-hybridoma technique (Cole et al., 1985, Monoclonal Antibodies And Cancer Therapy, Alan R. Liss, Inc., pp.77-96). Such antibodies may be of any immunoglobulin class including IgG, IgM, IgE, IgA, IgD and any subclass thereof. The hybridoma producing the mAb of this invention may be cultivated in vitro or in vivo. Production of high titers of mAbs in vivo makes this the presently preferred method of production.

[0132] In addition, techniques developed for the production of “chimeric antibodies” (Morrison et al., 1984, Proc. Natl. Acad. Sci., 81:6851-6855; Neuberger et. al., 1984, Nature, 312:604-608; Takeda et al., 1985, Nature, 314:452-454) by splicing the genes from a mouse antibody molecule of appropriate antigen specificity together with genes from a human antibody molecule of appropriate biological activity can be used. A chimeric antibody is a molecule in which different portions are derived from different animal species, such as those having a variable region derived from a murine mAb and a human immunoglobulin constant region.

[0133] Alternatively, techniques described for the production of single chain antibodies (U.S. Pat. No. 4,946,778; Bird, 1988, Science 242:423-426; Huston et al., 1988, Proc. Natl. Acad. Sci. USA 85:5879-5883; and Ward et al., 1989, Nature 334:544-546) can be adapted to produce differentially expressed or pathway gene-single chain antibodies. Single chain antibodies are formed by linking the heavy and light chain fragments of the Fv region via an amino acid bridge, resulting in a single chain polypeptide.

[0134] Antibody fragments which recognize specific epitopes may be generated by known techniques. For example, such fragments include but are not limited to the F(ab′)₂ fragments which can be produced by pepsin digestion of the antibody molecule and the Fab fragments which can be generated by reducing the disulfide bridges of the F(ab′)₂ fragments. Alternatively, Fab expression libraries may be constructed (Huse et al., 1989, Science, 246:1275-1281) to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity.

[0135] Screening Assays for Compounds That Interact with the Target Gene Product and/or Modulate Target Gene Expression

[0136] The following assays are designed to identify compounds that bind to target gene products, bind to other cellular or extracellular proteins that interact with a target gene product, and interfere with the interaction of the target gene product with other cellular or extracellular proteins. Such compounds can act as the basis for amelioration of such prostate diseases, including, without limitation, prostatitis, and benign and malignant growth of the prostate gland by modulating the activity of the protein products of target genes. Such compounds may include, but are not limited to peptides, antibodies, or small organic or inorganic compounds. Such compounds may also include other cellular proteins. Methods for the identification of such cellular proteins are described below.

[0137] Compounds identified via assays such as those described herein may be useful, for example, in elaborating the biological function of the target gene product, and for ameliorating prostate disease including, without limitation, prostatitis, and benign and malignant growth of the prostate gland. In instances whereby a prostate disease condition results from an overall lower level of target gene expression and/or target gene product in a cell or tissue, compounds that interact with the target gene product may include compounds which accentuate or amplify the activity of the bound target gene protein. Such compounds would bring about an effective increase in the level of target gene product activity, thus ameliorating prostate disease symptoms.

[0138] In some cases, a target gene observed to be up-regulated under disease conditions may be exerting a protective effect. Compounds that enhance the expression of such up-regulated genes, or the activity of their gene products, would also ameliorate disease symptoms, especially in individuals whose target gene is not normally up-regulated.

[0139] In other instances mutations within the target gene may cause aberrant types or excessive amounts of target gene proteins to be made which have a deleterious effect that leads to prostate disease. Similarly, physiological conditions may cause an excessive increase in target gene expression leading to prostate disease. In such cases, compounds that bind target gene protein may be identified that inhibit the activity of the bound target gene protein. Assays for testing the effectiveness of compounds, identified by, for example, techniques such as those described above are discussed below.

[0140] In Vitro Screening Assays for Compounds That Bind to the Target Gene Product

[0141] In vitro systems may be designed to identify compounds capable of binding the target gene products of the invention. Such compounds may include, but are not limited to, peptides made of D-and/or L-configuration amino acids (in, for example, the form of random peptide libraries; see e.g., Lam, K. S. et al., 1991, Nature 354:82-84), phosphopeptides (in, for example, the form of random or partially degenerate, directed phosphopeptide libraries; see, e.g., Songyang, Z. et al., 1993, Cell 72:767-778), antibodies, and small organic or inorganic molecules. Compounds identified may be useful, for example, in modulating the activity of target gene proteins, preferably mutant target gene proteins, may be useful in elaborating the biological function of the target gene protein, may be utilized in screens for identifying compounds that disrupt normal target gene interactions, or may in themselves disrupt such interactions.

[0142] The principle of the assays used to identify compounds that bind to the target gene protein involves preparing a reaction mixture of the target gene protein and the test compound under conditions and for a time sufficient to allow the two components to interact and bind, thus forming a complex which can be removed and/or detected in the reaction mixture. These assays can be conducted in a variety of ways. For example, one method to conduct such an assay would involve anchoring the target gene or the test substance onto a solid phase and detecting target gene/test substance complexes anchored on the solid phase at the end of the reaction. In one embodiment of such a method, the target gene protein may be anchored onto a solid surface, and the test compound, which is not anchored, may be labeled, either directly or indirectly.

[0143] In practice, microtitre plates are conveniently utilized. The anchored component may be immobilized by non-covalent or covalent attachments. Non-covalent attachment may be accomplished simply by coating the solid surface with a solution of the protein and drying. Alternatively, an immobilized antibody, preferably a monoclonal antibody, specific for the protein may be used to anchor the protein to the solid surface. The surfaces may be prepared in advance and stored.

[0144] In order to conduct the assay, the non-immobilized component is added to the coated surface containing the anchored component. After the reaction is complete, unreacted components are removed (e.g., by washing) under conditions such that any complexes formed will remain immobilized on the solid surface. The detection of complexes anchored on the solid surface can be accomplished in a number of ways. Where the previously non-immobilized component is pre-labeled, the detection of label immobilized on the surface indicates that complexes were formed. Where the previously non-immobilized component is not pre-labeled, an indirect label can be used to detect complexes anchored on the surface; e.g., using a labeled antibody specific for the previously non-immobilized component (the antibody, in turn, may be directly labeled or indirectly labeled with a labeled anti-Ig antibody).

[0145] Alternatively, a reaction can be conducted in a liquid phase, the reaction products separated from unreacted components, and complexes detected; e.g., using an immobilized antibody specific for target gene product or the test compound to anchor any complexes formed in solution, and a labeled antibody specific for the other component of the possible complex to detect anchored complexes. Compounds such as those identified through assays described above which exhibit inhibitory activity may be used in accordance with the invention to ameliorate prostate disease symptoms. As discussed above, such molecules may include, but are not limited to small organic molecules, peptides, antibodies, and the like.

[0146] Pharmaceutical Preparations and Methods of Administration

[0147] The identified compounds that inhibit target gene expression, synthesis and/or activity can be administered to a patient at therapeutically effective doses to treat or ameliorate prostate disease, including, without limitation, prostatitis, and benign and malignant growth of the prostate gland. A therapeutically effective dose refers to that amount of the compound sufficient to result in amelioration of symptoms of prostate disease.

[0148] Effective Dose

[0149] Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g. for determining the LD₅₀ (the dose lethal to 50% of the population) and the ED₅₀ (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD₅₀/ED₅₀. Compounds which exhibit large therapeutic indices are preferred. While compounds that exhibit toxic side effects may be used, care should be taken to design a delivery system that targets such compounds to the site of affected tissue in order to minimize potential damage to uninfected cells and, thereby, reduce side effects.

[0150] The data obtained from the cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED₅₀ with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. For any compound used in the method of the invention, the therapeutically effective dose can be estimated initially from cell culture assays. A dose may be formulated in animal models to achieve a circulating plasma concentration range that includes the IC₅₀ i.e., the concentration of the test compound which achieves a half-maximal inhibition of symptoms) as determined in cell culture. Such information can be used to more accurately determine useful doses in humans. Levels in plasma may be measured, for example, by high performance liquid chromatography.

[0151] Formulations and Use

[0152] Pharmaceutical compositions for use in accordance with the present invention may be formulated in conventional manner using one or more physiologically acceptable carriers or excipients.

[0153] Thus, the compounds and their physiologically acceptable salts and solvates may be formulated for administration by inhalation or insufflation (either through the mouth or the nose) or oral, buccal, parenteral or rectal administration.

[0154] For oral administration, the pharmaceutical compositions may take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g., pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g., lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g., magnesium stearate, talc or silica); disintegrants (e.g., potato starch or sodium starch glycolate); or wetting agents (e.g., sodium lauryl sulphate). The tablets may be coated by methods well known in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g., methyl or propyl-p-hydroxybenzoates or sorbic acid). The preparations may also contain buffer salts, flavoring, coloring and sweetening agents as appropriate.

[0155] Preparations for oral administration may be suitably formulated to give controlled release of the active compound.

[0156] For buccal administration the compositions may take the form of tablets or lozenges formulated in conventional manner.

[0157] For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of e.g. gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.

[0158] The compounds may be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g., in ampoules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.

[0159] The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter or other glycerides.

[0160] In addition to the formulations described previously, the compounds may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.

[0161] The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack may for example comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration.

[0162] Diagnosis of Prostate Disease Abnormalities

[0163] A variety of methods may be employed, utilizing reagents such as fingerprint gene nucleotide sequences described above and antibodies directed against differentially expressed and pathway gene peptides, as described above. Specifically, such reagents may be used, for example, for the detection of the presence of target gene mutations, or the detection of either over or under expression of target gene mRNA.

[0164] The methods described herein may be performed, for example, by utilizing pre-packaged diagnostic kits comprising at least one specific fingerprint gene nucleic acid or anti-fingerprint gene antibody reagent described herein, which may be conveniently used, e.g., in clinical settings, to diagnose patients exhibiting prostate disease symptoms, including, without limitation, symptoms due to prostatitis, and benign and malignant growth of the prostate gland or at risk for developing prostate disease, including, without limitation, prostatitis, and benign and malignant growth of the prostate gland.

[0165] Any cell type or tissue, preferably prostate tissue, including, for example, without limitation, prostatic fibroblasts, prostatic epithelial cells, prostatic neuroendocrine cells and other cells of basal origin, endothelial cells, smooth muscle cells, osteoblastic lineages, osteoclastic lineages, and other transitional epithelial cells which include transitional epithelium of the bladder and kidney, in which the fingerprint gene is expressed may be utilized in the diagnostics described below.

[0166] Detection of Fingerprint Gene Nucleic Acids

[0167] DNA or RNA from the cell type or tissue to be analyzed may easily be isolated using procedures which are well known to those in the art. Diagnostic procedures may also be performed “in situ” directly upon tissue sections (fixed and/or frozen) of patient tissue obtained from biopsies or resections, such that no nucleic acid purification is necessary. Nucleic acid reagents such as those described above may be used as probes and/or primers for such in situ procedures (see, for example, Nuovo, G. J., 1992, PCR in situ hybridization:protocols and applications, Raven Press, N.Y.).

[0168] Fingerprint gene nucleotide sequences, either RNA or DNA, may, for example, be used in hybridization or amplification assays of biological samples to detect prostate disease-related gene structures and expression. Such assays may include, but are not limited to, Southern or Northern analyses, single stranded conformational polymorphism analyses, in situ hybridization assays, and polymerase chain reaction analyses. Such analyses may reveal both quantitative aspects of the expression pattern of the fingerprint gene, and qualitative aspects of the fingerprint gene expression and/or gene composition. That is, such aspects may include, for example, point mutations, insertions, deletions, chromosomal rearrangements, and/or activation or inactivation of gene expression.

[0169] Preferred diagnostic methods for the detection of fingerprint gene-specific nucleic acid molecules may involve for example, contacting and incubating nucleic acids, derived from the cell type or tissue being analyzed, with one or more labeled nucleic acid reagents as are described above, under conditions favorable for the specific annealing of these reagents to their complementary sequences within the nucleic acid molecule of interest. Preferably, the lengths of these nucleic acid reagents are at least 9 to 30 nucleotides. After incubation, all non-annealed nucleic acids are removed from the nucleic acid:fingerprint molecule hybrid. The presence of nucleic acids from the fingerprint tissue which have hybridized, if any such molecules exist, is then detected. Using such a detection scheme, the nucleic acid from the tissue or cell type of interest may be immobilized, for example, to a solid support such as a membrane, or a plastic surface such as that on a microtitre plate or polystyrene beads. In this case, after incubation, non-annealed, labeled fingerprint nucleic acid reagents of the type described above are easily removed. Detection of the remaining, annealed, labeled nucleic acid reagents is accomplished using standard techniques well-known to those in the art. Alternative diagnostic methods for the detection of fingerprint gene specific nucleic acid molecules may involve their amplification, e.g., by PCR (the experimental embodiment set forth in Mullis, K. B., 1987, U.S. Pat. No. 4,683,202), ligase chain reaction (Barany, F., 1991, Proc. Natl. Acad. Sci. USA 88:189-193), self sustained sequence replication (Guatelli, J. C. et al., 1990, Proc. Natl. Acad. Sci. USA 87:1874-1878), transcriptional amplification system (Kwoh, D. Y et al., 1989, Proc. Natl. Acad. Sci. USA 86:1173-1177), Q-Beta Replicase (Lizardi, P. M. et al., 1988, Bio/Technology 6:1197), or any other nucleic acid amplification method, followed by the detection of the amplified molecules using techniques well known to those of skill in the art. These detection schemes are especially useful for the detection of nucleic acid molecules if such molecules are present in very low numbers.

[0170] In one embodiment of such a detection scheme, a cDNA molecule is obtained from an RNA molecule of interest (e.g. by reverse transcription of the RNA molecule into cDNA). Cell types or tissues from which such RNA may be isolated include any tissue in which wild type fingerprint gene is known to be expressed, including, but not limited, to prostate tissue, endothelium, and/or smooth muscle. A fingerprint sequence within the cDNA is then used as the template for a nucleic acid amplification reaction, such as a PCR amplification reaction, or the like. The nucleic acid reagents used as synthesis initiation reagents (e.g., primers) in the reverse transcription and nucleic acid amplification steps of this method are chosen from among the fingerprint gene nucleic acid reagents described above. The preferred lengths of such nucleic acid reagents are at least 15-30 nucleotides. For detection of the amplified product, the nucleic acid amplification may be performed using radioactively or non-radioactively labeled nucleotides. Alternatively, enough amplified product may be made such that the product may be visualized by standard ethidium bromide staining or by utilizing any other suitable nucleic acid staining method.

[0171] In addition to methods which focus primarily on the detection of one nucleic acid sequence, fingerprint profiles may also be assessed in such detection schemes. Fingerprint profiles may be generated, for example, by utilizing a differential display procedure, Northern analysis and/or RT-PCR. Any of the gene sequences described above may be used as probes and/or PCR primers for the generation and corroboration of such fingerprint profiles.

[0172] Detection of Fingerprint Gene Peptides

[0173] Antibodies directed against wild type or mutant fingerprint gene peptides, which are discussed above may also be used as prostate disease diagnostics and prognostics, as described, for example, herein. Such diagnostic methods, may be used to detect abnormalities in the level of fingerprint gene protein expression, or abnormalities in the structure and/or tissue, cellular, or subcellular location of fingerprint gene protein. Structural differences may include, for example, differences in the size, electronegativity, or antigenicity of the mutant fingerprint gene protein relative to the normal fingerprint gene protein.

[0174] Protein from the prostate tissue or cell type to be analyzed may easily be detected or isolated using techniques which are well known to those of skill in the art, including but not limited to western blot analysis. For a detailed explanation of methods for carrying out western blot analysis, see Sambrook et al, 1989, supra, at Chapter 18. The protein detection and isolation methods employed herein may also be such as those described in Harlow and Lane, for example, (Harlow, E. and Lane, D., 1988, “Antibodies: A Laboratory Manual”, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.), which is incorporated herein by reference in its entirety.

[0175] Preferred diagnostic methods for the detection of wild type or mutant fingerprint gene peptide molecules may involve, for example, immunoassays wherein fingerprint gene peptides are detected by their interaction with an anti-fingerprint gene specific peptide antibody.

[0176] For example, antibodies, or fragments of antibodies, such as those described useful in the present invention may be used to quantitatively or qualitatively detect the presence of wild type or mutant fingerprint gene peptides. This can be accomplished, for example, by immunofluorescence techniques employing a fluorescently labeled antibody (see below) coupled with light microscopic, flow cytometric, or fluorimetric detection. Such techniques are especially preferred if the fingerprint gene peptides are expressed on the cell surface.

[0177] The antibodies (or fragments thereof) useful in the present invention may, additionally, be employed histologically, as in immunofluorescence or immunoelectron microscopy, for in situ detection of fingerprint gene peptides. In situ detection may be accomplished by removing a histological specimen from a patient, and applying thereto a labeled antibody of the present invention. The antibody (or fragment) is preferably applied by overlaying the labeled antibody (or fragment) onto a biological sample. Through the use of such a procedure, it is possible to determine not only the presence of the fingerprint gene peptides, but also their distribution in the examined tissue. Using the present invention, those of ordinary skill will readily perceive that any of a wide variety of histological methods (such as staining procedures) can be modified in order to achieve such in situ detection.

[0178] Immunoassays for wild type or mutant fingerprint gene peptides typically comprise incubating a biological sample, such as a biological fluid, a tissue extract, freshly harvested cells, or cells which have been incubated in tissue culture, in the presence of a detectably labeled antibody capable of identifying fingerprint gene peptides, and detecting the bound antibody by any of a number of techniques well known in the art.

[0179] The biological sample may be brought in contact with and immobilized onto a solid phase support or carrier such as nitrocellulose, or other solid support which is capable of immobilizing cells, cell particles or soluble proteins. The support may then be washed with suitable buffers followed by treatment with the detectably labeled fingerprint gene specific antibody. The solid phase support may then be washed with the buffer a second time to remove unbound antibody. The amount of bound label on solid support may then be detected by conventional means.

[0180] By “solid phase support or carrier” is intended any support capable of binding an antigen or an antibody. Well-known supports or carriers include glass, polystyrene, polypropylene, polyethylene, dextran, nylon, amylases, natural and modified celluloses, polyacrylamides, gabbros, and magnetite. The nature of the carrier can be either soluble to some extent or insoluble for the purposes of the present invention. The support material may have virtually any possible structural configuration so long as the coupled molecule is capable of binding to an antigen or antibody. Thus, the support configuration may be spherical, as in a bead, or cylindrical, as in the inside surface of a test tube, or the external surface of a rod. Alternatively, the surface may be flat such as a sheet, test strip, etc. Preferred supports include polystyrene beads. Those skilled in the art will know many other suitable carriers for binding antibody or antigen, or will be able to ascertain the same by use of routine experimentation.

[0181] The binding activity of a given lot of anti-wild type or mutant fingerprint gene peptide antibody may be determined according to well known methods. Those skilled in the art will be able to determine operative and optimal assay conditions for each determination by employing routine experimentation.

[0182] One of the ways in which the fingerprint gene peptide-specific antibody can be detectably labeled is by linking the same to an enzyme and use in an enzyme immunoassay (EIA) (Voller, “The Enzyme Linked Immunosorbent Assay (ELISA)”, Diagnostic Horizons 2:1-7, 1978, Microbiological Associates Quarterly Publication, Walkersville, Md.; Voller, et al., J. Clin. Pathol. 31:507-520 (1978); Butler, Meth. Enzymol. 73:482-523 (1981); Maggio, (ed.) Enzyme Immunoassay, CRC Press, Boca Raton, Fla., 1980; Ishikawa, et al., (eds.) Enzyme Immunoassay, Kgaku Shoin, Tokyo, 1981). The enzyme which is bound to the antibody will react with an appropriate substrate, preferably a chromogenic substrate, in such a manner as to produce a chemical moiety which can be detected, for example, by spectrophotometric, fluorimetric or by visual means. Enzymes which can be used to detectably label the antibody include, but are not limited to, malate dehydrogenase, staphylococcal nuclease, delta-5-steroid isomerase, yeast alcohol dehydrogenase, alpha-glycerophosphate, dehydrogenase, triose phosphate isomerase, horseradish peroxidase, alkaline phosphatase, asparaginase, glucose oxidase, beta-galactosidase, ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase, glucoamylase and acetylcholinesterase. The detection can be accomplished by colorimetric methods which employ a chromogenic substrate for the enzyme. Detection may also be accomplished by visual comparison of the extent of enzymatic reaction of a substrate in comparison with similarly prepared standards.

[0183] Detection may also be accomplished using any of a variety of other immunoassays. For example, by radioactively labeling the antibodies or antibody fragments, it is possible to detect fingerprint gene wild type or mutant peptides through the use of a radioimmunoassay (RIA) (see, for example, Weintraub, B., Principles of Radioimmunoassays, Seventh Training Course on Radioligand Assay Techniques, The Endocrine Society, March, 1986, which is incorporated by reference herein). The radioactive isotope can be detected by such means as the use of a gamma counter or a scintillation counter or by autoradiography.

[0184] It is also possible to label the antibody with a fluorescent compound. When the fluorescently labeled antibody is exposed to light of the proper wave length, its presence can then be detected due to fluorescence. Among the most commonly used fluorescent labeling compounds are fluorescein isothiocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde and fluorescamine.

[0185] The antibody can also be detectably labeled using fluorescence emitting metals such as ¹⁵²Eu, or others of the lanthanide series. These metals can be attached to the antibody using such metal chelating groups as diethylenetriaminepentacetic acid (DTPA) or ethylenediaminetetraacetic acid (EDTA).

[0186] The antibody also can be detectably labeled by coupling it to a chemiluminescent compound. The presence of the chemiluminescent-tagged antibody is then determined by detecting the presence of luminescence that arises during the course of a chemical reaction. Examples of particularly useful chemiluminescent labeling compounds are luminol, isoluminol, theromatic acridinium ester, imidazole, acridinium salt and oxalate ester.

[0187] Likewise, a bioluminescent compound may be used to label the antibody of the present invention. Bioluminescence is a type of chemiluminescence found in biological systems in, which a catalytic protein increases the efficiency of the chemiluminescent reaction. The presence of a bioluminescent protein is determined by detecting the presence of luminescence. Important bioluminescent compounds for purposes of labeling are luciferin, luciferase and aequorin.

[0188] Imaging Prostate Disease Conditions

[0189] In some cases, differentially expressed gene products identified herein may be up-regulated under prostate disease conditions and expressed on the surface of the affected tissue including such gene products comprising those known receptor proteins, structural proteins, peptidases and proteinases, membrane proteins, growth factors and cytokines as identified in Tables 1-6, and the as yet uncharacterized cell surface molecules as found in the unknown categories of Tables 1-6. Such target gene products allow for the non-invasive imaging of damaged or diseased prostate tissue for the purposed of diagnosis and directing of treatment of prostate disease.

[0190] Monoclonal and polyclonal antibodies which specifically bind to such surface proteins can be used for the diagnosis of prostate disease by in vivo tissue imaging techniques. An antibody specific for a target gene product, or preferably an antigen binding fragment thereof, is conjugated to a label (e.g., a gamma emitting radioisotope) which generates a detectable signal and administered to a subject (human or animal) suspected of having prostate disease. After sufficient time to allow the detectably-labeled antibody to localize at the diseased or damaged tissue site (or sites), the signal generated by the label is detected by a photoscanning device. The detected signal is then converted to an image of the tissue. This image makes it possible to localize the tissue in vivo. This data can then be used to develop an appropriate therapeutic strategy.

[0191] Antibody fragments, rather than whole antibody molecules, are generally preferred for use in tissue imaging. Antibody fragments accumulate at the tissue(s) more rapidly because they are distributed more readily than are entire antibody molecules. Thus, an image can be obtained in less time than is possible using whole antibody. These fragments are also cleared more rapidly from tissues, resulting in a lower background signal. See, e., Haber et al., U.S. Pat. No. 4,036,945; Goldenberg et al., U.S. Pat. No. 4,331,647. The divalent antigen binding fragment (Fab′)₂ and the monovalent Fab are especially preferred. Such fragments can be prepared by digestion of the whole immunoglobulin molecule with the enzymes pepsin or papain according to any of several well known protocols. The types of labels that are suitable for conjugation to a monoclonal antibody for diseased or damaged tissue localization include, but are not limited to radiolabels (i.e. radioisotopes), fluorescent labels and biotin labels.

[0192] Among the radioisotopes that can be used to label antibodies or antibody fragments, gamma-emitters, positron-emitters, X-ray-emitters and fluorescence-emitters are suitable for localization. Suitable radioisotopes for labeling antibodies include Iodine-131, Iodine-123, Iodine-125, Iodine-126, Iodine-133, Bromine-77, Indium-111, Indium-113m, Gallium-67, Gallium-68, Ruthenium-95, Ruthenium-97, Ruthenium-103, Ruthenium-105, Mercury-107, Mercury-203, Rhenium-99m, Rhenium-105, Rhenium- 101, Tellurium-121m, Tellurium-122m, Tellurium-125m, Thulium-165, Thulium-167, Thulium-168, Technetium-99m and Fluorine-18. The halogens can be used more or less interchangeably as labels since halogen-labeled antibodies and/or normal immunoglobulins would have substantially the same kinetics and distribution and similar metabolism.

[0193] The gamma-emitters Indium-111 and Technetium-99m are preferred because these radiometals are detectable with a gamma camera and have favorable half lives for imaging in vivo. Antibody can be labelled with Indium-111 or Technetium-99m via a conjugated metal chelator, such as DTPA (diethlenetriaminepentaacetic acid). See Krejcarek et al., 1977, Biochem. Biophys. Res. Comm. 77:581; Khaw et al., 1980, Science 209:295; Gansow et al., U.S. Pat. No. 4,472,509; Hnatowich, U.S. Pat. No. 4,479,930, the teachings of which are incorporated herein by reference.

[0194] Fluorescent compounds that are suitable for conjugation to a monoclonal antibody include fluorescein sodium, fluorescein isothiocyanate, and Texas Red sulfonyl chloride. See, DeBelder & Wik, 1975, Carbohydrate Research 44:254-257. Those skilled in the art will know, or will be able to ascertain with no more than routine experimentation, other fluorescent compounds that are suitable for labeling monoclonal antibodies.

[0195] Gene Therapy

[0196] Gene therapy was originally conceived of as a specific gene replacement therapy for correction of heritable defects to deliver functionally active therapeutic genes into targeted cells. Initial efforts toward somatic gene therapy relied on indirect means of introducing genes into tissues, called ex vivo gene therapy, e.g., target cells are removed from the body, transfected or infected with vectors carrying recombinant genes and re-implanted into the body (“autologous cell transfer”). A variety of transfection techniques are currently available and used to transfer DNA in vitro into cells; including calcium phosphate-DNA precipitation, DEAE-Dextran transfection, electroporation, liposome mediated DNA transfer or transduction with recombinant viral vectors. Such ex vivo treatment protocols have been proposed to transfer DNA into a variety of different cell types including epithelial cells (U.S. Pat. No. 4,868,116; Morgan and Mulligan WO87/00201; Morgan et al., 1987, Science 237:1476-1479; Morgan and Mulligan, U.S. Pat. No. 4,980,286), endothelial cells (WO89/05345), hepatocytes (WO89/07136; Wolff et al., 1987, Proc. Natl. Acad. Sci. USA 84:3344-3348; Ledley et al, 1987 Proc. Natl. Acad. Sci. 84:5335-5339; Wilson and Mulligan, WO89/07136; Wilson et al., 1990, Proc. Natl. Acad. Sci. 87:8437-8441), fibroblasts (Palmer et al., 1987, Proc. Natl. Acad. Sci. USA 84:1055-1059; Anson et al., 1987, Mol. Biol. Med. 4:11-20; Rosenberg et al., 1988, Science 242:1575-1578; Naughton & Naughton, U.S. Pat. No. 4,963,489), lymphocytes (Anderson et al, U.S. Pat. No. 5,399,346; Blaese, R M. et al., 1995, Science 270:475-480) and hematopoietic stem cells (Lim, B. et al 1989, Proc. Natl. Acad. Sci. USA 86:8892-8896; Anderson et al., U.S. Pat. No. 5,399,346).

[0197] Direct in vivo gene transfer recently has been attempted with formulations of DNA trapped in liposomes (Ledley et al., 1987, J. Pediatrics 110:1), in proteoliposomes that contain viral envelope receptor proteins (Nicolau et al., 1983, Proc. Natl. Acad. Sci. U.S.A. 80:1068) and DNA coupled to a polylysine-glycoprotein carrier complex. In addition, “gene guns” have been used for gene delivery into cells (Australian Patent No. 9068389). It even has been speculated that naked DNA, or DNA associated with liposomes, can be formulated in liquid carrier solutions for injection into interstitial spaces for transfer of DNA into cells (Felgner, WO90/11092).

[0198] Perhaps, one of the greatest problems associated with currently devised gene therapies, whether ex vivo or in vivo, is the inability to transfer DNA efficiently into a targeted cell population and to achieve high level expression of the gene product in vivo. Viral vectors are regarded as the most efficient system, and recombinant replication-defective viral vectors have been used to transduce (i.e., infect) cells both ex vivo and in vivo. Such vectors have included retroviral, adenoviral, adeno-associated viral and herpes viral vectors. While highly efficient at gene transfer, the major disadvantages associated with the use of viral vectors include the inability of many viral vectors to infect non-dividing cells, problems associated with insertional mutagenesis, inflammatory reactions to the virus and potential helper virus production and/or production and transmission of harmful virus to other human patients. In addition to the low efficiency of most cell types to take up and express foreign DNA, many targeted cell populations are found in such low numbers in the body that the efficiency of presentation of DNA to the specific targeted cell types is diminished even further.

[0199] Retroviruses represent one class of viruses that have been studied extensively for use in gene therapy (Miller, A. D., 1990, Human Gene Ther. 1:5-14). Unfortunately, there are a number of disadvantages associated with retroviral use, including the random integration of retroviruses into the host genome, which often leads to insertional mutagenesis or the inadvertent activation of proto-oncogene expression due to the promoter activity associated with retroviral LTRs (long terminal repeats). Adeno-associated viruses (“AAV”) also have been studied as an alternative system for delivery of stable genetic information into a cell. These viruses have the desirable feature of potentially integrating in specific regions of the host genome. However, the usefulness of both retroviral and AAV vectors is limited by their inability to accept heterologous DNA fragments greater than 3-5 Kb, their inability to produce larger quantities of viral stocks and, in the case of retroviruses, their instability and inability to infect non-dividing cells.

[0200] Some viral constructs, including those using retroviruses, are capable of stabile transfection of host cells, leading to long-term transgene expression. Adenoviruses, to the contrary, insert their DNA episomally, leading to transient gene expression for 2-4 weeks. For some disease processes, such as cystic fibrosis, permanent transgene expression clearly would be required (Cook S D, et al., 1996, Clinical Orthopedics and Related Research, 324:29-38). Thus, retroviral or adeno-associated viral vectors, which are capable of integrating into the hosts's genome, would be desirable for the treatment of these disease processes. For other diseases, wherein transgenes encode, for example, growth factors, transient expression may be advantageous, since prolonged gene expression could lead to serious side-effects. In these cases, a non-integrating viral vector, such as adenovirus, would be preferred.

[0201] Adenovirus Based Vectors

[0202] Adenovirus is a large, non-enveloped virus consisting of a dense protein capsid and a large linear (36 kb) double stranded DNA genome. Adenovirus infects a variety of both dividing and non-dividing cells, gaining entry by receptor-mediated uptake into endosomes, followed by internalization. After uncoating, the adenovirus genome expresses a large number of different gene products that are involved in viral replication, modification of host cell metabolism and packaging of progeny viral particles. Three adenovirus gene products are essential for replication of viral genomes: (1) the terminal binding protein which primes DNA replication, (2) the viral DNA polymerase and (3) the DNA binding protein (reviewed in Tamanoi and Stillman, 1983, Immunol. 109:75-87). In addition, processing of the terminal binding protein by the adenovirus 23 kDa L3 protease is required to permit subsequent rounds of reinfection (Stillman et al., 1981, Cell, 23:497-508) as well as to process adenovirus structural proteins, permitting completion of self-assembly of capsids (Bhatti and Weber, 1979, Virology, 96:478-485).

[0203] Packaging of nascent adenovirus particles takes place in the nucleus, requiring both cis-acting DNA elements and trans-acting viral factors, the latter generally construed to be a number of viral structural polypeptides. Packaging of adenoviral DNA sequences into adenovirus capsids requires the viral genomes to possess functional adenovirus encapsidation signals, which are located in the left and right termini of the linear viral genome (Hearing et al., 1987, J. Virol.61:2555-2558). Additionally, the packaging sequence must reside near the ends of the viral genome to function (Hearing et al., 1987, J. Virol. 61:2555-2558; Grable and Hearing, 1992, J. Virol., 66:723-731). The E1A enhancer, the viral replication origin and the encapsidation signal compose the duplicated inverted terminal repeat (ITR) sequences located at the two ends of adenovirus genomic DNA. The replication origin is defined loosely by a series of conserved nucleotide sequences in the ITR which must be positioned close to the end of the genome to act as a replication-priming element (reviewed in Challberg and Kelly, 1989, Biochem, 58:671-717; Tamanoi and Stillman, 1983, Immunol. 109:75-87). As shown by several groups, the ITRs are sufficient to confer replication to a heterologous DNA in the presence of complementing adenovirus functions. Adenovirus “mini-chromosomes” consisting of the terminal ITRs flanking short linear DNA fragments (in some cases non-viral DNAs) were found to replicate in vivo at low levels in the presence of infecting wild-type adenovirus, or in vitro at low levels in extracts prepared from infected cells (e.g., Hay et al., 1984, J. Mol. Biol. 175:493-510; Tamanoi and Stillman, 1983, Immunol. 109:75-87). Evidence for trans-packaging of mini-chromosomes was not reported in these or any later studies concerned with mechanisms of adenovirus DNA replication, and it is unlikely that packaging occurred for several reasons. First, the replicated molecules were quite small and they were not expressed at levels high enough to compete for packaging. Second, no selection for trans-packaging was employed, making it inconceivable that the heterologously replicated molecules could compete for packaging against wild-type adenovirus genomes.

[0204] The expression of foreign genes in “replication-defective” adenoviruses (deleted of region E1) has been exploited for a number of years in many labs, and a variety of published reports describe several different approaches often used in constructing these vectors (Vernon et al., 1991, J. Gen. Virol., 72:1243-1251; Wilkinson and Akrigg, 1992, Nuc. Acids Res., 20:2233-2239; Eloit et al., 1990, J. Gen. Virol., 71:2425-2431; Johnson, 1991; Prevec et al., 1990, J. Infect. Dis., 161:27-30; Haj-Ahmad and Graham, 1986, J. Virol., 57:267-274; Lucito and Schneider, 1992, J. Virol., 66:983-991; reviewed in Graham and Prevec, 1992, Butterworth-Heinemann, 363-393). In general, replication-defective viruses are produced by replacing part, or all, of essential region E1 with a heterologous gene of interest, either by direct ligation to vial genomes in vitro, or by homologous recombination within cells in vivo (procedures reviewed in Berkner, 1992, Curr. Topics Micro. Immunol., 158:39-66). These procedures all produce adenovirus vectors that replicate in complementing cell lines such as 293 cells which provide the E1 gene products in trans. Replication competent adenovirus vectors also have been described that have the heterologous gene of interest inserted in place of non-essential region E3 (e.g., Haj-Ahmad and Graham, 1986, J. Virol. 57:267-274), or between the right ITR and region E4 (Saito et al., 1985, J. Virol., 54:711-719). In both, replication defective viruses and replication competent viruses, the heterologous gene of interest is incorporated into viral particles by packaging of the recombinant adenovirus genome.

[0205] Some viral constructs, including those using retroviruses, are capable of stable transfection of host cells, leading to long-term transgene expression. Adenoviruses, to the contrary, insert their DNA episomally, leading to transient gene expression for 2-4 weeks. For some disease processes, such as cystic fibrosis and osteoporosis, permanent transgene expression clearly would be required (Cook S D, et al., 1996, Clinical Orthopedics and Related Research, 324:29-38). Thus, retroviral or adeno-associated viral vectors, which are capable of integrating into the hosts's genome, would be desirable for the treatment of these disease processes. For other diseases, wherein transgenes encode, for example, growth factors, transient expression may be advantageous, since prolonged gene expression could lead to serious side-effects. In these cases, a non-integrating viral vector, such as adenovirus, would be preferred.

[0206] One may obtain the DNA segment encoding the protein of interest using a variety of molecular biological techniques, generally known to those skilled in the art. For example, cDNA or genomic libraries may be screened using primers or probes with sequences based on the known nucleotide sequences. Polymerase chain reaction (PCR) also may be used to generate the DNA fragment encoding the protein of interest. Alternatively, the DNA fragment may be obtained from a commercial source.

[0207] The DNA encoding the translational or transcriptional products of interest may be engineered recombinantly into a variety of vector systems that provide for replication of the DNA in large scale for the preparation of the viral vectors of the invention. These vectors can be designed to contain the necessary elements for directing the transcription and/or translation of the DNA sequence taken up by the bone cells at the repair site in vivo.

[0208] Methods which are well known to those skilled in the art can be used to construct expression vectors containing the protein coding sequence operatively associated with appropriate transcriptional/translational control signals. These methods include in vitro recombinant DNA techniques, and synthetic techniques. See, for example, the techniques described in Sambrook, et al., 1992, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, N.Y. and Ausubel et al., 1989, Current Protocols in Molecular Biology, Greene Publishing Associates & Wiley Interscience, N.Y.

[0209] The genes encoding the proteins of interest may be associated operatively with a variety of different promoter/enhancer elements. The expression elements of these vectors may vary in their strength and specificities. Depending on the host/vector system utilized, any one of a number of suitable transcription and translation elements may be used. The promoter may be in the form of the promoter which is associated naturally with the gene of interest. Alternatively, the DNA may be positioned under the control of a recombinant or heterologous promoter, i.e., a promoter that is not associated normally with that gene. For example, tissue specific promoter/enhancer elements may be used to regulate the expression of the transferred DNA in specific cell types. Examples of transcriptional control regions that exhibit tissue specificity which have been described and could be used, include, but are not limited to: elastase I gene control region which is active in pancreatic acinar cells (Swift et al., 1984, Cell 38:639-646; Ornitz et al., 1986, Cold Spring Harbor Symp. Quant. Biol. 50:399-409; MacDonald, 1987, Hepatology 7:42S-51S); insulin gene control region which is active in pancreatic beta cells (Hanahan, 1985, Nature 315:115-122); immunoglobulin gene control region which is active in lymphoid cells (Grosschedl et al., 1984, Cell 38:647-658; Adams et al., 1985, Nature 318:533-538; Alexander et al., 1987, Mol. Cell. Biol. 7:1436-1444); albumin gene control region which is active in liver (Pinkert et al., 1987, Genes and Devel. 1:268-276); alpha-fetoprotein gene control region which is active in liver (Krumlauf et al., 1985, Mol. Cell. Biol. 5:1639-1648; Hammer et al., 1987, Science 235:53-58); alpha-1-antitrypsin gene control region which is active in liver (Kelsey et al., 1987, Genes and Devel. 1:161-171); beta-globin gene control region which is active in myeloid cells (Magram et al., 1985, Nature 315:338-340; Kollias et al., 1986, Cell 46:89-94); myelin basic protein gene control region which is active in oligodendrocyte cells in the brain (Readhead et al., 1987, Cell 48:703-712); myosin light chain-2 gene control region which is active in skeletal muscle (Shani, 1985, Nature 314:283-286) and gonadotropic releasing hormone gene control region which is active in the hypothalamus (Mason et al., 1986, Science 234:1372-1378). Promoters isolated from the genome of viruses that grow in mammalian cells, other than the CMV promoter, (e.g., RSV, vaccinia virus 7.5K, SV40, HSV, adenoviruses MLP, and MMTV LTR promoters) may be used, as well as promoters produced by recombinant DNA or synthetic techniques.

[0210] The use of tissue specific promoters to drive therapeutic gene expression would decrease further a toxic effect of the therapeutic gene on neighboring normal cells when virus-mediated gene delivery results in the infection of the normal cells. This would be important especially in diseases where systemic administration could be utilized to deliver a therapeutic vector throughout the body, while maintaining transgene expression to a limited and specific number of cell types. Moreover, since many growth factors, such as TGF-β, have pleiotropic effects, numerous, harmful side effects likely would be exhibited if the growth factor genes are expressed in all cells.

[0211] In some instances, the promoter elements may be constitutive or inducible promoters and can be used under the appropriate conditions to direct high level or regulated expression of the gene of interest. Expression of genes under the control of constitutive promoters does not require the presence of a specific substrate to induce gene expression and will occur under all conditions of cell growth. In contrast, expression of genes controlled by inducible promoters is responsive to the presence or absence of an inducing agent. For example, if a cell is stably transfected with a therapeutic, inducible transgene, its expression could be controlled over the life-time of the individual.

[0212] Specific initiation signals also are required for sufficient translation of inserted protein coding sequences. These signals include the ATG initiation codon and adjacent sequences. In cases where the entire coding sequence, including the initiation codon and adjacent sequences, are inserted into the appropriate expression vectors, no additional translational control signals may be needed. However, in cases where only a portion of the coding sequence is inserted, exogenous translational control signals, including the ATG initiation codon, must be provided. Furthermore, the initiation codon must be in phase with the reading flame of the protein coding sequences to ensure translation of the entire insert. These exogenous translational control signals and initiation codons can be of a variety of origins, both natural and synthetic. The efficiency and control of expression may be enhanced by the inclusion of transcription attenuation sequences, enhancer elements, etc.

[0213] In addition to DNA sequences encoding therapeutic proteins of interest, the scope of the present invention includes the use of ribozymes or antisense DNA molecules that may be transferred into mammalian cells. Such ribozymes and antisense molecules may be used to inhibit the translation of RNA encoding proteins of genes that promote the prostate disease process.

[0214] The expression of antisense RNA molecules will act directly to block the translation of mRNA by binding to targeted mRNA and preventing protein translation. The expression of ribozymes, which are enzymatic RNA molecules capable of catalyzing the specific cleavage of RNA, also may be used to block protein translation. The mechanism of ribozyme action involves sequence specific hybridization of the ribozyme molecule to complementary target RNA, followed by an endonucleolytic cleavage. Within the scope of the invention are engineered hammerhead motif ribozyme molecules that specifically and efficiently catalyze endonucleolytic cleavage of RNA sequences. RNA molecules may be generated by transcription of DNA sequences encoding the RNA molecule.

[0215] It also is within the scope of the invention that multiple genes, combined on a single genetic construct under control of one or more promoters, or prepared as separate constructs of the same or different types, may be used. Thus, an almost endless combination of different genes and genetic constructs may be employed. Certain gene combinations may be designed to, or their use may otherwise result in, achieving synergistic effects in amelioration of prostate disease, and any and all such combinations are intended to fall within the scope of the present invention. Indeed, many synergistic effects have been described in the scientific literature, so that one of ordinary skill in the art readily would be able to identify likely synergistic gene combinations, or even gene-protein combinations. It will also be appreciated to those skilled in the art that the invention can be performed within a wide range of equivalent parameters of composition, concentration, modes of administration, and conditions without departing from the spirit or scope of the invention or any embodiment thereof.

[0216] Having now fully described the invention, the same will be more readily understood by reference to specific examples which are provided by way of illustration, and are not intended to be limiting of the invention, unless herein specified.

EXAMPLE 1

[0217] Current staging and prognostic modalities for human prostate cancer are inadequate. Furthermore, our comprehension of the genetics of prostate carcinogenesis is lacking, although several genetic and epigenetic factors have been identified that correlate with the development of a more aggressive neoplastic phenotype. In the human, mesenchymal-epithelial interaction maintains the functional integrity of the adult prostate gland. Prior investigations in this laboratory have demonstrated that fetal mesenchyme has the capacity to initiate glandular overgrowth of the adult rodent prostate (McKinnell et al., New York:Plenum Press, 1989; Sikes et al., Biology of Reproduction. 43:353-62, 1990), reduce anaplasia in the Dunning prostatic adenocarcinoma model (Chung et al., Prostate. 17:165-74, 1990; Hayashi et al., Cancer Research. 50:4747-54, 1990), and induce the differentiation of androgen receptor-deficient urogenital sinus epithelium (UGE) into functional prostate tissue (Sikes et al., Biology of Reproduction. 43:353-62,1990; Chung et al.,Molecular Biology Reports. 23:13-19,1996; Bissell et al., The Journal of Theoretical Biology. 99:31-68, 1982).

[0218] Prostatic carcinogenesis may be explained by aberrant instructive influences derived from its underlying stroma, as the microenvironment surrounding the cancer epithelium has been demonstrated to determine tumor growth and malignant potential (Drews et al., Cell. 10:401-404,1977; Franks et al., The Journal of Pathology. 100:113-120,1970). Consequently, it is believed that abnormal prostate growth and carcinogenesis may result from abnormalities in the constituents of the stromal-epithelial milieu. The inductive role of stroma has been demonstrated in a wide variety of glandular tissues during embryonic development, including the prostate (Bissell et al., The Journal of Theoretical Biology. 99:31-68, 1982; McNeal, Investigative Urology. 15:340-5,1978; Cunha et al., Journal of Steroid Biochemistry. 14:1317-24, 1981; Cunha et al., Biology of Reproduction. 22:19-42, 1980; Chung et al., Prostate. 4:503-11, 1983; Cunhaetal., Endocrine Reviews.8:338-62,1987). Prostatic proliferation in the adult may result from a reawakening of dormant embryonic growth elements present in the prostatic stroma (Pierce, N.J.: Prentiss-Hall, Inc., 1978). It has been demonstrated that fetal urogenital sinus mesenchyme (UGM), a fetal form of prostatic stroma, is inductive and can redirect prostatic epithelial growth and differentiation (Sikes et al., Biology of Reproduction. 43:353-62, 1990; Chung et al., Biology of Reproduction. 31:155-163, 1984; Gleave et al., Cancer Research. 51:3753-61, 1991). Marked growth and expression of tissue-specific secretory proteins can be induced when fetal UGM is recombined with either fetal or adult prostate epithelium (Chung, Cancer Surveys. 23:33-42,1995; Evans, The British Journal of Cancer. 68:1051 -1060, 1993) or when it is implanted directly into the adult prostate gland (Han et al., Carcinogenesis. 16:951-954, 1995). Implanted fetal mesenchyme can induce differentiation and growth of adult rat urogenital cells (Chung et al., Prostate. 17:165-74, 1990; Hayashi et al., Cancer Research. 50:4747-54, 1990). Recombinants of androgen receptor deficient fetal mesenchyme with either fetal or adult epithelium failed to produce appropriate cytodifferentiation when recombined with fetal UGM lacking the androgen receptor (derived from testicular feminization, Tfm/y, fetuses) (Sikes et al., Biology of Reproduction. 43:353-62, 1990). This further supports the contention that paracrine mediators between stroma and epithelium are prerequisite for prostate growth and morphogenesis.

[0219] Inductive influences from stroma to prostatic epithelial differentiation can be classified as either directive or permissive, depending upon the sources of embryonic epithelium and the age of both the inductive and responsive fetal tissue (Cunha et al., Recent Progress in Hormone Research. 39:559-98, 1983). Thereafter, the ultimate growth potential of the embryonic and adult prostatic epithelium in tissue recombinants or in situ will be dictated by the presence and origin of inductive stroma. By varying the amount of embryonic stroma used in the construction of tissue recombinants (Evans, The British Journal of Cancer. 68:1051-1060, 1993) or by inserting fetal UGM directly into the adult prostate (Han et al., Carcinogenesis. 16:951-954, 1995), it has been shown that the growth potential of prostatic epithelium is dictated entirely by the amount of UGM present in either tissue recombinants or in the induced chimeric adult gland. Hence, mesenchymal agents can induce normal and neoplastic prostate growth and differentiation. This implies that the adult epithelium is capable of responding to a fetal inducer that is no longer present in normal prostate tissue. Furthermore, prostate carcinogenesis mimics a reversion to a more developmentally primitive state. Therefore, the differential expression of prostate-fetal genes may direct neoplastic transformation or at least identify when a clonal population has undergone such transformation.

[0220] The temporal involvement of steroid hormones and growth factors is paramount to prostate development. Prostate growth and differentiation is tightly regulated by androgens and is influenced by a number of soluble peptide growth factors and their receptors (Sokoloffet al., Cancer. 77:1862-1872, 1996). A close reciprocal association between stromal and epithehal tissues also has a fundamental role in normal, benign, and malignant prostate development. Mesenchymal and epithelial differentiation depends upon the stimulatory effects of dihydrotestosterone, inductive growth factors and peptides, and embryonic factors (Sokoloff et al., Cancer. 77:1862-1872, 1996). The combination of epidermal growth factor, transforming growth factor-β, insulin growth factor, and gonadotropin can induce differentiation of reproductive cells. Other studies have demonstrated that many of the properties associated with tumor progression and metastasis in hormone-refractory prostate cancer cell lines can be altered after treatment with cytokines (Ritchie et al., Endocrinology. 138:1145-1150, 1997; Ausubel et al., Preparing DNA from small-scale liquid lysates. In: K. Janssen (ed.) Current protocols in molecular biology., Vol. 1, pp. Section 1.13.7. New York: John Wiley and Sons, Inc., 1994). These studies found that suppression of prostate cancer cell growth correlated with the down regulation of oncogene, suppressor gene, growth factor, and adhesion molecule gene expression. Currently, there are no fetal-prostate markers described in prostate cancer for use as either diagnostic or prognostic markers. Therefore, this study describes the isolation of novel fetal prostate-derived genes for the purpose of developing prostatic markers. Further, for the first time fetal prostate genes are shown to be (re)expressed in prostate cancer cell lines.

[0221] The hypothesis to be tested in the present study is that fetal UGS-derived gene (re)expression or loss is important in the development and progression of prostate cancer. Furthermore, these genes encode oncofetal proteins that can serve as diagnostic, prognostic and therapeutic targets for use in the management of human prostate cancer. This study presents the cloning, characterization, and examination of the expression and possible role of a single differentially-expressed fetal UGS-derived gene, UG311, in cell lines and human prostate cancer specimens.

[0222] Aim

[0223] To clone and characterize the full-length cDNA corresponding to the differentially expressed urogenital sinus-derived expressed sequence tags, UG311, from LNCaP or C4-2 lambda gt11 cDNA libraries or by 5′- and 3′-RACE.a) Urogenital sinus (UGS)-derived expressed sequence tags will be used as probes to identify homologous phage inserts in LNCaP or C4-2 cDNA libraries. Overlapping contigs will be assembled as required. b) Alternatively, UGS-derived EST homologs will be cloned using 5′-3′- rapid amplification of cDNA ends (RACE) using LNCaP and C4-2 as mRNA as starting materials. Sequences obtained will be compared to those from lambda phage inserts and a closely related GenBank sequence nmt55.

[0224] Experimental Approach

[0225] The original UG311 insert was sequenced bidirectionally and found to contain an insert of 682 bp. The GenBank analysis of this insert revealed -98% homology to a drosophila protein, nonA^(diss) and the putative mammalian homologue NonO (Mahana et al., Journal of Immunological Methods. 161:187-192,1993). NonA/NonO has been described as a non-POU domain octamer-binding protein. Octamer binding proteins (OBP) are transcription factors that regulate the expression of a wide range of genes. This occurs from both the direct interaction of the OBP with DNA as well as the OBP's interaction with other transcription factors to determine the final modulation of a particular gene's transcriptional rate (Harlow et al., Antibodies:A laboratory Manual., pp. 726. New York:Cold Spring Harbor Laboratory, 1988; Sikes et al., Cancer Research. 52:3174-81, 1992).

[0226] Classical OBPs, those that contain a POU-domain, have family members that are ubiquitously expressed as well as those that have tissue-restricted expression patterns (Zhau et al., The Prostate. 28:73-83, 1996; Marengo et al., Molecular Carcinogenesis. In Press:, 1997). Those with tissue specific expression have been shown to be important in the development and maintenance of that cell phenotype (Zhau et al., The Prostate. 28:73-83, 1996; Marengo et al., Molecular Carcinogenesis. In Press:, 1997). The ubiquitous NonO/NonA mRNA was shown to have an open reading frame of 1418 bp encoded by a 2.4 kb cDNA (Mahana et al., Journal of Immunological Methods. 161:187-192, 1993). RNA blot analysis indicated ubiquitous expression of a 1.6 kb RNA with a band present also in mouse prostate tissue. The largest and tissue-specific mRNA described for NonO/NonA was 3.8 kb found exclusively in the retina. RNA blot analysis using UG311 as a probe on prostate cancer cell line RNA (FIG. 3) gave an initial mRNA signal corresponding to 3.2 kb. This data implied that either UG311 is a member of a family related to the NonO/NonA gene or represents a novel splice variant.

[0227] To investigate these possibilities cDNA primers were synthesized to the UG311 sequence in order to perform 5′- and 3′-rapid amplification of cDNA ends (RACE). RACE reactions were performed according to the manufacturer's recommendations except that the internal primer set was subjected to a ramp-up annealing scheme instead of a ramp-down format. The resultant fragments were cloned into pCR2.1 TOPO-TA and were sequenced to confirm overlap between UG311 and the 5′-RACE clones. Two of six RACE fragments had identity in the 150 bp overlap. One other clone had homology only to the primer and the sequence diverged after that point suggesting either spurious priming or the existence of other NonO/NonA family members. These cloned 5′-RACE products extended the UG311 sequence to nearly 1500 bp. Resubmission of this contig. for FASTA to GenBank (data not shown) resulted in the discovery of two nearly identical sequences, nmt55 and p54nrb. The identity of these sequences to UG311-1500 bp was nearly 99% while that of NonO/NonA dropped to 92%. The nmt55 protein was found by screening antibodies generated against the polybasic repeat region of the human estrogen receptor (Sikes et al., Molecular Biology and Biochemistry, pp. 156. Houston:University of Texas Graduate School of Biomedical Sciences, 1993). Western blotting showed no reactivity of these antisera to the estrogen receptor. Instead, there was strong reactivity to an unrelated 55 kDa protein, nmt55/p54nrb is a protein identical to nmt55, found by using antibodies to a yeast mRNA splicing factor to screen a HeLa cDNA expression library (Rajagopal et al., International Journal of Cancer. 62:661-667, 1995). The resultant protein and cDNA bear no resemblance to the yeast splicing factor; however, there was extensive homology to human splicing factor PSF and to drosophila NonA. In HeLa the predominant transcript size was 2.6 kb with a very minor band at 1.9. The open reading frame is virtually identical to nmt55 (Rajagopal et al., International Journal of Cancer. 62:661-667, 1995). This protein was found to be localized to the nucleus and to bind to both single- and double-stranded nucleic acids (Mahana et al., Journal of Immunological Methods. 161:187-192, 1993). Furthermore, nmt55/p54nrb has been demonstrated to facilitate the association of other DNA-binding factors, e.g. topoisomerase I and Ku80, to DNA as well as have a direct role in the transcriptional machinery (Hsieh et al., Cancer Research. 55:190-7, 1995; Southern et al., Journal of Molecular Biology. 98:503-, 1975;Laemmli et al., Nature (London). 227:680-685, 1970). For these reasons nmt55 is thought to be important in either RNA-splicing or DNA repair processes. Additionally, western blotting from normal and cancerous breast samples revealed the loss of nmt55 with the progression of the breast cancer (Sikes et al., Molecular Biology and Biochemistry, pp. 156. Houston:University of Texas Graduate School of Biomedical Sciences, 1993). Interestingly, the open reading frame of nmt55 and p54nrb is found in the first 1600 bases. Thus, if the 5′-RACE of UG311 actually extended to the 5′-end of the mRNA then these genes could be homologous, except for the fact that the longest cDNA for either nmt55 or pnrb54 is only 2.7-2.9 kb or 300-500 bp shorter than the mRNA found in the prostate cancer cell lines.

[0228] Therefore, it is of interest to determine the basis for the difference in mRNA lengths of these described related species. Since nmt55 assists other DNA repair enzymes in binding to DNA or may be involved RNA splicing and transcription, it is likely that this protein or other family members represent critical molecules in either cell survival or cell stability. Therefore, cloning and characterization UG311 to determine if it is related to nmt55 or simply another splice variant of a larger mRNA to give the same open reading frame represents a novel and potentially significant step towards understanding a mechanism for prostate cancer progression. The fact that this is lost with breast cancer progression and down regulated in the LNCaP-C4-2 prostate cancer model system implies a functional significance and potential utility for nmt55/UG311 as a prostate cancer marker. For these reasons this study focuses on the cloning and characterization of UG311 to determine the relationship to nmt55 and its role in the biological behavior of prostate cancer. This is the first description, of either a fetal prostate-derived gene or a putative DNA association factor in prostate cancer cell lines with a correlation to progression.

[0229] Aim

[0230] The cloning and characterization of the full-length cDNA corresponding to the differentially expressed urogenital sinus-derived expressed sequence tag, UG311, from LNCaP or C4-2 lambda gt11 cDNA libraries or by 5′- and 3′-RACE.

[0231] Rationale

[0232] As described above, it is important to know whether UG311 represents a novel gene closely related to nmt55/nrb54 or merely a splice variant, or processing variant leading to a longer mRNA in prostate. The presence of additional coding sequence would provide clues to tissue-specific RNA splicing or transcription control while additional 3′noncoding sequence may provide information on mRNA stability or potentially tissue specific interactions with other single-stranded nucleic acid binding proteins that associate with these sequences. Therefore, it is necessary to clone the UG311 homolog from prostate cell lines or tissues.

[0233] Experimental Approach

[0234] Cloning of UG311 cDNA from Lambda gt11 Expression Library

[0235] All cell lines and libraries are to be made available from Dr. Leland Chung, Ph.D. LNCaP and C4-2 cell line lambda gt11 phage libraries will be screened for homologous clones to UG311. These clones will be sequenced to determine homology and overlap. Overlapping clones will be reassembled by subcloning with available restriction enzyme sites. These libraries were constructed from poly A+ selected RNA using Invitrogen Custom Services (Invitrogen Corp., San Diego, Calif.) and these libraries have been used previously to clone cDNAs corresponding to differential display PCR fragments (Chen et al JBC 1998). Following long-term storage at −80° C., these libraries will be retitered before screening. Up to one million plaques will be screened for each novel UGS-derived EST. At least 3 plaques will be purified through three rounds of hybridization (Gleave et al., Cancer Research. 52:1598-605, 1992). Hybridization conditions between mouse and human cDNAs have been determined empirically and are performed overnight at 60° C. in 5× standard saline citrate, 10% high molecular weight dextran sulfate, 15% formamide. Preparation of phage DNA will be accomplished by eluting phage from the purified plaques essentially as described (Gleave et al., Cancer Research. 52:1598-605, 1992; Ma et al., Fundamental and Clinical Pharmacology. 10:97-115, 1996). Phage pellets are resuspended in 200 μl Tris-Cl pH 8.0. Polymerase chain reaction (PCR) will be performed on the purified phage to determine the insert size and provide additional template for sequencing after cloning in to TA cloning vectors (Invitrogen Corp., San Diego Calif.).

[0236] The use of RACE Reactions to Generate UG311 cDNA

[0237] 5-prime and 3′-prime race will be performed using the Clontech kit (Clontech, Palo Alto, Calif.). 1 mg of total RNA from the LNCaP cell line will be reverse transcribed. The RNA will be digested and a second strand made. The 5′ and 3′ adapters are then ligated to the double-stranded cDNAs in separate reactions. PCR is then performed with a 5′ adapter specific upstream primer and a gene specific downstream primer as per the manufacturer's recommendations. The PCR products will be evaluated electrophoretically, gel purified, TA cloned as described above and sequenced. Any additional sequence obtained will be subcloned onto the phage-derived cDNA with care taken to exclude RACE primer sequences. Alternatively, RACE reactions can be used to generate the entire homologous cDNA using only overlapping forward and reverse gene specific primers. In this case, the primers would be synthesized from UGS-derived EST's. These RACE products would then be assembled into a contig. and compared to the sequence obtained from the phage inserts. The RACE procedure has been used to acquire an additional 800 bp of the 5′ end of UG311 to yield 1500 bp of sequence to date. Therefore the technique will be repeated on the 3′ end and the overall product compared to the phage insets obtained above.

EXAMPLE 2

[0238] Aim

[0239] To screen human prostate cancer specimens by immunohistochemistry (IHC) and in situ hybridization (ISH) for the expression of UG311 (nmt54) to determine if a significant correlation of UG311 expression to stage and grade, prognosis or patient survival exists.

[0240] Rationale

[0241] Antibodies to nmt55/nrb54 can be generated using routine methods well known in the art. Since nmt55 has virtual identity over most of the putative open reading frame with UG311-1500, its staining pattern should reflect the pattern that would be observed for UG311. Also, for a marker to be useful it must be able to distinguish between either the presence or absence of disease or be able to determine prognosis. Markers with such properties allow for patients to be stratified for either more or less aggressive therapeutic options. Therefore, this study seeks to determine if such a correlation exists for nmt55/UG311 in human prostate cancer specimens.

[0242] Experimental Approach

[0243] A cohort of 72 prostate cancer specimens will be examined by ISH and IHC. IHC was performed on both fresh frozen and paraffin embedded specimens (Sikes and Chung, Cancer Res. 1992). IHC will be done by the indirect calorimetric detection using DAB as the chromagen donor to a horse-radish peroxidase conjugated secondary antibody. Additionally, Dr. Robert Moreland has supplied detailed protocols for the nmt55 antibodies that include IHC, western blotting and immunoprecipitation (Sikes et al., Molecular Biology and Biochemistry, pp. 156. Houston:University of Texas Graduate School of Biomedical Sciences, 1993). The degree of staining will be scored and the tabulated data will be analyzed for significance and correlation to survival and staging.

[0244] Since some tissues will not react to IHC and others not to ISH, both will be done to fully cover the expression of nmt55 in the cohort. Furthermore, ISH provides complementary data on the localization of the mRNA for comparison to the localization of the protein. Colocalization is anticipated. Briefly, the protocol for non-radioactive ISH on paraffin embedded tissue sections is as follows: In situ hybridization will be performed using 30 ng of probe for each slide including antisense probes, sense probes as negative controls, and β-actin probes as positive controls as previously described (Gotoh et al., The Journal of Urology. In Press:, 1997; Akiyama et al., Fibronectin and integrins in invasion and metastasis., Cancer metastasis and reviews. 14:173-189, 1995). The tissue distribution of UG311 and β-actin will be determined by immunohistochemical staining methods developed in our laboratory. The intensity and the distribution of mRNA staining will be scored as follows: ++, diffuse localization in >25% of cells; +, focal localization in <25% of cells; −, negative.

[0245] If significant differences in UG311 cDNA and the nmt55 open reading frame are found, then the UG311 cDNA will be cloned into bacterial expression vector for amplification and purification. Purified UGS derived gene fusion-proteins will be used as an immunogen for the generation of polyclonal antibodies. Antibodies will be tested for reactivity under reducing and nonreducing conditions as well as paraffin-embedded and frozen tissue sections.

[0246] The purpose of this study is to generate several high quality antibodies against the UG311 gene product to facilitate the study of its biology and biochemistry in prostate cancer. Antibodies are desired that will react positively to the UG311 gene product in immunohistochemistry (cell lines and paraffin sections) western blots (reduced and non-reducing conditions) and immunoprecipitation. Since peptide-derived antibodies frequently fail to work well for all biochemical applications, the use of peptides to generate antibodies will be an alternative secondary option. First, fusion proteins will be generated from the UG311 ORF for the production of antibodies.

[0247] Bacterial expression and purification of many proteins or protein fragments has allowed for the generation of antibodies to a wide variety of proteins including difficult, i.e., poorly immunogenic or highly conserved proteins (Ziober et al., Seminars in Cancer Biology. 7:119-128, 1996). This strategy will be employed to generate large amount of purified UG311 gene product. The UG311 ORF will be cloned into bacterial expression vector, pGEX-4T (Pharmacia Biotech, Piscataway N.J.) (FIG. 7). This plasmid generates a glutathione S-transferase (GST) fusion protein with the protein of interest when expressed in appropriate bacterial hosts. The GST portion allows for both the facilitated monitoring of fusion protein expression using a solution-based calorimetric assay in crude cell lysates as well as the ease of protein purification using a glutathione column. Polymerase chain reaction will be used to amplify the UG311 ORF incorporating appropriate in-frame restriction endonuclease sites for directional subcloning. This approach allows one to bypass any potential 5′-UTR that may be present and directly clone the UG311 coding sequences in-frame behind the GST fusion tag. UGS-derived gene products will be purified from bacterial hosts according to the manufacturer's recommendations. For pGEX4T expressed protein, purification will be accomplished by binding to a glutathione column followed by thrombin cleavage to remove the GST fusion protein. Thrombin will be removed by passing the eluate over a benzamidine sepharose column. Rapid preliminary detection of GST-fusion constructs can be ascertained by using a GST-detection kit (Pharmacia, Piscataway N.J.). Protein yield will be estimated by Bradford and purity followed by SDS-PAGE in 12.5 to 15% acrylamide gels in both systems.

[0248] EXAMPLE 3

[0249] Aim

[0250] To assess the possible direct and indirect biologic functions of the UG311/nmt55 in prostate cancer progression.

[0251] Rationale

[0252] Since nmt55 has been shown to be lost in breast cancer progression and is associated with estrogen receptor negativity, a major prognostic factor for breast cancer, then it follows that the expression of nmt55/UG311 should be manipulated in prostate cancer cell lines to directly test whether or not the loss/overexpression of nmt55UG311 protein can modulate the aggressiveness of prostate cancer. Levels of UG311 gene expression in the LNCaP model of human prostate cancer progression will be manipulated using an inducible mammalian expression system (TET-on) in conjunction with protein tagging by using a FLAG epitope. It will be determined if the overexpression of these UGS-derived genes may decrease prostate cancer growth, invasiveness and/or metastatic potential. Conversely, suppressing the levels of UG311 gene expression by antisense technology may confer increased tumorigenicity and metastatic potential.

[0253] Since, the LNCaP/C4-2 model closely mimics the natural progression of human prostate cancer from non-metastasizing, androgen-dependent cells (LNCaP) that are gradually transformed in vivo into aggressively-metastasizing, androgen-independent cells (C4-2) this model represents an ideal system to test UG311 function by reducing the protein levels in LNCaP and by re-expression of the protein in C4-2 cells. Never before have fetal urogenital sinus-derived genes been associated with the malignant potential of prostate cancer. Further characterization of this gene and others should clarifying the role of embryonic influences on prostate carcinogenesis, as well as identify and develop novel prognostic markers and potential targets for gene therapy and other therapeutic modalities for treating human prostate cancer.

[0254] Experimental Approach

[0255] Example 2 presented above already examines whether or not there is a similar loss of nmt55/UG311 expression between human breast and prostate cancer tissues; this study will manipulate the gene product levels in a human prostate cancer progression model by using sense and antisense gene expression techniques in an inducible vector system to directly test the effects of UG311 protein levels on prostate cancer cell behavior. In order to assess the possible direct and indirect biologic functions of these genes in prostate cancer progression, the levels of UG311 expression in the experimental LNCaP model of human prostate cancer progression will be manipulated. This study will determine if overexpression of these genes may arrest prostate cancer growth and decrease its invasiveness and metastatic potential. Conversely, antisense constructs will be used to lower the steady-state levels of UG311 in the hope that reduced expression will increase invasive and metastatic potential.

[0256] Previously, several cDNA constructs in both rat and human prostate cancer cell lines have been cloned, transfected and overexpressed (Sikes and Chung, Cancer Res. 1992) (Levine et al., EXS. 74:157-179, 1995; Nagle et al.,, Journal of Cellular Biochemistry, Supplement. 19:232-237, 1994; Umbas et al., Cancer Research. 52:5104-9,1992). Overexpression of sense cDNAs has been employed with some success to evaluate gene product function in prostate cell lines (Levine et al., EXS. 74:157-179, 1995; Umbas et al.,Cancer Research. 52:5104-9, 1992; Freeman et al., Cancer Research. 51:1910-6, 1991). Likewise, antisense strategies employing full-length cDNA constructs have proven effective for the EGF receptor in colon carcinoma and C-CAM in prostate epithelia (Bussemakers et al., Cancer Research. 52:2916-22, 1992; Chung et al., Journal of Cellular Biochemistry—Supplement. 16H:99-105, 1992). Tet-responsive clones for LNCaP and C4-2 have already been generated using the TET-on system from Clontech and have been shown to induce the level of luciferase reporter gene expression by more than 125 fold (FIG. 4). Sense and antisense constructs of UG311 fused to FLAG-tag element will be amplified by PCR and subcloned into the VP-16 responsive vector for Doxycycline (TET) induction. Protein levels can be followed by both anti-FLAG and nmt55 antibodies. Sense and antisense riboprobes will follow the levels of RNA produced by RNA blot. One correct sense and one correct antisense clone will be expanded, purified by CsCl banding and sequenced by dideoxy chain termination using the ALF/express system from Pharmacia and Cy5 amidite fluorescent primers to confirm sequence fidelity and orientation.

[0257] Western blots will be performed as described in Sikes and Chung (Nagle et al., Journal of Cellular Biochemistry, Supplement. 19:232-237,1994) in the presence of protease inhibitors to determine the levels of UG311 gene products being expressed in the transfected cell lines. Enhanced chemiluminescent (ECL) detection of the UG311 protein will be performed according to the manufacturer's recommendations (Amersham, Arlington Heights, Ill.).

[0258] One of the sense and antisense UG311 tranfected clones, selected as described above, will be assessed for changes in their tumorigenic behavior by determining both anchorage independent growth, their cell migration/invasive potential in Matrigel® and tumor development in vivo as determined by subcutaneous (s.c.) injections into athymic male mouse hosts. Anchorage independent growth of sense and antisense clones will be assessed as described previously (Wu et al., The International Journal of Cancer. Submitted October 1997, 1997) (Inventors: please confirm the citation for this reference). Either 1000 or 5000 cells/ 6-well chamber will be mixed with an equal volume (1 ml) of low melting point agarose in distilled H₂O. Cells will be monitored for 6-8 weeks at which time colonies ≧0.4 mm diameter will be counted using a dissection microscope. Modified Boyden chamber assays will be used to assess tumor cell migration and invasiveness. The results of invasion assays will be correlated to the steady-state levels of UG311 protein expressed in the clones.

[0259] For tumorigenicity in vivo (Thalmann et al., Cancer Research. 54:2577-2581,1994; Wu et al., The International Journal of Cancer. Submitted October 1997, 1997), transfected cells prepared as above will be resuspended in T-medium/5% FBS at the appropriate cell number and injected using a graduated insulin syringe. UG311-Flag-Tet-on sense and antisense transfected LNCaP and C4-2 cell clones will be injected into intact nude mice at 4×10⁶ cells per 100 μl s.c. Tumors will be allowed to develop for 6 weeks or until the tumor mass has reached 1.5 cc at which time the animals will be euthanized. Tissues will be harvested, fixed in neutral-buffered formalin for less than 16 hrs, and sent to the pathology department for paraffin embedding and sectioning. Slides will be routinely stained with hematoxylin and eosin and read by the pathologist to determine the presence of cancer cells. Sections will be stained additionally as in Sikes and Chung (1992)(Nagle et al.,, Journal of Cellular Biochemistry, Supplement. 19:232-237, 1994) or Gleave et al (1992) (Liotta et al., Annual Review of Biochemistry. 55:1037-1057, 1986) for Ki67, PSA and tunel to monitor the extent of prostate growth, differentiation and apoptosis, respectively. These will be correlated to transfected cell status, tumor growth and invasive potential.

[0260] There are to be no expected difficulties in making the cDNA gal constructs. Antisense technology, however, can be unpredictable with variable impact on the expression of the sense RNA to any gene of interest. Alternatives include: 1) antisense constructs directed at only 5′UTR and transcription initiation site (Mackay et al., Invasion Metastasis. 12:168-184, 1992). 2) design a Ribozyme directed at the UGS-derived mRNA or 3) design antisense oligonucleotides to the 5-prime end or transcription initiation site to knock-out UGS-derived gene expression.

EXAMPLE 4

[0261] While the prostate cancer cell LNCaP/C4-2 model described above in Example 1 closely mimics the natural progression of human prostate cancer from non-metastasizing, androgen-dependent cells (LNCaP) that are gradually transformed in vivo into aggressively-metastasizing, androgen-independent cells (C4-2), this model represents only one of the model systems used herein to assay for UGS-derived fetal prostate gene function by reducing the protein levels in LNCaP and by re-expression of the protein in C4-2 cells. Other cell systems, however, may also be used in the present invention to assay for UGS-derived fetal prostate gene function, including, for example, without limitation, normal prostate tissue in conjunction with prostate cancer tissue, and early prostate cancer tissue in conjunction with metastatic prostate cancer tissue. The biological sample to be analyzed in these alternative models may be any tissue or fluid in which prostate cancer cells might be present. Various embodiments include bone marrow aspirate, bone marrow biopsy, lymph node aspirate, lymph node biopsy, spleen tissue, fine needle aspirate, skin biopsy or organ tissue biopsy.

[0262] All developmental switches have a role in prostate development and/or diseases of the prostate, including, without limitation, prostatitis, and benign and malignant growth of the prostate gland. Such developmental switches include proteins encoded by messenger RNAs including, for example, without limitation, certain messenger RNAs listed in Tables 1-5. In particular, such developmental switches include those mRNAs which encode proteins including, for example, without limitation: ugs186oft which encodes mus musculus (mouse). protein kinase clk (ec 2.7.1.-) (483aa) or related proteins; ugs 160 which encodes homo sapiens (human). kinesin-like protein eg5.10/1996 (1057 aa) or related proteins; ug381 which encodes homo sapiens (human) elongation factor 1-beta (ef-1-beta)(224 aa) or related proteins; ugs101 which encodes mus musculus (mouse) retrovirus-related pol polyprotei (1300 aa) or related proteins; ug485ors which encodes homo sapiens (human) putative rna-binding protein rnpl (157aa) or related proteins; ug356 which encodes rattus norvegicus (rat), and mus musculus (mouse) heat shock cognate 71 kDa (646 aa) or related proteins; ug108rcon which encodes escherichia coli tetracycline repressor protein class (216 aa) or related proteins; ugs045 which encodes Rattus norvegicus Smad4 protein Smad4 mRNA, complete cds. 4/98 (3041 nt) or related proteins; ug048 which encodes Human DNA sequence from PAC 434P1 on chromosome 22 Contains (45548 nt)or related proteins; ugs225 which encodes Mus musculus chromosome 19, clone D19-96, B7, complete sequence. (769037 nt)or related proteins; ug156rcon which encodes Homo sapiens protein associated which encodes Myc mRNA, complete cds. 8/98 (14807 nt)or related proteins; ug157rcon which encodes Homo sapiens ALR mRNA, complete cds. 9/97 (trx-G paralogue, trithorax gene complex, homeotic) (15789 nt)or related proteins; ug 192rcon which encodes Human FUSE binding protein mRNA, complete cds. 5/94 (2325 nt).

[0263] In addition, such developmental switches include those listed in Table 1, wherein the results of the library analysis of 728 cDNA UGS-derived ESTs are presented using the Swissprot database, including, for example, without limitation: ug517 which encodes mus musculus (mouse). k-glypican precursor. 10/1996 (557 aa)or related proteins; ugs016 which encodes mus musculus (mouse). bone proteoglycanII precursor (p(354 aa)or related proteins; ua2h6f which encodes mus musculus (mouse). insulin-like growth factor bindin (305 aa)or related proteins; ug 130 which encodes mus musculus (mouse). insulin-like growth factor bindin (271aa) or related proteins; ua1a2 which encodes Homo sapiens (human) son protein (son3). DNA binding protein w/ mos and myc homology 11/1995 (1523 aa)or related proteins; ug271 which encodes mus musculus (mouse). carg-binding factor-a (cbf-a). 11(285aa)or related proteins; ug277t which encodes ambystoma mexicanum (axolotl). homeotic protein hox-a13 (107 aa)or related proteins; ug367 which encodes mus musculus (mouse). embryonic tea domain-containing factor (445 aa)or related proteins; ug486 which encodes rattus norvegicus (rat). lim protein clp36. (contains homeodomain of lin-11) 10/1996 (327 aa)or related proteins; ug293 which encodes Homo sapiens (human). ptb-associated splicing factor ps (707 aa)or related proteins; ug485ors which encodes Homo sapiens (human). putative ma-binding protein rnpl (157aa)or related proteins; ug101rcon which encodes mus musculus (mouse). dipeptidyl peptidase iv (ec 3.4.1(760aa)or related proteins; ug211 which encodes mus musculus (mouse). matrix metalloproteinase-14 precu (582 aa)or related proteins; ug335 which encodes rattus norvegicus (rat). neprilysin (ec 3.4.24.11)(neutra (749 aa)or related proteins; ugs044 which encodes mus musculus (mouse). tlm protein (tlm oncogene). 12/199 (317 aa).

[0264] In particular, such developmental switches additionally include those in listed in Table 2 wherein the results of the library analysis of 728 cDNA UGS-derived ESTs are presented using the GENPEPT translated protein database (rel 102.0), including, for example, without limitation: ug135 which encodes breast adenocarcinoma metastasis-associated gene (contains SH3 domains) Homo sapiens (715aa).

[0265] In particular, such developmental switches additionally include those listed in Table 3, wherein the results of the library analysis of 728 cDNA UGS-derived ESTs using the primate rodent GB 103 database, including, for example, without limitation: ugs 186s which encodes Mus musculus cdc2/CDC28-like protein kinase 4 (Clk4) mRNA, comple (1549 nt)or related proteins; ug206 which encodes Rat mRNA for short type PB-cadherin, complete cds. 7/96 (4153 nt)or related proteins; ug392 which encodes Mus musculus vascular adhesion protein-I gene, complete cds.9/98 (14357 nt)or related proteins; ug142** which encodes Mus musculus tumor susceptibility protein 101 (tsg101) gene, comp (33613 nt)or related proteins; ug219** which encodes Mus musculus tumor susceptibility protein 101 (tsg101) gene, comp (33613 nt)or related proteins; ugs216 which encodes Mus musculus retinoblastoma-related protein p130 mRNA (4013 nt)or related proteins; ug414 which encodes Murine gene for interleukin 5 (eosinophil differentiation fac (6727 nt)or related proteins; ug159 which encodes Mus musculus WW domain binding protein 5 mRNA, partial cds. (proline-rich, sh3domain interactive protein) involved in regulation of transcription in development of kidney and limbs. Homologue of Drosophila enabled. (647 nt)or related proteins; ug422 which encodes Mus musculus timeless homolog mRNA, complete cds. 11/98 (4438 nt) 7. le-47 (Mammalian Circadian Autoregulatory Loop: A Timeless Ortholog and mPER1 Interact and Negatively Regulate CLOCK-BMAL 1-Induced Transcription) ugs045 which encodes Rattus norvegicus Smad4 protein (Smad4) mRNA, complete cds. 4/98 (3041 nt)or related proteins; ugs 192 which encodes Homo sapiens protein associated which encodes Myc mRNA, complete cds. 8/98 (14807 nt)or related proteins; ugs213 which encodes Mus musculus dishevelled-3 (Dvl-3) mRNA, complete cds. 6/96 (2498 nt)or related proteins; ugs218 which encodes Human Krueppel-related zinc finger protein (H-plk) mRNA, com (2873 nt)or related proteins; ug281 which encodes Human mitosin mRNA (mitotic progression factor), complete cds. 12/95 (10211 nt)or related proteins; ugs234 which encodes mus musculus high mobility group protein homolog HMG4 (Hmg4) mRNA (1502 nt)or related proteins; ug494 which encodes Human alternative splicing factor mRNA, complete cds. 9/91 (1717 nt)or related proteins; ug088rcon which encodes mus musculus matrix metalloproteinase-14 (Mmp14), exons 9 (1242 nt)or related proteins; ug179rcon which encodes mus musculus ATP-dependent metalloprotease FtsHl mRNA, complete clone (2654 nt) or related proteins; ug380 which encodes mus musculus male-enhanced antigen (Mea) mRNA (human chromo 6p21.1-21.3), complete cds. (841 nt).

[0266] In particular, such developmental switches additionally include those in listed in Table 4 wherein the results of the library analysis of 728 cDNA UGS-derived ESTs are presented using the GenBank database, including, for example, without limitation: ug031 con which encodes mus musculus vascular adhesion protein-1 gene. complete cds. 9/98 (14357 nt)or related proteins; ug059 which encodes Homo sapiens gene for osteonidogen, intron 9. 3/98 (9085 nt)or related proteins; ug039rcon which encodes mus musculus 9ORF binding protein 19BP-1 mRNA, Binding of Human Virus Oncoproteins to hDlg/SAP97, a Mammalian Homolog of the Drosophila Discs large Tumor Suppressor protein (2703 nt)or related proteins; ug051rcon which encodes Mouse mRNA for prothymosin alpha. 6/91 (1191 nt)or related proteins; ug033con which encodes M.musculus TSC-22 mRNA. Isolation of a gene encoding a putative leucine zipper structure that is induced by transforming growth factor beta 1 and other growth factors. 12/93 (1706 nt)or related proteins; ug092ft which encodes Gallus gallus single-strand DNA-binding protein.csdp SSDP (sequence-specific single-stranded DNA-binding protein), mRNA,(1211 nt)or related proteins; ug092ors which encodes fb33f07.yl Zebrafish WashU MPIMG EST Danio rerio cDNA 5′ similar to Gallus gallus single-strand DNA-binding protein. csdp SSDP (sequence-specific single-stranded DNA- binding protein), mRNA (396 nt).

[0267] This comprehensive approach and evaluation as listed above in Examples 1-4 permits the discovery of novel genes and gene products, from among the UGS-derived EST cDNA clone designations provided, inter alia, in FIG. 1, FIG. 9, and as presented in Tables 6 and 7, as well as the identification of an array of genes and gene products (whether novel or known) involved in novel pathways that play a major role in prostate disease pathology. Thus, the invention allows one to define targets useful for diagnosis, monitoring, rational drug screening and design, and/or other therapeutic intervention for prostatic disease processes, including but not limited to, prostatitis, and benign and malignant growth of the prostate gland.

[0268] All publications, patents and patent applications mentioned in this specification are herein incorporated by reference in to the specification to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference.

[0269] All of the compositions and methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations may be applied to the composition, methods and in the steps or in the sequence of steps of the method described herein without departing from the concept, spirit and scope of the invention.

1 811 1 601 DNA Murine misc_feature (1)...(601) n = A,T,C or G 1 gaattcgaag aagtccttca gtatcttcac cagagccaac tgaaaagtca aggtcttcac 60 ggaggaggcg ctcagtttct tctccccgta ccaagacaac ttcgaggaga ggacggtctc 120 cttcacmcaa acctcgtnng actccaaaga tccvgatccc gctcacggag agagaaamcc 180 agaacaancc cgacgcagag atagatctgg atcatctcag tcaacatctc gaagaagaca 240 gaggagccgg tctagatcac gagttactcg gagacrgagg ggtggctctk gttaccattc 300 aagatcacct accagacagg agagttctcg aacctcctct agacgcagaa gaggccgctn 360 cccsgacacc cttgaccagt cggaagcgat ctcgatcaag aacatcacca gctccttgga 420 mgcgctctag atctsgagcc tcaccagcta ctcatsnggc ggtccaggtc magaacacca 480 ctgataagcc gacgtaggtc cagatctcgg acctcacctg tgagtaggag acggtcaagg 540 tcagtgaata ggcgtagatc tcgatcaaga gcatccccag tgagtcgaag gcgatccagg 600 t 601 2 243 DNA Murine 2 gaattcgtta tattttaaaa ctgctacttg tataaatctt tcccaaatac cgtgggtttt 60 gtgcatagtt tttacagata tggatttagc agactgtctt ttcactgtta tgggtttttt 120 agaagttgag catttttatg gctgataaag tgaatgttac ttctaagtgc tcacttcttt 180 tatcagaagt gaccctcagt ccattgtgct acsttagctt gcctctttgg taataatkcg 240 kag 243 3 209 DNA Murine misc_feature (1)...(209) n = A,T,C or G 3 gaattcatcg cacaaaaacc ctggtatgaa gtcactttcc aatggaattc caaagcctaa 60 ggatgaacta tcctgcctga taaaaaccaa cagctggcct gatcgctcag aacacctgtg 120 acatgtcctc cctagamggg acagagtgat agttcatgtt tgnnkgtgtg tggactawyt 180 kgktactacc tttagagcaa ctgatktat 209 4 357 DNA Murine misc_feature (1)...(357) n = A,T,C or G 4 gaattcgggg tgtcctactg actgatattc atttgatttt attcatttgg attcatacct 60 cactgtcata gccgcaaawt ttatttaacc catgnccttb ccmgatgcya ggtgagatct 120 acytrgtgaa cttaawwaam gcagactggg acctaggaaa attcaccatt ttcattgtaa 180 tgttctcggt tttgccttta tccatagaaa agtgggctct tgggaatgat gaggacactg 240 aggggtggag gatacmaacs gaaaagctca tggagataga gtkcaagcag agagtgtggg 300 tgctyaaata ctcaagagat ttaattaagt ctcgctctca awtgctataa gtttaaa 357 5 331 DNA Murine misc_feature (1)...(331) n = A,T,C or G 5 gaattcggcc aaggccttgc cagctgctga aactgagaag gaagcggtgc cggtcccagt 60 gcaggaggta gagatcgatg ctgctgcaga cttgagtggg cctcaggaag tagagaagga 120 ggagncccca ggctcccagg accccgagca cacagtgacc cantggcctg gnagaaggcg 180 gaagctccag gracmgttag cagtkctgcy kdarggscnn yaaggamcct ncyygtkcyc 240 cccanggatt cagngagnca gttccagara aaatyctgta cagtktacac acggtgtsca 300 tatcgtggag aractcacat ctctgtgcgc g 331 6 331 DNA Murine misc_feature (1)...(331) n = A,T,C or G 6 gaattwgcaa agaaaccttc tttaaaatgg actcagaaga tgggtgtagg ggcgttgcca 60 atgtggctga gttctgtgtt tggaaatgtg ttgctgatgc acatgatgaa agaagagccc 120 agatgaccct aactcttcag gaaawdcaac catctatatc agtcttatct ctgctctcaa 180 aatgctctca gagagtaaam mmaaatggcc cttnggtata cnyctctccg ttttgttttt 240 ttaaagrwtg cctagkaatt tttnaaaaag kgcaaaagrt gtktyytgag atttyctttt 300 yaattytggg tgtcagtgtg tgdgtgtttg t 331 7 427 DNA Murine misc_feature (1)...(427) n = A,T,C or G 7 gaattccttg caggchgcct gvggkvcnac cnttctgaga gccagaaaac tgctctcagn 60 tacattcctg gcagctcctg accctgagcc tctattcaca ttccttcaca aaacggccca 120 ggctcaaatt gaaaaggaaa taaaagagac cacaataaaa ttgctaacat acggagtaac 180 agagtgatct gtgacacaat tctgctccat gttttccttt cccttcaagg acagctgggc 240 agccactgag gcctgtggac aaggatccat gatcatttcc aatgttcaga gagtccagca 300 accaccaggc aagggctgtt ggcacytagg aatgggtctg cttgcatgtc aagggaccaa 360 tgtggtccta caaaactcat ttctactgaa atgtcatctt ctgaachttg ggaaataatg 420 cmctaga 427 8 520 DNA Murine misc_feature (1)...(520) n = A,T,C or G 8 gaattccggc cgtgctccgt ccttcgctcc ktgtyccgtc asrcactgtg agggstcagc 60 gwgaggtcgg tggggttagg naacgcggcg gcggcggcgg cggcggcggc ggctcctcct 120 ccnaagatct gagcagggtg ccagaacagg natgtacacg ctgctttcgg gattgtacaa 180 gtacatgttc cagaaggatg aatactgcat cctgatcctg ggcctggaca atgctgggaa 240 gacggtaggt ccctgctctc tcaccagttc ccattccctg cctgatctaa ncccccgccc 300 caaggctaca ggttagtagt caccagcctc ctgaagatca agccacaggs agaggcgtgc 360 atggctgcat ngggtgtgaa gggataggtg ggaaggacac cagaaaacta ctctagctgc 420 tgctatctna mccccctctc tttttttcct cagactttcc tggaacagtc aaaaacacgc 480 tttaacaaga actacaagga attccaccac actggcggcc 520 9 465 DNA Murine misc_feature (1)...(465) n = A,T,C or G 9 gaattctgtt aatgcacctc tgcctccacg gaaagaacaa gaaatgaaag aacctcctta 60 ttcatctggc tacaatcaaa attttacttc atcaagtaca cagacagtat cccaatgcca 120 gctcccagct gtacacatag accagacaac tcagcctcca gagactggta tgacctctgc 180 atatattctt tataagtacc acatgccaac ttkgtgcttt actggagtac cctctatags 240 ccytctgaaa acttagacag kagcctttca agkaaacart ctgtagtgcc cytacarctg 300 traatactta tctctttaat gtnttgtctg gkagaaagac attttgatgt attttcctcc 360 atttagttaa gtttacctct agtggagaat tagttaaacc actttggctc ctgaagggtc 420 tcatgtgcat atgcgctgta ctctyccaag agcdntgtgg attct 465 10 541 DNA Murine 10 gaattccttc ctgtaaggct acttttcttt tttctacttc cttttccagc aattcatagt 60 taggcttttt cctggtataa agtctaagcg tctctatgca gatttcctga atctcctctt 120 ctgtggtacc aaacagaaga aaccaatggg gccgagttgg caagggaatt tgaagtgctc 180 tagctgcaag gtagatacaa gcacatgcta tagtctctgg ttgaaagcga acaaagacat 240 tggttcgaag actgtcattc atgtaattcc aggcagtttg aaccagggtt tggttacgtt 300 cacattctaa gacttgtaaa tacattacaa tgatcttatg gggatgcttg acatgaacac 360 aaaatcccaa ctcctttagc accctcctct ctgccttgat aacttgattt ttggtgttaa 420 tgtagttctg atcaaggatc agggggcttg gagtccyttt tccycttaac tggcggaggt 480 ggtggaatac attaatcaca tctctwattc ttyttggcgc ttcttcgatt tttgacscaa 540 g 541 11 330 DNA Murine 11 gaattcgctg cgtcgggcgt gcgtggagct cgctggaact atggcgtccg ggcctcaccc 60 gacctcgacc gctgccgccg ccgccgccgc tgccgcctcc gcctcgtccg ccgccccgag 120 cgcgggcggc tccagctccg gcacgaccac cacgacgacg accacgaccg gagggatcct 180 gatcggcgac cgcctgtatt cggaggtgtc gctcaccatc gaccactcgc tgatcccgga 240 ggagcggctc tcgcctaccc cgtccatgca ggacggcctg gacctgccca gcgagacgga 300 tctkcgcatc ttgggstgcg agctchatcc 330 12 330 DNA Murine 12 gaattcgctg cgtcgggcgt gcgtggagct cgctggaact atggcgtccg ggcctcaccc 60 gacctcgacc gctgccgccg ccgccgccgc tgccgcctcc gcctcgtccg ccgccccgag 120 cgcgggcggc tccagctccg gcacgaccac cacgacgacg accacgaccg gagggatcct 180 gatcggcgac cgcctgtatt cggaggtgtc gctcaccatc gaccactcgc tgatcccgga 240 ggagcggctc tcgcctaccc cgtccatgca ggacggcctg gacctgccca gcgagacgga 300 tctkcgcatc ttgggstgcg agctchatcc 330 13 530 DNA Murine misc_feature (1)...(530) n = A,T,C or G 13 gaattcgggg ggtcttcctg ctcttgaagc actgggtgga acggggtccc agtagccgca 60 ctcagcctta gggtctgcat cccattaggt ttctagggct gcaggggctg caggaccang 120 ggccatgngc tccntncact tgaccctgca gctgggtgtm aganagtcct gtknggttcn 180 cacctymagg ggatgtycct accmacnttn cacctkctca agnctycact gtctggggcc 240 tgtgngctct cncaacagct tcttccttcc tttgcccttc gtgtcagcca gcagccttgc 300 caagtgtttg ttwatttwat actttgtgnt ttttgagaca gtcacatcaa ggttgaactt 360 agaacccaag atccnyactg ctatcacccc ctgaatactg gggnttccna gngtgtnnnn 420 cctgggntcc manncctcag gacnacnnnn cttasvnnag gatanccgta tcacgtnctt 480 gggsnccatc ccttttttcc ccactacana gdaagnnnnn ncccgawytc 530 14 537 DNA Murine 14 gaattccttg ctgtgacaca ttttttctag taagtgttac tctttcaatc aaaaccccta 60 taccaatgga gcttaattta ggtagtgaat tagttcctaa atagatcagt gattgtgaac 120 aaggcaataa aaagaaaacc tctaatggta tcaagtgttc ccataagtac tttgtataca 180 tgtggatgtg tgttggtgtg catgcacata tgtgtgcatg tgtgtggatt gcgaaggaca 240 gcctttggtg tcattcctca ggtggtgtcc accttgtttt gaagagatag gagtgtcaca 300 ctgaacctgc agcttgctga ttcagagtac cagggacatg cctggcttga cctctccaac 360 actgggatca caaggaactt tcgtcagcag gtcttgchtr kwtgaaatag ttgagaggga 420 ctgcactccg atcttcacac ttgcacataa tgcatattgc caaatggccc atctccttga 480 ctccactgaa taaaattttt gactaatttc tcaaaataat tacagcagcc tgaattc 537 15 302 DNA Murine 15 ggaattccct gcctctgtaa ctccttbacc caattcttag cccgtgcaaa tgtatctgtg 60 ttggtgatgt catagaccac aatggctgct tgggcccccg atagtacatc ggggccaggc 120 tgtgatagck ctcttggcca gctgtgtycc agatctcaaa cttgaccgtt gtatcgtcta 180 agcagacagt ctgtgtgagg aaakttgctc caattgtgct ctyctggtac tcatggaact 240 kccccttkac maagcggagg dccaggctgg actttbccac ggcagtytck tccaagaggd 300 cc 302 16 312 DNA Murine 16 gaattcgtgg aagccccggc ccaaagtaac gctgctgccc ggagccgcgt tggaggcctc 60 ccttcccatt aagtygcctc tttagcatag caccggcccc acccccacgs tcactggtac 120 tactacagag cagckcgcca tggcgggtcc gaggaggtgc agcacgaacc caatggacca 180 gcttgctggc aacaagatct tgtcagttta agcttggkcc tcttygggcg agtctkccgt 240 trggcaagkb carcctggty ctcccgcttt gtcaaggggc agttycatga gtaccaggag 300 agcacaattg ga 312 17 310 DNA Murine misc_feature (1)...(310) n = A,T,C or G 17 ggaattcgcc gctttttttt ttttaattca aaacatttga ctttttaaag gaaaggatgt 60 cacagtgtct ttataaccga gataatgaaa tcttagctta attttgtgca agaattaagg 120 tacttgaatt gattaaggca cagatgtgtt tggtctaaaa ggctgtattt tgtctgcttt 180 ttcacaaatc tatggaaatt gatttcccca tcttgcagtg tgcttagckc ccacgntccc 240 caagttctag aattctggaa agadccttca tgtatggaat gtcttctgtk cagaggaggt 300 nctcagcata 310 18 392 DNA Murine 18 ggaattcctg acatctgatc aggagtaaac agcacacaaa gggagtgttt taaaggttty 60 ctgcagtgtg aaacaaactg tgtctaagta caagggctct ggaattacaa agtttacaaa 120 gcagctctac cacgtctcca aggccaaaat agatgcccgg aagagggaaa ggggcaagag 180 agctgtccga agcagtacac cagcttaagt gacatgaaat aacttggaca aggttcaaac 240 tgagagactg cagttgagat gaagtgggaa aaaatattgg aattcagtcc aatagagttc 300 acagaacacc accttaaycc tgcatccctt bccaaaatgg aaacaaagtt gtwtcaaaaw 360 mtccagttca tccaaggaat ccaaacatsc tt 392 19 148 DNA Murine 19 ggaattcaaa tagtggttgt yctttagatg gaagatgtga gtcaaagcca aggtcgctct 60 ctctggaagt cagtgagtag cagggaccag agcgtattgc tgcagtatag actgaacgga 120 aggaaaacca ctgcycaggg kgccgkkg 148 20 382 DNA Murine misc_feature (1)...(382) n = A,T,C or G 20 ggaattctcc gaccgtgcgg acttaagatg gaggcacttc ctgtctkcgg cgggaagaga 60 aggctcggtc ggagccggga atgctgggac ttgtacgtcc gccggtcacg gccgcygccc 120 ccagcgacgt cacccacacb ngcagaagcg gacgccgcgg tcaagatgtc tctgccatgc 180 ccacgggacg cacggacgca cggacggacg gacggactcc acaaggkagg aagcctgcbc 240 cggagcgcac cggbcgcacc caccacagca cacaggacac acgcgggccc bbsccccgcc 300 caggcacacg cggbacacac ggcacacacb ggcmaggcag gccaggscac mcgcayckcc 360 aggaccccbc ctgcgmcccg cc 382 21 166 DNA Murine 21 ggaattcccc ggctcgagcg gcgctttttt tttttttttt ttccatttca actgcaattt 60 tattgagggg gacatgtctg tacgcagtca ggccctgttg gcgtgctcct tcctccgtga 120 gaabcgctyc gttctgkkcg gcctcdgcgg actmcgcgca ccttgt 166 22 206 DNA Murine 22 ggaattcgct gaccgcatgc agaagccacc acacttttat acaggtttat acagcgtykk 60 caatcaaakc ctagacaggc acctacaccc aakcttcaaa gtatttttaa aatkkccaca 120 aaattcaatt cttwggaatt tctcttagac actgttcaat ttaaattttt tkcaatkggg 180 acagaacctg gggctttgtg tttgtt 206 23 305 DNA Murine misc_feature (1)...(305) n = A,T,C or G 23 gaattcctgg tgtacactcg aawttkbttg rgvmmaaagg agaggactcc aacaaaaggt 60 tctaaatgct gtttgaaakc tgccagggtg attctcttat caacatgcac catcaaccat 120 ttgtgtcctt yyycagagcc ttcatcckcw gbtgtagggg tcnkctttga agtacatgta 180 ctgcatgtyc cccctttttt tkbcactctc ggtcatattc actgtcagtc ccagagtctt 240 cttywgctgt gtyccaggkc tccytttttc cctcggttgc tttagktctt ctactacytg 300 tgact 305 24 288 DNA Murine misc_feature (1)...(288) n = A,T,C or G 24 gaattcgttg gwktnmtctc ctctcacttc aaggttttaa atgctgtttg aaagctgcca 60 gggtgattct cttatcaaca tkcwccatca accatttgtk ttctttycca gakccttcat 120 ycgcwgtgta ggkgtcagct ttgaagtaca tgtactgcat gtcccccctt ctcttkcyac 180 tctyygttca cattcwgact tctgwtccag atwwctttcw gtcygagggw cttytctktc 240 tcagatgtga atwwatgdty sgagtacaag gttckggtag acaggtga 288 25 249 DNA Murine misc_feature (1)...(249) n = A,T,C or G 25 ccagctcagg aagagcctct ccacacgggt caaagggcat ctttgatcag aagccttctc 60 aggtkctctt gtyctgctct ggdgtycctc agctgtctgc agcwcccacc agacactgtc 120 cattgctgtc tgccatgctt gtctttatgt cgtgtgtttc tcgtccctra vttcaaccta 180 tkcncccttt cctaacaaca tgactacctc atktytnctt cagaccatag tgkgacccct 240 rggttccca 249 26 288 DNA Murine misc_feature (1)...(288) n = A,T,C or G 26 gaattcgtta tattttaaaa nctgctactt gtataaattc tttcccaaat accgkgggtt 60 ttgtgcatag tttttacaga tatggattta gcagactgtc ttttcactgt tatggggttt 120 tttagaagtt gagacatttt tatggctgaw waargtgaat gktacyttct taargtgctc 180 aacttctttt atcaggaagk gaacccycag ktccattgtg gcyaacgtta ggcttggcct 240 ctttggtaat aawtgcgtag btctygkatt gaacngctag gattaggc 288 27 355 DNA Murine misc_feature (1)...(355) n = A,T,C or G 27 gatttcgaga ggtggtccct cggatggctc tccctgctca catccggaag ttcaaatatt 60 gatgcttcch cccccccccc ccacnnbtcc agactttcat tttctctccg gtttggacac 120 aagagagaga gagagagaga gagagagaga gagagcgcta cagaagttgt ttacaaacca 180 gagaactgtt cattaagtga aaacgttagg sagcacatgt tccgcagaag ataacaaaat 240 agatggsgka aatagtgtag tcggtgtcga agcaatatta awctdtkcct attcccvgct 300 aaataaagtk aagccaccga ttttttgttt ttgagatctc tatggrkgta tggag 355 28 391 DNA Murine 28 gaattccccc agaaaatata aggatgccat acactttata attctaacac cattgattaa 60 aaaaaaaaaa aaaggaaaaa atgctgccat tttaatggca ttttctcatc aaaatcaacg 120 tgtgcttttc atatttcaaa ataaggcatt atatgctatt tcaaaaaaaa atttaagacc 180 aaaagtacat gcttactttt agaagcatgt acatttttta aaaaggatct attcagttag 240 caaatgagtg ttgtgaagag ctgctcacta aaagctaact gtagttaaaa ggttatatag 300 tggcattttc aagtgacagg aaattcaamt ttactttttc caaaggattc cacaagtgca 360 gtagtgcact agtgtacccy sctgaagtct g 391 29 276 DNA Murine misc_feature (1)...(276) n = A,T,C or G 29 ggaattctcc gaccgtkcgg acttaagatg gaggcwcttc ctgtctkcgg cgggaagaga 60 aggctcggtc ggagccggga atgctgggac ttgtacgtcc tytkgtcack kbykcnsccc 120 ccagcgacgt cwcccacack kckcagatty sgactyygck gtcaagatgt ctctgccatg 180 cccacgggac gcacggacgc acggacsgac ggacggwctc cacmarggta ggaagccttc 240 ttcgakctba mcttygstwc caacacagca cacagg 276 30 330 DNA Murine 30 ggaattccat gattgttgaa ctactgggtc aaaactcaaa tgaggtgaat ttgcctttaa 60 aggacttact tatgctaaga accaactaat agccgtgaga caatcacgtc atagctacca 120 gtacaagtag agcaaatatt tatccattta gctctgagct ctatattata taatggagcc 180 ttaaatctat gtggttttta tcaatggttt gtcttttgaa tggttgtgga aactgtagat 240 aaccttaacc aaggactgta caaacgtgaa ggtgtggtct yacwcttcag gtttaaagtg 300 tttgadgcat tattagcawt cattcacaac 330 31 455 DNA Murine 31 gaattcaaaa tatttctttt ctgtctcaaa agctattatg tcccattttg gggtgttttt 60 tagctctacc tcagaaaaac aaaagaagaa gaaataaaaa ataaaagtca agaacgaacc 120 ctgaatttct aaggcttcca tccaatactt cttaagctaa gttaagattg aaattctttc 180 tcaggctaat gctgtgtgaa gcaaacaaca ctcacattta gagcaagcat aatttcaaga 240 gatgccaaat ccaagttcaa aagcccacca gaggcagcgg ccatggccat gatgaataca 300 aagcatgaaa aggtgtgtct gtctccaggc ctctgtgaca ggaaaactgg ctggctgtyg 360 cagtcagtta aataagtctc acttcaagct ctkkbbcaga gccttctacc ctgctagact 420 gttgctaata taaacamgta gttctgtgtc gtgta 455 32 460 DNA Murine 32 gaattccaaa aaattattta aaawaaaaaa aagttctttt gatctttccg tacagtattt 60 tagttgaaga ttagaattcc tttctctttg agaaagcaaa agttcctacc ttaacatctg 120 taaaaaggaa ataagaggcg cccaaggctg taggctctaa ggaaatkgcc gtagacttca 180 tcacagggca tctttgwtya tccagcaggg agttctgagt aggccaggct tctactaaag 240 ctgatttctg tgacctttta gatggggact gtcacctcat taaacatagt cacctttgkt 300 ttgaacagga aagttggtgt ttgtttgttt ktttttaaga cagagttgta ctgktatagg 360 cakkgbtttk ccctgagtta actatgtaga ccwggctagt gccaaactta tcaaaatcta 420 tctakctytt bcyctwgagw gttkggatta arggtgtggg 460 33 375 DNA Murine 33 gaattcggag tgcttatgtt tgagatgatg gcgggaaggt ctccgtttga tatcgttggg 60 agctctgaca atcctgacca aaacacagag gattatctat tccaagtcat tttggaaaag 120 cagatccgca tcccgcgttc tctgtctgta aaagcagcaa gtgtactgaa gagttttctc 180 aacaaggacc caaaggaacg attgggttgt baccctcaaa ctggatttgc tgacattcaa 240 ggacatccat tcttcagaaa tgtggrctgg gacatgatgg gkbaaaagca ggtggttcch 300 ccctttaadc caaacatttc tkggrgaatt tkggtttgga taawttcgat tctcagttta 360 cydatgaacc agtyc 375 34 502 DNA Murine misc_feature (1)...(502) n = A,T,C or G 34 gaattccttg ggaatgaagg gcggaatgtg gctcagtgtt gagtggtcaa agtgtcccag 60 tgagggagaa gtctggagaa gggcagtggt gagacctgma amcctgaaag cagctgcact 120 gtacacttca tggccraagc atcaatcctg agtatgctgt cacatgttaa aacaactgta 180 cacattgaga caagcagaag tcacctgact ctctcagtgg gacagtgctt ctccwctcac 240 gccactgtac tgactgagga cggatcccac gttgggctgt ctgcctaaan tccanyttgg 300 rcmgcacacc ctgaggagca ggcaggcang gctctgaaag cagagcatga tccagtcaag 360 gctcaggsag cytcacahnn ctgaagraat catcagagtc acacttccct cgtgtgtaca 420 accaggaagg aggatgctgc atgaacgcac tgagaattca ttcagtgaga ctctgagaaa 480 agagcctgac acgtcgaatt cc 502 35 496 DNA Murine misc_feature (1)...(496) n = A,T,C or G 35 ggaattctct ttgcatagag gtgcagccct gggcggcccc gchdhkhhhc tcctccacgt 60 cctcggggac cctggtctct gctccctcct cactattgaa ctcagagcta ctgggggaaa 120 gaatgcaggt tggagaaaga ctccagggag tccaagctgg gcgagtcccc aggggggctc 180 ggctcgctgc tatcccaacc cgggctccsa gctgcccctg aaggcgcttg tcacaggcgc 240 gggtacctgt gaaaagagac gcgtgggcac caccccacag caggttgcag acagtgatga 300 cgaccactct gagggagbnc tggtggagaa ccacgtggat gggaccatga acatgttggg 360 aggbbgtagc agtgctggch vgaagcccct caagtcaggc atgaaggagc tggctgtgtt 420 ccgggagaag gtcaatgaac agcaccsgca gatgggcaag ggtgccaaac acctcagtct 480 ggaggvgccc aagaag 496 36 424 DNA Murine misc_feature (1)...(424) n = A,T,C or G 36 ggaattcttc cttcctttaa tcttaagtaa aagagacaca gggattcaaa aataaaaatt 60 tcttnnccat tcccaggcct gtacccagtg ccctccatac cacccttncc ctctctaaca 120 gaagcaaggg aggttcagct taacagccgc tggggggggg tcagangggg ggcttctgag 180 ctcagtgttg gtctctttcc aaatataaat acatgtgtca aaactkggga actcctccac 240 acccgtcacc ctgannccct ccatttctgc tggtgttcgg gatgggggaa gccaggcacc 300 gactggctgg gvgtttactg cacactttgg ggcatkgggc cccaccagtc tcctgcygct 360 cgttdgtagv aagagatggs acycvggggt yhhccccgga twggtkggga ggctccctgg 420 atgg 424 37 496 DNA Murine misc_feature (1)...(496) n = A,T,C or G 37 ggaattctct ttgcatagag gtgcagccct gggcggcccc gchdhkhhhc tcctccacgt 60 cctcggggac cctggtctct gctccctcct cactattgaa ctcagagcta ctgggggaaa 120 gaatgcaggt tggagaaaga ctccagggag tccaagctgg gcgagtcccc aggggggctc 180 ggctcgctgc tatcccaacc cgggctccsa gctgcccctg aaggcgcttg tcacaggcgc 240 gggtacctgt gaaaagagac gcgtgggcac caccccacag caggttgcag acagtgatga 300 cgaccactct gagggagbnc tggtggagaa ccacgtggat gggaccatga acatgttggg 360 aggbbgtagc agtgctggch vgaagcccct caagtcaggc atgaaggagc tggctgtgtt 420 ccgggagaag gtcaatgaac agcaccsgca gatgggcaag ggtgccaaac acctcagtct 480 ggaggvgccc aagaag 496 38 424 DNA Murine misc_feature (1)...(424) n = A,T,C or G 38 ggaattcttc cttcctttaa tcttaagtaa aagagacaca gggattcaaa aataaaaatt 60 tcttnnccat tcccaggcct gtacccagtg ccctccatac cacccttncc ctctctaaca 120 gaagcaaggg aggttcagct taacagccgc tggggggggg tcagangggg ggcttctgag 180 ctcagtgttg gtctctttcc aaatataaat acatgtgtca aaactkggga actcctccac 240 acccgtcacc ctgannccct ccatttctgc tggtgttcgg gatgggggaa gccaggcacc 300 gactggctgg gvgtttactg cacactttgg ggcatkgggc cccaccagtc tcctgcygct 360 cgttdgtagv aagagatggs acycvggggt yhhccccgga twggtkggga ggctccctgg 420 atgg 424 39 160 DNA Murine 39 caggaaatrg gacagtctcc aggckycaga ttggagggag crtaccatca cttgttgcat 60 ggagtcccct gtkcctccgt ggggctcagg tkgkaagctd gcccctawgb cwgagcattg 120 bcccattcct cygggggtrg gasctcsawa tbybgctttm 160 40 533 DNA Murine misc_feature (1)...(533) n = A,T,C or G 40 gaattcggcc tgcacagact tctgggatgg cgctgacatc taccctctgt cgggttcaga 60 cagaaagaaa gtgctggact tctaccagcg agcctgccta tccggctatt gctctgcctt 120 tgcctacaag cccatgaact gcacgctgtc ctctcagctc aacggcaagt gcatcgagct 180 ggtgcaggtc cccggccaga acagcatatt caccatgtgc gagctgccca gcaccatccc 240 catcaagcca aacaaccgcc gcagcagctg ghgctccgat gaagggatcg gggaggtgct 300 ggagaaagaa gactgcatgc aggccctgag ckgtcagatc ttcatgggca tggtgtcctc 360 ccagtaccag gcccggctgg acatcgtgcb cctcatcgat gggctggtca amncctgcat 420 ccgctttgtg taccttctct ttggaggatg agctcaggag caaggtgttt gcaaaaaaaa 480 tgggcctgga raaaaggctg gaamtbccam atctcyctmh mbccaaccgg tga 533 41 512 DNA Murine 41 gaattcaaaa tcactaacaa ccataaaagt aaaaacccct tgagaattaa aatgaacgaa 60 aatctatttg cctcattcat taccccaaca ataataggat tcccaatcgt tgtagccatc 120 attatatttc cttcaatcct attcccatcc tcaaaacgcc taatcaacaa ccgtctccat 180 tctttccaac actgactagt taaacttatt atcaaacaaa taatgctaat ccacacacca 240 aaaggacgaa catgaaccct aataattgtt tccctaatca tatttattgg atcaacaaat 300 ctcctaggcc ttttaccaca tacatttaca cctactaccc aactatccat aaatctaagt 360 atagccattc cactatgagc tggagccgta attacaggct tccgacacaa acttaaaaag 420 mtcacttgcc cactttcctt ycacaaggga ctccaatttc actcaattcc aataccttga 480 ttawtatttg aaacaattag cctawtttat tc 512 42 711 DNA Murine misc_feature (1)...(711) n = A,T,C or G 42 ggaattcgtg taagaagcaa gagagagaga gaaagagaga gagabayaya bnyanyanya 60 nymnymnyab mhwgmrdsag nnnnnnnncc tgnnmcagnc catncagggg nntttttttt 120 tttccnactt nagnancaag ntggnnctgn cttnctnncc aaactccnna ggnkgnnttt 180 atttnaaggn ctgnaagntc ggntgncctn cgncccnntg nnttcnaccc nnaggnncca 240 agnaagnacg ntcttnctnc tgntntnccn actctncnac antaagnncc ttnncatttn 300 nagncaagnt ccntggnnaa ctcntctnat ngcttnngcn agncagnctn ctncccnntt 360 nccccnacnt gntgntncca gnscanccat ncgtcctaag gtcatctcag cagacgctgt 420 acgatgagca cacagtcttc cagtgaaatc cgccgtgatg gtgatgagca gcatcctcgt 480 gagaggagat tgattttgtg gttactacgg agcttctcca agagaaggat gagtacagga 540 taggcagagg atgcctctgg gaccctcggg gtacatggca ctcacacctc tcattgctgt 600 gacaggacac ctgacagaaa tgaccacgtt tcaaacatgt gagccttttc aggacatttt 660 aatagcaaat aatgtkggaa taggacatta aatggtaggg cataaacaga a 711 43 455 DNA Murine 43 gaattcctgt gctttccact gtgtggctat tggggggaag tgctgtctta agacattctg 60 atgtttctta ccaggtttgt tttcttcaca gccctaggac tggacaagaa cagagtcata 120 gaaactgctc ctctcagttt ccgaagcctg ctaggtgtac ttggtattga agctgctcta 180 gacagcctga taagattgtt cagtggagat aacaactagt ctcccgcygg caaacacaca 240 ggaacattgc tgggctgagg aacattcaaa atatgttgac tatgagcatt tctcttttcc 300 aattagaaac catatccttc agacatgagt ttgtgtgcat tagtggtata ttacatatga 360 actcccatgg cataaaaaaa aatmmagcta ttaagatatg ttaatagtca acatattttg 420 aatgttcctc agcccagcaa tgttctgatg tttct 455 44 225 DNA Murine 44 gaattcgtga cacatcctta tgaaaagyaa gggggtagtg ctgtcactca catgccagtc 60 gctaagaata agcagtaact aggaattatt gagaagtgca awccywgtat thaatcagyt 120 ctkaatctwc agagccttat agcmaacwag aawwgcywgw ayctgtagca acttgggscc 180 acwkatkggt aggwccwyyg tagtaacaag agaggcacac acttt 225 45 368 DNA Murine misc_feature (1)...(368) n = A,T,C or G 45 gaattcgttg tataagtcac aaaaatctat gatgaaaata aaacgaacaa acaaaaagaa 60 gaaaagaaag agaaaaacaa aacaatactc caccacatta ttcattctta cagtgaatac 120 ataacttcta agtccatcct aagtgtggct ttcttcctat actgcatcca tcagatgttg 180 ttgcatgtct gttagtccta aaatgaactg acaaatatgt cttctctttt tcagaaattc 240 agagtgaggt gtaaacatga gcagaatagt ctttttwaaa ttttttacct taaatccttg 300 aaggtatctt gcagttcacc ctcctgcadg gtcagtgtta gaacctttta atngctatmc 360 accatagg 368 46 376 DNA Murine misc_feature (1)...(376) n = A,T,C or G 46 tgnntcgatg gatccatcga ggcttgcctt tgttgccttg ctcacctgtt gattgctata 60 gagtccctgg ggtccaggaa cctgcaagag atgggggtga aggcctccta tgcataggtt 120 ccatatcamg tgtgttgctt gcctggtggc agcccacayt ttgtacccac ttcctctgct 180 ggctctagga gcctggaaca tgctcttccc cagcctgcct ctggctttcc ctgtggtcct 240 actccgtgcc acagcacytg ggaagtcttt gtgtactaag tctcctgata gccagtkstg 300 ctttagartg tggccgctyc ccaccgctkg ccgggaccat ccatttcttc ttccttcttc 360 caggaagttg gagata 376 47 650 DNA Murine 47 ggaattccat tatttaaaat tattaaccac tcattcattg acctacctgc cccatccaac 60 atttcatcat gatgaaactt tgggtccctt ctaggagtct gcctaatagt ccaaatcatt 120 acaggtcttt tcttagccat acactacaca tcagatacaa taacagcctt ttcatcagta 180 acacacattt gtcgagacgt aaattacggg tgactaatcc gatatataca cgcaaacgga 240 gcctcaatat tttttatttg cttattcctt catgtcggac gaggcttata ttatggatca 300 tatacattta tagaaacctg aaacattgga gtacttctac tgttcgcagt catagccaca 360 gcatttatag gctacgtcct tccatgagga caaatatcat tctgaggtgc cacagttatt 420 acaaacctcc tatcagccat cccatatatt ggaacaaccc tagtcgaatg aatttgaggg 480 gggcttctca gtagacaaag ccaccttgac ccgattcttc gctttccact tcatcttacc 540 atttattatc gcggccctag caatcgttca cctcctcttg ctccacgaaa cwgggtcaaa 600 craccccaca gggtttaact cagatgcaga taaaattcca tttcgcccct 650 48 327 DNA Murine 48 gaattccggc ctttttttaa ggtgtaggga ccacgtgcaa atttcagcac agaccacagg 60 ttctaggagg ctctcttcgt aagttatatc gtctttcaag aaatatcagc caaaagaaag 120 tggtttatta tttttctact tttcttgaac ttggtaaaaa aaatagccat ctctaaatac 180 taaagtattt aagtctcaag ttatatcact tggtatcact tctgtmctgt gtttcttttc 240 tttatmccca cccccttgtt gtctgggagg ccatatgctc atkctgccaa cdytggtcct 300 gtgttaccag gctccagtgc tcctctt 327 49 297 DNA Murine 49 gaattcagaa ggtcctttat ccttccctca agcaactctt ggtttcctgt tagatcctaa 60 ccctgatctt mtcagcagct gtctgtcagg cagtctccac cctgaaccac cttctgamct 120 ctygccatct tttgcctaaa catactattt mctttggggg actaaggtta tgaactgagg 180 gggagtggsc ctaggsccct taaggtaggc cttctwcggt tctggggact aagaaaacca 240 gaacttycct aagytgcctc tggvaagcct aaattccsst atgctccccc caaagca 297 50 160 DNA Murine misc_feature (1)...(160) n = A,T,C or G 50 ggaattcacc accaccacna ccttcagctc atcggatgta cagtttacag ttgagtaaca 60 gtgaacggaa ggattttctt tcttggtcgg atgtgcagaa cttgggatgt gtatatataa 120 atatataata trtataaata tatdtaatnc ngacttaaat 160 51 532 DNA Murine misc_feature (1)...(532) n = A,T,C or G 51 gaatwcgttc ccatgtagga ggtaaaacca attctggaag catctnannc ttccataaat 60 aactttaatw yttagcataa tdacngcctt ngattgtctg nanctcagta gctattaaat 120 aacatcgagt aacatctgca tcaggchctc agaatataca gttgagttgg gagtaaactg 180 aaaagacaaa tgtgttgawg dctatgccan gggaatctnd ctcaaagcct aacacagnad 240 dcancttcat cccagtgacd atnytggacg tacagatggt gatdgcaaag gtgtagaaca 300 cattttttca aagactaaat ctaaaaccca gagtaaamat ccgatgctca gagttagcat 360 aatttggagc tattcaggaa twgcmgagaa atgcattttm acagaaatca agatgttaww 420 ttttgtaaaa chawawwcac ttagamaact gtgtttcatt tgctgtaawc agtttttaaa 480 agtcaratgg aaaaagcaac tgaagttcct tgaaaataga aaatgtaatt tt 532 52 467 DNA Murine misc_feature (1)...(467) n = A,T,C or G 52 gaattcgcgg tgtggaggct ggtgctgagg cgcgggctgg gctggcgaag gttggtgact 60 tgtgtgcagc cagtgaggcg ggtcacctgc angggggcct tgaatgaagg ctgctaggcg 120 agatcagtga agaaggaagg ggcttgggtg gcggaggccg gggagaatca tggaggaaag 180 accngggbnn nbaggctgat gggsgggtta ctgtagaagc tgtccgagga atctggagaa 240 angggagacc ttngtttaga ccgattttkc aaancactgc cccttgttgg agctaccccc 300 ccaaaacccc tgdngdgccc ctgctaccga caatgggcag cctctgttgg atgctccctg 360 tctgtccaag ctctgaccat ctctatatct agtgcttgta cctaggtctg cctcactcat 420 tgaatggagg aatgtttcca gagtagggcc aggtcttctc aaagtgg 467 53 344 DNA Murine misc_feature (1)...(344) n = A,T,C or G 53 ggaattcgtt tcataatatt tattttttca tttgggaact ggggatattt atttaggaag 60 gatggttcag ctcttttaaa tctttgggct cactgatggg gtggggggtg ggacacgggg 120 ttgaaggaac ttgaaagtgg ggaggaatgg tactattggc atgggggtac ctggtattga 180 aaatggacac atnhncyagc tgagagtgat gtcacthgcc tgtaaaccca ttattctttg 240 ggatgctgag gcaggaggat tgagagttag ggactaataa tnrctaggtg ctgacagtag 300 aacaggaagg agggtagaac ctgagttttg tngcctcttt taaa 344 54 402 DNA Murine misc_feature (1)...(402) n = A,T,C or G 54 gaattcggag acgctatncc gcttccatcc gtmdcdcaga ccctgccgga gccgctgccg 60 caatggatga tcgggaggat ctggtgtacc aggcgaanst ggcagagcag gccgagcgat 120 acgacgaaat ggntggaatc aatgaadraa gtagcaggga tggacgtkga gctgacagtt 180 gaagaacgaa accttttwat ctngttgcat atnaaaaatg tgattkgatg ccagaagagc 240 atcctggaga ataatcagca gcattgaaca graggaagaa aacaagggag gagaggacaa 300 wttaaagatg attcgkgagt taccggcaaa tggttgaaah ctgagbytca agttaatctg 360 ttgtgaacat tctggatgta ctggacaaac acctcattcc ag 402 55 525 DNA Murine 55 gaattcgaga agacttacag tggtggcctg ataaggtatt tgggaaaagt ttataccttt 60 cattagagtc ctaacaacca ttcactccat taaatgtttc tgtttgattg aatgagactt 120 ttataggact gttgaaaaga ggcatcagtt ttaaagtgct tatctgccct ttgttttaga 180 agcagaccac tagagatctt ctggtgcatt cccaagctag gtaccacatg cacttgwtbc 240 ttgatgaaat gaattagagg attggggtgg tagtctcagt aacacatgag aattgttaca 300 ttctttggta ggcattgact ctdmcaggtt tgaaatgtca aatggaccct agtttctaca 360 gggcaagctc tagtcattga tgcagggtgc atgtagggac gagataaggg ctatggattt 420 ccattttatg aagtacgttt gatagaccct gtgatgctta gtagacaaag gagtaggcca 480 aatgagagta ggggaggkkc agaaaatagd gccagaggta aatty 525 56 457 DNA Murine misc_feature (1)...(457) n = A,T,C or G 56 cgcggattct ttatcactga taagttggtg gacatattat gtttatcagt gataaagtgt 60 caagcatgac aaangttgca gccgaataca gtgatccgtg cbgccctgga cctgttgaac 120 gaggtcggvg tagacggtct gacgacacgc aaactggvdg aacggntngg bggttcagcn 180 gccggvgctt tacngdhvct tcaggaacaa gcgggcgckg ctcgacgcac tggccgaagc 240 catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt 300 tctganncgg gaatgcccgc wgcttcaggc aggngctgct cgcctascsc cagcacactg 360 gcggnnntcg agcatgcatc tagagggccc aattcgccct atagtgagtc gtattacaat 420 tcactggccg tcgttttaca acgtcgtgac tgggaaa 457 57 506 DNA Murine misc_feature (1)...(506) n = A,T,C or G 57 gaattcccga aaactcctcc tgcccaaagc tcccnntagc tactacactg aatccacaca 60 ggcttggtag aaaccacagc ggtcgcccca aatctgccac agttaacgct atatgtaaaa 120 cttgaaacag actctyaaaa cccctggtag actthtagct tcttgaggga tcanttggtt 180 acagagtcag tcaacatagc aacntatdcc tccnrggcat cnnggtacgt caccaacata 240 nngsyttgnh hagcccgagc cacacaacbs ntcagbttac nncgctmgca gtachsvcnn 300 nardamgtgg stgttynnwk ggcrgcmctt nntyawcmar cnkragcyrt vkgnnnnnag 360 swkybntnsr kawyyrkgsa gccccaggac aacaagccag cagtttctac ttctgcagct 420 ctttgttctt aacagtctag ctgacaagcc accgttcact cccaaatcca ctcaccctat 480 tcaatagscc tagargtata tttaag 506 58 304 DNA Murine misc_feature (1)...(304) n = A,T,C or G 58 ggaattcgtt ggcaccaggg cgccactaaa ttaaattgag tcagcgccta aatggttctk 60 gcctgggtat caggcgtagg ttkgccagga ttyygcttcc ctaaatacgt ttttctgact 120 tagactcatt tgtaattatt gttcatttca tttgtgtttt tttttcttcc tcttttctct 180 ctctcdcdhh hnhcbtcctg tcacaatgat aacaatttag cattccagck caaaaagagt 240 ycttntttga gaagcaaaak caaggacaaa gacaagtcty cattggtcca tccagctctc 300 tcaa 304 59 471 DNA Murine 59 gaattccgct gtcttcagaa gagggcatta gatccctgtt acagatggtt gtgagccacc 60 atgtggttcc tgggaattga actcagaacc tctggaagag cagccagtgc tcttaaccgc 120 tgagccatct ctccaatccg cagttattct cttttacaaa tatttyattt ttacatgtgt 180 ttgtatgtgc ttgtatgtgc atatgtattt gtagatatcc accggagctg aaattacata 240 caggtagctg tgagcmccat gtgagtgctg gggaatcaaa ctcacttgcc tttttcaaaa 300 tmagtccacg ctcctaactg ttgagccatc tcctcaggcc ccaactttct gatattttca 360 aaataaaagt caacggtaca tctatgggca ggatcgagct atatgmaggt cmcagtactt 420 ccagggytca cgadvtagct aatgtatrct cggtgcttgc taagaactat a 471 60 32 DNA Murine 60 gaattcctct gcatagcaag tgctaggasy at 32 61 333 DNA Murine 61 gaattcccaa attttggtta aaaataaaaa attattctcc ggctctacct cgcctcccca 60 aaagataccg agagccacat gtgtgggttt taccagtacc cacgggagga atcgggtcca 120 tgtccaccca agccaaggtt aaaagcccac tcatctacgg atgagaaaat caatttgaat 180 cacctcagtt aagcgttgcc ttaatttaac ttaattaata agggggggag aragattgga 240 ggacvatact aattgaaarg ggcaagccct thacwgccyc ccaacccaaa atwaaaagrg 300 ccggyygaac mgsctttcyt ccctkgwtyy aaa 333 62 365 DNA Murine 62 gaattccccg gctcdagcgg ccgctttttt tttttttttt tagttttgtg tcgtttaatt 60 aaaaaaactc aacagggata aaaaaacaag cattttacat aatgcataca ttctcaacat 120 ctgcagatga gataaataaa agaaggctaa agcagacata ctgtgtattg cttctctttg 180 gtaagttacc aatatcctct gcagaaataa aatatgttaa aaacaaaacc catggtmtta 240 aaataattgt cccttagtat taacchaaat attcagcaat aattacagta gatgtagttt 300 tcaaattggc aagaatgcat aatactttat tctctgaggg gtaagtagct gctttccaaa 360 attaa 365 63 331 DNA Murine misc_feature (1)...(331) n = A,T,C or G 63 gaattctacc tggccacctc agacaaggag aggaadgaag atwggtccga gagctcatgc 60 aagtcgtcct ggctagaaag cccaaaatgt gcagcttcct ggagtggagg gacctcaaag 120 ttgtctataa gaagatacgc cartctctat ttctgctgcg ccatcgaagg gccaagacaa 180 cgagctgatc acactggarg ctgatccacc gatacgtaga gctcttggac aagtacttcg 240 gmarcgtatg tgagttggaa cawcatctty maactttkag gaaagcctam ctttawtctg 300 grmsgagdtt tytkawtggg tnrgggaatg a 331 64 554 DNA Murine 64 ggaattcctc gctgcggctg cgggatggtt ggcggtggcg ggaagcgccg gacggccggg 60 gcgggaccgc agttgtgaca aagacttttc atggtgcagg cttggttgtt ccagtagata 120 aaaatgatgt tggttaccga gagctccctg aaacagatgc tgaccttaag agaatctgca 180 aggcagttgt cgacgctgca agssaccgag gagagactga aagcattcgc tcccattcag 240 gagatgatga cttttgtgca gtttgctaat gatgagtgtg attatggcat ggggctggaa 300 ttaggaatgg acctcttttg cyatggctct cattattttc acaaagttgc tggtcagctt 360 ttacctcttg cgtataatct attgaagagg gatctgtttg caaaaattat tgaagatcat 420 ctggcaagca gaagtgaaga gaacatagac cagcttgcag gatgaacaag ctgccctgtt 480 agtgcagtgb ctttgaagtg ggaccagcag acggggcttt gtttttaagg aatggagaaa 540 taaatgaatt ccmc 554 65 333 DNA Murine 65 gaattccctg gaggagctca tcgactacac cggcggcctc aagcacgaga tcctgcagag 60 ccacggtcaa gatgctgaat tatcagggac actttcactt gttyctgaca cagtgctgca 120 aaagaataaa ggacactgtc cagaagttgg cctctgacca caaagacatc catagcagtg 180 ttctcgagtt ggaaaagcca ttgatargaa ttttgattct gacattaggc argtkgtggg 240 gaatwgatgg yytgctkgcc aggccagrac agccmaacgg cttctcaatk gaggtcatkg 300 gktggraaca ackttctttc cggaccaagg raa 333 66 439 DNA Murine misc_feature (1)...(439) n = A,T,C or G 66 gaattcgttc gtgcatagcc tccacactag ggttacagat tactgtgtgt gggtgtgtgt 60 gcgtgtgtgt atgtatgaga tatatactgc tagctcccca gaactagtct gtggggatca 120 tcttcctggt taactgatgc acggcccaag ttcggcaaca gcatctcaag gcaggtggtc 180 ccgggctgta taagaatcta gccaagcatg agacaattgt tttcctagct gatgcattgt 240 atttacaaat tagaacatgt caagacagca agtcttctcc ttagataatt ttcttggtat 300 ttcaaatacc tacagtgcnc tgacttcaac sctggggrrd arggarardr vcacaaccct 360 aaatacytgt ggcggctaas cgaacagaar ggggcatgtg gtgaagacca rcctgggcta 420 tatggtgaga attccacca 439 67 537 DNA Murine misc_feature (1)...(537) n = A,T,C or G 67 gaattcccgc atcatggttt gtctaatcct taggaagcga cctcgttggt tttcctttag 60 gtccaggtag tatttcctat tgtccctctc tatatagtcc gttttgagga cactgtgagg 120 atgctcttct gaccccactg acaccggtgg ggagggtgca gaatgcttct gcygcctcct 180 ggagacttgc tctttgctct ggccatgctc ctgtctgtgg cctttcaggc ccagatgggc 240 atagtgctcg atgaagtygc ctagacagtc cttcagctct gctgctaccg acagggagag 300 ggtcagttta ctctttctga tattgtcctg ccggcctctc cctatccaga cttyggctat 360 ctttaggaag cnnbcccggg agctctgctt cacgtctagg taaaaccyct ttttytsgat 420 gtccacacgt ttggaggcta gctcctggat ttcsgatgtg cccccagact gattaggggt 480 bgctgahtcg gagtagtkgg gggtagtgag aatdctgggb ctggggatag aggctac 537 68 435 DNA Murine 68 gaattccctg gttatgtggg gataaaaatc ccaggcagcc tctacccaga tgccagtcac 60 ctagtaaaaa caacccttta tagtttttta aacttaaaaa gacaacgctt gaactcagaa 120 atgtaatttc taactcaaca ctaacctggt taatatttaa taactgcagg aacaagtggg 180 gagggggcac gatgacagaa tcgattagga atttttaact gttgaatgca cataagaagc 240 catcagccaa atgaccaaca aagcagtctt aaaaattcat caggcctgag taatcgaact 300 tcagtaactt aaacccacca tggggcagtg tgcatggaaa tccctcttkg cbcctcccta 360 aggagagcag tctaaagaac agataccact tcctgckaat tccaccacac tggckggccg 420 ctcgwgcatg catct 435 69 317 DNA Murine 69 gaattccaga ctgacccggg cagccaaggt gttggagcag ctcacaggcc agaccccggt 60 gttctccaaa gctagataca ctgtcaggtc ctttggcatc cggagaaatg agaagattgc 120 tgttcactgc acagtccgcg gagccaaggc agaggaaatt ctggagaaag gcctgaaggt 180 gcgggagtat gagttgcgga aaaataactt ctcggatact ggaaactttg gttttggaat 240 tcaagaacac attgacctgg gcatcaaata csacccaasc atkgggatct acsgcctksg 300 amttctatct cctbctc 317 70 340 DNA Murine 70 gaattcggcc gagcgccgct tttttttttt tttttttttt gaggcgggca gctaaggaag 60 gttggttcct ctgccggtcc ctcgaaagcg tagggcttgg gggttggtct ggtccactgg 120 gatgatgtga tgctacagtg gggactcttc tgaagctgtt ggatgaatat agattgtagt 180 gtgtggttct cttttgaaat tttttttcag gtgacttaat tgtatcttaa ataacctacc 240 tatagggaac maagggaagg tggctttwat tkacccctgr aagggadttt tyttctgggt 300 grataggctt tttwttwttt ttccaagtta agaggrtact 340 71 398 DNA Murine misc_feature (1)...(398) n = A,T,C or G 71 cgcgatagaa gacagacnng btagagaggy ggagyaayyc agcagcagaa tncttgccga 60 gcacgaagcc ccagcttcca tccctcctgt tgcaagaaat aaattaattt taaagtgcca 120 tttaaaataa aggcattgag ccaggtggtg gtggagcaca cctttaatct cagcacatag 180 gagtcagagg caggtggatc tctagagttt gaggccagcc tggtctatat aaagtgagtt 240 caggacagcc agggtttgtt acamaagaga aaaaaagatg ttgtaatttg gagtaaaaca 300 aacacaaacc gaagaatctg ttacaggaat aatktgagag agtcacygct ttagratgaa 360 tactgtgggg ttttctcygt gtgttcttgg ggtgtttt 398 72 618 DNA Murine 72 gaattccccc taactgcttc ctgctagaac atcaatttac tttatcaagt tcatactcgt 60 gctttgaaaa gaagaacagc aacacaccac agcatccatc gggcctgacc ttctcaaagt 120 aaacacagag gggcctctga aaggcaagaa ccattaactc ttaaaattct tcctgccttg 180 gagtggaggg ggtggggagg cagtggatac gtgtgcaggc atagtagtga cagaactcag 240 ctgatgttct ggggttgggc ctgggagaga tatcatacag gactcggccc atttttactc 300 tctggcctaa agattttgaa ataggaccaa gttgtccatg aagaggggct gagaagccag 360 aaactggtat tatagcataa ttttagaact ccgtgtgctg tgatgagatg ctgccaggct 420 gagctgcbgc ctctgagatg ctcggcagtc agagtgttgc taagaaaacc cctcagtata 480 ggaacagact ctaggtgcct gacatttgtg gctctagcat ctatattcaa tagttthcac 540 atgataggcc tgtaaaacat atgtttctga ggacaagaca tttctaagag agctctggag 600 gttatttgaa caggtttt 618 73 531 DNA Murine misc_feature (1)...(531) n = A,T,C or G 73 gnggcgcagt gtggtvgmat tcttatacaa accgacaact gtcaccaaag cttataaaac 60 acgatagtac tgtccctctt ttctgaacca tcagaagaca caaaactgtt agtgacacaa 120 acggtgacag gtagctggga cctaggctat cttattatga aggttgtttt gcttgttgta 180 tatttgtgta tgtagtgtaa cgaatttgta ccatagagga ctgtccgtaa ctactgttta 240 gcttctacac attgaaatgt agatgtttca ttggctgtct gaaaaggtgt ggcttgtcct 300 tcctagagag atctacttaa aaactgcttt gtgacaaaaa ccacacctga agaaatttta 360 agaatttggc ccagttagtc actctgtgta atcccggaat ctagctgctg aagtcttgcg 420 aagtaaactc cccgtgaccg atgtcagtta agctggtgat acctggagad gtggtcagtt 480 gctaaggaag tggatttccc agtaggggtt tctgcacctc acctgtatag g 531 74 491 DNA Murine 74 gattcgaaca taccacctct gccccatava ctgttctctc cgggggaaaa aaatggaagt 60 tacctcacag ttcactgccg tggtatttca tctgtcccat gctttgcatg attgccatgg 120 tacagcattg tttcaaactg ttcactgtga tctgtgggtc tttgagtttc agtgagtttg 180 ctgaaatgtc gaagaaatat ttccaaactt caatgttcaa tgaaattttt gttcaagttt 240 gaaatggaga gagcagcttt aaaaggtact aagcctttta caaattggtg agtactggca 300 catgagacct agagcaggac caacttctca cacatagtca gtgggaaaag aaagtgcctt 360 gaaagttcct ccctcmccta cacagtagtc gtcatgtcga gacctgccag agagagacac 420 attctcaagt gaatcctggc ttcttggaag cgccttscct agacgagaca cagtghcatt 480 aaaacaactt t 491 75 389 DNA Murine misc_feature (1)...(389) n = A,T,C or G 75 ggattctcta cataatttga aaggaggcan ngtctcacta tatggctaag gctatcctgg 60 aacttgcgat cctcctatct cagccttcca agtgctagga ctacaggtgt gtgcatctcc 120 actatcaggc ctcacttgta gatgggaaac aggagtgccc catctgagaa tatgcatggc 180 ctcactaata aagccaggac cacaccacag cagtccaggt tgtctbcggc gatgggctga 240 ccttctggga catatctact ctatgtccaa gccaaggaca ctgmctttcc ccatgtgaac 300 ctagtcctca gaaatgagcc aycccttcga atggatttat gccactggat gtgaaaaggg 360 atgctgttgt tttgttattg ggaagccct 389 76 605 DNA Murine misc_feature (1)...(605) n = A,T,C or G 76 gaattcgctt gcttcaaagc cagccttttg gatttcagat gagccgcggg tacccgcaat 60 ctatgtgcca ggacgccaga cccgcttatt gaaatcagag ctctattttg ccggctggga 120 cccaccgccc agagccacct aggtgctagt cgagggcgca cggagctgag ctctcccgcg 180 gctcctgcac ttccttcggt ccggcctggt cttggcactc gggctgcttg atttggtggt 240 gcaagaaagg tatgcgttgc atacgcccta gccctttgct ccaacgctct cagccccctt 300 ggctcagaca gtccactcct aggtctggtt ctcacggcct tccctgcagc tggcttagct 360 gagaaggcgg tgagagtcgc gtcagcagtt ttggaggaga aagtgcgggt tgattattga 420 cccacgcctt ctttcttcaa atgccacatc cgaccctgag ggtttgaaga gaaaaagcgg 480 ccgagcbghw ttnnycggcc ggctctcacc tcctamacgt cccgggctct tccctttcaa 540 gttgcgccgc tgcaatctgc cataaggagc aagtgtttgc tgttttgtgc tctgtttaca 600 gcttt 605 77 465 DNA Murine misc_feature (1)...(465) n = A,T,C or G 77 gaattctaac gcgtgcgcga gtcaggggct cgtccgaaag ccgccgtggc gcaatgaagg 60 tgaagggccc cgcccggggg gcccgaggtg ggatcccgag gcctctccag tccgccgagg 120 gcgcaccacc ggcccgtctc gcccgccgcg ccggggaggt ggagcacgag cgtacscgtt 180 taggacccga aagatggtga actatgcctg ggcagggcga agcagaggaa actctggtgg 240 aggtccgtag cggtcctgac gtgcaaatcg gtcgtccgac ctgggtatag gggcgaaaga 300 ctaatcgaac catctagtag ctggttccct hcnaagtttc cctcaggata gctggcgctc 360 tcgctcccga cgtacgcagt tttatccggt aaagcgaatg attagaggtc ttgggggccg 420 aaacgatctc aacctattct caaactttaa atgggtaaga agccc 465 78 681 DNA Murine 78 gaattcgcag cagcagaaga tgggcgtcta aaaaggggcg atcagatcat tgctgtcaat 60 gggcaaagtc tagaaggagt gacccatgaa gaagctgttg ccatcctcaa gaggacaaag 120 ggcaccgtca ccctcatggt tctctcttga agtgactgcc agagctgaag cagcccagcc 180 actggctccc ctcctactgt aacagagagg acctgtttgt atgctgtgtt ggtcggagaa 240 aactacaggg aggcgagaaa cagagtgttt gttactcaca gccaagcatc atttttcctt 300 tactctgcat ttcatgatca tatactcaaa aagaagagat atttgcatag ataaacctca 360 gttttatctc gacaatatct aacaatttaa ggtcacgtgg acaaaattat tatatgttca 420 tcttgttagt gtggaaacaa aatgatacaa agttaggcaa ttaggttaaa gatggaaatt 480 tagagaaaaa gaagacagtt ttgagtttta taggacttct tcaatccagc agtccaaaag 540 aagaaaagaa agtgcttgca atacttttga atagtctact gttttaaaat tgtgacatat 600 tggtcctact tacctctaat gcatattttt ctgctaaaat tgtttagcag tccttgtaag 660 ctttaaaagr aattccygtt t 681 79 538 DNA Murine misc_feature (1)...(538) n = A,T,C or G 79 gaattccctt cagaattgtc accccacata aaaagttttc catcctcagt aagagcagcg 60 gatgtattgg cgccagcaga gagctgttta atggtatcag caggtgtaaa gaagacaatt 120 tgatgaaagg tgtctctatc gtcagtgtca ccaagcccca gttgaccttc attatttcca 180 ccagctgcat atacgccacc agtatctgtt gaaactaagg tgtggttcct tccacaggca 240 gcaagtttca ccttctcagg cttaagagct ttgatacatg ttggcttgat gatagcagct 300 tttgatccta atcctaactg accccagttg ttactgccga acatgtacaa tttattattt 360 cctgtaacaa tagcagtatg ttcatctcca catgaaagac atatgggtat gtcattttta 420 aaccagaatt tgctaggaat attttcggca aatttagttn nncaaacgtt aaaaacagca 480 cctgtatcgg gcaccagtga ctcagattcc gccatgccga agcctgcgaa cggaatct 538 80 130 DNA Murine misc_feature (1)...(130) n = A,T,C or G 80 gcgcttctng ckrnngtcat ggcatcntag gagngtgscc aatbrcgcsc ctattakgtn 60 gastgcgthn tttarcratt tacasctkgg gccggttcgt tttttagcva accgtayggt 120 sgatcttggg 130 81 422 DNA Murine 81 attctcaggc ctccttagtc actgagacca ggctcttccc atcaaactcc ttgagctgct 60 gcacgcagta ctcgtcaata ggctcagtca tatacaccac ctcgaagccc cgcttccgca 120 ctcgctccac aaaggcagag ttggccactt gctctttgct ctcaccagtg atatagtaga 180 tggacttctg ggtctccttc atgcgagaca catactctga caaggaggtc atctcatctc 240 cagactgaga ggtgtgatag cgaaggagct cagagaggcg gcbgcggtta gtggaatctt 300 catgaattcc aagctttaaa ttcttggaga aggcctcata gaacttcttg tagttctcct 360 tgtcctcagc cagctcggag wakatgctyc ahggcacttc ttgacgatgt tcttgcggat 420 ga 422 82 383 DNA Murine misc_feature (1)...(383) n = A,T,C or G 82 cgcagtgtgt sntcgcattt agtttttttt tybbgcacct tattcctgtg gtgtcttcac 60 tagagataat cagggtgcca ctactgcttc ttactttgat acctttagca aaaatcccaa 120 tgaggtaatt tatggtttag taaatgaact caatagcttt ttkgtttcaa gagtccaaca 180 atcctaattc cttgaacttt ttcttagagg ttatattttc caatcttggt tttgtttctt 240 ttaawtttgt tcyttawctt tctctcattc tyacgkkatt tctgaaacaa caccccacta 300 ggaawttgag cccmcagttc aattkgacct cacctcctaa gaagtgggsc ttcttttcag 360 tggaccacca ctwaaaggra aac 383 83 609 DNA Murine 83 gaattcctgt gggcaatgac acacacacac acagagtgag ggagagagag acagatacac 60 acatacattt gaatgaaatt ttaatttaac tcatgtaatg cccttgagac atggaaaacg 120 cagttgtgag gttaaaccat acaagcttaa gactttgaca gcatcaaatt gatcaccacg 180 tttactgtca gaagcacaga attcatggtt tcccactttc tttcctacgt tagataagct 240 tgctagtgta gagtttgtca taggcgatgt cttgttcaga taggctgtta acgattcaca 300 gttgtttcta attaaatatg agtttttaag ttattgatgc ccccatgtgg tgaaaagcgt 360 atctttcctc tgttagaact tggaaatgac tatattttca ttttaataaa agtggataat 420 aatgtttttt ggaaatgctg ttgatcaggg acataatttg aattttgtaa agctcattgc 480 cataaaattc acagcctcac cctgtgttgt ctcagaagtg catgtaacca agcacgccca 540 ttgagacaaa gtataagaga gactgagtta tagaatagcm tagggcttth tcygatccat 600 gtttgdtga 609 84 325 DNA Murine 84 tcagaccaac atcaatcgat tcattaaata tcttacacta ttcctgatta ccatgcttat 60 yctcacctca gccaacaaca tatttcaact tttcattggc tgagaagggg tgggaattat 120 atctttccta ctaattggat gatggtacgg acgaacagac gcaaatactg cagccctaca 180 agcaatcctc tataaccgca tcggagacat cggattcatt tagctataag tttgattttc 240 cctaaacata aactyatgga gaacttcaac agattatatt ctccaacaac aacgacaatc 300 taattccact tatagggcct attaa 325 85 360 DNA Murine 85 ttcgatggat tccatcgagg cttgcctttg ttgccttgct cacctgttga ttgctataga 60 gtccctgggg tccaggaacc tgcaagagat gggggtgaag gcctcctatg cataggttcc 120 atatcagtgt gttgcttgcc tggtggcagc ccacatttgt acccacttcc tctgctgctc 180 taggagcctg gaacatgctc ttccccagcc tgcctctggc tttccctgtg gtcctactcc 240 gtgccacagc acttgggaag tcttgtgtac taagtctcct gatagccagt gcgctgcttt 300 agargtgtgg ccgccttccc accggcgtgg ccggggacca tccatttctt cttccttctt 360 86 456 DNA Murine 86 gaattcgttt cctgacatca agaaaacact gcaagttccc aggacaacgg ggacagagct 60 gaagctgggg acagaagcag ggtgctccct aggctacttc tgtctggttt tccagccacc 120 cagaccctga cttggggcgt gagtccttaa aatagctaca gtacaagtag gtatatgaaa 180 gtggagtgtc cttcagagtt caagctacta caaaatgata cctgtcccct ccagggaatc 240 ccaattcaga agtcagaatt aaagtggcca attatctctg agacagggag agagagacag 300 ccttggaacg ttgcatccat gaggacagta atttgtaaat gctaaatggt atcccccttc 360 atacaatgtg gcaaggsata tatgtcttaa aaccagcttg agccaggtat ggtgatacac 420 yyctgcaatc caaacamytt gggaggcgta gagaga 456 87 274 DNA Murine 87 ggaattcgat cggcctatcc cactaaactg ctggctggag ctctgagagc tcctccctgc 60 tgaggcggtg ctgctcgccc cgtaagtgcc agcagcatac tcctgcgccg tgtagccact 120 ggttgccata ggcagctgcc ccataggtgc cttgagcata ggtgtattgg cctgcttgtg 180 ccccaaaggc agaatttggg cttccatagc cactgccatt agcataactg gctctatcgg 240 gtttccacta csgatccctg taagcttgta gaat 274 88 521 DNA Murine 88 gaattcgtaa aaggaggcct cgaatctgag tgacaatggg cccttctact ccagggacaa 60 tgattgtatc cccttccttc aaacgtccat tgatcaatat gacatctatt gtggtgccca 120 ttcctgggag agctttaacc tccatgactt gtgctctcag ctcttcacag tgtgcaagcc 180 tcttgctcaa catggtttga gttaactcca caagaaggta gatgagactt cccatgccat 240 caccagtatg tgcagaggta ggtaccaagg acacgaaagt gcgggggatc tttattctca 300 taaaacaaag cagcattcaa accctgctgt gcaaattcta caataatggc ctttgcacgc 360 tcctcaaatt catcctttgt atccttcttc tgctttttta aagtaacagc cwcatctagr 420 atcaggastb tttyttccaa tcatataacc tgttcaatct ttattaagtg caacaatgaa 480 ggggcacttt ttagatttga gaatkttgat tgattcaatt g 521 89 575 DNA Murine misc_feature (1)...(575) n = A,T,C or G 89 ctcagctatg cadvvvnntg gtacgagctc ggatccacta gtaacggccg ccagtgtggt 60 ggaattcttt tttttttttt ttttttgaga cagggtttct ctgtatagtc ctggctgtcc 120 tggaactcac tctgggatca gggtggcctt gaactcagaa atctgcctac ccctgcctcc 180 caagtgctgg gattaaaggc gtgcaccacc actaccgccc ggccactgat atgccttaag 240 tgacagacat tatgcttgtc aattagcttt cacaaacagt actgtctcta caaggcattc 300 agatacaagg agcctcaagt atctcctacc tgataagtca tgtcaagagg ctgcacttca 360 tatggggtca tttataatgt acatgatttt atttgtatat tactactgat catgtaccag 420 ggaaactatt ctcagaaccc agtttttgtt ggaawacaaa aagtgcaata tatgactcaa 480 gtgcaaaara aatcctccaa ttttatttct gtaaggacag gctgggcctg atgcacacag 540 gtccctcccc ggactagtaa ggcaaratgc agcta 575 90 449 DNA Murine 90 ggaattcttt tttttttttt tttttttttt tttttagaac aactcagcaa aataaaattc 60 cggtttattg ttggacattg tttcacacat acatcaaaca ggccaaaaaa aaataaacag 120 caacttcata gacagaaaga aaaggaaaaa aaaaatcttt ttatctttgg cctttttaac 180 catctcatac aaaccaacta cttatagtac agctaggtac atacacaaaa gttactggaa 240 tgctcggaat aagattgttt ttttgttgtt gtttttgctt ttttttacaa ggtttttttt 300 ttctcctttg agattataat gaacatggtc acaccacaag taaagtctga agtaggacag 360 aaaackctct gaaggctggt ttggtcaccc gttatcatta aaaatggctg gacccttaac 420 aatatgttac aaaaatttaa aatgttaat 449 91 487 DNA Murine 91 ggaattcttt tatcataaaa gtgttgacgt ttatttatta tagcaccatt gagacatttt 60 gaagttggaa ttggtaaaaa aataaaacaa aagcatttga cctgtattgg gtggttgaaa 120 cagcaaaaaa ttgtattctt tttttgtcaa attatgcttt ttccaaaagt ttggaaataa 180 ataactggaa tttagttggt cacttgcact ggttgataag attaaaacaa gatgaacaca 240 tggatgtggt ttttgttttg ctggggtttc agagagtttd gcttataaaa agcaaacagg 300 kccaatgtcc acaccaaatt cttgatcagg acccccaatg tcatagggtg cgatatctat 360 gatgggtagt ctcattdcct tgcgtgtttg atattcaaag actgtcttdc dccattcccc 420 agtgtgttta gtacagccat tcctctagaa ctgtgtaagt gaatttdctg tttccttcca 480 gccttga 487 92 399 DNA Murine misc_feature (1)...(399) n = A,T,C or G 92 ggaattccag atcagctcca acccggngct ggcagccatc tttgaaagta tccagaaaga 60 ttcttcgtcc accaacttgg aatcaatgga cacgagttag atgtgtgcnc cccgtgagga 120 ccattccatg tgaccgcaca atgcactgaa cgacaggttg accacagcca cgggagagaa 180 gtgtccagag cttcacgatg ttccacttta ctttccttcc cgggaagttt gtttggcttt 240 cttccattgt tgtttttgta gcttttwctt cagaagtctg tatttccata agccagaggt 300 tgtaaagcca ctgatgtttt tagtggttag ggcaacattt gaaatgggaa cttaaddnct 360 tggatttatg aaatgtggaa atagggtcca gtatctgtt 399 93 343 DNA Murine 93 gaattcccgg gatttcatga tttaaaagga aacatggtgg tattaaccca cttggcaggt 60 gtcaaatcct catgaccagc ttaagacaga tcctagacgg aaagggaggt gcagcccaag 120 tcagggcttt ggggtgcaca gggagccagt aaggaggaga ccgtctgggt ttcttcccag 180 atgttaacat cttcttggct cttactcact cccacccttc ctcgtaaaca aatcaaggcg 240 agccctctaa ggctggagat agcccagtcc agctcagatt taatactcta gcccttcccc 300 ttgtgttatt ttthmcmagc tgccttctgc ctccaacata tga 343 94 203 DNA Murine 94 gaattcgaac aggccaatsa ggagcttcga gaacttaycc agaatgtsaa agacttscct 60 cagccgtgag cctcccatgt ggcccaggcc atgtgcttgc ttcccttgtg tctgtgtgta 120 cttgagtctc ggtgtctgca atggacatgt gtttatracc ctatgtctgg ccctgagtcs 180 ctgtccagtc aatgtsccta agt 203 95 441 DNA Murine 95 gaattccctc ctcccgcagt tgacaagcca agccgccagc tagcttcatc accaactcgc 60 tctcgctcca ccatcctgga accctttccc agcttcacca ccacatccgt atggctcctt 120 cttcctagct tcctccaccg aaccgcactc tttcctgggc tatcttcacc atgcactgct 180 gctgchggct cctcagtcct tcctagcttc accaaactgg cttcgggact cctgtctgcc 240 gctcctgtct tcctagttca ctgaatgcac ttctgtgtag acctgggtca gctgccaatg 300 ctagtcgtta ggattttaaa agcacctcag ctcaagtcca atgcaaaatg ctgacaatct 360 tgaaactgtt atcaaaagtc cttttgtcat caagcaaaat taagctacaa gttaaggctt 420 ttaatattct ctaactctta a 441 96 390 DNA Murine misc_feature (1)...(390) n = A,T,C or G 96 gaattctgga agtgtgagcg tctctggagc agattttttc cggggccggt ctttgggaat 60 ggacagaaat tctggcgcat ctgtggagag aggggtggat ggggcgctgg agggggcgct 120 gcgcaccgag gaaggcagta gggcgatgct ggagatagaa atggccggtg ggaaawhgcc 180 aatcttcttg ttggtggctt cctgagtggc tctttcgaac tctcgcactt catccattgt 240 catgtcttca aagggaaaag cggagaaaag aatagttact gttcggacbg gcaaatgggt 300 twhnhhnnct aaatctgggg acactaccat gaagctgatg cctacccaat cacaaacttg 360 acatgtcttt gaaatattag accctcattt 390 97 426 DNA Murine 97 ggaattcctc ggtcatcact gggaagagag gcccctttgt cttaaaattt ttatatgccc 60 cagtacaggg gaaggacagg gccaagaagt gggagcagca tggggggggg tgattttcgg 120 gatagcattt gaaatgtaaa tgaaaaaata tctaataaat tttttaaaaa gccagatgtt 180 aaaatgtgac aataaataaa taaacaaaca aacaaataaa tgttttacaa cctaaaaatt 240 ttaaagaaaa aatgaaaagt ggagatgagg gccccaattt acctaatttt actgctgcat 300 cctattggaa aataagtaac aaaaactgtg aaattgttgc atgttttctt ggtatttgtt 360 ttaatgaata gtttctaaac dcagaaatcc ttgtggaggc agcgcagagt aatgcattga 420 tcatca 426 98 385 DNA Murine misc_feature (1)...(385) n = A,T,C or G 98 tctgagacaa ggtcttagtg tacacggcct gcatgacctg gcctcctgct taaagaaatc 60 ctcttacctc tgcctcccaa acgctgggat tacaggaaca tgccaccaga tacagccaaa 120 atcattacct tttctttctt cttttcagta ccagggtcct acacatgcta ggcaaactct 180 ccaatactag ctacacccac agctcagcga cacaagctcg tctcttgtgc ttgagtctac 240 agtgaaagtt gactcaactg aaatgtttac cttgttgatg ctgtaacact gtctgagtcc 300 agaaggtttt cagtcatcct taactgcagc acctctggca tnyngtctga cttttctaca 360 ccttcttctg gaagttcttc tatat 385 99 299 DNA Murine 99 ggcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc 60 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca 120 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc 180 ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac scgacctcgt tcaacaggtc 240 cagggccgca cggatcactg tattcggctg caacttttgt caatgccttg acactttta 299 100 390 DNA Murine 100 gaattctttt tttttgttat tatctgaaat gatgttttga aacttctttt gtctctgcct 60 cacccccaac ctactcccct ctccaaatca caaactaggg aatctggaaa ccaaggaaaa 120 taccaaatcc agatttcttt tgaagaccta gaacctttta agatgactcc tttcagtgct 180 attggtttgg agctctggtc catgacatcc gacatctttt tttgacaact ttatcattak 240 tggtgaccga agagtagttg atgattgggc caatgatggg tgggggcctg aagaaagctg 300 ctgatggggc tgctgaggtt aktgattgtt cattaattgt ggatttwtat ccactttttg 360 gggggagact gattactttt taaaaagcag 390 101 389 DNA Murine misc_feature (1)...(389) n = A,T,C or G 101 ggaattcgtc agtgagtgtt gactcatcca aataccaagt gctctggtct gaagctgagg 60 gccctgctgt agggtccgga gccccacaca ctgtgttgat ggctgtggac tgggaggaaa 120 ggagctcgtc tagaagacgc tgggctgtgg ggagaatctg ctgaggaagc tcactgataa 180 ggtactgagc aaatttttga agctggtccc tttgtagccg agacagggac tctgagactg 240 gagcccgcag gcagactgca gatgcgttgt gaatgcggaa gaggcagagt gccacgacat 300 gggtgcacca tttggccccg gccccacagg tacagctaca agaagtgacc cggcagcngt 360 caaacatcac agctacattg taggccccc 389 102 344 DNA Murine 102 ggaattccag atatctggcc agcatcctta gtggcctgtc gctgtgaatc attgaaataa 60 gcagggactg tgatcacagc attttttgct gtgtggccca agtaattttc tgcagtctct 120 ttcatcttca tcaacacaaa tgctccaatc tgacttggag aatagagttt tccatgagcc 180 tcaacccaag catcaccatt ggagcgcacg gcacaatttt aaaaggacac atctcttagt 240 gtcttctctg tcactctcag gggtcactca tactcgctcg ctccaataag cacgcttagt 300 acgcatagaa ggtattgttt ggattggtsa cagcttcccg tttt 344 103 354 DNA Murine misc_feature (1)...(354) n = A,T,C or G 103 ggaattctat ttgtaacccc ctaatttgta accctgtaac ccagggaggt tagacaacac 60 tcattccctg gtgtcttttg tctcactgat cagtcagaac ccagcctgaa agcagttgta 120 ggactgtttt ctaagccctg ggcagcagag gcaggattag gagttcaaag caagtcttaa 180 ctacatggca taaagaaagt aggagctaca ggagatgttt ctctaaacag acagatatga 240 aatctcttta aaaacaggga atgaaattct taattttggg gagcaatatt ggagaactgw 300 tncacttaag agatcaccca tgtgatagtg aaaaatgaaa tttaaaatct caat 354 104 387 DNA Murine 104 ggaattcggc tgaggctgca atgtgaggtt agatgtggag tcacgctgtt caggtttctc 60 attaagagga ttggcagtga aattgccttc caaagaactc tgcagtggga tgtggcacaa 120 ttctgagagt tgactctgat gcattctttc aggtttttaa cagtatttga ttataaacat 180 atggatattc aattgagaca atttttattt ttctccctgg gtaggaagaa ccactaagta 240 aagggcaagc tgggcttgcc tgctctctct gtccagttct acattagtcc agtctgcaca 300 gtgtcccatg ctgcctgtaa wcacaaattg tggttcttgg gttaagagtc atgtgttttc 360 cagaccttga actctctact gagcaga 387 105 269 DNA Murine misc_feature (1)...(269) n = A,T,C or G 105 ggaattcccc ggctcgagcn ngccgctttt tttttttttt tttttttttt accatgcaac 60 aaaaccttta ttaacatttt ttaacagagg ttcagctatt attgaaactt gtaatttcta 120 aacttaaatt ggggcaagtg gctagagtgc agagtaatgc catcactgcc cactgggaat 180 gcagaccgaa taattaatag ccannncnnc agacggagag accaggtgca aggtcgactc 240 ctttcnrgaw ggttgtaatc agagagagt 269 106 464 DNA Murine 106 ggaattccca gaggggggat ctcatcagga aggcgatgag gatgcctcgc gcatggaaga 60 ggtggattaa agcctcctgg aagaagccct gccctctgta tagtatcccc gtggctcccc 120 cagcagccct gacccacctg gctctctgct catgtctaca agaatcttct atcctgtcct 180 gtgccttaag gcaggaagat cccctcccac agaatagcag ggttgggtgt tatgtattgt 240 ggtttttttg tttgttttaw tttgttctaa aattaaaagt atgcaaaata aagaagatgc 300 agttttatag aattccacca cactggcggc cgctcgagca tgcatctaga gggcccaath 360 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg 420 aaaacctkgc gttacccaac ttaawcgcct tgcagcacat cccc 464 107 328 DNA Murine 107 gaattccgga atggcatgat actgaagccc cacttccaca aggattggca gcagcgagtg 60 gacacttggt tcaaccagcc ggcgcgcaag atccgcaggc gcaaggcccg gctggcgaaa 120 gckcgtcgca tcgcccctcg ccccgcgtcc ggccccatca ggcccatcgt gaggtgccct 180 acagtgagat accacaccaa ggtccggkct ggcaggggct tcagcctgga ggagctcagg 240 gtggctggca tccacaagaa agtggctcgc accatcggca tctctgtgga cccgaggwdg 300 cgaaacaagt ccacggagtc actgcagg 328 108 526 DNA Murine misc_feature (1)...(526) n = A,T,C or G 108 ggaattccgg atctcttctg tgttcccact actcaagcac cgagtggcgt tctatggcgt 60 ccgcctcggc tcagcccgcg gccctgagcg cggagcaggc caaggtggtc ctggcggagg 120 tgattcaagc gttctcggcc ccagagaatg ccgtgcgcat ggacgaggct agagacaatg 180 cgtgcaacga tatgggcaag atgctgcaat ttgtgctgcc cgtagccaca cagatccaac 240 aagaggttat taaagcctat ggcttcagct gcgacgggga aggtgtcctt aagtttgccc 300 gcctggtcaa gtcttatgaa gcccaggatc ccgagattgc cagcctgtca ggcaagctga 360 aggccctgtt cctgccaccc atgacactgc cgccccatgg ggctkcttct tggaagcacg 420 tbtngcagcc tyctgagatt bgttctcgta tgtgtkcctg cctgctgttg gargccggcc 480 cttgtgttcc agaggrtaat aaatgtacht gtgactcaaa aaaaaa 526 109 598 DNA Murine 109 gaattctaac tatctaaaaa tatgaatgga taaccaaagt attccaaacg tggctattct 60 gatccaccgt ttgtttttct cttaaaaaaa aaaaaagtat gtacagaaat tgtataaaag 120 actttgtgaa ttcaatgaga gttagcttcc agtcttcaca tcccaaatgc tgggtttaca 180 gttttggctc ctttgcatat ttgcctgtag aattaagact cataattttt gccttgctaa 240 cagaacacac tttaaattat gaaaagccct caacatatac caaagtaaaa gacagcattt 300 tgaaattagc caaggccaac atgattctgc tctctggaac cagtgtactc tagtgaattt 360 ggtgcttgtg gtgagtgaga aacgacaatg ggaaatgtct actgtttgac ttttgaaatc 420 agatttattc agtggtggct ggacttgggg atgggttcaa tccaccattg yctggcacat 480 gttaattact aggtaaaggt caaatacaat kthagaccta aagccacagg aggaggatgc 540 aaaacgttca attccaaaga gaacagtttw gwgttcaaca acatgggact ttwcctag 598 110 474 DNA Murine misc_feature (1)...(474) n = A,T,C or G 110 gaattcggaa tggtggcgct gtgcctgtga gcttccgaag ttaatggatt gttctggctg 60 tgacgaacag gatgacggtg tcaggcgact ccagccaaaa gctttgcaaa gtggctcgag 120 tcacagtact ctgatgctga ggcaggaggg ctcccagttt gagtcagcta gggctcaaac 180 caacccaaaa aagcctgcca agtgaaaaaa gacactttcc agagctgttg caaggtgcag 240 ctggcagcac agcacagctc agcccatccc agcccagaag gagcagcgcc acccacaggc 300 gcagggagga agtaggaagg ctgcaggggg caggcagctt tccctgggac aaagaaaagg 360 aacatttggt ctctcagtgt ctgctcttct agatccaaat acacagtacn cctttgctgg 420 tgttttgttt tgaattaaag aatattaaag tttgggggaa ttcaccacac tgrc 474 111 409 DNA Murine 111 gaattcgtca ataaggtata ggctacaccc ttctcaccag ctcttcctgt ccggccaatc 60 ctgtgagtgt gcgtatcaat gtcccgtgct acatcatagt taatgactgt cttaatggaa 120 ggaatatcca gaccacgggc tgcaacatca gtggccacca ggacggggat gtcctttttc 180 ttaaaatctg aaataacctt gtttctttcg ctctgatcca tgtccccatg gagcagacca 240 agattatgac cctcctgctt caggttactg gctagctctt cagcattggc tttcttagta 300 acaaacaaga gcacactccc cgaggaagta aactccacca gacgccgagt cagccagttc 360 catttactkg gtccggaatg gagaatytcc acaatctgtg tcacatytt 409 112 331 DNA Murine misc_feature (1)...(331) n = A,T,C or G 112 113 373 DNA Murine misc_feature (1)...(373) n = A,T,C or G 113 ggaattcgtt ttggaataac tggtcaacaa aaatcaaaag atgtctgggg ggtgggggga 60 gactgcctgg cagtacaggg tgggggagaa actccataca acaagacagt gcaaatcagc 120 aggaaactgc atgtgtgcac tccagacagc caatccagga gcatgctgtg cattctggaa 180 ccctccagat gagtgcagaw wtdtggcaat gccccatgca ttcaccttta atgcaactgc 240 accagcccta ctgtgagtga tgtgatctcc ctttaaaaac cacccaccat catcactgat 300 tcaattatnn yygcaagttg tatcttcaag gacggaagcy ctgaagtgac cattcacnad 360 cttataattt ata 373 114 312 DNA Murine misc_feature (1)...(312) n = A,T,C or G 114 ggaattcgtc tacagcaacc aaagagataa caacagtagg gtctgaaatt tcaagggctc 60 tggggttcca ggccagtatc attcacagaa ggggatgggg aggagggctc cagaggctgc 120 caggctaagg ctatacagaa ggbcctccat gaaaagaagc tttatgaagt ttctccagaa 180 actcaaatyt ggagatattt ttaaaatnnc tcaggctgtc ccagcagaga atncctgtga 240 ttatkcctga gaacaaaagg rgacaggcct cctcctgtgt gggagctgta catkcyctca 300 caggtktgtc tt 312 115 279 DNA Murine misc_feature (1)...(279) n = A,T,C or G 115 ggaattccag ccctacatca agagagccgc agccaccaag cttgcttcag ctgaaaaact 60 catgtatttn nnmmctgacc agctgggact ggagcaagac tttgagcaga aacagatgcc 120 anahnggaag chgctggttg acrgtttnmt tctgggcatt gatgttagca ggggcatnna 180 hchggaacht cgatgatcag ctcaaatttg tctccaatct ctacaatnan cttgcaaaan 240 cnaaaannca tagtggtagt nctgactaag tgtgatgag 279 116 380 DNA Murine misc_feature (1)...(380) n = A,T,C or G 116 ggtgacacta tagaatactc aagctatgca tcaagcttgg taccgagctc ggatccacta 60 gtaacggccg ccagtgtggt ggaattcggg taagcacact agcaaaaaaa anaaaaaaaa 120 aaaaaaaaay ncaaacaaaa gagtcttaga ggaagaatga agaaaacata caatactttc 180 aatttgaaga cagatgcaca atactttaac atatgccaaa gattaaaggg aaaagattac 240 aaaattatat cactgcaaat tttgttgctg tgacaaatta aaagcagttc ataccagaaa 300 cacacacagg tgcagaccgg tgagcacaca ggcaccatgc attgacagtg atgttgattc 360 tttaaagtaa tgagccntgg 380 117 558 DNA Murine 117 ggaattcgtc actgagtcct ctcttcatct acattgtcta ccagccacta tgaaagcctg 60 agcccgtact tgtcaactat ccaggaggat tatcccacct tgttacctca cctctaaaag 120 cagataacag cctgctgctt gtttttgtaa ataaagtact attcaaacag ccacacatac 180 tcaatttagc tattgtcggt gattgctcac agacaagaca agttgttgag acagacaagt 240 gtggtcacaa agcctaaaag tatttactat ttggcactat agaaaaaatg agaccgctgg 300 ctttatttag agaatgagaa gccgttcgct aacagggatg atgatgatga gtgtgaggaa 360 ggaataactt ccaacmgttg tgacagctta ttttatagaa aaccgtccca gcaaatttat 420 wgtcactgtc cattcattaa cvgctggtca tgttcatgtt cccagtagca ggtcatctgt 480 caataaactc ctgataccca gagctgttyc cagtyccact chaactttag cactactgtt 540 tacctaggcc ctcaccct 558 118 364 DNA Murine 118 ggaattccaa ttcagaaaaa aaattcagac tgaaatgact aatcccatat ctcataaccc 60 cttcaaccag taacaccccc ccccaaaacc cattgtcttc agtgtgtcag ctcactaatc 120 taatgatcag atcaatctat gaactccaca acaaaatagc tactgagcag cccttcctga 180 gaagtaaata ttctagattt tgggaaccag tgccgaagac agaatgctta ctgtctagaa 240 gtttcacttt ccttatgagg gggttgagaa ccaagatgac tattaatgtg tgatgtgatc 300 cmataaaagc tgtkgggaaa tcaggttttg aggaggggaa tagttgtgca aaaaaaaaaa 360 atat 364 119 518 DNA Murine 119 ggaattcgca gatttctttt ggacagtgat gggaagagtc tcatctgtaa agtgaaccta 60 tcaaagatca atagcaaagt cctgaagagt ggtcagctgg aggatacatg tctggtagag 120 ctctcactgg ccctggacct gcgcctacag gtcagcgtca gcagttggca tctgacggct 180 gtcactgtgg atgtgtggac actccatgct gagctgcatg aaggtctctt ccatagtcag 240 ctactgtgtc atgccccagg ccggatttcc aaatcagttt cttgttcaga tttgactgag 300 aactttgctg aaccaactct gcctgggcct atacctcctc cagcggctgc cagaccaagt 360 caaggtgaag atggagaaca cmagtgtgtg tgttgtctat gaacagtcaa aaacbgcact 420 tgacttkgac actgaagctg ctgcawtttc ctgtaccacc gtgatgagga ccaactgccg 480 cttcgaagcy tcacagcaaa ctatgatatb gcacacga 518 120 518 DNA Murine misc_feature (1)...(518) n = A,T,C or G 120 ggaattccca gggtgcaatt ggtagtccag gacctgcagg tcccagagga ccagttggac 60 cacatggacc tcctggaaaa gatggaacaa gtgggcatcc aggtcctatt ggaccaccag 120 gtcctagagg aaacagaggt gaaagaggat ctgagggctc gccaggccac cctggacagc 180 caggaccccc tggaccccct ggtgcccctg gtccctgctg tggtggtggt gctgctgcca 240 ttgctggagt tggaggtgaa aagtctggtg gcttttcacc ctattatgga gacgatccaa 300 tggatttcaa gatcaacact gaagagatta tgtcttcact caagtctgtt aatkgacaaa 360 tagagagtct tataagccct gatkgktctc gaaaaaaccc tkctcgggaa ctgcagagac 420 ctaaaawttc tbbcaccccg ndctctagag tggagaatac tggngtgatc ctaaccaagg 480 ctgtcgagat tggattgcta taaaagtatt ctgtgaca 518 121 555 DNA Murine 121 ggaattcctc tgtatagccc tggctgtcct ggagctcact ttgtagacca ggctggcctc 60 gaactcagaa atccgcctgc cactgcctcc caagtgcggg gactaaaggc gtgtgccacc 120 acgtccagcc ttgtttgtct atcagttcta cagcactcaa agataacctt ttgaaatcaa 180 tttgctattt gggtgacaca attcaatctt cattcagcaa ctgcaaacca attgagttct 240 tcatgccaac tcagaaatac atgattacta gcttttacaa gctgagcctc tctacagctg 300 ctggcaaaaa tggggcacag gggaggaggt gattttaaaa cctgccattc aaacttatct 360 agtctwamca gtagtcagag ggaaatatac ttgagaacag ggtaaaacca gctttggcca 420 cattaagttc atgttagtgt agaaaattta aaatcacmaa catcaaatct cagtctactg 480 tgcaaawtat aaagccgaat tttaccattt atactcagtt cttttggakt caatctcagc 540 aacatttact aataa 555 122 270 DNA Murine misc_feature (1)...(270) n = A,T,C or G 122 ggaattcggc gccttggatc catttccatc tggttctkct gagacgcgtn tngctccctc 60 cccgcaacag ccaaaatggt gaagctgatc gagagcaagg aagcttttca ggnnnvhcct 120 ggncgcngcg ggagacaagc ttgtcgtggt ggacttctcn nctacgtggt gtggacctnn 180 cnaaatgatc aagcccttct tccatnccct ctgtgacaag tattccaatg tggtgttcct 240 tgaagtggat kgtgatgact gcbrggatgt 270 123 186 DNA Murine 123 ggaattcgtg acttgtccag agtctcagcg ctgataaagg agaagctgaa agtcctcatc 60 tccagcagct tkgcctgctt cyagagtctg ggttcttgaa actgggaaag gaaatttcct 120 tctgaccaga agagtggaaa gggaatctgt ttgaactgga cagagtgggc agggtkggag 180 aggaga 186 124 452 DNA Murine misc_feature (1)...(452) n = A,T,C or G 124 ggaatcgacg cccaggctcc acaggtcgca gcgcttgtcg tagatgctgg cctcttcact 60 gaaggcctcc accacctctg gnbccatgta ctcagctgac ccacacgggg tgagcagctc 120 tggtgtggag atgggggagc agtctccatt gagtttgata ccactgccaa ggtcgaagtc 180 gcagatcttc actggcgaga cctggttggg gtgctcacat aggatgttct ctggctttag 240 gtccctgtng gcgatgcctt tgttatgcag gaagtccagg gsactggcca cgtcctgtac 300 taccacbsbg gsctccagcn cgttaaagtg gcgccttcta tggatgtggc ttaggatgga 360 tccgccacgc atcttctcaa acaccaggta gaaacggtcc tcctcctcaa agadctcaat 420 cagttctaga acattccyat gtcccccsgc ac 452 125 279 DNA Murine 125 ggaattccaa cgaacgcttt gccacactct gcacagacgt ggactctggg accgtgggtg 60 tgcagatgct ttctcatagc agagttatcc ctgaacatct ttgtgcagcc tttatgaggg 120 caagctaatt gttcttggag catcatcttc tttaattttt cttggcttca ttctggcaaa 180 ttctgccagt bbcttagggt ctgagaggtc aattggccag gtatccctyc caggdgggag 240 tttcttbcct gtcatatatt ccagaatwat caggaggtg 279 126 236 DNA Murine misc_feature (1)...(236) n = A,T,C or G 126 ggaattcgaa cgyyggcagt aaagcagtcg ctgctggaca aggtctgacc cccaccactg 60 gcccacccbs ttctaccaca aggacttbnc ctctgaaggc cagtggctac aggtggtagc 120 aggtgggctg cyctcacccg tcctggnntc cccccctcca scctcccttc tcagtcccta 180 atybgcctct cccaccctcn ccccaabcat tbcttcatcc ataagtbggt cccttg 236 127 362 DNA Murine misc_feature (1)...(362) n = A,T,C or G 127 ggaattcaga acctggcgga cgaggagccc tgggcagttg gtatgggcag tacaggaacc 60 atttcgactg tctggtcacc aagtttaaga gcaatctaat gaagtggggg acactgtaag 120 ctaactgaag atgaatgtgt ggkggctttt wctcaacaac cattccccta gagtctaata 180 taaaagtaga tttacatttg tgggtaatct gaagctggtg atttctagtg cctttggtaa 240 taatcaataa cncagcagtt gcgtggcaga kkgatccmcg catggataaa tacaaatatt 300 aaattagcat aattttttaa ctttttgtac aaatatacat gcttttttnc tttttctcat 360 ct 362 128 315 DNA Murine misc_feature (1)...(315) n = A,T,C or G 128 ggaattcttt tttttttttt gttttgtttt gttttgtttt gttttgtttt tgctttaatc 60 ataatcagcc cagagcattt tttgttaaca atgcctctgt tttcatgaaa gttcataaca 120 tcagggtttt taaaaaaaat taactaaggt gcttttagag ttgaatctgt gagttaccgt 180 cagcacacta gtgggctaag agtgagcagg gtgttttcag agaaacaakc kkcyccccca 240 nnncacaact tatcttttaa acttagaagt aacctgttgt hccccagcct gcyctttgtc 300 acctgagtkc ccaga 315 129 251 DNA Murine 129 ggaattcaat agatatttgc tagacttacc aattcaaawg ttttgttctt cctaggttgt 60 cagggaagta tcactactac ycttcagttc agaattgctg aagtaactga ttgtytgatg 120 atttgtgaac atgatcttaa ctatgtgact aaaatatcag atcattacaa tactkctcaa 180 ttgatggata catgttgaat atcagtgtat wctttgatgt ttttwattac ttkacycttt 240 ttttaaacct a 251 130 338 DNA Murine misc_feature (1)...(338) n = A,T,C or G 130 gaattccgag cgggcgagcg ccgggagggg gccgggagca gggcagctcg ggagaccgga 60 cggtagcggc ggcggcggcg gcgggctcgg cgccctcttc tctgcaagcc atgtttgcca 120 aaggcaaagg ctcggcggtg ccctcggacg ggcaggctcg ggaaaagtta gctttatacg 180 tctacgaata tttactgcac gtaggagcac agaaatctgc acagaccttc ttatcagaga 240 ttcgatggga aaaaaacatc acactgggtg aaccncctgg gttcctgcac tcgtggtggt 300 gtgtattttg ggacctttac tgtgcagctc ctgaaagg 338 131 94 DNA Murine 131 ggaattcaac agaatacaag aaatggaaga gagaatmtaa rgtgcagaag attccataga 60 gaacatcgac acaacagtca aagaaaatwc aaaa 94 132 323 DNA Murine misc_feature (1)...(323) n = A,T,C or G 132 gaattcgaaa aaggaaacgg aaaaattcta cttccgggtc agattttgac actaaaaadg 60 gaaaatcawc agaaacctct attatctcta aaaagaaahn ccagaactwc tcagagtyhh 120 ctaactatga ctcagagtta gagagagaga taaaaaccat gagcagaatt kgggctgcca 180 gaaaaagtdt tccagagaaa aaagaagagg actcttctga agatgaaaaa cagggcaaaa 240 aagtagtgga taatggaggg catgagaggg cgaagacmac mcmagaaggg tcatctgctg 300 atgacactkg tgacactgaa ggc 323 133 402 DNA Murine misc_feature (1)...(402) n = A,T,C or G 133 gaattcatgt caaacaggta gtcataacac tcacacatgg tttttdcttt ctcccatgtt 60 tctccccaca cgtacacccc atgayncygg acaagaacyg cacaggagtc tkggtactca 120 ttcatggcat gagccatcct ttctttkaga tccttctctt caggagtgtt ctcaataath 180 ggtwccacta acatatcatc gtatctgtaa tagcctcctg aggtacattt ccttattcct 240 ttgatcatct cttgatgtgt aattttaaac tcctgtcctg gaaacagaag ggtagccatc 300 acagcagctt tagagtgggt atgaatcact gcgccagctc cctctcatgg tataagcatt 360 catgaaaaga ggagtgcact ggcttttwtt cagcttctta ga 402 134 203 DNA Murine 134 gaattcgtga tcatgaagcc tagtgcgctc attacacaag ggggggggak gkctcaggac 60 ctctccaccc cgggagtcat ttccctgtgt tgctgtggaa ctaatttgaa aagtaaagtc 120 caaggaaaca ctgctctgtt tctgagacat gaagaaatga aaacacaaga caaagcaaag 180 agcgtgcgca ttctctggcc cac 203 135 87 DNA Murine misc_feature (1)...(87) n = A,T,C or G 135 ggaattcgtg atcatgaagc ctagtdnnyt cattacacaa ggggggggga ggdtcaggnc 60 tctccacccc nnnagtcatt thcctgt 87 136 342 DNA Murine 136 ggaattcgga agctccgccc cggctaaggg ggccagcatc ctggggcctg cacccatcct 60 gtacaagata ctgcccagag ggttccttca aggcctgggc agttcaaaca gccacactgg 120 acagacaata aataatgcag ctgctctctg gacagcctcc tgtgacctat ctcgtttcga 180 gccactcgag tttcggccag cttgctttgt tcagaatgcc aagccccggc tgggtttctg 240 gccacgtggg tactatggtc ccactgaggg ccagtctgag cctgcctaam aaaggctaag 300 taaggkggct atcctgaaga gaawgcccta cttactttga aa 342 137 341 DNA Murine 137 tgaattcggc caaacgactc ctgctggtct caaccccgta ctgccggggg caactagctt 60 ttaaacgcct ttctgggcgg tcagctacca agtgcctgaa gacctggtgt atgcagcgga 120 ggggcaagct gcctgggcca cttacgtggt aggtgcctac cacggggaca taggggctgg 180 agcggcagaa ttcgcttata ctggttggga gggtgggagt atccactgtg gctagttcac 240 accctgcttc ccctccccaa caagcacaag gggtgtgagc ctcaacccta aacaggcaag 300 trtatratcg ttttactctg ggcacacctg awtatggttt t 341 138 350 DNA Murine 138 ggaattccga gcggccgctt tttttttttt ttttttttaa aatctcagta ttatttaatg 60 agaacgcccc accctgccat gtacagggtg ccccgcactc gctactcacc caccatgtta 120 aggaaaagca ccaggaagta cagagggtcc tcatggctgc tctccagagt tataatttaa 180 aggtatttct ccatggtaaa actacaatag ttacatacca aggcaatact acatgcttta 240 catagtccca tgaaaaagaa ttcaattgag tctaatccct gatgcaaggc acttcaaagc 300 acccgcgata aaatgcccat gtaaacagca gtgcagttgc accttbccaa 350 139 156 DNA Murine misc_feature (1)...(156) n = A,T,C or G 139 ggcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt 60 gtcaagcatg acaaagttgc agccgaatac agtgatccgt bcngccctgg acctgttgaa 120 cgaggtcggc gtagacggtc tgacgacacg caaact 156 140 411 DNA Murine 140 ggaattccgc ttgacctgcc ttggggtatg ggtactgctt tgctttgggg tacagtgctc 60 cagtaaaccg aggtatgatc atgttaggca ccaacgagtc atttatcatc aggaaggcaa 120 gtctctctcc atcgggggac caccagtggg cgatatgaga atgcagaagt tcttctagaa 180 taaatgagtg ttattttaca tcaacttcat ataaccagtc agcaatccca ttaaaaataa 240 tgccttcctt tcctgaagat gttagtcgta aagaactgct cttgatatca ggttgatagt 300 agatattgtt ttcaaaaata taaatcagct gctgtccttg cacaccccag ggcgccatac 360 tgcaacactt gagttctcaa cttctggggg atthaacttc cacamyttcc c 411 141 557 DNA Murine misc_feature (1)...(557) n = A,T,C or G 141 ggaattcctc tctctctctc tctctctctt tttctctctc gctctctgcc tttctctgtc 60 tctactccct caactctctt ccccatgccc tgaataacct ctattctata ctacatgact 120 ggtccctcag ggggaagggg tgcctcagca tgggcccgca gaggtacccc cttccccaca 180 cctgatggca ccaaacatat tccttctctc cttctctccc tgctcatcgc ttgaggtagc 240 atggttctct ctgggaagct ctgggtgctg agtcagggct ctgctctggc cctcccctga 300 aactccatca gaatctacat ggccctggac tgtggcaatt tgcttcttgg accctaacaa 360 gactttaagt tyctygaagg gcaaggtttc ttcccactaa atccagcaca gggcaagaca 420 catagtaggt gttccacaag cacctaatga gtgctctggg ttgttgggat ttttttttgt 480 ttgtttgttt tggttttggg ktttgtttgt tggttagttt gtttagynsg ttttgcaaca 540 akgtctcaag tgacata 557 142 231 DNA Murine misc_feature (1)...(231) n = A,T,C or G 142 ggaattcaat catatttatt ggatcaacaa atctcctagk nccttttacc acatacattt 60 acwcctacta cccaactatc cataaatcta agtatagcca ttccactatg agctggwghh 120 gtaattacag dcttccgaca caaactaaaa whytcacttn cccacttcct tccacaagga 180 actccaaatt tcamctaawt tccaaatact taattaatta ttgaaacaat t 231 143 529 DNA Murine 143 ggaattccag acttgtgctt cttgatgtct gtttgatggg agctactgac aggcttaggg 60 ctcaaccaag tggcttgtat tctgaaaact tctacctggt tatgcatata attagtaaga 120 cacttagaat gagcctaatg tgagcctggt gggtggctgt cccgctgaga aaggcctttc 180 gcagtttaga ggcatctctg ttctctcctt tataggttgc ctacatagag aactgctgtc 240 ctttcatact gctctgttgt aaccgtttta tcttcagttt cattccttgt atcaagatct 300 taagcagcag cagttctcaa cctgtgggta gtacgcaacc cctttgggga ggttgaatga 360 ctctttccca ggggagcgta tattagatta tttacgttac gattcatagc agtagcaaga 420 tgaccwgtwa taaaatattt ttatggtggg ggggccacta catcargggg cgtacattaa 480 atggttgtaa cattwgcaag gttgagtact cgctccatct ttaaaacca 529 144 148 DNA Murine 144 ggaattcctc cctttgtctg cagtttttcc ccttgacatt cattcattca ttcattcatt 60 cattcagtga agagcttcgt gtycagtatt ccagactccg atgaaahtyg aaaatcgaty 120 cttctctkkt ctaattattg tctaatca 148 145 425 DNA Murine 145 ggaattcgcg ggtctaaaag ttcccaacac ttggagggct gggtgggggc cgaagctagg 60 gctgtgggaa cgacaacttc tgggtgtatg atgttgatgg tgagcgtctg ctgcacacct 120 actgtgtgcc aagcacttgt gcgtgttcta catactaaac ctcgtgacca tggaachvgc 180 tcattttccc aatccgtcga ccgaggaagc agagactgga tggtttggcc agbbtagagg 240 gcagtgggga ttggtttggg ctgaggtctg catctttacc ttctgagttg cagatttcga 300 agaagtatac tctgatctga gcacggcagg agggcagagg aggccaagcg gcaggcatgg 360 gtgcacccta ctgccatctg ggccggcctg gagaccagga ggctctgaac gtacacacga 420 acgcg 425 146 399 DNA Murine misc_feature (1)...(399) n = A,T,C or G 146 ggtgacacta tagaatactc aagctatgca tcaagcttgg taccgagctc ggatccacta 60 gtaacggccg ccagtgtgct ggcgcggatt ctttatcact gataagttgg tggacatatt 120 atgtttatca gtgataaagt gtcaagcatg acaaagttgc agccgaatac agtgatccgt 180 gccgccctgg acctgttgaa cgaggtcggc gtagacggtc tgacgacacg caaactggcg 240 gaacggttgg vggttcagca gccggcnctt tactggcact tcaggaacaa gcgggcgctg 300 ctcgacgcac tggccgaagc catgctggcg gagaatcata cgcattcggt gccgagagcc 360 gacgacgact ggcgctcatt tctgatcggg aatncccgc 399 147 345 DNA Murine 147 ggaatcttca cgttaccctg gaaagagagc tccagagctt gcatttaaac ttctgggcat 60 ctctgcttca atgcctttct aaccagtggc tctttttcgt gtgcggaaac ataaaccagt 120 gcacatccca catactgcca agaagtgaaa gggcttcata aggaagatgg gcaccaggga 180 ggaccctggg cttyctcctc ggacatgagc ttgccacctg kgtcatatgc tctgdaaggt 240 ttcttctgtg actgagacta gtaaacattt tattccctgc agagatgagc tgtctgkgca 300 tggggggtga cttcagtaga caggagagcc gacatgatgg cttta 345 148 67 DNA Murine 148 gaattcttta aaatcactaa tcgacctghc ghcctcagmt tagaccacat agrcaacttg 60 attattg 67 149 182 DNA Murine misc_feature (1)...(182) n = A,T,C or G 149 ggtgacacta tagaatactc aagctatgca tcaagcttgg taccgagctc gngatccact 60 agtaacggch gccagtgtvg tgngaatthn cgcatccacc aagatgngaa twhnacatnc 120 cttgtgaata tngaatgggn ntataccaan ggtnctcggn awtgrrsctc tttsctctta 180 gg 182 150 336 DNA Murine 150 ggaattcgaa ggatgccctg ctgaatcagc tgtgagctcg ggacggggca ggtggtgctg 60 ttgcaggcag ggacagaaat gctgggagga aggtgacaaa tagtgagctt aggcttccct 120 cggtcagtta cagctgcctt aaccctgagg cggagcaggg catgtgggtg gtgaacaagg 180 cagtggacca agcagagcgc tgccctgtga gaaagtgcag aggacagtac agtgacaagg 240 atccagaaca gggagcctga agtcttccac cgaaatggca tttggaggag tkkcttcaga 300 gaagcattta gaggaagcca gttggacaat tggcct 336 151 108 DNA Murine misc_feature (1)...(108) n = A,T,C or G 151 ggaattcgaa gcttcttttt gcaagagatg gtcattaaag acagttacaw ctggtcacac 60 aatgcatagg nccactgacc acaaagtgtc cagahccaat taatatat 108 152 607 DNA Murine 152 ggaattcccg gctcgagcgg ccgctttttt tttttttttt aagacttaaa attgaattag 60 tatttgtaca gaaaggtgca ggtggaataa ctccctccgg cctaggatca aagttatgcg 120 gagaattctt gatggaccct tcccctgccc ccagtggtgg cccgagttgt taagtgcgat 180 tggttagagt agattccagt cgggtcattg tggtggagga gtgggggcag tggcaggtaa 240 gggggctcag ttgctgcagc actggctccg gctggctggg ttgctctcct gcagatccac 300 acctctggtt cggcccggag ccccagccgc attctggggc tcattcttgg gaagcttctt 360 agctattgcc atgaaaattt cattcacgtt cattgcagtc ttggcagacg tctccatgaa 420 gagcaagctg ttgtcatctg cataggcttg tgcttcctga aactccacag ctctcttgct 480 ggccaggtct gctttgttcc ccgctagtgc aatgacgatg tttggggctg ggcctgcctc 540 tgtaactcct tcacccaatt cttagcccgt gcaaahgtat ctsbgttcgg tgatgtcata 600 gaccaca 607 153 520 DNA Murine 153 ggaattcttg ttttcctcct gagacacagc cttgaaagca gtctcctgcc tcagcctcct 60 gtgcagaaat tatagatgtg agccactgca cctggcttct aaaacttttg actatgtagg 120 gctctgtact gtcattcctt ctatattcat tgacaatgga ttcctggacc ccctaagata 180 tcaaaatcat tttctgaagt ggkataatat ttgtatatcc cctatacctg taacacccaa 240 tacaatatag atgtcatgta aacagttatt aagctgtctg tctagtttag ggtggaacga 300 caaggaaaaa aaggtatatt tagcacagat gtaatttttw aaaaatgaaa tgttttcaat 360 ttgtgattcg ttgaagctgt agatgcaaaa ctcamgggac attaaaagtc aactatatat 420 cattgggtga ctgatcttct ggtccattta aactttgaat tccctataac acaactcaaa 480 gagaacayga tggagagcct aggtctgtat ccaatcaatc 520 154 78 DNA Murine misc_feature (1)...(78) n = A,T,C or G 154 gaattcttgt ttthhtcctg agacacagcc ttgaaancag tctcctdcht cadcctccyg 60 tncagaaatt atagatgt 78 155 345 DNA Murine misc_feature (1)...(345) n = A,T,C or G 155 ggaattctcc tgctctggct cacctgtcct gctcggggct ccagctgatc tgtgctgttc 60 ctggtagcgc tgctcacgtc gggcagcctc ctgcagctcc cgctctcgtc gctcctcctc 120 caaccgctgc cgctcctctt cggcacgccg cttctcctcc aggcggcggt tctcttcttc 180 cttctcagct ttggbccaga agttatcctt gccgactctc ttgatctcag atatggcatt 240 ggtcttctgg tacacagagc ccactggggc ctgcbgccta catcctggaa ggaggtgctt 300 tccttatgga agctgtwgtt ggccccagag gccttngcaa ccttc 345 156 342 DNA Murine 156 gaattcctag gaaaactcta aatgaaagta aatgtctgcc actcactgcc ctcagctata 60 atccaaccag tgtactttct tctcatcctg cagaccagaa caagtcccaa agctctggca 120 atattaatac agcaagacaa gtaacctttt ttttttcaag tcttgaggat gaaccagaag 180 actttagttt aagataccaa gtcaaagttg cacgttaacc tggaccacag tcaggcccca 240 gahmvctggg agtgtggttc acacctgtaa ccagcactca cagaggacaa tgtgcctgct 300 gcaaacccaa gscagcttkc actgggagtc tgaccactga ag 342 157 369 DNA Murine 157 ggaattcgct gagtctaaca aatgaggctt atagtttggt aggagttaat aaacttctta 60 gtaattatat attgactgtc tactatttat atgccaggtt actctgtgga gattattggc 120 aaatctagaa gtgaaattgc tgactgggtt tttaatatag taaggaaaat gacatataca 180 cataatagta ttaccaggca atcaaagata gatactaatt cagtgatact tagaatcagg 240 ggaggcattg cttttaatag gtgaggcaac tgggccttca gtgatgagta atgaggaaca 300 atatggratt ccgtgcagca gaaaagaagg tatmgacatg taggtkagga aaactgcmgc 360 agtgtttat 369 158 285 DNA Murine 158 ggaattcccc ggctcgagcg gccgcttttt tttactattt ttattagata ttttctttat 60 atacatctca aatgctatcc cgaaagttcc ctataccctc cctctgccct gctcccctac 120 ccacccactc ctgcttcttg gccctggcat tcctctgtac tggagcatat aaagtttgca 180 ataccaaggg gcctctcttc ccagtgatgg ttgactaggc catcttctgc tacatatgta 240 gatagagact catatctaca tatgagtctc ygggggtcyt cgtta 285 159 443 DNA Murine misc_feature (1)...(443) n = A,T,C or G 159 ggaattccat aagtactatt attttattaa aaattttaag ttgaggctct aattagacat 60 cagcctgatt tctttgagtt ccacacacac acacacacac acacacacac acacacacac 120 acacactgtc ttcagcagtg agaccttaca atcacttctt agaaaacaat tgataagtag 180 ccttgccaat agccagtgtt attttgggat tccatgggat ttcatggagt caacattggt 240 cagcaactca attagatgta agccattcct gggactgaaa ggtttccttg gagaggaaag 300 atgtctagtt ggagtactgt ttcccttgtt gtttagtgac tccatttaga tttaatcata 360 tatgtatata ttttaagaag tttcaactgt agtaggtttc catatggacc ccaaaanntc 420 ttagtgctaa ctgtccctcc ctg 443 160 239 DNA Murine 160 ggaattccca actcccatct cgctgagggc tgtgccatgg gctcctgtaa ccttgctctg 60 ctcttcaaca aagaggacca gtgggaggaa acttgtgggc ccagcattcc caggctaagg 120 aactgggggg gagggccagt tggatgatcc ccagggtatt aaaacctcac tttggagaag 180 aggcagagct gtgtttagaa agkcaggkca gatgtgggaa gagcattgca actbcaggg 239 161 346 DNA Murine misc_feature (1)...(346) n = A,T,C or G 161 ggcgktaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc 60 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca 120 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcsccggc tgctgaaccc 180 ccaaccgttc nnccagtttg cntgtcgtca gaccgtctac nmcgacctcg ttcaacaggt 240 ccagggbcng hahcggatya ctgtattngg ctgcaacttt gtcatgcttg aacactttat 300 cactgataaa cataaatatg tycaccaact tatcagtgat aaagaa 346 162 218 DNA Murine 162 ggaattcccg gctcgagcgg ccgctttttt tttttttttt tttttttttt tttttttttt 60 tttttttttt tttttttttt cataattgat tattttatta agatagttgw ttaataactg 120 aaaaccagag gtaaagtaac aaattccaaa ggctttttaa aggcataawa tttwaaggct 180 attccaaatc ttcttgggat graagaaaaa tccctttc 218 163 309 DNA Murine 163 ggaattcacc cggctcgagc sgccgctttt tttttttttt ttttttcccc tccttttttt 60 tttttttaaa ggaaaaccag tcaaatcatg aagccacata cgctagagaa gctgaatcca 120 ggtcccaaag gcgctgtcat aaaggagcaa gtgggacccg cacccctttt ttttatataa 180 tacaagtgcc ttagcatgtg tcgcagctgt caccactaca gtaagcyggt ttacagatgt 240 ttcccvavcg gaattccacc acactggcgg ccgctcgagc atgcatctag agggcccawt 300 tcgccctat 309 164 425 DNA Murine 164 ggaattccat attccagcct ctaccaaaag tgctggatcc tgatttgtgc aatactaggg 60 actgaaccct gatctttgta taaactaggc aaactatcaa ctgataaagt gcactgggat 120 cttggaagtt ctgtacttgt gattctggac ttttggaagt cagagaattt taattaccca 180 gtgagtcgac tgctgctact caaaattttc attagtatct acgtgggggg ggggggctta 240 gaaatgtaaa cmtggggagc tggagagatg gctcagtggt taagagcact gactgatctt 300 cccatgtggt ggctcacacc attttttwat gggatctgat gccctcttct ggtgctgtct 360 gaagacagcv tcagtgtaca tatataaata aaagaaatgt aaacatgcmg cttgggaagc 420 aagta 425 165 358 DNA Murine 165 ggaattccgc gcgggcacgg agcaggacgg cgggacggcc ggccctcccg gccggagccc 60 gcgggcgcgg chgcggggcg gtggcccagg gcaggcgcct accccccccc ccccccagca 120 gcatgtcatg gtttagtggc ctcctggttc ccaaagtgga tgaacggaaa acagcttggg 180 gggaacgcaa tgggcagaag cgcccacgcc acgcgaatcg agccagtggc ttctgcvcac 240 ctcgctacat gagctgcctc aagaatgcgg agccacccag ccccactcct gcagctcaca 300 ctcggtgccc ctsgcaggat gaagccttca tcaggagggc gggcccgggc aggggtgt 358 166 376 DNA Murine 166 ggaattcgta caggttgaac agaattgaga atgccttgaa gacaatagag agtgccaccc 60 agcagacaga caaactgaag gagctttatg gacaagtgct gtaccgcctg gaacgctacg 120 atgagtgctt ggctgtgtac agagatcttg tccggaactc ccaggacgac tatgatgagg 180 agaggaaaac aaacctgtca gcggtcgttg ccgctcagag caactgggaa aaagtggttc 240 ctgagaactt gggtctccaa gaaggcacac acgagctctg ttacaacgct gcatgtgcac 300 tgatagggca aggccagctg acccaggcca tgaaaatyct gcaaaaactg aagatcttat 360 gtcgccgtca ttttca 376 167 250 DNA Murine 167 ggaattcttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 60 tttttttttt tttttttttt tttttttttt tttttttttc ccaaattgtt ttgatcctta 120 tagatttgga gggccaactg catttttcat ttatactttk kgcagggtaa gtactttaaa 180 aaacaattaa ttgrcttaaa tccattaaca tttwtgtaag ggattatatg gtcagccatt 240 ccttggtata 250 168 392 DNA Murine 168 ggaattcgga aaatgttagc atttaattaa cctccggtgt ggcttttaag ccaccagaac 60 acaggcacct ccaacaccct taatcttctc ctcagctctt ctgctgaaga atttggcctt 120 cacgatgaca ggttgcttag ggagctttcc cttgcccaga actttgtagt agcctgatcg 180 aacaacatca atgatgggag caactccagt cttgttthtc mgcattgacc cgtgtctgch 240 cgctgaccaa tgtccacagt ttatccaggt tgactgttgg gcagaagctc tggttcctct 300 tcaagtggta atgccgcata ccaactttcc caaagtaacc tgggtgatat ttgtcaaagt 360 tgatcctcgt ggtgcatgcc tccagcattc cc 392 169 387 DNA Murine 169 ggaattcctg aaggctgagg ctgtgaagaa ggaccgcaga aagaagctga cccagtccaa 60 gtttgtgggg ggtgcagaga acactgccca ccccagagtc atccctgcac ctgagatgag 120 acaggaatcc gaacaaggcc cctgccgcag acacatggaa gcttccctcc aggagttcaa 180 agccagccca cgcatggtgc cccgtrctgt gtacctgccc aactgtgacc gcaaaggatt 240 ctacaagaga aagcagtgta arcccwcccg tgccgcaaac gtggcatctg ctggtgtgtg 300 gacaagtacg gaatgaagct gccgggcatg gagtacgtgg atggggactt tcagtgccac 360 rccttcgaca gcagtaacgt tgagtga 387 170 226 DNA Murine 170 gaattccctg gagaagcctg gagctccaca tgcagagaaa tgatctgtcc ttgtgtctcg 60 ttctgattaa aaacaaaaac aatcaaataa aaaacaaaat kgaacaacaa ccttagtgta 120 tggcatgaga atgtgaaaac actagagatg atcaggggga tcttcaaatg gaggcagaca 180 gccagtttct gaagagaatt gcagtagctc ggaaagccag tcaccg 226 171 440 DNA Murine 171 ggaattcgca gaggcaggca gatccctgtg cgtttgaggt cagcatggtc tacagaggga 60 gttccaggac agccagggct gtagaaaaac cctgtctgga aaaaccaaac accaccacag 120 aataaaacaa ggagaaacag acttgtttcc aaagtggctc ttctgaagcc cctgctctga 180 aagttcacgt gaccacagcc atgccccctc ttcatctgag tcactggctt aaggcaaggc 240 tgcgccgaga ccatgagacc gtgagaccag atggtggtgt gacatggagg gaaggcggag 300 gtctggctgc tgtgcagccc tagcsccagt ccaagagcac ctggtcttcc gagtcagcct 360 aggtcagtgg tagtcatcaa gctcacttct gagcagggaa agatccagag cgccaarccc 420 agccccgtcc cacagatcca 440 172 449 DNA Murine 172 ggaattcgtt tgaattcctt caactacact cagagttcaa gtgcagacac actgtgtccc 60 aggctcccgg ttcctccaag ggatgacaag tgtgtgccaa tacctccgac acaagttttg 120 gcacaagttc cttgcactca atactctcac aaggcgagca cttcactgcg gactaagcta 180 taccacagcc ctgagaatgg aatttttcca aggtttccat ttagagttgg atcaactgtc 240 ctctctctgt cgctgggatg acatgagaag cttacagggt ggcacaggtg ctgaactcag 300 tgctgatttg tggcgctctc cctccttctg cttccttttg taacctccgg acatgtgctg 360 gtccsctgcc cctcacagta gggtctgcac tgtaagtatt gtcttataga ggagaagact 420 gatcagggag aggttgagca agcagaaac 449 173 401 DNA Murine 173 ggaattccag gttattattt tgtttttggt gttttgtttt gtatttttgg agataaggtc 60 tcactatgtg gccctggctg gcctggaatt tacagaggtc agcctgcctc tgcctcttaa 120 gtgctgcaat taaagtcctg gactatcact tcaggccctc tgaggtcagt tttaatcagc 180 ggaaatactt ttatcattct ggctttgctc ttcccagata cctacactct ttcttcactg 240 atactcaggs ctgaaccaac ttttatcatt ctggctttgc tcttccgaat tccaccacac 300 tggcggccgc tcgagcatgc atctagaggg cccaayccgc ccctatagtg agtcgtatya 360 caattcactt ktcgtcgttt tacaacgtcg tgactgggaa a 401 174 369 DNA Murine 174 ggaattcccc ggctcgagcg ccgctttttt tttttttttt tttgaaagtt tcagatgttt 60 ttattcaaag gttctcaaaa gaaataaaac agaaaaagct aacaatctga tcaaatgtac 120 agttcaaaaa tgtcttttgg cgtttaacaa gtcctaggaa agaaaactac agagtcatct 180 tgaaccggta aataagtcac cactggcaag tatgtagcac tagtagaaca aaaataaaaa 240 attaactctc ttgatcatat agatatctct atgaaaatct tttttttcaa tctgtacaaa 300 aggtctttct tcataaatta atttttttta taatttaatg gctgtctacc ccggctcgag 360 cgccgctcg 369 175 367 DNA Murine 175 ggaattcata attaatagca acaaacggcc gtctcgctgc ctgccgcagc cgcagggtgc 60 ttttgcagac ctgacgagca atttttgtga aatacgtagt acgaaggaag aaagcttggc 120 gggtcttcac tgcagacttg gggcttccgg tgttccggac cggcatgccc tgcaaggcct 180 gccgggacat gtggcttctt gcrcgcgggt cctctgcagt cgggctggga gacttctctt 240 cgtctgactg ggtaggcatt ttcagacctc catacttttc caatacagcc aacaggtcgc 300 vcagagtcta cactgcatgt taggtgggcc ccaggaatac cactgatgag actgtgtggc 360 gtasagc 367 176 387 DNA Murine misc_feature (1)...(387) n = A,T,C or G 176 ggaattcaaa gaggtctgct agccggtaga catcaaggat attctcctca tctacccatg 60 acatgaggaa atcacagcag aagtggataa tttctggtat ctgaagttgg caggcagcaa 120 ccagggtctc ctgcacattg ctcaggctga gctctagttc agaagtgtat atgaagtgca 180 ggatttggca catggcattg taagacacac cgtggatcaa gacctcttcc wgctccawct 240 ccttcaatcc cccagcaaac attcctctga aataatcaca cgatgcagct agcagaatcc 300 gatgggcctc aatgtgcttc ccctcagtga ccaggccaag tacctgaatc ctcttactgg 360 ggaaathgga amaatttmnn tggcttt 387 177 514 DNA Murine misc_feature (1)...(514) n = A,T,C or G 177 gaattcgttt tgcctatttt catgtgtaaa ttcattcaag tgatacaaga gccctaaaaa 60 tcaacccttg attcatcaaa aaatatttat ttaaaaaaaa gagagagagg gcccaggcat 120 ggtagctcac acatgcttat aatcacacac ttgggagggt gagccaaaga actgccatga 180 atgtggagtg agcatggttt aaaattcaac cctgtctcca aaacaggaga gggaaggggg 240 tgggagattt gaaaattcat atacaggaga aattaacaga caatattatc agaaaaccaa 300 agtacactta aaactgcacc atcactctgg ttcatcaggc cagagtgaat gcttgtgact 360 acactgtcgt ccacctgctg aggatgtact tattctttac tacaataact tctaaagtat 420 nctcatagtt hacagcaakk ccaganccta ataattatct aatctagngt ttctcaacct 480 tngcgatcac aaataatcta tgtactaaga cact 514 178 99 DNA Murine 178 gattctttat cactgataag ttggtggaca tattatgttt atcagtgcat aamgctgctc 60 aagccatgca caaagctgcg ccgcgcccga atvcvgtga 99 179 357 DNA Murine 179 gaattcggca aagggaagac acctccagct cagcccagaa gcaaagctgc tgagggggac 60 gtggtaccag gtggggctca gcactcatcc tccccgagca gggcatacgg gtttcgggct 120 gttaggcagg acccaggatc tgaagttggg gtgtcctcat ctccaaatcc ctcttcatct 180 gcatcccggt cctcctctcc ttactccwca caggagctgc tcagttcctc ctcctcttcc 240 tcctmmtcat cacctgccgg ccccaccctg ccctgcgaca gaccagctct gcagtctctg 300 ggtgagactc ccaggtgcct ctctgttcgc ctgtaaccag gagggtagaa acatagg 357 180 554 DNA Murine 180 ggaattcggg gagctatggg taggaagtgg tcccagagag gttttaggtg gaagaatcag 60 gaggagtcac aggtcaactt gcagaattac tgaagaatta ggaccccaaa ttttatgcca 120 attgatctat tcccctcttt ttatttctgg ggccggtttt ttcctttttt tttttaatcc 180 ctccttagct ttttatgcgc tcataatcaa ttgtacccat tccctacata acgggagcag 240 tgatcaggta atgaatgcat cgagccatca acaccagcta gagccatcaa caccggctac 300 cacaatgtcc tgctctccac aaccttgatt tttttttttt tatctctctc tatcgcttgg 360 cctgagttgg gagtggagtc tctgtggggt gcggccacdc acccacagag aaataaaagg 420 aattgagaag gtcgctacct ggcctgactw ctggggacag tgctggtccc cagaagttct 480 gaggagtgga ggvggcgtgv gcacgatgtc ccctcacggt gttaggaagg ygctcggagg 540 ccacaaaaga tggg 554 181 498 DNA Murine 181 ggaattcctt aacactaata gaaataaatc cattaaaatc tttgaaagaa agaaaagaaa 60 aagagtgggc tgagactcct gctaacctct gacctacact gacctgactg ctatggccac 120 tacatattca gtaacaaact caaaaccttg aggaaccctg tgctttcagg cataccatga 180 caagctagca tgcccaaggc cctgtgcacc atctccaacg cagaaagata agagatacac 240 ttacatgttg gcaggatctt tagtattacc accaggtcag ccacattgtg tcctgtagtc 300 attgttccct ttttatatga tcctacctgt ccggacttct tcaatttgca ctttcaaatg 360 ttcctcgggg gccacaaatc aagttgtcaa tcacattgtt gattttttgt caccaaagaa 420 aggatggaag cctgctcagc agaaattatg gggcaaggtc ttgattcctc tttcagcaag 480 gcttcacctg aaaggagg 498 182 461 DNA Murine 182 ggaattcttt aaatatgact atggccaggc agtggtggtg cacaccttta tcccagccct 60 caggaggcag aggcaaggag gatctctgtg agtctgaggc catcttggtc tacagagtga 120 gcttcagaaa aggcaaggat acacagaaac cctgtcttga aaaaccatac ataaacatac 180 cctctggccc ctttcttctc atcacgaaga aatagggagg gtacataaat tgtttagatt 240 tagcttagaa gtttatttac atgtctacga gtgctctcct gtggagctca agagagggtg 300 tctgatcctc cggaagagtt acaagaaggc tgtgagctgc cacgtggctg caaggaacca 360 aatctacttg gtgttcttgg gaacaccagt aggtaaatct cttaattact mgagctatct 420 ctccaggctc ctagattctc aggaaaaaaa cctgactaat t 461 183 477 DNA Murine 183 ggaattcgta ggggtggctc tgtccagtga gccaatcatt ccttaagacc cttctgaccc 60 ctcctgtacc atcgggactt aatcaccagt ctggggaggc attagggaag gggcaagggg 120 tgcagaggtt aaacctcagg agaggaactc aaaacccttc aatggggcta tgtgatacgg 180 agacttcctg ggatgtgtca ctgggtaatc aacttaaaag cttccttctg gttcttctca 240 caggctagcc tagaaggaaa gcttttgcta ggtkgaggtc tkggggaggt cttagtggtt 300 cctaatcccc tttctttgcc tttactgtct gtcatgcttg tacacccctt thagagcccc 360 amcccccahc ccctkgcccc tgctctttgg tcttctctgt gggaacctaa cyttgagaaa 420 acttgtgtcc caaattggca tttgctcagg gatatctsaa tttatktctc ttccagt 477 184 420 DNA Murine 184 ggaattcaaa ccggctcgcg cgccgctttt tttttttttt tttaatgctg ttgtttatct 60 tatatatgat aaagtaaatg tctttattcc tatgttgttg aaaactaccc agtaataatc 120 ctggagttca ctgtgtcaga ccttggagga gtgggcaaag agcagcagca caatagtgta 180 tgttgtgttt aggttggaag ttctaatagg caagtcagga attcttatat ctgtagctcc 240 tccagaagcc ccaggcacag gcggggctcg gtgtgagcat gtgcacacag cyccacccct 300 tcaccccacc cccdyhycag ccaggtgttt agtgcactga gatgtgaaga ctctgcttag 360 caaccagcag taagtcctgt ctcaatcgat gctaggtcgc tgtgagttaa gacagggact 420 185 301 DNA Murine 185 ggaattcctg aggacatgac atccaaagac tactactttg actcctatgc ccactttggc 60 atccacgagg agatgctgaa ggatgaggtg cgcaccctca cataccgcaa ctccatgttt 120 cacaatcggc atctcttcaa agacaaggtg gtgctggatg tgggctcagg cactggcatc 180 ctctgcatgt ttgctgccaa ggcgggggcc cgcaaggtta ttggggattg agtgttccag 240 tatctccgat tatgctgtga agattgtcaa agccamcarg ttagaccatg ttggtgacca 300 t 301 186 458 DNA Murine 186 ggaattcgtt cagcagtcct ggagactgag ccctcaactg agggcatctg acattctctc 60 caagttgaag gtctgatgca aaaccaatat tttgtttggt gtgtgagtat atatccccac 120 actttggagg cccgcagaag taacctgtgt tggagaaact gactctggtt tttacttaag 180 aggaaaaggg ggagagaaac tagtgatgtg tttccctgat agactttata tcatataata 240 taaatcacac atggggaata ccaaaaggca aaaataagca agccactgtt acctaactca 300 gaaaattata ctcttcatcc attttaggga tgaaaacaat tgctgtcaat ttacaagcca 360 actttcaagg cagaatttag gttatccaat caggatttag aatatcgaac atcttcaata 420 tctaaattta tattatatvg tcacaaatat caggaccc 458 187 502 DNA Murine 187 ggaattcgct ttttaaggaa tgctggtggt gcctgggtag ataattacat cacttgttcc 60 actgtgttga cactgttttc ctcatggatc tcctccattc ctagctttct ctgctatgca 120 ttttcttcac agcgcagctt gcggtccgtt gctgaaaatt ataagctctg catagtgttg 180 gctttactgt gatgacatgt ttcttctttt ttagctggcc cacacctttc tagggtccaa 240 ctacaggata gattacagac tttccattag tgtctatttc ttttactctg tgtagacttt 300 agaaagtcta atcaatccag agatgggcca attcagaatt gactataatt gaacacctgc 360 taaaagtatt tatgggagga ttgacacaca gcatgagtta tttgactttt gtaggatatt 420 taaaavtcat ttgcagttca tgtaacagtb gtggtcttaa aattcacata ataaagcagt 480 cctgttcaaa aaaaaaaatt tt 502 188 400 DNA Murine 188 ggaattccgc cctttgacac tgcaacagca tggtcatcta caagtgccaa gctgcattcg 60 tagctgtcct gagacctgag ctgtcatgtg acccttcaat ggcaggctgg acacactatg 120 aagggtaagg tccaaacttg gtccagccag taagaaactc acggaaaatc tagcttcaca 180 acaggagctc aaagaacctt acatactggg catttcacat caggcacatg tctggggaga 240 ggactggata ccagacctta taatcagcct aaacttgcta agaacaataa ttaggtccat 300 tttaaagagg ttctagccac tattcttgaa actgatttta ctaagtataa atcctcayyg 360 aaatctgttc taaaataggt tattgaaagc aactcctgtc 400 189 463 DNA Murine 189 gaattccttt gcttgatcaa tatgtttatt gtctttatga aaaaatcttc atagaaaact 60 gctttagctt tcagcagccc tttcctgagc tctgaggaag cttgccttct tttgagcaac 120 ccgatctttc ttctgggcaa gagacatttt gggacgattc cacctcttct tcttcacttc 180 tctcttgggc ttcttctcat agactggatt ctctcggata gcagcatgag ctttcttata 240 catctcctcc atcatgtctg gagttacgtt gttcttgatg tactgagaga actgtttctt 300 atacgcatct tcatcttcct ccattaggta gcgcatgtag tctgccacat tctgacccat 360 gatgtgcttc cgatgtacct ctgcattgaa ctccttgcyt tcagagtcat aaccagggaa 420 tygtttggta ctatgaggga tagacaagct tccathcaca rgt 463 190 188 DNA Murine misc_feature (1)...(188) n = A,T,C or G 190 ggaattccgg cttctgagca gatcagactc tcctcgttvn cgcastcrcd cvgctccttc 60 cagcaaccat gtctgacaaa cccgatatgg ctgagatcga gaaattcgat aagtcgaagt 120 tgaagaaaac agaaacgcaa gagaaaaatc ctcnrcmttc aaaagaaaca attgaacaag 180 agaagcaa 188 191 276 DNA Murine misc_feature (1)...(276) n = A,T,C or G 191 ggaattcctc tgacctcgct gtctcttctc tcctctcctc tgctctacct ctgtctcacc 60 tctgtcaagt tctatgaatg actgatagaa agctagtctg caaccattcg gcaggtagaa 120 atttcccctg ctctgcaggg agacataacc ctctgtttgg cgatggagaa tgaggagcag 180 agcagtgagc ccctggggag gctgtaatta agawccactc ctgnctgagc ctcgsgcaga 240 gcctcactcg sgattctccc tgtaactccc caacac 276 192 608 DNA Murine 192 ggaattcgga attcctttaa actacaagga ttttatttta ttagaatcta gcctgagcca 60 gaacctttta tggtcacagg aagagatagc aagtagattt actgacatca agaaggactg 120 cccagtggtg gagccagcat ttgaaactgg actatagagg accaactaca attgtgactg 180 catttgtgac tgaatgtcac aaaaactgct gagaggcttg tcatgtatat gagagacagg 240 gaaagagtca tagtcaagac tggaagcatg agcaggcaag aagtgatcct tagattctat 300 ccccatcagt tctttcacat cacatgtgtt tggcctctgt ataataccca gctgtattga 360 ccaggacttc tctgtcctgc tttgctcttg aattttcata gtgagcctac cttttggtaa 420 tgactattta tgagatagtg ttctattctc aggttactac tgtggattga acccaacatt 480 acaaacacca gctcagcaam gaaaaataac caattactth gtctctgttg aacattgaaa 540 acacttccac tgaaagaatg gagtgattaa aaaaagatcc macmgatgac cmaagtaacc 600 acagatat 608 193 278 DNA Murine misc_feature (1)...(278) n = A,T,C or G 193 ggaattcaca agatctacca cttacagagc aaagtaccca ccttttgtwc gaatgcwggc 60 cccagaagga cgaccctgaa tatacacgag aaaamctgga atracctacc cttacdgcag 120 aaccgttatt actaatgagt acatgaaaga agattttctg attaaaattg aaacctggca 180 caagccagac cttnacaccc aggagaatgt gcataangca kmggaggcct gasrgcatgg 240 aaacatgtgg aagctatata tatagacaat trctgatc 278 194 488 DNA Murine misc_feature (1)...(488) n = A,T,C or G 194 gaattcgaga gagagagaga gagagagaga gagagagaga gagatctagt tgtcaattga 60 acaaggtgta tttgagcctg gaggcatgag cagggctggt tcctgcggac cctgtgagga 120 ctgtgggatg ggcatgggtg ttgtctatac tgtggttgag caccagtgcc cagcgccagg 180 ctgactgact agctgatacc tccttggtat ttgcagggta ctcttgagaa gttcaggcag 240 gtgaaagtct gtggcatcct cctcattggt cttctgccct caccatcccc catgtaacca 300 aagagactct gagcvcctat tttccctccc tactgagaat ccctctggac tccanntcac 360 tcagggtaaa agtccatcct ttccatgacc actgggtggg tcttyaccat ccacnctcat 420 cacctgtctg aattagttga cgctccctct gcwccagccg caatgggctc agcctttgca 480 cgtggtat 488 195 523 DNA Murine 195 gaattccagc agttaagagc actgactgtt cttacagaga tcctgagttc aattcccagc 60 aactgcatag tgactcacaa tcatctgtaa taggatctga taccctctgc tggtgtgtct 120 gaagatagtt acagtgtacc catatgcata aaatgaataa ataaatcttt ttaaaatttt 180 tatttgctta attttatttg aatgtgtgtt ttacccactt gtatgtcttt gtatcacctg 240 cctgcctggt gactgaggag gctagaagag ggcttcagat tctctgggtc tagagctaca 300 gctggttgct agtggccatg tagatgctgg gaatcgagcc tgggttctct ctgaaagagc 360 aacagtgccc ttaaccactg agccactaga cataagcatt cagagaggat ttgttgttgt 420 tgttgttttg ctttgttgtt gtttgatttt tgtattytgc cacagtggct gcaaacattg 480 aatctgagtt ggaggtaatc cttttatttt acagaatmtc ast 523 196 480 DNA Murine 196 ggaattcccc ccgccatgac tttcaaacct gttgactaca ctgtagtcct ccttggaata 60 gactttcatc actgcttggg tctcctcctc tgtacttgca atgcccatct ttaagtcctg 120 catagcagcc aaagtgtcaa gacaacccag gatatgcaag gctgcgtgag atcgggtggt 180 aagagccctt gatcctgttg gcagagcaag ttcaggactt agaatactac atctggactg 240 catgtctgtt gcagagggaa gtctggcatc agcaaccacg gcattgtaac accagagctc 300 tctggtgctt ggtcgaaacc tccaaagcac atcatataca ggatcaagac acacaccaaa 360 tycttgcagg tcttcttgtt cagagtcatt gaaagtttta caacttccat caactttatt 420 tatcagaaga catttaaatg gtggaggtyc tgatatggaa gcaggamcca rggcctatta 480 197 424 DNA Murine misc_feature (1)...(424) n = A,T,C or G 197 ggaattcgca acacctctta gggcaggtgg caatccaaca acaacaaggt cccggagtac 60 agaaccaggc tctgggtcct aagcctcagg gccttctgcc tcccagcaac caccagggcc 120 tcctggtcca gcagttgtcc ccccagcagt cccagggatc ccagggcctg cttggccctg 180 cccaggtgac agtgctgcag cagcagcagc agcaacagca gcactctgga gctctgggtc 240 ctcagggccc tcacagacag gtgcttatga ctcagtccag ggtgctgagc tcccctcagc 300 nggcacagca gggtcacagc cttatgggac accggctacn cncnncccag cagcagcagc 360 agcagcagca gcagcagcag caacagcaac agcvgcagca acaacaggca acaacaacaa 420 cagg 424 198 455 DNA Murine 198 ggaattcagc ttacataggg aattctaggg cagtgaggga gtttgtctca agaggaaaag 60 gttaagtgtc tgaggaatga ccctggaggt tgtcctttga cacctgtgca ggtgcacaca 120 cacacacaca cacacacaca cacacacaca cacaggagcc aggtatggta ggtagcacaa 180 gcttgtagtc acagctacat gggcaggtga gactggatga tttagagttt gaggctagcc 240 tggcctacat ggtaagttca aatccagcct tggttatcta gttgagttgt tatctcaaaa 300 caaaacaaac ttatccacct atgtgagaca atgtgagatt ttttctctgc tcaaagacaa 360 atgtttttct caaaggtagc aacaggctga taggaacact cttcccagaa gagtdcacac 420 atgagchggt gcmctgggva tgctcagaag aggct 455 199 410 DNA Murine 199 ggaattcatc agaagctcat tttgttattc ttttttttct ttttttttta caaatcagta 60 aagcttaaag ccagagactt atagattggt tcaaatataa tcaacagtaa gatacagaca 120 acaagagata cagctaaagc cactaacagc aacagattca aagtaggaag atgggcaaag 180 gtcttatcag gaaaatgcta atgaaaagaa agctagatcg caatggtaac atcagataaa 240 ggggaaagca agccaagcta cattaaatag gggtaaggat ggcttcggtt agccttccaa 300 crcgtcacta taagtttgtt tctcacttwa ctgawctcat ctagctcctc cacaatctct 360 aaacagatca tcactrctca agarcmtgtt gtgtatatac ctcctgaaaa 410 200 452 DNA Murine 200 ggaattccat ggttaaagca tatcaaataa atactaggca aggagtttcc tgggagagtt 60 agaaattaaa aaaatttacc aattttctgt ctctgtgata attcaatgcc agtaagagaa 120 aggtattgaa gggacaattt tcatactaaa aaaagaattt ccctagtcat gtcaccatct 180 cttataaaga atccagggaa tcccagaaat agaaaattag tttcaggggg acccctgagg 240 cactttaaag cctttaaaaa attacagtaa taataaatta gctattgctc ttcagaggct 300 cacggaacag ctaacacaac aggaccaggt ccagagttag gtccgtatct caggttctcg 360 agctgcccgg ccctctttaa agcttagacg aatttccaaa tacaagacat acaatttaac 420 acagactgag tgggdctttt tgtttagtgg gt 452 201 387 DNA Murine 201 ggaattccat tctttcaaaa acaatgtatt atcacctgag aaataatcca catttagtta 60 acttttcagg gaacttctga actcatcata catactccac tacccaatgt cgacactcca 120 tttccacctc agccagttaa gtgtaaagta tgcaaaacct caatgagttg tttctaactg 180 acagactgca gagataaaag caatgacgac ggccttcaga tcttagcaaa aacaactgct 240 aaagtgacta tcaaggaaaa gaaccatttt agaagcagtt ttatgtacca aggtggttaa 300 aacttaaaat ttgacaggca gttggtggca cgtgccyttw atacccagca cctgggaggc 360 aaaggcmggc aggatttctg taggttc 387 202 278 DNA Murine 202 ggaattcagg gagagcgcag acaggaaaac tgcagaaagc cacagggaaa gtacggtaca 60 gactcagatc tttttatttt caacttactt ctcgtttatt tccccaccac tcctctggct 120 cctgcctaac tgggtcgcgt tggggatgtt tggcatggcg ctcttagctt ttgttcgttt 180 taattccgcg cgccccctth ctctcvggcg gattactagg tcccgaactc tgccactaca 240 accttaggag cagcaagcty cgccaactgg caccaccg 278 203 591 DNA Murine 203 gaattcattt tattttattt ttatttatta atagtaacaa aaatcagaag taacaaaaaa 60 cccagttaaa tggaatacag aagcacagca aatacaaatg caatttcaaa accactcggc 120 acagaaatct gttgaaacca ttttctgaag tttaactatt taggtcatag gactaaccaa 180 ggcattcgga gtgctcacat ggatttggtt gccgatggag gagcctgctt ccccaagact 240 gacagtagta cccaagagtc ctggtatatg tatgtgaaaa gacctccctg ggtcctggat 300 cttaagagac actgatgtta ataaaaccac caggaccaca taaaaccaca gaacaaaacc 360 ccagagcaag cccagagagc ttgccgtctt gttctatagg cttctagagg actctaggaa 420 ctgaagaaga tgtaatcctg cgtgttggtc ccatgcaaat ctcaacccaa gtctcccaaa 480 ccaggctact tagcagcttt tcatgaacgg ttcaaggatc acctgaatct atgggrgggt 540 cacctgaatc tatgggaggg tcacctgatc tattggtsch tcagagcaac a 591 204 578 DNA Murine misc_feature (1)...(578) n = A,T,C or G 204 gaattcgatt tattgaagca gtaacaagtt ggtcagatat ttactggaaa aaagcagttt 60 taatggtatt caaaaatact ttaaaaagta ttctagcaca agatttcttc gtaaactaga 120 ttattttgta aaccttttct acgtcttttg gggtgtcagt tgttaagtgc tgagcttctt 180 tctattccaa atctatcttg cgctcctgaa aaactgcagt aaaggcactt gaaagctgtt 240 ttcctaagat acgatttttt tttccttctt gctggtactg cactgttgca ccaagtgtgt 300 gcaattttta ttcaaggtca tcgtgatgct gagaagtctc attgatcacc tgtccatctc 360 tggtctcaac cgtcttaatc aggagtgttc tttttgagtg ggtgtcaacc agaggaagtg 420 actccaggtt agtttctctc aggttcaggg aagaaaaggt tggcagaggc agagaaatcc 480 tgctctcmnc gccttccagc agcttcctgt aaggnggcga ncgtcaatgt ccagggccad 540 cttaacattg agccagatct tggaattcac gmaggtga 578 205 530 DNA Murine misc_feature (1)...(530) n = A,T,C or G 205 gaattccgac ttcaccatcc ctatcaaaat actgtcaact tctaaccaca atagtgactc 60 tgtgcttgtc tgtttagttc tgtgtgtaaa tgaaatgtgg aaatgaccct ccctgcccca 120 gctggctgcc ctcccctttc ctttgatctt gaccactcat ggaagcagga ccagtaaggg 180 accttcaatt taaaacaaaa caaaacaaaa aaacaataaa aaggctaatt aacaacaaaa 240 aaaaaaaaaa aaaaaaaaaa aaaaaaaggg ccghgaattc caccacactg gcggccgctc 300 gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc 360 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 420 gcacatcccc ctthbgccag ctggcgtaat agcgaagatg gcccncaccg atctgccctt 480 cccaacagtt gccgtcatcg ctgaatggcg aatggrcgct sccctgtagc 530 206 501 DNA Murine misc_feature (1)...(501) n = A,T,C or G 206 ggcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc 60 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca 120 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc 180 ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt 240 ccagggcggc acggatcact gtattcggct gcaactttgt catgcttgac actttatcac 300 tgataaacat aatatgtcca ccaacttatc agtgataaag aatccgcgcc agcacactgg 360 cggccgctcg agcatgcatc tagagggccc aatncgccct atagtgagtc gtattacaat 420 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaaccttaa 480 kcgccttgca gcacatcccc c 501 207 561 DNA Murine 207 gaattccaat ctcagaataa aggatgacca ctggactctc aggatttgat gagggatatc 60 tgtgatctcc tttgaacaat aatggtttcg gtctgtcagc ggcagtcagc agaaggctct 120 ccagagtgtc tagatcacaa gtctgctttc catgcactga gagaaacgac ttgcaccctt 180 ctggtggagg ctcgtcaact gctatctgct ggaaggcttg aattgaggct gagtaggaac 240 ggagagagag acaaaacttc aacaaattct gctgcagagg ggacaggaag cgaaacgcag 300 cttccaatac ggcatcgtaa taggagtgat cagtatcgtg atgatctgat gatccaatgt 360 tttgagtggc ttctacaaaa ctccaaaatt tctcttgact gtcttctgct aagaactcac 420 tggcttccag cagcagtggg gcagaaaacc actttgtggt gagagaggtg staatggctt 480 ttgaattggc ttctgctaag gaaaacaggc acggtaaggc cagtgcaatc waggagatct 540 crtgtatgta acggagmcct g 561 208 547 DNA Murine 208 gaattcgcct gggaatgtcc tggggaagaa gagcagagtg tttctgcccc ttggcccagg 60 cagtgcagac aggaagaatg catggggtaa gggtaggcca gtaactccac ttgcaaagga 120 tgtagcactc actggctagg atgcatgggg agagagttac tgctgccagc tttcctctgg 180 tacccgctat agactggcat ccagagatgg gtgcctggct tgaggcctga gacagtgatg 240 cccttctgct ggtggccaat gctcctgtta agctgcttac tgcaaggctc catcttctgc 300 atctgtgtcc tggctgtgct ccagctcctc ctcgctatgt gttagcagtc cctcctcatc 360 accatcatct cgagtttgga cttctccttg gggtgtgcct gcctcagaag ccgtgtcttc 420 ttggggcgct ggtagccggc tgctgctgct gcagctcccg ctgccgccgc cgctgccacc 480 accaacattg ctactgccgc ctccaccact gctgcctcct cctccacact gbgctsktca 540 cccttyt 547 209 644 DNA Murine 209 ggaattcttt ttttttatat gtaaaacgac aaaatatttt aattttccat gaccacaggc 60 tctcttcaag aaggctgtac ctgtatgacc accaggtgac agcatggata atgcttcagg 120 acaagtcaca attttgtact aacaatcagt tcaaccacag cttgaaatgt agtttgtccc 180 agctgcaaaa gccacaagac accaatcatg cgtcttaccc cagtacagac ttttataaaa 240 cacacatgta tgtaattagc acaataaacg cgcttattat gcactctaac atagagcaca 300 ggaatacacg ctatggagtg cagccctcat gtctccacag gcaagagcta gagggttaaa 360 caggagccca tggtgtgaca gcaggagctc ggagcgcacc actctgcacg tgacttaccc 420 tacactgaga actgtcaccc tgtccagtgg gtggcaggta cagtctcata aacagtgtta 480 tttcctagag cagagatgtc agtctggatg tgagtcgctg ttacctagaa ggsattacaa 540 gtcagctcca tagaaggtgg gcgtttggct ttggggtcga gtgtaacagt gtcccgcaga 600 cacttkcaca cccgcacccc tgtgccccag gggagtgcmc ttcc 644 210 442 DNA Murine misc_feature (1)...(442) n = A,T,C or G 210 tggaattccc agtgtcacgg cactgctgct tacagggccc gccacctcga cagcggtcat 60 tcaggtacgg gtcttcttgg tcctcctcgt caggaatctt agctgggtcc tgaaggtctg 120 caccgttgcc ttggacaaag tctgaattct cccgggcctt cacacagcag gcacggaaca 180 ccagcccaca ctggtagctt atcatgacaa tgggttcaca ggtctggtct cgggccaggg 240 atgcctttcc cagcatgcaa cagtggcagc acctctttat gaagatggtc tcaaggctac 300 tgttgtagct gtggagcgag gcncagcttt cttggctcgc tkggccargg ttgatgcccg 360 tkgcacagtg gcagctcttt ccagtttggt tgtgacaaca tttkctcatk ggrccattct 420 gcacdccytt ggattctbga gg 442 211 496 DNA Murine misc_feature (1)...(496) n = A,T,C or G 211 ggaattcccg tccagctccc cgggcggtgt ggagaagcgc aagctcccgt tctccgagga 60 gtgctctgat gaggaggcaa aaggcgattg tctggagtct ccgaaagtaa ggaagggatc 120 tttgagctgc ctggaggccg catagccagc gagccactgc gaatacacgt tctccgtgtt 180 aggcatcgcg gccgggggca ggtcaaactc cttctccagc ttgatgcgct tggagaaggg 240 gctcagcgag ctggggctac ccagcagcag ctttttggac agaccccccg aagccgattc 300 gccgggggag cagccacgac cattaacagt gccatcgtct atgcggtctg actcaccggc 360 caccgagtct tyatcacaag tgttcccyaw ggscctcsgg ctctggccag gtggctacsc 420 ttatgctttt nncccaggac cttgtggaag gcctctctba agtgctgcat ggagctgagc 480 accatgccct gcatga 496 212 430 DNA Murine misc_feature (1)...(430) n = A,T,C or G 212 ggaattcccg ttctcctgta taggaggcag ccatggcgcc cagccggaat ggcatgatac 60 tgaagcccca cttccacaag gattggcagc agcgagtgga cacttggttc aaccagccgg 120 cgcgcaagat ccgcaggcgc aaggcccggc tggcgaaagc gcgtcgcatc gcccctcgcc 180 ccgcgtccgg ccccatcagg cccatcgtga ggtgccctac agtgagatac cacaccaagg 240 tccgggctgg caggggcttc agcctggagg agctcagggt ggctggcatc cacaagaaag 300 tggctcgcac catcggcatc tctgtggacc cgaggaggcg aaacaagttc acggagtcac 360 tgcaggccaa cgtgcagcgc ctkwaggagt wyckctccaa gctcatncct gttccccagg 420 aagccytytt 430 213 383 DNA Murine 213 gaattcgctt gttctgtcat tttctttcct tggtaaactc tctggggatt ggtctgtwct 60 cagctgtgac tatagtcaca tcctggttcc cagcagaaat kgtgaaacaa cctgcwgcct 120 agcccacagt actacagttc tctgttttgt ttctgtttct agcccgtctc gatactgaca 180 actggagttg aagctgcttg aagtaagtct gatgctttca tataagtgaa tttgtaggac 240 tattgctttt wrtttttaca acagaagtaa ttctgacata ttaagtggaa aatctaaata 300 agtatataga ttatataaca tgattttaat tacatkggat ccaactacat atgtgattag 360 ataatgtgta tatgtacata tgt 383 214 166 DNA Murine misc_feature (1)...(166) n = A,T,C or G 214 gaattcgaaa tccctatgct gdnmagagga aagccagcta agttttnwrc tgtgtttwrt 60 tctaaacgtg atggtgtytc tgaggccaaa aagtacaagg caagtttwnc aatatttctc 120 tgcaaagaag caaagagaga aataagaccm sccagcaatt gaattt 166 215 231 DNA Murine misc_feature (1)...(231) n = A,T,C or G 215 gaattcctcc gattcattta ttaggacatg atctctgatg aatctttact tcccaattgc 60 taggcttact agcagcaagc acacctgcac gagstccaac atgggktctg gagatcctac 120 acaggctaac aatttdcnnn vcttctaaaa tggaattctc acaccaaacc acttacctct 180 tctttgrttt tctgbacaaa gtcaagtcaa cataggacag ggcgtcgctc t 231 216 294 DNA Murine misc_feature (1)...(294) n = A,T,C or G 216 ggaattcaag agaaggaaag agagaggggg agagaaadaa agaaagaaag aaagaaagaa 60 agaaagaaag aaagaaagaa aaagagagag agagagagag agagagagaa ataaagaaaa 120 rgctaaammt ddmwrvwrct taarmtctta tagaaccaca catcattttt gtttgactta 180 tatcccmtct bgcaatmtca aagtccagtc caacaagagt tccmgcttcg gacacacatt 240 tggtcaggat gatggtggtt artawctvnm tgtgntctgt ctagrwcmaa actc 294 217 506 DNA Murine misc_feature (1)...(506) n = A,T,C or G 217 ggaattcctc cagggtagtc tggaggtggt gataccatag gagaatccaa gtttacaatg 60 gatttcataa caatttctaa agcatttctt ccatactgtt taaaaaaaaa aaaaaaaaaa 120 aagatgtttt aaccaggctc accatttggg taattttttt gaccaattaa atgctataaa 180 ttataattgt accaaatatt cagaaactat tatttataaa tattcaggac attaattacg 240 accgcctatt tgtgcctttt cagacagcag acattcaata tgttaatact tttttaattt 300 ttaataactc atcttgatgt tttcccaaaa ntnccaggag tattttccaa aaggaataaa 360 aaaaatgtat gtatagatca tgatatgtca aatcctgtct cacatgaaaa taccagaagg 420 caaagctaac aagagcaagc aagtagagtg gttagnnhca catcactaga gacacagaaa 480 tgtaccttgt tgtcaaagtt gaatct 506 218 492 DNA Murine misc_feature (1)...(492) n = A,T,C or G 218 ggaattccag aggaagggag ctcagaagat ggaacgaagg ctgatgagaa gagctctgac 60 caaggggtgc agaaggtggg agatactgat ggcactggta atcttgatgg aaagaaagaa 120 gatgaagacc ctcaggatgg agggtccctt ncctcaacac tgtccaagtt gaaaaggatg 180 aaacgggaag aaggaacagg ggctacagag ccagaatatt accactacat ccccccagca 240 cactgcaagg tcaaacctaa tttccccttc ttactcttta tgagagccag tgaacagatg 300 gaaggggatc atagtgcaca ctcaaagagt gcccccgaga acagaaaaag cagctctccc 360 aagccgcaag ctgttagtaa gacagcagca agcccagggg cagaaagaac agtgagtgaa 420 gcttctgagc tgcaaaagga agccgctgtg gctggncctt cagagcctgg nggcaaatgc 480 atgaaacmaa ga 492 219 458 DNA Murine misc_feature (1)...(458) n = A,T,C or G 219 ggaattctaa tcatatgtca gagaaatagt aacttcacca taagtgatag tgaaatgagg 60 aactgtgagc tataaagaag ttatgttaat gtgtgagatg tcttttcaaa aataaagttg 120 tactatggac aaatactatg tgaaacttat ttattgtaat tttttctagt atttataatt 180 attttataca acttttatgt gtttttgctt ttcacttgac aactaggcaa taatcttgca 240 actttcttcc aggtcactta gatatgttca gtacattacg ttcctctagc ttgtacaggc 300 aacatccaaa aactcttcga agcatttgtt cagatcttca gtattttcca ggtacaaaca 360 agtgtattat ttattttgra aaacatagtt atatttagta agacttgttg tnmscmgddg 420 gtggtaattg aagtacctta ttccytggta tattaagt 458 220 319 DNA Murine misc_feature (1)...(319) n = A,T,C or G 220 ggaattcatt caaacactga aaaccaaatt ttataaacaa ccatcaaatc tatgcagttt 60 gcagattttc ctcccctcct tgaaataatt tcagaagcat acacagaggg gtccctacac 120 taagaaggca ccagggcccc agtttattcc agtttatggc cttttcctgt gtccgagggc 180 agccttatca gcaggcatag actggtcaaa ctagccccgg aaagmctgct ttatgaactt 240 caatgacgat yccatcctca aaaangccta atcaacyacc gtctccattc ttttccmaca 300 ctgactagtt aaacttatt 319 221 221 DNA Murine 221 ggaattccag gctcgagcgg ccgtatacta ttatatwaat caaaacattt atcctactaa 60 aagtattgga gaaagaaatt cgtacatcta wggagctata gaactagtta ccgcaaggga 120 aagatgaaag actaattwaa agtaagaaca agcaaagatt aaaccttgta cttttgcata 180 awgracttaa cthagaaaac cttcttaact aaargaatta c 221 222 285 DNA Murine 222 gaatthggca taaatcaaag ggggtgaaat taaagcaatc ctttctgtta tttctcacaa 60 gtggcagatc tgtattttgt ttatagaaga ctgtagatcc ttttaaatga cagacagaat 120 tcttaarrra ttttaaggca tggagaggta aatgacaggt ttgtacatgg agtaaataag 180 gtatcaaaag tagaaatatt aaattatggg agtggagaga gagagagaga gagagagaga 240 gagagagagg agagatcgac agagagaata caacgtttgg ttagt 285 223 473 DNA Murine misc_feature (1)...(473) n = A,T,C or G 223 ggaattcgtg acctcactgc ttagttcctg gaaagcttgg gacagacagg ggccttggct 60 agactgtccc caacacccac tccctgccat gctcagtgtt gggcttgggt ttcaccactg 120 gggcagcaag gcaggccagc ggggcctctc tgggctctgg aaacaagctc tgccacatag 180 ctctgggcac agtccatccc ctggggcctg aaggaggtca ccgggaggtg atctttttcc 240 acctctgatt gagcaagaca aggccactgg ggacaactga acagcacagc caaactttga 300 aacagagaga cagggccagg caaagtgccc accctgcccc cactcttyct gcgttcbabn 360 ccagtctccc tgggggagtc agtgacggga tctgggggat gttcctctcc agatctgttm 420 actggccttt tagaaatgcc tcctggggat tgtgaattag tagagcagtt tgt 473 224 342 DNA Murine 224 ggaattcata agaatgacca aataaaattt tgggagcaat aaatgtagga gaaaaatctt 60 tggtgggggg tttgggaaag cttaactttt taaaggataa tgtcttttta aaaagaacat 120 ctctggctct gactgttgaa aatacttaag atatacatac cagttttatt tgccttaaaa 180 tcaaacagag aagcaatgct ttaacagata aaaacagaag gtcaaactag ggctagagcc 240 tgttagggaa agragaaaag gctaacctag kggactcagt ggtgttaact gaagatagct 300 accacatgca agatgtwcac gggcagagag tttatcctga aa 342 225 89 DNA Murine 225 gaattcgcgc gctgtsttcc cgctcgcgtc agggacctgc ccgactcagc ggccgccatg 60 gcatcagatg aaggcaagct tttkgtggg 89 226 283 DNA Murine 226 ggaattctct ccattactta cttgtctctt cttagtgagt ggtaaccgwt gagtctctaa 60 gagstctggg gtcatctcag gagtgctatg ctcagcttat gcattatggc acccggcagg 120 ggtcattttg ggcatggtct gctccccaga tcagtgtgag caccagactg gtgatcatct 180 caggctccct ccctcttggg agccccatag cacctggtgg ttgtctcarg gtcttctgtc 240 ttggahtchm tyccacacag cctgtggtcc taggcaggat tcc 283 227 259 DNA Murine misc_feature (1)...(259) n = A,T,C or G 227 ggaattcggg aatccttacc atcacacaaa acttacatca gtgctgtgaa atgtaacaga 60 aaatctgggg atgcctgact ttkgttattt ccctggtatt ttattaagct tgagtatggt 120 taatatttat gctggcgttg cattaatctc aaaagattag cacctatatt ccatggattc 180 tctcghgctt tagtccaaat atttttaacc ngggcatggc agtacaccac ctttaahccc 240 agcacctgag ggaggcaga 259 228 390 DNA Murine misc_feature (1)...(390) n = A,T,C or G 228 ggaattccca gactgaggaa gacccggaaa ctccggggcc acgtgagcca cggccacngc 60 cgcatcggta agcaccgcaa gcacccaggc ngccgcggga atgctggagg catgcaccac 120 cacaggatca actttgacaa atatcaccca ggttactttg ggaaagttgg tatgcngcat 180 taccacttga agaggaacca gagcttctgc ccaacagtca acctggataa actgtggaca 240 ttggtcagcg agcagacacg ggtcaatgcg gcaaaaaaca agactggngt nnmtcccatc 300 attgatgttg ttcgatcagg ctactacaaa gttctgggca aggraaavvt ccctaaagca 360 acctgtcatc gtgaagccaa attcttcagc 390 229 415 DNA Murine 229 ggaattcgga gaacttcact tcaatcagct tccgagggtt tagggatcga tgccagtacc 60 tgcaggtgcc cacaggcttt ggcaacacca ctccggcagt gtaaacagct tggaaaatgc 120 cctccaggtg gacccgccgg gtgatctctc ggatcaaaac tggagccacc ctcttagagc 180 gcagcttctt gtggacacac aggaagttga tctccaccat cttcttctct gtgtcataga 240 tgtggatgtt tgctgggatg gcactgatga acccaaccag tttccgactt gagaccactc 300 ggaccccaca gtgccactgt gggagccaac ctggtkgccb gagagcccac aagagaract 360 tctdgggraa tagtcgaatc ggaacatatk gtcatcatct tccacggtag tttct 415 230 273 DNA Murine 230 ggaattcttt tctattaacg atttcaatct tcatgaagac aaagggacaa taagagatgt 60 catgacccca acacttaggg taagcaattt ttgtkgcatt tgttattagc tgttcttgaa 120 ttagcttatt caaattttct tacaggagcc aaaaaggagg gagagacacc caatttgawt 180 attttaaaat ttaaacaaag aagtaaacaa accygttaaa akgtttcaca tagcacagtt 240 tggggaggga gaacaaatca ttttctgvcc ttc 273 231 230 DNA Murine misc_feature (1)...(230) n = A,T,C or G 231 ggaattcccc ggctcgagcn ngccgctttt tttttttttt ttaaagcaaa atcttggaat 60 attcttccca tatcatatat tttattagac aatattatga tttttgtctg gtctttaata 120 cccaaaggga tggctgtcca ctaactcaaa accaccagkt ccttcactac ctacaacagt 180 ttagratcag ktttaaaacc cctttctcat caagrggcag gacaatttaa 230 232 359 DNA Murine 232 ggaattcttt tttttttttt ttttaaattc agacaaccaa gttcattgga agtgtatgta 60 aaatagaagg taaccttcct gcaggagaac caaggggctc tcctgtgagg tagtgccacg 120 ttatgaaaac tatgaaaact gaaaagtatc ctcccttttg caaaggttct aagctgtgtt 180 acagatactt acaagaggtt taagatgtga gtgaacgtgt ccctattgtg ttctcattta 240 tagccttttc tatgaactgg tgatgttttg aagtatgagt ttatgaagtc tctttgtgaa 300 cctggacttt tatttctaaa gtttgaacyk gtgtgacact agagkttacc tgaatacaa 359 233 362 DNA Murine misc_feature (1)...(362) n = A,T,C or G 233 ggaattcccc gaattgtaaa taacttcata ttgggatctg cattaggtgg agggcttctc 60 tgcagttcta ttcttgcacc agactgttgg cttatgcttt ttatggtttc acctcctttt 120 tycaatgatc agtccagttt tcccagttgg cacaatgaaa ttaaactcct ggngtccacc 180 cgggggcccc atattccagt ttccttgacc tctacctcgt cctcgaccac caggtcccgg 240 tccaccagga ttgccagcct gaacacttcg tagaaggtct gtgattattt ctgcagcgtg 300 ctgacacctg tytggaggtc ctgtttatct gtgccatwcc tawtcaggtg ttgttccatc 360 at 362 234 217 DNA Murine 234 gcggttaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc 60 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca 120 gtgcgtcgag cmgcscccgc ttgttcctga agtgccagta aagcsccggc bgctgaaccc 180 ccaaccgttc vccagtttgc stgtsgtcag accgtct 217 235 325 DNA Murine 235 gaatccgcgg ggaccagccc ggcagaatgg ctcccgcaaa gaagggtggc gagaagaaga 60 agggccgtct gccatcaacg aggtggtgac ccgagaatac accatcaaca ttcacaagcg 120 catccatgga gtgggcttca agaagcgtgc tcctcgggca ctcaaagaaa ttcggaagtt 180 tgccatgaag gaaatgggga caccagatgt rcgcattgac accaggctca ataaagccgt 240 ctgggccaag ggaataagga acgttccata tcgcatccga gtacvcttgt ccagaaaacy 300 gtaatgagga tgaggatccc caaac 325 236 521 DNA Murine 236 ggaattcggc cctttttttt tttttttttt tttttttttt tttttttttt tttttttttt 60 tttttccatt ttagtggaca tctttattgt ttaatagatc atcaatttct gcagacttac 120 agctgggatt tcatcagatt gccatgctga gtcaagaaca gtgagtgacg aagctaacca 180 gaggctacat acgtcagaga gagagctcag cctttacagc tcacttcctt tctcaggcag 240 aatataaata gacgccctct acaatgcaca atggttttag tcactaagga atttaaatgg 300 gatcttgaag aacacagaca aatcctgatg cagtaaagac gagctgagat gctgtgcaac 360 tgtttaaggg ttcctggtgc cacatctcag ccactagctg aatcttgcgc taacaccaaa 420 tggagawgtg gaaaacacta ggttgactta ggagcacagg aaccaaaggc gggaaagaaa 480 atactaaaca ttgctgagag catccacccc aggaaggact t 521 237 301 DNA Murine 237 gaattcgcta tgagaaggtg gcgagactgc agaaggtgga gacagaaatc caacgggtct 60 cagaggctta tgagaacttg gtgaagtcat cttccaaaag agaggctctg gagaaagcca 120 tgaggaacaa gctggagggc gagattagaa ggatgcatga cttcaacaga gatctgagag 180 accgtctaga gactgccaac aagcagctgg cagagaagga gtrcgaggrr tccgaggaca 240 ccaggaagac catctsgsag ctctttgcca aacataaaga aarccagcgg gagaaggaga 300 a 301 238 483 DNA Murine 238 gaattcaaac accactacaa aagacactct atcaaaatca gagtaagaaa aatatgaaaa 60 ctttcttgct ttctgattat cttacgtgga accggaagga aaagctagtg agaggatatc 120 aagtcacttc taacaaccac agagttataa acctatctgg tgttgaaaat caacatgaaa 180 acgaaccagt cactttgact aaatataagg ctgtttgtta catgccttaa ggaaccactg 240 ccatgttcaa catgtggcaa aaagacaggg catgtttgga attcatcttt aaaacatcct 300 gtctgaatgt accttactcc gaactaagtc acattttcta gaggtcccat gagaagaaag 360 twaaggatat cggtacatta ctctaacaaa aacttcagtt aagcattacc gtggctgttc 420 actgctaata actagagrgg catgttaagc tagggaagct aaggtcagca cgacgtctgt 480 aaa 483 239 469 DNA Murine 239 gaattcaagg ttttggatac caaaaactac aagcagactt ccgtgtagat atgttgatga 60 agatcctgac tctctaggat tgtactttgt gcttcaacta ttcaaggcat agcatgaatg 120 gacgtccatc ttacaaaata acctgtgtga agatgaatga ttcggcctga agcagggaag 180 ttgatcagta ttgatttgtc tgctctcaca aagttctgaa cagcaatgat acgcccagtt 240 ttctgcctta agtggttgtt ttccttgtga gcattgtact gaactagatt aagaggacaa 300 aattaatgaa taaggtgttc chtgaacttc tgtacgcact gtctactcaa cattatccat 360 atgattctta cctgatccat gcatttattt atagttacta acaaatgtga aawtactgat 420 cctttgctct gaacttgaca tccagahcyc agatttctca tttattcac 469 240 200 DNA Murine 240 gctggcgcgg attctttaht cactgataag ttggctggac aatattatgt ttatcagtga 60 taaagtgtca agcatgacaa agttgcagcc gaatacagtg atccgtgccg ccctggacct 120 gttgaacgag gtcggcgtag acggtctgac gacacgcaaa ctggcggaac ggttgggggg 180 ttcagcagcc ggccgccttt 200 241 477 DNA Murine 241 ggaattcggc aaacgctcaa ctactgagct acagtctgag ctcagtataa tttttaagga 60 ttttaccaat gcttaaatgc tgttgcttga tgttactact tatcctggta tagatggtga 120 aaattttcag atatgtggat ttttatcatt aacatggaaa aagaaaatta gttttaaaaa 180 gttatggatg tgtctgtgta gcaggtgcat gcattgccta tggagthcag atgtgggtat 240 caaagtctct gtaagtggag ttacagattg ttgtgaactg tcatgagaat acttggaact 300 gacactgggc cctgggaaga gcaagcagta ctcttcactg ctgagccatt tctccagaca 360 gcaacatcct aaacmggtat tctggaatcc cacaccccta gtcatatttt cagttaggct 420 aaaagattca ctcatacttt ctcctcttat acaggaatct gtgtatctct gtacaga 477 242 535 DNA Murine 242 ggaattcatc ctttcaaatt ataatcattc tgatagaggt attttaatat acatgctttt 60 aaaaacaaaa caaaaaacta ctgtcagtat gaatactgag ccagactggc atatatagat 120 ttaacatctt gtcctactaa gattcttaac tgtataaaaa taatatggct tttagacata 180 taggatacta atttcaatga gacccttatc tctttattga acattatgtt agggacagta 240 aaagccatgc acttacctgc tacccattgg aaaataaaac gactgtcccc aacctaagta 300 agtatgaaaa ttaggctagc cttatttcat ctttaactac taaaagtaag tctatagaac 360 ttaaaattta agcactatta gttgtcatgg ctatatttta ttttccaaaa attaagttaa 420 aagtcattaa tgtcattgat tatatacatg tatgtttttc taataattaa aatacctttc 480 aaatccatgg aatgtctggc ttttaaatgt aatttgacct ttycgccytg atttt 535 243 364 DNA Murine 243 ggaattcttc tggtcatggg caacattatc aactggtcgc tggctgcata cggactcatc 60 atgcgcccca atgactttgc ttcctacttg ctggcaattg gcatctgcaa cctgctgctt 120 tatttcgcct tctacatcat catgaagctc cggagcgcga gaggatcaag ctcatccctc 180 tgctctgcat cgtctgcacc tccgtggtct ggggcttcgc gctcttcttc ttcttccagg 240 gactgagcac gtggcagaaa acccccgcag agtccaggga gcacaaccgc gactgcatty 300 ctyctcgact tctttgatga ccacgatatc tggcacttcc tgtcctccat tgccatgttt 360 gggt 364 244 600 DNA Murine 244 ggaattccac acatgcactt actcatgcat gcatgcacaa acacattact actgatacag 60 atgtcagtat tcccagaaag agagttcaaa agatattatg actgtattcc acgtattcaa 120 aaatatcagt tgaataagac taaaattaag cttatagcaa aaaactacac atagtgtaac 180 aggaagaata caagaagttg acagcaggct atactatgtc acaggttggt gaccatggag 240 acagtgactg ctcagcagta ggaagtgtgc tgagtgaatc actgagacaa acttcttttt 300 aatgggcaga acatccgtga acttccttta accaaataat atatagttgg aaaagtcaaa 360 gaaaaaagaa tacctagaaa agtaatatct gaaaaatttc caaattttgt acaaaccatg 420 aatccatata ttcaagcaca agaatcaaag aaagaattac atttaagatt ctaaaagatg 480 attagaaaga gaaaattata aatagttatg tgttatttaa aaaaaaaaat ctatgacgac 540 taaggctggt ggtatatacc ttcactcctt gaactcagga agccbaggca ggtarggtgt 600 245 325 DNA Murine 245 ggcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt 60 gtcaagcatg acaaagttgc agccgaatac agtgatccgt gcgccctgga cctgttgaac 120 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggv ggttcagcag 180 ccggccttta ctggccttca ggaacaagcg gcctgctcga cgcactggcc gaagccatgc 240 tggcggagaa tcatacgcat tcvgtgccga gagccgacga cgactdgcgc tcatttctga 300 wcgggaatcc cgcacyttca ggcag 325 246 239 DNA Murine 246 ggaattcgta agaacaagca aagattaaac cttgtacctt ttgcataatg aactaactag 60 aaaacttcta actaaaagaa ttacagctag aaamcccgaa rmcaaacdag ctacctaaaa 120 acaattttat gaatcaactc gtctatgtgg caaaatagtg agaagatttt taggtagagg 180 tgaaaarcct aacagcttgg tgatagctgg ttacccaacm tgaatttaar ttcaatttt 239 247 377 DNA Murine 247 ggaattcgtc ttgtctggac aaaaatggtt ggtttaaaag gccaaagaaa gtgctggtag 60 aaatgagagt actaattagc ctccaaaaag agactgttct cattgtcttt gtacctcagc 120 catagcctgg tgcactgggc acatggtcag tgtctcagaa aatgtttgtt gaatgaatgt 180 tgtttgtttg tttgtttgtt tgtttgaatt ctggaaatta tttgttgaac acaaagacac 240 ccagcaccta ctgggtgctc actgttgtga gagactaggg ctgghhvctg ggcagtaggg 300 acagcctcat tggctaatta aggatttttt tgcaattccv ggcgatttac aaggcacttt 360 cttgtgagtt atgtagt 377 248 452 DNA Murine 248 ggaattcccc taatctccat taacgaaaat gacccagacc tcataaaccc aatcaaacbc 60 ctagcattcg gaagcatctt tgcaggattt gtcatctcat ataatattcc accaaccagc 120 attccagtcc tcacaatacc atgattttta aaaaccacag ccctaattat ttcagtatta 180 ggattcctaa tcgcactaga actaaacaac ctaaccataa aactatcaat aaataaagca 240 aatccatatt catccttctc aactttactg gggtttttcc catctattat tcaccgcatt 300 acacccataa aatctctcaa cctaagccta aaaacatccc taactctcct agacttgatc 360 tggttagaaa aaaccatccc aaaatcacct cawctcyttc acacaaacat waaccacttt 420 aacaaccaac caaaaaggct taattaaatt gt 452 249 499 DNA Murine 249 ggaattcgaa aaaacaaaaa aattctgcat gctcagatgc acagactaag actgggtaac 60 ataagccatg caattgccaa cgtgctacca taatatatag tatagtgagt attgtcatca 120 catgacagta ttcagtgcaa tagttatgta agatttactg aattgtaaag aattggaatg 180 catataggat atatttgatc agttttctta catttagcat atttatatta cccatcttat 240 ttgtgttatc tctaatgttt cattatggct cgagccttat aaattaatgt cactcacaaa 300 ttcttattag ggaaaatagc cgtatgctac ctgctaatac ttaccaaatt agtatcttac 360 ttcaaaagat gttttgctaa aattttaata aggaaatagc atgctatatt ttctaatttt 420 aattatatgt gaacaagtca acataattta tatgarttta aatctccaga tacttcagaa 480 attggtgctt gtacacgtc 499 250 399 DNA Murine 250 ggaattcagc agagcacact cccaagtgca cagatttaac acagtagcga ctatttgcat 60 ttacaggact tttcaacaat ctgaaaaaag atcaactgtt gaagatctgt aggtatgtta 120 caaaaaccac tggagttctt gtacaacagt atgcgttctc agcaaaacca acaccaggag 180 atccgcatgg caactgagta accgatccac tcccgccaac ccaggggcag gtctccgtga 240 gctctaagct gtcttataca aaagttaagg caaagtcatt ttcaagttta aataaaattc 300 aagtctttaa atatttggat ggaaataatt tttttycctt agaaaaaaaa aaagrraaaa 360 gaaaccaaaa caaccttcag tctcattaaa wagcatttt 399 251 183 DNA Murine 251 ggaattcgtt ttatcttaaa atcatatgtt taaggcagta agacactaaa ccaaaacaaa 60 aaacaaaaaa cagggacatt ttaacaactc aactcccatt gttctctgtg gcatttattc 120 agcaagcaca tggaaatagc aaamgagaat ctacaatagc tgtcccaaat gcaattacac 180 atg 183 252 396 DNA Murine 252 gaattcgttt tatcttaaaa tcatatgttt aaggcagtaa gacactaaac caaaacaaaa 60 aacaaaaaac arrracattt taacaactca actcccattg ttctctgtgg catttattcc 120 agcragcaca ggaaatagca aagagaatct acaatgctgt cccaaagcaa ttacacrtgg 180 aaagwttacc aatgcagggc tgggstttga aagccaaagt gttagtgmag awacagagct 240 tgacacctag caagragara cgagtttgga gcsttggtgc tcaagtmttg aaagattgaa 300 mtmtttgaag tmgttcatta gtcatcaaag gtcactatgm aatagttgcr actttaggtg 360 taaatctgtg tggggagttt ttatagcctt tggcag 396 253 407 DNA Murine 253 ggaattcccc ccttttacca gtggatggac acagagaact tcgtgttgcc tgatgacgat 60 cgccgtggca tccagcaact ttatggaagc aagtcagggt cacccacaaa gatgccccct 120 caacccagaa ctacctctcg gccctctgtc ccagataagc ccaaaaaccc cgcctatggg 180 cccaacatct gtgacgggaa ctttgacacc gtggccatgc tccgaggaga gatgtttgtc 240 ttcaaggagc gatggttctg gcgggtgagg aataaccaag tgatggatgg atacccaatg 300 cccattggcc aattctggag gggcctcctg catccatcaa tactgcctac gaaaggaagv 360 mhcaaatttg tcttcttcaa aggagataas actgggtgtt tgacgaa 407 254 354 DNA Murine 254 ggaattcccg gctcgagcgg ccgctttttt tttttttttt ttttttttaa tcattaaggt 60 aattttatta atatagatat ctgcagatca agtgaatggt actaatgaat agttttggtg 120 acctcaccct ctcatgtata acactgaaga ttcttccact ccatgttcac tccagactct 180 cagttttaaa gcaagcatca cagaatacca ggctcttaca gtgatcggga gcyagagctc 240 ttacacaaag ccatactcca cmhgctgaca gtttctttag taatacatat agtactatca 300 gataactcat tccaacaaca aaaaattahh cattatgtca accaattgcb ccat 354 255 575 DNA Murine 255 ggaattcagc agagcacact cccaagtgca cagatttaac acagtagcga ctatttgcat 60 ttacaggact tttcaacaat ctgaaaaaag atcaactgtt gaagatctgt aggtatgtta 120 caaaaaccac tggagttctt gtacaacagt atgcgttctc agcaaaacca acaccaggag 180 atccgcatgg caactgagta accgatccac tcccgccaac ccaggggcag gtctccgtga 240 gctctaagct gtcttataca aaagttaagg caaagtcatt ttcaagttta aataaaattc 300 aagtctttaa atattggatg gaaataattt ttttccttag aaaaaaaaaa agaaaaaaga 360 aaccaaaaca accttcagtc tcattaaata gcattttgtg gaataagctg tatggttaca 420 tatagcagga aatagtttaa tgtctgctgc ttagaatact taaagaaaaa tcttaggcgt 480 tttaaaacaa aataatttat ctgtaacttt attatgaact tgctaacttg actgcactct 540 cgctcctcag aagtgccgct tctgacaatc tagga 575 256 588 DNA Murine 256 ggaattcccg gctcgagcgc cgcttttttt ttttttttta aatgccatag cagtagtagt 60 tgggtctggt ggtggcacac acttttaatt ccagcgcttg aaaggcagag acaggaggat 120 ctcttgagtt taaggctagt ctggtctata ggcctgcaag gacttgaggg gaaataaaag 180 gtcactacaa gccatttctt attttaacca atagcattaa attgtgccta tagtgattct 240 tagttgagac attgttcaga atgacttcat tctgtatgct tttgcctatg tctgtgttgt 300 atgcattaaa tattttgagt gacaatcttt tagtaattat attttttcca cagaataata 360 aaatatagga atcttaagca gtgtatgtaa caatattttc cttgacgtag acagcacata 420 cttttaaaat acaacttagg caagcaaaca cttttgtact taataattta atgaatagaa 480 gttagttttg tttttagtct taagggtgaa aaggtaactc aggctttaaa gcaagacmgc 540 accaagtgcg agctgtgatg tsccagcagt gtaactcttc cccacccc 588 257 205 DNA Murine 257 ggcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt 60 gtcaagcatg acaaagttgc agccgaatac agtgatccgt gcccctggac ctgttgaacg 120 aggtcggcgt agacggtctg acgacacgca aactggcgga acggttgggg gttcagcagc 180 cggcgcttta ctggccttca ggaac 205 258 249 DNA Murine 258 ggaattcgtc gagcggcgct tttttttttt tttttttttt ttttaacata agcaggcatg 60 gtggctcagg cctgtaatcc cagaatgtgg ggctgcaata gcatgtcact gtgactttvv 120 vcccatttca aaaatccact taaaccatcc ccaaaacgag tgtgagagag gattacagat 180 aactaagtaa aaaatgtcag tggtcaccgt tatctattcc tgggtcagaa gcggcatgtc 240 catgaaggc 249 259 389 DNA Murine 259 ggaattccaa cggttgaaaa cttctggatt agagatttag agctgtgctt ctggcaactg 60 tgttcttcca tggtggactt ccagctaaac agcactgatt cttgtccctg tcatgtcaga 120 tactgcaggg tactcactca ccacagtaaa gtcatgcttt caaaaccact cacagctact 180 caaaggcaac ggcaaacaag ccccaaacat ctcatggcta tattaacctg gaattctgtc 240 acgtcaggag cattcttata gacaaaacaa tgtaaaactt aggatttaac aacacagtac 300 tggtgtcacg cccagaatct tacccatcat cccagaagag accagcacca agggtcagag 360 gatggaattt kccatacaag atgagggac 389 260 228 DNA Murine 260 ggaattcccg atgctgcttg gaagccttgg ctgaaacvct accacagcca gacctacggc 60 aacgggtcca aatgtgatct caacgggaag ccccgagaag ctgaagttcg gttcctgtgt 120 gacgagggtg cvggcatatc tggggactac attgaccgag tagatgaacc cgtctcctgc 180 cctacgtact gaccattcsc acgtcaagvc tctgccgcat cctctcct 228 261 429 DNA Murine 261 ggaattcggc gcacaccttt aatcttagca cttggtaggc agaggcaggt agatttctaa 60 gtttgatgcc agcctgatct acagagtgag ttccaggaca gccagggcta cacagagaaa 120 ccctgtctca aaaaaacaaa acaaaaaaca aaacaaaaaa aagtatgggc aaaagagaag 180 aaaaatatcc cggaaagaac aatataaaga atgatgttcc ctttgactga ggggctttgc 240 atattacagg gataccggcc tgagacagct gcctcaagac agggacagcg agcctcctca 300 gagtccactt gttccaagtc ccagagtcac cccctatvyc tcgatattgt acctttaaca 360 cmkgttgtta aatggccagg catwtgacaa accagggaaa taagtctata atgaggaaga 420 aattgttcc 429 262 493 DNA Murine 262 ggaattcctt ataattaatt agaggtaaaa ttacacatgc aaacctccat agaccggtgt 60 aaaatccctt aaacatttac ttaaaattta aggagagggt atcaagcaca ttaaaatagc 120 ttaagacacc ttgcctagcc acacccccac gggactcagc agtgataaat attaagcaat 180 aaacgaaagt ttgactaagt tatacctctt agggttggta aatttcgtgc cagccaccgc 240 ggtcatacga ttaacccaaa ctaattatct tcggcgtaaa acgtgtcaac tataaataaa 300 taaatagaat taaaatccaa cttatatgtg aaaattcatt gttaggacct aaacbcaata 360 acgaaagtaa ttctagtcat ttataatacc cgacagctaa gacccaaact gggattagat 420 accccactat gcttagccat aaacctaaat aattaaattt agcraaaact atttgccmga 480 gaactactag cca 493 263 370 DNA Murine 263 ggaattcgga ccaacacgca ggattacatc ttcttcagtt cctagagtcc tctagaagcc 60 tatagaacaa gacggcaagc tctctgggct tgctctgggg ttttgttctg tgttttatgt 120 tgttcttgtt gttttattaa catcagtgtc tcttaagatc caggacccag ggaggtcttt 180 tcacatacat ataccaggac tcttgggtac tactgtcagt cttggggaag caggctcctc 240 catcggcaac caaatccatg tagcactccg aahccttggt tagtcctatg acctaaatag 300 ttaaacttca gaaaatggtt tcaacagatt tcystccgag tggttttgaa attgcatttg 360 tatttgctgt 370 264 338 DNA Murine 264 ggaattcgtt tttggttttg ttttgttttg ttttgttttg ttgttttgtt tgagaaaggg 60 tttctctggc tgtcctggaa ctcactctgt agaccaggct ggccttgaac tcagaaatcc 120 gcctgcctyt cctcccaagt gctggaatta agcaccacca ctgcctggcc tccttttttc 180 ttctgaaggg ttttcccctc ccctttccct ccatcaccga ctgatctcta gcagcaattc 240 ttcttcccgt ttcttctgtt cctcttygga gaggatctca cccttctgaa gaaaggaggc 300 ctgcctctgc ctcccaagtg ctggaagaat tccaccac 338 265 394 DNA Murine 265 ggaattcgaa gtctgaaggc attttagaca ggagactgag aagtactgaa gaatggccta 60 tacagagttt agagcactag csgtagcgta caagactgcg ttcrgttctc agcaccaaga 120 aataaaggtg tcagtsagag taggattatc aagctcttgc tcctgaccga gcacttgtcc 180 cgaccaacac cagtgcacaa cacgtagctg ctgagccttg tggctgarcc cttcckckcc 240 cccatcctct ccatcrctgg acttggtctg cttcttgaaa gcctggactt aagtcctaca 300 gatccttctc tgtgtcagct tctcttttgt cagagtgtcc tctgtgcttc tggctgcctc 360 cgttcccctc tcaatctcct ttctttcatg tttc 394 266 442 DNA Murine 266 ggaattccta tagacacatc atgacaagca tgcccacagg gtactaagct tttcggctta 60 taaaaactag tgcctataac tgtgttgcct ggtccttagc agtcttctac atttgttaat 120 taagttaatg gaagggattt gcacccagct caacctccaa atgaaataat tttgttcaca 180 tatcttagca gcttctagca atcgagtcat aggagttgat tacagagcaa gcgctgtgtc 240 ttcatctctg tgcttctgcc cttaggtcca aaagaagagg atgagcggcc ttwggcttct 300 gcgcctgadc agccagccct tcwtmcagag gtggtaacca ggatgcagtt yccacaggtg 360 ggccatccct cttccagcct gcgagtcaca gccaggkgca gatgggawac aagaagtcac 420 agactgtgag gtcaacaata tg 442 267 341 DNA Murine 267 ggaattccaa tgattttgca attacaacaa tcagtcttcc aattttrrcc gatgaaggga 60 ggaaactttg gaggcaggar ctctggacct tatggtggtg gaggccagta ctttctaaac 120 cacggaacca aggtggctat arcrgttcca gcagcagcag tagctatggc agtgcaggag 180 ttctaattac atacagccag gtaagtcctc ctttgtgtgt gtttdctaaa tgttataatt 240 gaacccagta acccaaatgt agctgagcag tacaacatag ttaacattat aatttcagta 300 aaatggtgga tgttaagtta atatgcagtt ccgccaaatt t 341 268 376 DNA Murine 268 ggaattcctg agccagagcc agaagacctc aacactgtct cagaagatgg agacgccagc 60 ttagaagatc tggaccctga agcagacgaa gctccacgat ccatcttggg gaagccagac 120 ttggattccc aagatctgga tcccatgtct tcgagtttcg acctcgatcc tgatcctgac 180 gtgattggcc cggtgccact agttctcgac ccaagcaatg acacccccag ccctgctgct 240 ccagatagtg gattcccttc cttctgggcc tcactgccac ccccgaaaat cttgggccac 300 cagtccagcg gtgcttcctg cccctgccag tccacctcgt ccgttctctt gtgctgattg 360 tgggcgagcc ttccgt 376 269 322 DNA Murine 269 ggaattcccg gtcataggct gggaggaagc aacagcgaag gtcaggaaca gaggcaaaac 60 actttccacg aattcccctt tcatctgcac agcaacagtc tactagcatg gaagtcgagg 120 ctaggtgcat tctggtccat ctacagtccg gttacctagt tactccctct ccccgccaca 180 cacacacaca cagctgagat gccggcaggt aactgtttcc taagacatat gggtgtcatt 240 tgtgcacctc aggcttgtcc aggaacaccc tatgtvgggc tagacacatg gggcactcac 300 actagcaaag ggcctgtgat tt 322 270 387 DNA Murine 270 ggaattcgaa ggacttgcca cattcttcac acttgtaggg cttccctcct aaatgaatta 60 tctgatgatt ttgaaatact tctttcccca caaagatgtt gccacattct ttgcacgtat 120 agcattttcc ccctggtgag taagagttga gaaatgatga aaacactgcc aaaatctgta 180 tatctatact gatagttttt taaaaacaac atttactcct atttgcattg gtctgtatta 240 atgagatgct atattcaatt ttctgtacct gtattcagtg aactacaatt taaaacacag 300 gataagtgaa agtcacgtag actcccttga acaaagaaga caatggcmac atagaacaag 360 ggagggrata gaatattaaa taaaatc 387 271 103 DNA Murine 271 ggaattcccg gcacaatgga aaaggagata gaaagcrcrc acctctgggg aagaagcata 60 acctcttaaa acagactaaa tvgcagggcc achctgtgaa gat 103 272 527 DNA Murine 272 ggaattccaa cttgtattta aaattcagtg agcattgact gtgtgccttc tgtatacagt 60 taagaccagt tttggtgtgg ctgccatgac accagagggg gttggtggca ttggtggggt 120 gggtgcttag taatgaggtc agagcgactg ataaggcaaa agtaaaagaa gcaaaactaa 180 gtatagagaa ggggtaggca ttcaaacccc agaggacctt gatttaagtc cccatttata 240 gagagtacca tcttgagaga ccttgcaaag ggctttgtgc tgcgttcaaa tgttattgtt 300 tctcttgtac actggatgcc ctcagcatcc cgttaacttg ccaatcatgt ctctcagcta 360 tgctcatctc agcccgtgga tagatagcct accagctttc ttctgtctgg aacttgccta 420 ctgagstgga ccagtcatac catcccagtt cccactgact actacttgcc tctgcagtca 480 cccatggtag tacttagcac agatctatct ttgtaatgtg tttttaa 527 273 325 DNA Murine 273 ggcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt 60 gtcaagcatg acaaagttgc agccgaatac agtgatccgt gcgccctgga cctgttgaac 120 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg gttcagcagc 180 cggcctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg ccgaagccat 240 gctggcggag aatcatacgc attcgtbccg agagccgacg acgactgggc tcatttctga 300 tcgggaatcc cgcagcttca ggcag 325 274 431 DNA Murine 274 gaattccccg gctcgagcgg ccgctttttt tttttttttt tttttcaaat taatatacat 60 tattttatta caaatttaaa aaaaaacaaa aaaatgcaac atcctaaaaa aaatttttac 120 tggtaataca aattcctatg aagttttttt ttttgctagc ataagaaatt aaagaaacca 180 ttaaatattt agaaacattc aacatcaaaa gctttaaatc taactgtagt tgtagcccct 240 gaaaaagcta caaactcttc ttaaaaagta ttttctctac aaagaatctc atcagctata 300 caaaaatctg tacagttttt atactgavgc taatgttgag ctgcacttga atttcacatt 360 cttagcaaaa taattgcctg agcaaatata ctccacactt taggacagcc acttattctt 420 catcctcctc t 431 275 419 DNA Murine 275 ggaattcccg gctcgagcgc cgcttttttt tttkgggggg cttactccag cgatgtctat 60 tagcagagac atgggccagg gaagggtgat ggatacagcc aggggtggga tatcagcctc 120 aaagtgcaga gctttgctct gaatctcagc aggcagccaa agggactgag acaaagctct 180 tcctttcaag ttggcatggc aatcaacttg gaaatcaggt tccccgggcc ttccttccta 240 acaaaggatc cagcctcctc caactgggtc tccactcagc ccctgtagaa aagtbctgac 300 agtattaagt tctactcttc cctaagaccc caggaggtcc tcaccgtgca tagatgtgcc 360 atctgttctt gagaaaccaa agcactttgt agtcttacaa cccataatac ttacagtat 419 276 360 DNA Murine 276 ggaattcgct tgacaacctg caggcaggct ctgggaggcc gagacatcgg cgaagagaac 60 agagagtcgg cggggacaga tctcaagacc agagaatggc aggtgaacag aaaccctcaa 120 gtaacctctt ggagcagttc attttattag ccaaaggtac cagtggctca gccctcacca 180 ctctcataag ccaggtgcta gaggctcctg gagtttatgt ttttggagaa ctgctggagt 240 tggccaatgt tcaggagctt gcagaaggag ctaatgcgcg tatttgcagt hctgaacctg 300 tttgcctatg gtacatrccc ggattacata gccaacragg agagcctgcc agaactgagt 360 277 337 DNA Murine 277 gcgktaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca 60 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttyggccag 120 tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcsccggct gctgaacccc 180 caaccgttcg ccagtttgcg tgthgtcaga ccgtctaccc gacctcgttc aacaggtcca 240 gggcgyacgg atcactgtat tggctgcaac tttgtcatgc ttgacacttt atcactgata 300 aacataatat gtccaccaac ttatcagtga taaagaa 337 278 334 DNA Murine 278 gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca 60 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag 120 tgcgtcgagc wgcgcccgct tgttcctgaa gtgccagtaa agckccggct gctgaacccc 180 caaccgttck ccagtttgct gtygtcagac cgtctccgac ctcgttcaac aggtccaggk 240 cgcacvgatc actgtattcg gchgcaactt tgtcatgctt gacwchttat cactgataaa 300 cataatatgt ccaccaactt atcagtgata aaga 334 279 419 DNA Murine 279 ggaattcccc ggctcgagcg gccgcttttt tttttttttt tttaatttaa ataaataccc 60 cgctcctccc tccacccgct tacgttctcc ctcttccccg aacatcccac ccatccctgg 120 ctagaccctt accccagaac taaataaaat gcctgtttta cagcagacca cactcactac 180 caaattctgg gaaaactata aatactgtca ctgtctgggc ctctctgcct tctgactctg 240 ctccggaggc agccacattc cctccctccc gttgactggg caaggatggc agaggcctgt 300 aggcactggc cttbgagagt gcaaatttag ccttgggttc tccacctcct gctcaggagt 360 aggtcagaag ggccccagaa attccctcag actaaaataa atagcaaaat aaataccct 419 280 141 DNA Murine 280 ggaattcgca ggtcgccggc gagccgcgtc cggagcccgg cgccgagcvg gcctggggag 60 gcggaggcca caccccgcbc vcgcccaggc bcttcccgcc ggtgaatcat ccccgcagca 120 gcsgctcccg cagtccgctg c 141 281 150 DNA Murine 281 ggcggattct ttatcactga taagttggtg gacatattat gtttatcagt gataaagtgt 60 caagcatgac aaagttgcvg ccgaatacvg tgatccsmsc gccctggacc tgttgaacga 120 ggtcggcgta gacggtctga cgacacgcaa 150 282 265 DNA Murine 282 gaatactttt atttagattt tattcataaa ttaagttgag agcvmttatt tgtaasghvg 60 ctctatttcc cttgtccttt cgtactggga gaaatcgtaa atagatagaa accgacctgg 120 attvmmmcgg tctgaactca gatcacgtag gactttaaam cgttgaacaa acgaaccatt 180 aatagcttct mcaccattgg grtgtcctga tccmacatcg aggtcgtaam mcctaattgt 240 cgatatgamc tcttaaatag gatta 265 283 362 DNA Murine 283 ggaattccgg agtctccatg ctatgtccca ggtgattcct ccacagtaaa acggggagac 60 ctctgggttg gagagtcagc gctggtcact cttcattcac ttgcagggag cctcaaggtt 120 aacagagctg ggcttctgtg agcagcatgg cctggaatgg ggtttggcat ggtcagcgta 180 agatggtcga gaaggtggat ctaaggaccc ttcctagcat ggggcaggaa aatagaggtg 240 gctccaactg ggccttgagg ccctagaggg ttaagtgcgb tctcacagga accaaggcca 300 agyctgggcc acagttdaga gacattccac aaaccctgat ccaatgawtc aagctataag 360 cc 362 284 392 DNA Murine 284 ggaattccac kachagggga cttgttggtg gtccccttct atctgaatct catactcaga 60 cacgctccca ctgctccccc gatctgagtg cccctcttcc tgcaagcggc tccgaagggc 120 tttgttgggg gttgtctcca tccgaagatc actgctgact ggaggctgcc gtacctgagg 180 gcagtacgga ggggagattt caacaggatt ggtgaagaag ctgccatctt tcacccathc 240 tgttgaaatc tccccttcta tctgaatctc atactcagac acgctcccac tgctcccccg 300 atctgagtgc ccctcttcct gcaagcggct ccgaagggct ttgttggggg ttgtctccat 360 ccgaggatca ctgctgtccg aacctccccc gt 392 285 382 DNA Murine 285 ggaattcgtg tgctttgagc tttactaaag tttctttagt gaatgtggct gctcttgtat 60 ttggagcata gatattcaga attgagagtt cctcttggag gattttacct ttgatgagaa 120 tgaagtgtcc ctccttgtct tttttgatga ctttgggttg gaagtcaatc ttatcagata 180 ttaggatggc tactcctgct tgtttcttca taccatttkc ttggaaaatt gttttccagc 240 ctttcattct gaggtagtgt ctatcttttt cactgagatg agttyctgta agcagcaaaa 300 tgttgggtct tgtttgtgta gccagtttgt tagtctatgt ctttttattg gcgagttgag 360 accattgatg ttaagagata ta 382 286 258 DNA Murine 286 ggaattcccc tccttgactt cttctttccc agctggtttc gaggtctcag cagacttggc 60 attgcccaca ggcttctggg gctcagcagg ttgtkcgttg gttacaggtt tcagggaccc 120 tgaaggctgt kcgttggcta tgggtttcag ggtctckgca ggcttggtgt tgcccacagg 180 cttcagggtc tcggtaggct tbgcattacc tataggtttc bgggtctcag caggcttkgc 240 attgcctacg gtttcagg 258 287 643 DNA Murine 287 ggaattcatt gagatcgttc aggaaactat gcatttccaa gccattatat agtctgggca 60 agataagttc ttatttcatt tgtctaatac tcatgttcaa gggaggccct ggttcagtct 120 gggcgcaggg ctcgcagatt acaccttaca gcctctcatg ttcagataac tggcaacaaa 180 gcaataaaaa gccgtccaac ttgtcagtgc gtagcagcaa agcccttcat gtgggcagga 240 caaagggctg gctctcatta gatgattagc tcattcaggt cacatctagg tcacttccac 300 ctttgtctgg attccaaggt tagccctcat ctaggtgagg ggatggggcc cctgtgaagt 360 cctcagagct caccctggag agttaagatg ggcacaatga gaaacaggag agcagggtat 420 gttcctcacc agagccagtg ttggcacact ggctcaatct caagaggttc cccaaatgag 480 tcagatttat agctgacatc aaggacagcg tcagagactc tagtctgtga aatcatcact 540 ctcaattgag ggagaccaga acctagggta ccacccaggg aatgtcaatt ccgatagaca 600 caggrtcggt agccagtgtg tgtagttagg cttcggactg ttg 643 288 424 DNA Murine 288 ggaattctcg agcggccgct ttgtttgttt ttccttgata ttaagtagtg acagttttct 60 ggatgcaaaa ccacagacgc atcgccttca gtgcaacagt cctgcgggat gatcggcctt 120 ctccaggggg atgttggctt ccaggcacat tttcacaaag tcctggataa cactggcttt 180 ctctgtttgc gcaggactgt tgcactgaag cgatgcgtct gtggttttgc atccagaaaa 240 ctgtcactac ttaatatcaa gggaaaacca accaaccaac caaaaacccg actggaaatt 300 aagctgaaga accttattca gagacaaaat ggaacgattt gttgtaacag caccacctgc 360 tcgaaatcgt tctaagactg ctttgtacgt aamccctctg gatcgagtca ctgaatttgg 420 aggt 424 289 309 DNA Murine 289 ggaattccag tgggattcct cagctccatg atgcaatggt tatctttttg gtaaagaata 60 ttcaagtcct gacatcatag tagtaatgga tattactcat ggtatgctct caagcccagc 120 atggcacatt ctgtaccctc tttatcactg aagtaagcaa tgggtttaaa aataacgttg 180 cttacacacc cagagtacca atgattcatt aacaactgaa caaatactgc tctggactcc 240 aaaattatta cagaatttta tatacaggat tttgaggcat agggtatttt ccacccctag 300 tagaagtat 309 290 325 DNA Murine 290 ggaattcggt ttttaaggga attaagtcta tgttgatagt acagggggaa gaggatataa 60 aagtgaattt atagttttcc cagaccacaa ggcattgttg tgccttggtg gccacctagg 120 tcaagaccag gatctctctc ctggggagcc aacaggagcc ttccaaaatt atcagggaaa 180 gaggttttct gtcctcaatc cagcttggga gagattttgt tactgacaca tgatccttcc 240 cccacccagt aatgaagtgt tctgtgtgct aacaatatag gcttaaaaaa aaaaaaaatc 300 bsgccgcbaa tttccaccac actgg 325 291 390 DNA Murine 291 ggaattcatt gaaccccatg caattatagt gggtacttca atacccctct ctcaccaatg 60 gataggtcat tataacagaa actaaagaga aaagcagtga aactaataga tgttataaac 120 cgaacaaatc tgatatcaat ggaatttttc atcgcaaaac aaaagaatat gccttcttct 180 cggcacctct cagaaccttc tccaaaactg atcatataag tcagcaggaa gtaccaacag 240 gaacaccagg agttctcagc tgtgcatatc tcagggaagt aaagatcagt gaagattcga 300 aaccattgca cagctagctg taccagcaag actgcacagc tagctatacc agcmagacta 360 gctctgtccc caccactcca tggaatctta 390 292 335 DNA Murine 292 ggaattcaaa gaggcaaaca tagaatcaaa ctaagcagtg ggttctttgc aaacagttgc 60 cttcatatta cctcagcagt taaacgtttg tgtggagtac taaggtggtg gtggagtgtg 120 ctttgtttag ttcttttact ggagtgggca ccccactttg tctctctcct aaagccctac 180 tcactttgta tcactgtagc cagaccacaa aggctgtatg ttgcaatgta tcaagtgaca 240 gttttagtta aacataaata ggcccattga accctgccaa acctggtcat atagatcaag 300 gtcaaggtaa aataccaggt ttctgtagta ggggg 335 293 369 DNA Murine 293 ggaattcccc ggctagagcg gccgctcgag ccgggtcgag cggccgcttt tttttttttt 60 tttttcacgg gaacagactt tattagttca cttgggtctt ctctggtacg gcatttgaag 120 ggttctctgg caccccctca tttttttctt ttttggcagc agctgcagca gcttttaagg 180 cccttttttg cttcttcagc ttttgcacct cctggtaaac ccgaatgcac agagccttct 240 tggccaggaa gcvgcggtga accttttggt aaatgtcaga ggggggtaag gtatattcca 300 cccctagctc cttgcatgtc ttttcgaaga catcatagtt ggtctgacgg aggattttga 360 gcaactttt 369 294 394 DNA Murine 294 ggaattcatt ttataattat gaatcatgaa tatctgtatt tgccgatggt ctcaggtgac 60 ccttgtgaaa gggtcgtctc acccccaaag ttctgtccac aggttgaaaa ccactgtgtt 120 ggagggtgct gactgtaggg caacaacctg aggacaaaaa aaagccttga acatgtgttg 180 ttgctctggg agctgtgtgc tagctcatat cttcgccagt cctcccacta agcttggctg 240 gttcggggta ccccctattt atgggacyca gggtaggggt gaggcagtga tggkgccagt 300 ctgctgcact gcccaagcag tgaccgctcc cttgatctgt gctgactgtt aagagtgaak 360 kkcttcagaa agtagtactg ccacagccac caga 394 295 536 DNA Murine 295 ggaattccgg ctcgagcggc cgcttttttt tttttttttt ttagttgcaa gcagatcaca 60 aatcctctta gatgtaagga aagtgggtgt tctggagagg actcagatcc tgaaaatgag 120 gaagtgagaa tggcttttag ccatttttgg aaagtacagt ctgtaatagt ttaccttctg 180 gcccagagaa ttcacattct tctgcctgaa caatgcagtt aatttttttc ttctacaaac 240 ccctatggta tcagctggat gtcagggttt taccatttaa acctgatcca gtcacagaaa 300 tggttgttta ttgcagatga tactcctcat atgaaagaaa acctatgaaa caaaacaagt 360 tagcagctgc ccatatattc tacatatatt gagagaagta taagacagtg tattaaacat 420 gagaaaaatg gaaggcacac agcagacact gttctataca gtttcaattg aagtccaggg 480 tatatgttga cagctgggtc aactcctact ctctgcagta tyctccaaca awcccc 536 296 244 DNA Murine 296 ggaattccaa gaatgtacgc cagaggaacg ccacctgagt ggtggggcag gcgggggagg 60 ggaggtgccc agggtgcctg accccaggcc agctctacct ccactccagt atcccatcct 120 gtcccgattt gaacctaccc aacccaacct atcccaaccc aagtgaagac agagccttac 180 cttacagaaa acccacctgg aagaagcaar ccacttcagc ccctgtttct aatttaaact 240 aaat 244 297 331 DNA Murine 297 ggaattcgtg aaggtatgtg acaacgttta cctgactaaa gcagctatca gcttacaagt 60 tccctgcttc cccagtcaat ttggtgactt tcattcttag tgcttcgacc cttttcctac 120 agcaagcaca caacactgca gttctttacc ctgcaatcct atgtatttgc ttcaattttt 180 gttctccaca tcctcaacta tgcattattg ggacagcaaa aaaaaaaaag aaaaagattc 240 tttcttctaa gggagaagta agtcacttag ccttcactat agaccacctg ggcacagtgc 300 acaagaaacg ccgagctcat cctttttctg t 331 298 308 DNA Murine 298 ggaattcgtg aagagtactg ccttgtcctt tggcgtgtgc atcggtcctg ctctcacccg 60 cagcctgcgc tctactgcct gctccagtcc actcctgacc gacagcatca tggctacgag 120 aggcactgtg actgacttcc ctggatttga tggcagggct gatgcagaag tccttcggaa 180 ggccatgaaa ggcttgggta ccgatgagga cagcatcctg aacctgttga catcccgaag 240 caatgctcag cbccaggaac ttgctcagga gtttaagaac tctgttttgg cagggacctt 300 gtggatga 308 299 491 DNA Murine 299 gaattcccgg ctcgagcggc cgcttttttt ttttttttaa caaacccttg tgtcgagggc 60 tgactttcag tagatcgcag cgagggagct gctctgctac gtacgaaacc ccgacccaga 120 agcaggtcgt ctacgaatgg gttagcgcca ggttccacac gaacgtgcgt tcaacgtgac 180 aggcgagagg gcbgcctctt cataattttc aatctgttcc acttgtcttt cccatctgtc 240 taccatgtac ttgtacatgt agtcatggct taggtgtggc ttgtgacagg tgggcctctg 300 ggtttcccat gctcaaggca agggaaactg tcttacttaa cagtgtgtgt ctaaaaaaat 360 ctggcttttt tgagagtgca gtatttaaaa aacaaaactg tactatcaat ttctataaag 420 ttgttcgaga atttatatgg gtcccaaatg tcctttctga ctgaagtctg cagtaaadcg 480 aattccacca c 491 300 465 DNA Murine 300 gaattccggc tcgagcggcc gctttttttt tttttttttt gattagctct ggataatttt 60 ttatggggag gggaaaaagg catttgatat cctgcctttc ctacagcact cagattaaaa 120 cacaggctta aattaattct gattgcttcc ttttccttgt tccttcctgc agaggctgat 180 gggacagtgt ccagggctgg agagccacgt gttctgtaga tgataaataa ctatgaacat 240 ttggtgctga attttttaca cttgtctctt gtggtgctat tgtccggaga cccttaggtg 300 gscctagggt gcctgccatg cctcattccc tcgaattcca ccacactggc ggccgctcga 360 gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt 420 cgttttacaa cgtcgtgact gggaacaccc tggcgttacc caact 465 301 413 DNA Murine 301 gaattcccgg ctcgagcgac cgcttttttt tttttttttt ttttttatga aatgagttca 60 tattcaagtg tgactatgta gtcaagtaca tagttgaaca tgagtagcct catatcataa 120 aagtagtctt ctatcattca tatacagtat atatcatttc tatacactcc tttgctctat 180 actgtgcctt ggagatctta agtcatgtta tcatcttaaa gtgtgtcagg gtagttacct 240 acctcaggca ttcaggttat ttctagtttt cagcacttwc aaataccttt agtkagtatc 300 tttgtgtgta ctttttcata tgctgtgtaa cagtttctta agcaggactg caaaaatgta 360 aattkctgct tttcagctta ggkcatctaa cagatacact ttccttcaaa agc 413 302 436 DNA Murine 302 gaattcctca gacctggagc aggcgcggcc tcagacttct ggagaagaag agctgcagct 60 gcagctggcc ttggccatga gtcgcgaaga ggctgaaagg ccagtccccc cagcctccca 120 cagggatgag gacctgcagc tgcagctggc tctgagcctg agccggcaag agcatgagaa 180 gggggtgaga tcctggaagg gagatgactc tccagtggcc aacggcgcag aacctgctgg 240 ccaacgtcgt caacgggaca gggagcctga gagagaagag agaaaggagg aggagaagct 300 gaaaactagt cagtcctcca tcctggactt gctgacatct tcgcacctdc cccggccctg 360 ccttccacca ctgctctgct gacccatggg acatcccagg tctcaggccg aacacagagc 420 caagttvgct cctctt 436 303 484 DNA Murine 303 gaattctttt tttttttttt tttttttttg aggtgctgag tcacactgtt aactgcttta 60 ttgagattca gggagatcct tcccccaaga gacaccacag tgtgaaaggg acgctgcctc 120 ccgcccggtc agtccatctg tccatgcctt catttgatca aatgtgcacc cactatccac 180 tggaaacagc ctccaacctg tccccatttc ttttcccctt agttctgaaa aataataata 240 ataatgacaa caaagaaaag aaaaccaaga tgcagtagtt ctgagagatg attgtacaga 300 cccaaagtgg gacgcatgag aatagaggga acacttgaga gtaaacctaa ggccaaggag 360 agggtatgca tggctcagaa aacacgtact ggggaagagc ctgcttaatc atgtgcatgt 420 tgggtgcaca tgcctctgct gaaagaagac aggacatcag ctaggcagac aactgtatcc 480 cata 484 304 577 DNA Murine 304 gaattccaca ccttgtaagg atggtataac ctctgcctta aacaagttca agaaaaggag 60 gggcaaaaag agcgcttgta tgcagcttta attatctggt ccccctcacc ccctgccttt 120 tgctgtgctc ttagccccag gccaaaggct aagactggaa ctaaatttgc ataactcacc 180 tcccacatag gtgtccttgt ccactcctct tagccttcgt gtatccggag cagattttat 240 agctgtgcag tcttactcca ttgctaccta agggaaaatc tgttaggtta aaaaattatt 300 tctgtcccat ggctggattt tcaaaaccaa ctgtggaaat aggctaatga gactggtaaa 360 gccaaccaga acacccacac gctattccca aatcaaatgc gttgtaaatt gggcgaatct 420 tgtatttgta gctgtctggt aatgtgaggt cagattttwa gcattctatc atcatgaaat 480 tgcactgtca ctttccatag cagccgagag aatgatagtg aggttaagga gccataaccg 540 tagaaaatga aggtgctcma gggcatgaat gttctga 577 305 492 DNA Murine 305 gaattcgcag atgggccaag agcttcaagg agaaatagtt gtaataattg cagatcagta 60 tggaaatcag atttcatcat tttcacctga ttccttatct actttgtcga ttactggaga 120 tggccttgac agctcaaact tgaaaatcac cttggaggcc aactcacaga gcgtaagtgt 180 gcaaggcatc aggtttactc cagggcctcc tggacccaag gatctgtgtt ttacttggcg 240 agagttttct gactttctgc gcgtgcaact ggtttctgga cctccaacca agctgctgct 300 tatggactgg ccagagctga aagagtccat tcctgtgatt aatggaagac aattagagaa 360 ccctctcatt gttcaacttt gtgatcagtg ggataatcct gctttagtcc caaacgttaa 420 aatatgtctc ataaaagcaa gcagcttaag gctactacht tcaaaccagc agcataaaac 480 sgattccacc ac 492 306 611 DNA Murine 306 gaattcgaac tctacaggac aacccatttc ctgagagggt aggccagatg gctctgggtg 60 actgagaatg tcattccttg aatgggggac agaacggaga gggggtggga tttgtggaca 120 cattcacata taagcatatg caccccagca acaaggctcc taatagcctc tccaggaagg 180 agacaccgac ccctagattc ctggagtgtg taaacagccc acccctagag ccctcatcca 240 gtccatttct ccagctcgca agacccggct tccaacgtga agtcaccagg gcgtagaaag 300 tccctcctga tattcacatg acagattcct tttcgaacgt ggcactggag tccccggtgg 360 gtccctggta ctgtttcagg aggggattcc cctcctctgt ggcgaggggc agtggattca 420 gagacacctc gttcttcacc tggatcaatt cgggctctga gctcggcatc ttggttcgat 480 ccacgtaact ctgaagcagt ccagccccaa aagcatcacc ttccacgttg aggacagtac 540 aggacctgtc cactagccag tccacgccaa gatcaaggag atgtccttca cagggcaggc 600 tgacttsttt c 611 307 484 DNA Murine 307 gaattcctcc agtcggttag ccggaaaaac gggtgcttct tgacatcctc tgcatccttc 60 tcaccagctc ccaggcgccg ctcaggattt ctccttagca gccttctcat tatggaaatg 120 gcttctgtag ataagaacct tggatacctt acttcgtcat ttacaatact gtcaaaaacc 180 tcttcttcat catcaccagg aaagggagac tcgccgacga gcatctcata tatgagtaca 240 ccaaggcccc accaatctac agcccttgtg tacgatgttt ctgttaggac ttctgggggc 300 aagaaactca gggagtacca caaaatgtgc ttgtcctatc tccatacccc attccttctt 360 tgcaaagacc aaaggtcagg caattttcac aaagccttct gtatctagca acaagtttat 420 ccaacttcaa atctctataa acaattttgt gttcatgtaa gtattgcaac ccaagaacta 480 caca 484 308 460 DNA Murine 308 gaattcaacc cggctcgagc gccgcttttt tttttttttt ctaaggacct tagaaaaata 60 aaaaaaaaat tctgagtgcc atctttatca tctcttcatg tgtgtgtatg agtgtgtgtg 120 agtgtgtgta tgtgtgtgta gtgtgtgtat tgcatgtgtg tgtgtgtgtg gtatgtgtgt 180 tgtattgtat atataccaga ccatgaggta ataggagaat acactattct cgccaagatt 240 tttatcttgt ctaatcaagt catgtttctg gctagaacac ctttcttgta atcattttaa 300 atgtagtcat ttaaatgaat aatccaaaca gaagtcctat tagatccatg tttctgttaa 360 atgattgcta agccctaacc tttcatttcc cttcaggaaa scatcaaaag catggttatc 420 attcactcta gaagcccgga ttatcgtttt aaagtcatca 460 309 213 DNA Murine 309 gaattcctgg taagggcaag tcatacatgg aactcggttc ttcacggcat gcttagaaac 60 actgcgttgt ggagcttgtt tcgtgtttka aggaattcta acgcactaac acataatgac 120 tctagccyta kgatgcacag gcaaaaagga ggcctaagga ctcacttaca cactgcaata 180 aaagcttkct ccacttgttc tccaggaatc gcc 213 310 207 DNA Murine 310 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg 60 tcaagcatga caaagttgca gccgaataca gtgatccgtg cygccctgga cctgttgaac 120 gaggtcggcg tagacggtct gacgachcgc aaactggcgg aacggttggg ggttcagchg 180 ccggcgcttt actggcactt cwggtac 207 311 285 DNA Murine 311 gaattcgtca agttggtctt gaactcctga gttcaaacaa ccctgctgtg gaatccacgg 60 tagctagacc tacagatggc atcaccaagg tcagcttgaa cacacagtta aaaatcatta 120 accccaaact gaccataatg tatcaaagat gggtaggaat ttaatagcct gtctttatgt 180 ttaaaaggtc aaccaagtaa caataatcaa gatatctgaa gaagtctgcc aagagagctg 240 gtgcttcctg taagctcaca ggaagacgag gagcttcaac ccaaa 285 312 457 DNA Murine 312 gaattcgtta tttcttaaaa taaaaagaac atctaaggac tgagtcctat atgcacttta 60 gagcatttct acagcatgcg attctaagag taaccccacc caatatggca aacaatcaaa 120 ttgtttaaaa tttaacttag aaagtctgag atcattattt tcaaaacatt gatttgtaca 180 ttgtttcata cacaaataac caactgacta tccaagcaca ggacagggca cctctctgga 240 gaaaaaaaat ctctgacagc aggggcagga cggctagtgt cacatgacta caaacgtccc 300 tccaacttca caggaaaccc aaggaaagaa cagaaagtgg acagtgaggg gacaggaggg 360 acaggaggga gggaaavcag ctygggagta agtcmsctgc ctgagcaagg gaaggaagga 420 ctctgaccaa gcattcgtgg scmatcctaa catgtgc 457 313 418 DNA Murine 313 gaattcgtcc tctcttggag gtctgctcct ttttgaagag gaaacgggtg agagggtgtt 60 caataatgga gaaaagagga taggtgaagt ggggggcatg gggcatagct aggaagactg 120 tagggaggaa aaacaatgct caggatatat tgtatgagag agaaccgagg cagtggtgga 180 ggtcagggta gtacaaatta cggaaagagc cagcgacgtg gtggtcatca gaataactac 240 aagccatact gagaggcagc aggagcgccc gagtgacgac cgcacacgct ttgtttggac 300 gcgggaattc caccacactg gcggccgctc gagcatgcat ctagagggcc caattcgccc 360 tatagtgagt cgtattacaa ttcactgvcc gwcgttttac aacgtcgtga ctgggaaa 418 314 450 DNA Murine 314 gaattcctta ttttcagatg acagttttcc tccttttgga tcactgctac tgcggtgttt 60 tttagtaggc aaagtaagtg aatttaagat acgattcttt acaagtttgc tggagccaaa 120 aaagggaaat gaatttttat cttttatggg tccaggtcgg tcataaaatg ctggctcagc 180 atcttcattg atgtcaagga aaaatgtgct ggtggaggtg ctgccgaagc ggtcgtcctc 240 cagcatgaac atgcttgatg gtgcagactc actctcactg ttatgtctag agctggtcga 300 ctcagagttc aagctgaggg tgcttgggac agatgagagc tcattgcaga gctgctccac 360 atcatctgga accactggcc atagahchth cactgtsctt acagaatccc agctgtgaca 420 tttcaaaata tcacagcctt accttggttt 450 315 555 DNA Murine 315 gaattccact actctgccaa ttaaaaaaga tttgtttttg caaaagttat gtttggagaa 60 aaataaaaaa gcttatggtc cttgtattaa gcaaaataag gtaggctcag aaagatgggt 120 gctgttttct cagatatatg aaatccacac ttaatagtat aagattttaa gacgcagaag 180 gtactattca tttagaaaag ggaaagtaac ctgtgggggc cagtacagag gacgaaatga 240 ggatgaacaa gcttgaattc cgaaataaag ctgtgtgtga atgtcacaaa ggttctatca 300 tactgaccaa tgagtgtatg ctaatcaaag taagattcgt taaaatggtt tgagaaatca 360 ttgttgaaat gttaatcaat ctcatctgaa gctccgtcta gatttttatt ttttatagaa 420 cttttataaa ctcttccacc tcaagtycca aattggaaag atttactcct cctttcataa 480 gttycccaag atgagataag agcyatrcaa wggtttgttt gggaaattga ggcatggaca 540 tcactacatg ggctt 555 316 172 DNA Murine 316 gaattcgcgc agaggaactc tggtatcgat ggtacaagaa gagaccccat gatcatcara 60 gacagacara ggccagctgg ttccagactg gcttacaggk aaaatccagc tgctgcttgg 120 gcccctgatg gtcgacccag tagagggatg gattcagggt awcagccttc cc 172 317 355 DNA Murine 317 gaattcttga aatttaaaga aaaaatttat tgaagatctg aaaaacaact cctacaagat 60 tgacttttcc ataaaactgc agctacacga tgcattgcgt ctatcatgtt aaaacgtgca 120 ttagacacaa atacaaaacc catgaaaaca agccaccatt ctttaacagt tgagcaaaga 180 taagatgcct aaggaatgac atggatgact tgcaaaggat gggctcttta agcaccatta 240 waaaaaaaaa waagagcaca gatggatgag tgttcagtta tatacactga agtgaacctt 300 tggcactagg aatcagagca wttgtcataa gaagcattwa acacatatta taaaa 355 318 425 DNA Murine 318 gaattcaaaa acctttaatg agtaaaagac agtgtagggt ttgtgcccat tgtccatgtg 60 ttgctcctat tgtcacccct cctatcagaa ggtatttttg atgcgggcvg ccaccaggac 120 taggatttcc ccaatcttcc tctgccagtt ggtgatatcc ttggacacag cacaccacag 180 ctctccatgt cggggctctg cattctcaca gcgtttcctc acctcctcct gttgctcctc 240 agttccatgc tgcagttcaa atttgtagaa gaaggcccag gcatcccccc agatctgagt 300 caatcttcac agtgcsatgg aaccactccc gavccytggt gatctttctt tcactccaga 360 acaacttagc cacagctaaa agcacatgvg gtcatgttca cacttcttca gggcatccac 420 actct 425 319 251 DNA Murine 319 gaattcatgg cgcatcccgc acccctggcg cccggcgccg cggccgcgta cagcagcgcc 60 ccgggggagg cgcccccgtc cgccgccgcc gccgccgccg ccgccgctgc tgccgccgcc 120 gccgcggctg cccgcgtcgt cgtcgggagg gcccgggccg gcggggcccs cvgktgccga 180 ggccgccaag caagtgcagt ccctgctcgg cggcggcaca gagctcgtcg ggggcccccg 240 gcgctgccct a 251 320 320 DNA Murine 320 gaattcgttt ctgaaaaata gctacagtgt acttacatat aatacataaa tctttaagaa 60 aaaaaaaaaa aaaggggaga tttaaaagta aaggcctgaa tgtctgttca actaactaaa 120 tttatagaaa gcttcacagt acaaagcaag caactgactt aagacttgca cctaaggctg 180 gagagattgc tcagaggtta agaacactga ctgctcttct gcaggtccca agttcaattc 240 ccagacaacc acataggtgg ctcacaacca tctgtaaaca agacctgatg ccctcttctg 300 gtgaactgaa gaaggctaca 320 321 374 DNA Murine 321 gaattccggg gcaccctctg ctgaacagta ggggacgggc caggtggcag agtggccaga 60 ttggggggtg aggccgtgga ggaaggggtc ccagctccag ccccgggccc aggactcacc 120 aggctttacc acactctgac actgctcaca cctgggagtt gcttctgaga agatcttctc 180 tttcatccag cccatcgtgt attcttttct gcaggaggtg ttgacacagt gtgatgtgta 240 gaaggtgccg tgggcctcca ccaggtcctg gggctccagc cccgccactc gttccagcgt 300 gtctatgttc tgcgtgtagc agcgcagcag ctagcccctt htccttcagc aggccggatg 360 aagtaattgg caga 374 322 208 DNA Murine 322 gaattcactt acactgtcta ttccctgaac gaccagccgg ggctccacct gggcttcgag 60 gctgccatta tgcctgccac aagtgacagc cttccctggc tacccaaggg cacccaccga 120 gcaccctcag gttcagctgt gctcacacar gggtgaatga gcaccccagg gsayccactt 180 ttgggttcta ccactbcgat tcccacca 208 323 396 DNA Murine 323 gaattcggca gacaaacagt gaccagaacc agtgccctaa ggaaaacaac ctctacaaac 60 cactgaagcc acttgaaact ctcggacgaa tgtgctgggt ttcccacaac agcgacactt 120 cccagagagc tactgacaag gagccctcag gacactgatg tgcatccttg gacttgctca 180 ctcaggcccc tgagtcagag cctgccataa tatccatccc taggcctgct aacacacttc 240 caggataaca gggaggaaat gacattcaca cgttaccttt tgtgatctgc hgccaccavc 300 tgttggtttg gaggactcta camcahhttt ctttvcccag agattgggga agatcccact 360 aacttctgtg tagcaaagcg ggggctggtc ctggtt 396 324 585 DNA Murine 324 gaattcctga acagaggttc tcagaacata taaaagatga aaagaacacg gaatttcaac 60 agaggttcat tctcaagaga gatgatgcca gtatggaccg agatgataac caggtgaaga 120 atggaagagg gtgggcctat aaagagagaa actgggaagg gagaaggatt tgggggaatg 180 gaaaaaattg aaaatatctt aaaatggaaa actacacagc gctgttctcc tgagttgttg 240 gggcttccca ctgaggactg gctacagttg ccgtgctcaa ggccccagag agacagggtg 300 ctgaggtctc atttggccca cagctcttta ggtttgcctc taacttgtaa ctacgtttca 360 ttttggacaa acaaggtttc tccctgtgtc agccttgatg tagctgactt cagtgtcatc 420 tctttgctca acccctccct gtcttgcaga atttacactg ggagctacca aaataaccaa 480 aagttacttt atcccatttc cactcttcta gccaagggct ggccttaaah gcaaagttat 540 ggtctaattt aaccagttac agaggtgtgt ctttgatccc ctttg 585 325 389 DNA Murine 325 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg 60 tcaagcatga caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac 120 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag 180 ccggcgcttt actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc 240 atgctggcgg agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt 300 ctgatcggga atgcccgcag cttcaggcag sctgctcgcc tacmgccagc acactggcgg 360 hhchcgagca tgcatctaga gggcccaat 389 326 375 DNA Murine 326 gaattccttg cactatgcgg ctgctcgkkk ccacgccaca tggctgaatg aattgctcca 60 gattgccctt tctgaagaag actgctgtct caaagacaac cagggataca cgccactgca 120 ctgggcgtgt tacaatggta atgaaaactg catagaggta cttttggagc aaaaatgttt 180 tcgaaaattt attggtaatc ccttcactcc actgcactgt gcaataataa atggtcacga 240 gagctgtgca tcattgctcc tggkggccat agatcccagc attgtcagct gcagggatga 300 caaaggcagg acaaccctcc actkggcagc ctttggagat catgcggagt gcttgcagct 360 gcttctgaga catga 375 327 532 DNA Murine 327 gaattcggaa aatgaaagag ccttcctgtc ttcaacatat ttttgtttga gcttgatgtc 60 tgccaaccaa gtactcatag tagtatcagt atcactgtta gtatccacat cagtatctta 120 attccatgac ttttcactcc acccaactat ggctcctcga ttttcttgtt taagctttct 180 gaatttcttt ccagtctgaa atgctaatga tgccctcaga ctccttccct cttgccacat 240 ctccctcttt tttgaactcg tctccccctc tgtgttcata cccatcatac tttgctaatt 300 gctacttctg tgtcttaatc ataacattct tcttcagtct ttaaacaaga tctgtcccag 360 agtctaaatt tagccatttt cactctctgt gtgtcccatt tgggctttga attaaagttc 420 tgagttcact ggctttcatg agggggaggg tcacagaata aagtttccag tgtgttgctc 480 ttgaaaggag atctcccata ttcaaatacm cttctcccta aatattctgt ta 532 328 314 DNA Murine 328 gaattcacgg atttaacagg aatagaatgg cacaaggttt aatcaccagg gaaataaagc 60 aatcacaact gcggctcggg cgctgcggcc ctgctcacac cgacagaact gcggctacac 120 agagattgga aaaccgctac acgcgcctgc ccctacctgc gcccacggcc atgcgccccc 180 acctgaacta aggcagaggc aagcatcccg gagacttcac cccacaacct tctgagtctt 240 agtcttcvtt ctgtgtactg tgacaatgta tgaatcaact cttctcaatt cacttgagtc 300 caagtcgtaa ctga 314 329 342 DNA Murine 329 gaattcgcgc actgacaggc cactgtrcac gtgtggaggt cagaggtcaa tgatagaarc 60 cctctccttc accacatagg tcctggaggt taaactcagg ttgttagact tggcaacaag 120 ccctttgtcc tgctgarcca tctcactgcm ccrccaccct ttwctgagag aggctcttca 180 ctatcctaac ctaggttacc ctggaactta tgatgcaccc aggtgctagt gttcacaact 240 gggaggaaaa cctcaaatta gggttatgtg aactgtaaca taaatttgta attttaacta 300 cttdtttttc ttactgggtt ttgatataaa dcctcacttt gt 342 330 412 DNA Murine 330 gaattcgccc cgactagtca ctgtttagaa agaaagaaga aaggaaagac ccagcaaacc 60 taagctagta tgactatcca tctaaaaaag gctagggagt tgtgtggtgt ttgtgtgtat 120 gtttgtgtgt gtgtgtatgt gttttatgta taagtcaagt attcacaaat cttttcacac 180 tagctgccat aaaaagacac agacattaca caaaaccata ttgcttttca tatgcactct 240 ctgcagttcc tagctcaggc tcaaagacag cccacaaaag agtaaaagga acatgttgga 300 aacagaagtt ggggaagtcg gagaacctct gcagactkga ggtcgaacat ggagacacag 360 acctcacaga aacacactgg ccagctcctc artkcacaag tctkcctaag ct 412 331 275 DNA Murine 331 gaattccaag agtattagac attttggaag attattgcat gtggagaaat tatgagtact 60 gcaggttgga tggacagaca ccccatgatg agagacaagt aagtatgaaa gggtgggaag 120 ttaaaaagtg aagtaagaac tttatttttt atattccatt agktgtacca atttaatata 180 atgtttgtat tgtattgcat cagagtattt gatttttttt aaaaatatgt attttctttt 240 aaaatttaat ttggtgtgat agtgttttgc cyaag 275 332 397 DNA Murine 332 gaattccgcc aagatggccg aagtggagca gaagaagaag cgcaccttcc gcaagttcac 60 ctaccgtggc gtagacctcg accaactgct cgacatgtcc tatgagcaac tgatgcagct 120 gtacagcgcc cgccagagga ggcgcctgaa ccgtggtctt cggcggaagc aacactcact 180 gctcaagcgc ttgagaaagg ccaaaaagga ggcaccaccc atggagaagc ctgaggtggt 240 gaagacgcac ctgagggaca tgatcatcct gccggagatg gtgggtagca tggtgggcgt 300 gtacmacggc aagaccttca accaggtgga gatcaaacca gagatgatcs gccactacct 360 gggcgagttc tccatcacct acaaacccgt gaagcac 397 333 405 DNA Murine 333 gaattctgga gaagtgggag gtgtactgta cggggaggga ccaggggaag aagagggggg 60 tggaaagtaa gaagggagga aaggcaggag ggggagagag agatgttact gctttctttt 120 cagcacatat aaaacaaagg actaaagaaa cgcatattta aaatccagtt tctatattca 180 cacctaattc acttccaaac ctacttgtaa aaatccatct tcagcaaatg aatttgttgg 240 gaaaatggcc aggcatccat acacagaaag gttctccatc accataaatt aactcatggt 300 atgctgaatt aattgttgaa aattactaga aaatatgttc acaaacctgg caaattcaga 360 ctatgtcaca cacaaatact cctttctttc tccctcctcc tccct 405 334 300 DNA Murine 334 gaattcggaa tgttaccgca ccgcatgctc tccctgcagc ctttcttgca cactggcatg 60 ctggtctagg agccgctatc tatcctctcc acaatgcctg chcgcctcct cmcvcagttg 120 acaagccaag ccgccactag cttcatcacc aachcgctct cctccaccat cctggaaccc 180 tttcccagct tcaccaccac atccgtatmm ctccttcttc ctagcttcct ccaccgaacc 240 gcactctttc ctgggctatc ttcaccatgc actgctgctg chgctcctca gtccttccta 300 335 357 DNA Murine 335 gaattcgttg gcgaatcatc atctcttcct ctcgtctacg ccgttcctcc tcttgcctca 60 actgcatttc tttacgtttc tgcatttctt gactgtgaag ttcctccatg cccttaattc 120 ttcctggcgt ctcatcagat cttkgcgcaa aagatttgct tggtgttcat ggtaagcatc 180 ttccatttca ctttccaatt tgtctttagc atccttcatg tttttttcaa cttgttccct 240 ttgctgtttt tccatttcat ccaaggactt ccatygttga gaatattcac tcaaatgtgc 300 catgctgagc aaaacgaggt ggtgtttctc tctccttttg atacattgga ttcttct 357 336 427 DNA Murine 336 gaattcttcc catgcacatg caactctatg gagacgctct cccttgcacc ttcgtaggct 60 ctgtgtgtcc tcaggctgcg tggtgagcgt ctcgtaccta cagagccatt tcacagctcc 120 acaaactcag tttaaaacag cagcaccgct tctactctat gcttcggttc aagtgaggaa 180 gtgaggcagg tacaattgcg tcattcacac ggctctagtc aggtagctgg agcagagagg 240 atggagaaca ggctcatggg catctctctg tgctgagtat cctgggctct tttccacaag 300 gtctctccca taacaaaatg agccctggac agctacaggt gtcatacccc agtgccgcac 360 tccaacaact tcacagcttg ctagaactcm gaaatcaata aatcagaatt cagagcctca 420 ttcctct 427 337 424 DNA Murine 337 gaattctttc tcagctgtaa ctgatggctg cgtctgaagc ctttactcta atggtgctta 60 tgtctgtgtg ggttgcaccc attctatagg gctaggtgga attgacgttt gttcttacgg 120 tgttttgtct acttcattgt actgtgacat gcttgaataa gggagggagc gaatggatta 180 cttacctgtc aggatgccaa gagcacctgt gtggtctgtc agcctggacc tcagtgagct 240 gcggtaagca ggttggcaac ctcaggctca acccatctgt gaggtcaatg catcttggaa 300 aacagaaagt gacctggcag catattcctt atttgtagta ctgttttgtg tttagtttgt 360 tgttgtcctt tgagacaggg tctctttaag tagccctgdc ctsgccgtga aatccacaga 420 gaac 424 338 389 DNA Murine 338 gaattccaca attatctcat caataattac cctatttatc ttatttcaac taaaagtctc 60 atcacaaaca ttcccactgg caccttcacc aaaatcacta acaaccataa aagtaaaaac 120 cccttgagaa ttaaaatgaa cgaaaatcta tttcctcatt cattacccca acaataatag 180 gattcccaat cgttgtagcc atcattatat ttccttcaat cctattccca tcctcaaawg 240 cctaatcaac aaccgtctcc attctttcca acactgacta gttaaactta ttatcaaaca 300 aataatgcta atccacacac caaaaggacg aacatgawch ctaathattg tttccctaat 360 catatttatt ggatcaacaa atctcctag 389 339 388 DNA Murine 339 gaattctttt tggcttctta ggaggtataa agttctttcc aaacactgct tctcttcttt 60 ctaaatctgc aggatttcca cttaaacctt cattgggaga tgttttcaac ttggtgcaaa 120 tgccatagac atctccatag ctctcctgta ttttccgtaa tgcatccgtg gatctgagct 180 ccatgagagc tcgcagctct gtaagcgtaa ttccaaagtc tccatcatgg ttagcttctt 240 tcaaagagtt cttcacaccg ctatacgcaa ctgagttgtt ggccatgtcg cccatcacaa 300 ggataattta ttaggaggaa aatgtttccc agaagaatga aattttcacc ttgaatgtag 360 atttccaagc tgaaaatctt ttaaatat 388 340 230 DNA Murine 340 gaattcccca agcttgtgca gaggccagga ccaccttgtt ttcatgctct tgactgctgg 60 gagcagtgga agagctaaga acagagtagg ggcccsaggg ctggatctag cccagcccag 120 ggscaaaaag gaaaagaggg gagtdctccc agctggtttt ggcttggtga aggcytgggc 180 tgggagttct ygagaggcct cctygctatc ttagaccacy ggktctttta 230 341 200 DNA Murine 341 gaattcacat atgcaaagag actgaatgtg gatcctttga ctttctcttg ctcccgatct 60 ctgtgcctcc tagtagagca cccgcccact gggcagccca ggaaagagct ggagacatca 120 gcccagtgga cttctaggaa gttgaaaaag caaaataaaa cattttcaga gagcgtttcc 180 caaawchgcg agcattctca 200 342 350 DNA Murine 342 gaattcccct acatcaaaaa ttatttaagt tgaccaagat aaaaaactgt ctctaaaagc 60 ttatatacat tagaagtagc aaaaataata ataaaggaag agattagaaa acagccatca 120 aattcagaca tctacaagaa ttctccaaca tctgctctct tatctcggca tttgcttcga 180 gcttttgttc gagctttgaa agctgcagag ttatataaat gcctttcaaa acgagaaatc 240 ttcatggttt taagtgttgc agcatcaagc atcacagggg ggtccaagct caaatacttt 300 tcgaggratt mmwtttgtct gcaagtggta ctgcatccct gatccmagaa 350 343 376 DNA Murine 343 gaattcgcgg ccgctttttt tttttttttt tttttttttt tttttttttt tttttactgt 60 taaaggattt attgcagtaa tacaacaaag gtttagaaaa catctgtgtg atcaacctga 120 cctggaagtt tcagtcgcag caagggggtt ctgacgttgc agctttccca atgcacacct 180 gaaccccacc caatgctgac ccccatacca tggtaagtta catttcttgg ttctacgtaa 240 gaccatgaac agcccgtgtg gtgcctctga gtgtctatta gtattacctt gttccaagaa 300 atcattttta aatggaaaac atgatcaact tctatggctt tcggtttaaa aaaaaaaaaa 360 caaawcacca gcttca 376 344 481 DNA Murine 344 gaattcgtcg tttttgctgt caccagcaac attgcctcgt ctaacatctt tgaccgacac 60 gttctttaca ttgaagccca cattgtcccc aggaagagct tcactcaaag cttcatggtg 120 catttcaaca gacttgactt cagttgttac attgactgga gcaaaggtaa ccaccatgcc 180 aggcttgaga acaccagtct ccactcggcc cacagggaca gtgccaatgc ctccaatttt 240 atagacatcc tggaggggca gtcgcagggg cttgtcagtt ggacgagttg gtggtaggat 300 acaatccaaa gcttccagca gcgtggtgcc actggcactg ccatctttgc gggactttcc 360 atcccttgaa ccaaggcata ttagcacttg gctccagcat gttgtcacca ttccaaccag 420 aaattggcac aaatgctact gtgtcagggt tgtagccaat tttcttaatg taggtgctga 480 c 481 345 507 DNA Murine 345 gaattctttt aactgtatta ctgaatacct gaggtagttg agtaaaaatg cacgtttaat 60 accctgccaa cagcggctgg cacttccctt aggttatcca tgttagtgtt agagaaacag 120 gagacaacag ctcttctatt ctaatggctt aatgttgtgt tcctctgaca attctacttt 180 gatccaattt caacaattgg acttaggaac aatctagttt taaatttatt tgataaattt 240 agtgaatgta ccatttatdc caatttctgg cattatagag ggatattaag aaaaattagc 300 acgtttgtta tactttgata tcacaaggga agtgcagagt tctctttcct tacccccact 360 tttgtttgtt tggggttttt gtttttgttt ttattttagc tgttttttgt gcatgataca 420 agttwagatg ccctggatgt ttgattttgg atgacatgct atgtycttgt cagtggtggt 480 tcatttgcag taaatygatt gaggaca 507 346 429 DNA Murine 346 gaattctgga tattaatgag agactacggg tatcgagata tcaagagtag gaattaaatc 60 atactcccaa taagagaaca tattcccaca acagaaatac tcattcccct aattgcaagg 120 aagattttaa ggcagtgagt ctcaaactgt aatcttacca ccagcagctg taatgctgca 180 aaaattctca ggttctaccc agacctacta gatcagybct gggggttagc taggcagcct 240 gtgtgctaac aagtctctct ggggactcag gtacacaatg aagtttaaga aaagtgcttt 300 tcaggctggg gatacagttc hgttgggaga atcttgccta atatgttcaa ggccctgagt 360 ttggttatca gcattacata agtgtgtgtt tgtacatgcc tgtcctcttt gggaggtagg 420 agataaagg 429 347 274 DNA Murine 347 ghcccccggc tagagcggct tttttttttt ttttttttgt tttttgaggc agggtttctc 60 tgtatagccc tggctatcct ggaactcact ctgtagacca ggctggcctc gaactcagaa 120 atctgcctgc ctcgcctccc cagtctggga ttaaaggcgt gcccaccact gcccagcttt 180 tttttttttt tttaatcctt tttatttttt ttaatagcta agtggtttga ctggttttca 240 gtggtagacc acgtggaaat gagaatattt atca 274 348 287 DNA Murine 348 gaattccccg gctcgagcgg ccgctttttt tttttttttt tttcagaaag ccagtttatt 60 tctaagactt tgtcataaaa cttttagcgg gtaccaatag ttacctgcca tactcgcacc 120 aagttgtctg tatagccagc aaacagagtc tkgccatcag cagaccatgc caaagaggta 180 cactggggtg gctctgcctt kctgctggtg ctgataactt cttcttcaat tcatctacaa 240 tgatcttgcc ctccaagtyc cagatcttga tgctgvgcca tggcagc 287 349 403 DNA Murine 349 gaattcgctc tccttccctc ggaacaacat tagctacctg gtgctctcca tgatcagcat 60 ggggctcttc tccatcgctc ccctcattta tggcagcatg gagatgttcc ctcggcacag 120 caactctacc gccatggcaa ggcctatcgc ttcctgtttg gtttttctgc tgtctctgtc 180 atgtacctgg tgttggtact ggcagtccaa gttcatgcct ggcaactgta ctacagcaaa 240 aaactcttag actcttggtt caccagcaca caggagaaga aacbgaaatg aagcctgctt 300 gataaactgc tctcgagggg taaaacctag gbctcccatt gagcagcgtk aagggagchg 360 tccagactct ccatcgattg tvgcatctgt gatgttkgvc acc 403 350 231 DNA Murine 350 gaattcggtt accatcgtta agccaatcgt ttatggcaat gttgccaggt actttggaaa 60 gaagagagaa gaagacgggc acactcacca gtggactgtg tacgtgaagc cctacagaaa 120 csaggatatg tcagcatatg tgaagaagat ccagtttaaa ttacacgaaa gctacggcaa 180 tcctctaaga gtcgtcacca agcctccata tgaaatcaca gaaacargat g 231 351 321 DNA Murine 351 gaattcggcc atctggctta ggtgccttac actggttgca ttcatttctc caagagaagt 60 tcatgttctc acatgtagga ttaggacact tccagtctcc agctcgttgc tgtcctccac 120 ctccaccacc tccactgggg aatcctcccc ggccaccacc accactgcca cctcctccat 180 agcctccacg gcccatgggt cctcctcgvc ctcggcctcm vcgaccattt ccaccacccc 240 gattgaagtc agctcggcgg gtagcaaatg aaaactttaa taggattccc agagaattct 300 ttaccatcaa aacmagtcga t 321 352 319 DNA Murine 352 gaattcggcg gcgtttattt ggagcaaatt cagctcccgg agctggacgg ttgaatgcag 60 gaggagttcc accaattgct ccaattcctt ccattgttgc agcttggcca aaacgttcag 120 ttgttggtgg ggtcaatcca agggttccat ctggcatcat agtggcaggt cctggaggag 180 ctggagtacc aggtggcaca ggagcagggg gcatcgcgcc tctattgttt atgcccatag 240 cacctcccat agccatttgg cccatccgta tctcttvttc tctcgcatca gggaaggttc 300 ccttgaatcc ttccwgcgt 319 353 286 DNA Murine 353 gaattcttcc atatttgtat catgtagctg tgcttttagc ttttcatttt cagctaaaat 60 ttgttcataa agctttttga agtcagttga gtcatccttt tctagcctgc tactgtaagg 120 ttttctgtct tctaagtaac tgtatgaagc agagcgaccc agcaaggaat cataccgatc 180 acttgatgat gtggaactgc tgtcatacct ggaaacagaa tccgtctaga aagtaaaaaa 240 aaaaaaaaat ttckgscckc hcgadcgggg aattccacca cactgg 286 354 379 DNA Murine 354 gaattcccag tttctggctg ttataaataa ggctgctatg aacatactgg agaatgtgtc 60 cttattgcaa gttgaaacat cttctgggta tttgtccagg agaggaattk ctggatcttc 120 tggtggtgtt ttttttccaa ttttctgaag aactgccagg ctgatttcca gagtgcttgt 180 attagcttgc aatcccacca acaatggagt gtttcttttt ctccacatcc tcgccagcat 240 ctgctctcac ctgagttttt gahcttagac attatgacyg gtgtgaggtg gaatctcagg 300 gttgttttaa hgtgcattyc cytgataatt aaggatgttg acmtttcagg tgcttctcag 360 ccattcagta ttcgtcagg 379 355 319 DNA Murine 355 gaattcgaca aacagtaaga cttgactgga atatctagtt acagaatatc ccagggaatt 60 ctttggtctt atcattttaa ggaaaaagaa aagcaacggc aagcagaatt acaggagaah 120 gaaatcgcag aaaaaaagtt taaagaatgg ttggaaaatg caaaaaataa acctcgtycg 180 ctgcaaagag ctatggttac tccagtggaa acttacaggt tggattttac gtctgtgctt 240 acataaatat ggtttgcaga agcaaatgat atatatagaa atgtataaaa gtaatttttc 300 tttgaaatta ttattttct 319 356 104 DNA Murine 356 gcgctaggcg agcgcgcctg cctgaagctg cgcattcccg atcagaaatg acccagtcgt 60 cgtcctctcg gcaccgaatc gtatgattct ccsccagcat gctt 104 357 87 DNA Murine 357 gcggtaggcg agcgcgcctg ccctgaagct gcgcattccc gatcagaaat acccagtcgt 60 cgthtctctc cccgaatcgt atattct 87 358 260 DNA Murine 358 gaattccgct gcctcaagct ggcttaagtc ctgctgagat tcagcaacta tggaaagaag 60 tgactggagt ccatagtatg gaagacaacg gcatcaagca tggagggcta gacctcacga 120 ctaacaattc ctcctcgact acctcctcca ccacgtccaa agcatctacc acccatcaca 180 catcattcca tagtgaacgg acagtcttca gttctgaatg caaggcggga cagctcatca 240 catgaggaga ctggggcctc 260 359 163 DNA Murine 359 gaattccgag gccagcgccg cggtggagaa gctagtttcc ggcgtgcggc aggccgccga 60 cttcgccgag cagtttcgtt cctactcgga gagcgagaag caatggaaag mgcgcatgga 120 gttcatcctg ccacctgcct gactaccgag acccacccga cgg 163 360 552 DNA Murine 360 gaattcgtac agtcaccaaa gtcacatttc agaggaaatc ttaatagatc ttctcacagc 60 caaaaatgca agaagcacac attttatagt tttaagtttg tatctcagag cctcagtcca 120 tacagaacaa agtcagccca acaaaatcag ttcaaggaaa acaaaagtta atttgcttgg 180 gcttcctagc taacacttgg ctattttccc actcaggtgg aggagtgtgt aattctgcca 240 gtgcccggga gctgagcacc caggctaaaa cacacaaaaa aacacaagtt aggtcctggt 300 gctgagaaag ttacagttag agcggaggct gctgacagcc tggagttcct ggaatgatca 360 caactccagc agcacaacct tgacttacaa ttgrcagctc tgctctactc tggggtctga 420 aaaccccaga gaggcgcaaa gctgactcta agaggcaagg tctgtcttgc tgttgttcta 480 ttgccacgaa gagacaccat gaccaaggca actttgaaag catttaattt gggggktcat 540 ggatccaagg gg 552 361 434 DNA Murine 361 gaattcctgg aactcactct gtagatgaag actgtagcag aactcagaga cccacctgcc 60 tctgcctctc aagtactggg actaaaggca tgcagcacta ttgcactgct gagttttgtt 120 ttctttttct ttcttttttt ttttttttgg tttttcaaga cagggtttct ctttatagcc 180 ctggctgtcc tggaactcac tttgtagacc aggctggcct cgaactcaga aatacgcctg 240 cctctgcctc tgcctcccga gtgctgggat taaaggcatt cgccaccacg cccggccttc 300 tttttttaag attaaaagta aattactttt attaatttaa agttatgtgt gtgtttttct 360 ctaggtatgt acataagaat gcagatgccc acacaggtca gaggcatcag atcctcctgg 420 agttaawgct acaa 434 362 426 DNA Murine 362 gaattctgag tgagctgacc caaggcccat tgggctcaga ccttgctgaa tatgcttggt 60 gacacctaaa cctgcgcgct gttctcattt tggaactgtg tctggctttt gcttttcctt 120 ccgcacagga aactatcatg aaattccttc ctttgctttg gtgccaaagc ttcatctcat 180 ccatttcttc agcagccatt tcctgagtgt ctgcactgta ctgggcctgg ttaaaggcca 240 gggaaaaagc agatgttgga aaagaagcct gcatacttcc gtagaatgta agatgtaact 300 cagagttgag aaaagggagg ggtgacattt gtaacttttt cccttgctgt acagtctaca 360 ataaattata ctacataaaa ttctttaaca gtattcatta atgtagctga cccattagga 420 tggaaa 426 363 452 DNA Murine 363 gaattcgctc caaccattct ggtcaggaaa gagtgtgagc atgcttcctg acaactgcta 60 gaaaaactgt gagttgagta cactgctcct ctttattatg gcccaaacct ctgaccttcg 120 gtttcttttg caaggaactg aagaaagagc tgagaccttt cttattctgt ggaatgtcag 180 aggaagatca catgacaaag gctgaacact tttagctttg ttgtgtacta agtccagtgt 240 atcaaataag aaaataactt actctggctg ctgtagggtg ggagatgagt atcatggatt 300 ctagacaaag tgaccaactc tctctcatat acaaavcaca ctctgggggr ctcccaaagc 360 gatcttcctg aaagctagac ttctgttaag taactccaac aacacagtct cttbggtgaa 420 tatgtaagtt tttttaaaat atttttaaga ac 452 364 380 DNA Murine 364 gaattcctgc catttccagg agattgctga gcatcttcac aaaaaccaga actttccaag 60 tgctgagtag gatcaccacc taaataatac tcttcttgtc caaattgctc catagagtca 120 cagtacactt cactatctga atcacttgtt aaatggtgta ttcctgaagc atcttcactg 180 ggatcttcat ttctatcttg gtgagcacag acaatggtgt tctgtctgct gagagctctc 240 atctccaggc ttttcatctt cacvcttctg gtgcccggga agaatcagta tgaatgtcac 300 tctgtatatc ctgaacaaag ctacctttat agccattgta acaatgattt ccaaattctt 360 atctctgatt ycytcagctt 380 365 308 DNA Murine 365 gaattcccgg ccgtccctct taatcatggc ctcagttccg aaaaccaacw aaatagaacy 60 gcggtcctat tccattattc ctagctgcgg tatccaggcg gctcgggcct gctttgaaca 120 ctctaatttt ttcaaagtaa wckcttcggg ccccgcggga cactcagcta agagcatcga 180 gggggckccg agaggcaagg ggcggggack gkcggtgact cgcctykckg hkgaccgcyc 240 kctccccaag atccaactac gagcttttta actgcagcaa ctttaatata cctattggwg 300 ctggaatt 308 366 479 DNA Murine 366 gaattcagac tttgtcataa aacttttagc gggtaccaat agttacctgc catactcgca 60 ccaagttgtc tgtatagcca gcaaacagag tctggccatc agcagaccat gccaaagagg 120 tacactgggg tggctctgcc ttgctgctgg tgctgataac ttcttgcttc aattcatcta 180 caatgatctt gccctccaag tcccagatct tgatgctggg ccagtggcag cgcagagcca 240 gtagcggttg gggctgaagc acaaggcatt gatgatgtcc ccaccatcta aagtgtagag 300 gtgcttgcct tcattgagat cccacagcat agcctggcca tccttgcctc cagaagcaca 360 gagggatcca tctggagaga cagtcactgt gttcaggtag ccagtktkgg ccaatgttgg 420 ttgggtcttt agcttgcagt tagccagatt ccacaccttg accagcttkk tcccatccg 479 367 475 DNA Murine 367 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg 60 tcaagcatga caaagttgca gccgaataca gtgatccgtg ccgcccwgga cctgttgaac 120 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag 180 ccggcgcttt actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc 240 atgctggcgg agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt 300 ctgatcggga atcccgcagc ttcaggcagg cgctgctcgc ctaccgccag cacactggcg 360 gcctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat tacaattcac 420 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaa 475 368 543 DNA Murine 368 gaattcatta actgtgctgt gataggatgt agggggtgaa gtaagagggt aagcgcctga 60 tgtccctggc tgctttggaa atggctgttg ctgaggtggc tggagctgtg atattaaaga 120 gtccatcatg tcacctccta taggagaagg agggttatca tcctcattta cagatcttct 180 ccgagcatct tgattgctat caacaaacat gttcaggaaa gtctttaatc ctggtgcagg 240 atagaagcct tcaactaact tgctgttatc aaaaagacta taggcaccgt cccgtattgc 300 cacgacgcct cgactacggc agtatatgtc aatgcagtac atgttcctga aggccagtct 360 gatgtgggtg gatgattgtg gtaaaatgga gaaacccygg taggcsgtgt tagttctctg 420 gtcaagccca acattggaac agtagggagt ttgttgatag catttaatgg tgcctgagta 480 tcaaacaaaa cctgtaataa ctgaccacat ttggtgtttt gtttgaacat ttcttgaagt 540 tga 543 369 409 DNA Murine 369 gaattcggcg gaggcggcgg cgggcgaggc gggcgcgagc gagcgggacc cagacgcggg 60 ccgcgccgcc ggcggctgcg ggttctgtcg ggccatctgc tgggccggcc ccaggaggct 120 ccgagtacca atgagtgcaa agcgcggaga gccgcgtcgg cggccggggc gtcgcccgcc 180 gctactcctg ccgcaccaga gtcgggcacc atccccaaga agcggcaaga agttatgaaa 240 tggaatggat ggggctacaa tgattccaag ttcttattga ataagaaggg ccaggttgag 300 ctgactggga aaaggtaccc acttagtggc ttggttttac caactttgag agactggatc 360 caaaacaccc ttggagtagt ctggagcata aaactacctc taaaacatc 409 370 139 DNA Murine 370 gaattcgaac atttgctcag gtatgaggca gggtgagaaa gctgggtgag cctgcatcta 60 caaactgagt gaattatttt chhtctgtgt gtgaatgtca gcatgacacc ctgagtagaa 120 sccagaccct gtcccctat 139 371 382 DNA Murine 371 gaattcctca aatatctata taataattta caaccgttgt tgtggagata ggatctcact 60 acacagtgca cgatgccctc aaaattatgt agctgaggct agtcttagcc ttccaggcgc 120 tggggttaca gatatgtgct attacaacca ggcttggctt atactcttag tatgcaaaca 180 tagtcttcat atttttatat acctaatgca tgcctattat acaatacaca aaatcatgca 240 aagctatcac aaaattctgt agtagaaaca atttgattta tgccaactgt atgtctcaca 300 taactcaatt ccttctttta agaatgaagt cttcaatttc aagtgataat tctattaaaa 360 ctagaatcaa cacagtaaaa at 382 372 319 DNA Murine 372 gaattcctgc tataataacc taagctatta agtcacaaca gttttagctt ttctttttat 60 aagagtttaa gattttattt atttttattt tatgtgtata agtatttgtc tgtgcatcat 120 gtacatgcct ggtgcccata aaggcaagaa ggggacactg gaattacatc cctgtaattg 180 aacagggtcc tctgtaagag cagacagtgc ttataattgt gaagtcctat ctgttagvcc 240 ccagtttttg gttttcaaaa ggggtaactc taaaaaatat tataraacag aacatgctca 300 aaataaaatg ttggcaaaa 319 373 261 DNA Murine 373 gaattcgatg tttcgtcagg agagatgagg taacaaacta ttgataacaa catagccata 60 agagaccaat actgacttca agactcaaaa gaacacagac cctaaaatca cagctttcag 120 gcagtgtgtt tctagaccac ggggcaactg tacmgcacaa agcagcatgt gacaagaaac 180 atcattgaca aggcagttct catgggggat ggagcaggct agtgggggtc ggggtcactg 240 cyggaaamct tcagaccgca t 261 374 557 DNA Murine 374 gaattcgcgt cggacctgcg gagcccagga tggtgttgct cgagagcgag cagttcctga 60 cggagctgac caggctcttc cagaagtgcc gctcgtcggg cagcgtgttc atcaccctca 120 agaaatatga cggtcgcacc aaacctatcc cgaggaagag ttctgtggag ggcctcgagc 180 ctgcagaaaa caagtgtctg ttgagagcca cggatgggaa aaggaagatc agcaccgtgg 240 tgagctccaa agaagtgaac aagtttcaga tggcctattc aaatctactg agagccaaca 300 tggacgggct gaagaagagg gacaagaaga acaagagtaa gaagagcaaa ccagcacagt 360 gacaggcgtt ggctgctacc aaccagctgc acaagtgcat ttttcctctg tttgctgctt 420 tcagcacctc tgtatgtaac tgtttccacg gaagggtcct ttaagagaga aggactggga 480 tgggcatggg ctagttgtbg taagacgcca kttttsattg tgcygtgtgg gctggatatt 540 cttagattcc agccgta 557 375 195 DNA Murine 375 gaattccatt ggcaatttct ttttccaatt ccataacttt attcatttcc aaagagagct 60 ggttttcatc aataggcaaa ctttgttcct gacgaatcag tctggccaca gaaatcataa 120 aatccacata tgctgtgcaa gcctctttat atawtccagt gcactcagac gcatgcccyc 180 amgcatagtt acaac 195 376 288 DNA Murine 376 gaattccttg agaattaaaa tgaacgaaaa tctatttscc tcattcatta ccccaacaat 60 aataggattc ccaatcgttg tagccatcat tatatttcct tcaatcctat tcccatcctc 120 aaaacgccta atcaacaacc gtctccattc tttccaacac tgactagtta aacttattat 180 caaacaaata atgctaatcc acacaccaaa agggacgaac atgaacccta ataattgttt 240 ccctaatcat atttattgga tcaacaaatc tcctaggcct tttaccac 288 377 197 DNA Murine 377 gaattccttg tgtgcctggt cagctccata cacccagcaa ttcacctgta agatctgtcc 60 tgctttggag gccgtggagt ggagtcttcc tttttcagga tgaaagaagt tggcttctcc 120 taaagacaac agtctcagac aggtctcaag attccctgtt ctcacacttg aatgggtcat 180 actgagatct ttccgtc 197 378 229 DNA Murine 378 gaattctgga gttccgcagc ttgacccaca catttgccag aggtgagaaa gtggccgctg 60 aggtcttgct gcttccctga ggccggttcc ttcacgagag agcagtagtc gttctcaagg 120 tggggagcga aggggctgct ggccccgctg cggchcgcca caggacagac catcggaaga 180 gctgtyvgcc tcagagttaa gggatggctt cttggggccc aggcgggag 229 379 57 DNA Murine 379 gaattcatgg aactactcca tcaataggca aagtggcatt gatttttatc tcdattt 57 380 356 DNA Murine 380 gaattcccaa aagtgaaata agatgtccac attaaaaaaa taaagcctac aaaaaagttc 60 tggagctaaa aaaattattc atatggcaca atgtgatctc caaggtccaa aatattgaaa 120 tgagatccgt gtaagcatcc tgtctgcttt tcaatgcagc actaacttta ctgaggtgaa 180 atcacaattt agttcttcag tcaacaagtg gacacaaatg tttttctaca gttattaaaa 240 acaggagatc aagttgaatg tdccgaaatg atttcttcag ttggatattt tagtatcttg 300 aagaaaatta gtdaagggat acttgtcgtt tccatagcyt gatagaccaa aacaaa 356 381 371 DNA Murine 381 gaattcgcac gcaagcccta tcataccaca ggaaacagag cacaagagaa gtgtacagtg 60 gagtgggcat scgtaaaaag atggtgtttc caagcagaag tatatgcaaa grctttgcta 120 aacagaaact gaacagatag cttataccat tagatcagat tttgaagggt tttaggatgc 180 atggagatgg gccactaggg ttgactatga ccgaggtcag gtattatgtg tttacttaag 240 attcctttct dscgatgaga atgcattctg actccagcat gcaccaggtg cgcttdctdc 300 ccagadctgg gattgccaat tccaagtgtk cctagccttg aggattgacc ttggscctga 360 gcatagcctg t 371 382 323 DNA Murine 382 gaattcwcgc tcwchcttcc tcagthcttt caaagtcaca ggaacctggc aatttccctt 60 ttcattcccc ctcccacttc cctggtaagt hcctctcgga atatcacaag agtttccaga 120 hctggttcgg atcacctttc ctgtaattaa ttaattatga gaagaaacag acagtacaat 180 agatctgata agatgtagca ttcttgttaa gattaaacaa tacatttatc maayhgtatc 240 agaacaaatt aacataatat ttaatcttat mmvcaccaat aaccacagga attgttattt 300 ccaardggag agtcttgtta gaa 323 383 379 DNA Murine 383 gaattctgtt tatgtagcat ataaataata taaaattaaa cataaagaac ttagtatttt 60 attgtaagtg aaaaaaataa aactagaatt gtcatattaa tggtcctgca tatcaaataa 120 ttttcaccaa gtctctgtaa tacatactaa cagcattaga cacagggaaa caatcaagat 180 gatcaaattc ataacaaaaa actgtattgc taacattgta acattttata agagttaatt 240 gaatagtgac caaagttctc ccttaaccct tccatctgat gactgtgaga ttgtttttta 300 agtttgctgt aaaagaagac ttgccttggc cwmctatacc tycaaccaat ctatagaatt 360 cagaggacca ggagggtac 379 384 63 DNA Murine 384 gaattccaac agttttgaaa gtaattaaga gaaatcacaa acagttaatt ctgtcctcca 60 aat 63 385 193 DNA Murine 385 gaattctttt aatacaagtt attgtcgaag aaatcactgg agggagaaaa aaaaaatctt 60 cttcawccca caacacttaa aaagtaacac atgaaaggag aaatctggta acaagcagga 120 tagacttcat tctagtaaaa agaaataatg tttcaaaaca caatctaaag caggcttcca 180 ttagcaaaga aat 193 386 252 DNA Murine 386 gaattcgacg gccgtttttt tttttttttt tttttttttc ttttcttttc ttttctcttc 60 tcttctctts tcttctcttc tctttctttc tttctttctt tctttctttt ttggtttttt 120 tcgagacagg gtttctttgt atagcctggc tgtctggact cactctgtag acaggbggct 180 caaactcaga aatctgctgc tctgctgttg agtgctggga taaaggcgtg ccacacactc 240 ggctgagayc tg 252 387 103 DNA Murine 387 gaattcggac aacaactccc acaagaagaa catcttcgag aaacccttca ggctcgctac 60 gtgcgtgtcc ttccagtstc ctggcataac cgcatcaccc tgc 103 388 153 DNA Murine 388 gaattccaga tcccattaca gatggttgtg agccaccatg tggttgttgg aaattgaact 60 caggacctct ggaagagcag tcagtgctct taaccatctc cccagcccat gtcttacatg 120 tttrtttaaa tgaggaacga tagtgtggts att 153 389 337 DNA Murine 389 gcgttaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca 60 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag 120 tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc 180 caaccgttcg ccagtttgcg tgtygtcaga ccgtctcccg acctcgttca acaggtccag 240 ggcbgbacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat 300 aaacataata tgtccaccaa cttatcagtg ataaaga 337 390 281 DNA Murine 390 gaattctttt tttttttttt tttaaagact tatttattat taaatataag gacactgtaa 60 ctgtctttag acacaccaga agagggtgtc agatctcatt accaatggtt gtgagccacc 120 atgtggttgc tgggatttga actcagtatc ttcagaagag cagtcagtgc tcttaaccac 180 tgagccaact ctccagcccc ccaaaagaca gccagcatta cactgagctt agagccagcc 240 tggttatgta tcaagtctgt gtctcaaaat gaaaagtgaa a 281 391 262 DNA Murine 391 gaattctttc aactccaatc tctgactttr ctcattgctt ctcagcttca aaatgcaagc 60 acagactaca gctaactgag aactggctcc actcaggggc tatggcgcag gagccctgac 120 gcatgcctcc gcvgctgccc caggctctta ccagcaggta gtgctggcgg tgttcagctg 180 ctgcctcatg ctgggcaggc tctkctgcct gtgcaacatg tctgacggaa gttaaggcct 240 ccagtctaac aaggtttctc ac 262 392 399 DNA Murine 392 gaattcgttt tttttaatgg ctttttgtaa catcgctgca ggaagcgggt ttctttgttt 60 tcttttcttt ctaagagaag gtatctccct ggtgcaatag ctcggcaccg ccggcggggg 120 cctctcgaca caccccagcc ctgggctcct ctggcctcca aatcattcag gatggtgagg 180 gaggatggga aggagggggg agggggacag gtaaatcgca tctgcgccca cttctctctc 240 tacctccttt tggagaacca gccagcctgg accactttct ccatcttagg acaacttgag 300 gctccttgct ctcatctgtg cttcagagaa ttcctttccc tcchgggttc tgtctggttc 360 tcagcagggt tcccaggcca ctgtgcagtg gcatctagc 399 393 632 DNA Murine 393 gaattcgggg gagaaagaga gggagggaga aagagagaga gagagagaga gagatcttgt 60 tctcctggca caatattaac tgtttataat taagctaaaa acttgttctg gtattttatg 120 acatcaggga aattctttcc tctctaggca gattgccaaa aacaactaga agctaaatgc 180 ctgtgccttc tgcttctacg acacaccact ccgtcttgtt cagtttcaac tagcgtcgct 240 ctaaaaggac aaaaaacttc ttgtttttct aaataaaaca taaatggccc agaatttgaa 300 ttgccgatct taaaatttta agtgactgaa gattctatta attctggcaa taaaatcatt 360 aaaaacaaaa caggttgcat aagactttta aacaattcat tcacaggcat gagaatttaa 420 ggtttctttt aaaatataaa atgctaaaac aataagtcta acaggagaat atgaataata 480 cmatattcta agaaaaaaac ccacaaagac aaacatgaca tttcattcat agctcattca 540 aataaaccaa ggattaaacc ttagttttaa cctgttaatt ttcctttttr ytttagtatg 600 tctgatgtcd catgtacgrt arccagaagg cc 632 394 376 DNA Murine 394 gaattcaccg gctcgacggc cgcttttttt tttttttttt tacataaaaa gactttattt 60 gcaggggagc aggaatttaa tcaaacaagc caaatcccat gtcgtcatcc gactcctcgg 120 actcctcctt cttctcatct ttcttctcct ctgctgcagc gggggcagaa ccagcagcag 180 gtgctgcaga gccaggggca gcagaaacag ccacagcccc accagcaggc acactggcca 240 gcttgccaac accctgacga tgacatcctc aatgttcttt ccattcagct cactgatgac 300 cttgttgagc cgatcatcgt ccgcttcgat gcccacctgt ctagtatttt cttgatgtct 360 ttggcactag gagagg 376 395 348 DNA Murine 395 gaattcrgcc gcttttdrtt tttcattacg gtaaacagga atatattcar atgctaatrc 60 ctcctttgac cagaaatgga acatgctgaa ggatgaagac aaggatcttt dvcctttgct 120 tgaggtacch garctggtga cgttcagtta ttctaacagt gtcattcagt cacagtcatg 180 gcctgaacca gaatgtgtgt gtgtggtaaa aatatctgtc ttcacaacag tttctggtgc 240 rttgtagaat agcacataac tgctttctrc agtttgtdct ttgacagtat aatgtatgtt 300 ggtcatattt aacccaaatc atctctccct ctaacattgc aacacccc 348 396 468 DNA Murine 396 gaattcgcac ttttgatgtg tcaatcctca ctattgagga tggaattttt gaggtcaaat 60 caacagctgg agacacccac ttaggtggag agattttgac aaccgaatgg tcaatcattt 120 cattgctgag ttcaagcgaa agcacaagaa agacatcagt gagaacaaga gagctgtccg 180 ccgtctccgc acggcctgcg agcggccaag cgcaccctct cctccagcac ccaggccagt 240 attgagattg attctctcta tgagggaatt gacttctata cctccattac ccgggctcga 300 tttgaggagt tgaatgctga cctgttccgt ggcacactgg accctgtaga gaaggccctt 360 cgagatgcca agctggacaa gtcacagatc catgatattg tcttggtggg tggttctacc 420 agaatyccca agattcaaaa cttctgcaag acttcttcaa tggaaaag 468 397 381 DNA Murine 397 gaattcgtct tcaacggctt ctgtaaatct cggtgacccc acaaggcgta ctgaaggaga 60 ttacttatcg tacagagagt tacattcaat gggaagaact ccagtcatgt caggatcaca 120 gagacctctt tctgcacgag cgtacagcat cgatggccca aatacatcca ggcctcagag 180 tgcccgtccc tctattaatg aaataccaga gagaactatg tcagttagtg atttcaatta 240 ctcacggact agtccttcaa aaagaccaaa tacaagggtc gggtctgaac attctctgtt 300 agatcctcca ggaaaaagca aggttcctca tgactggcgg gacagtacta cgacacattg 360 aggccaaaaa gttagaaaag g 381 398 239 DNA Murine 398 gaattccccg actcgagcgg ccgctttttt tttttttttt tttttttttt tccaagcaaa 60 ccaacacact ttactgtggc gcaggctgcc tcagactgtt acttatttca gcccaagaac 120 tagaaggact tgaccagctt ggacaggcat ctgctcmgct ccaggcttcc acgagtcctg 180 gcacagaagg gttctctgaa aagtctacca caggaactgt gtctcggcac atgccaagt 239 399 391 DNA Murine 399 gaattcaatg aaacatacat tcagaagctt ttctcattct cttgaacaac acaaagtgaa 60 aagtgataat aatggtgcag aaggtgtaac agctttttcc tgtaatacac aggtaactct 120 cctcctaaca gtatttggtg aagatgatca atctcaggat gttataagat tgcgtcaaga 180 tgttaatgat tataaccgga gattctcagg gcagcctaga tctgtaagta atattatagc 240 agctacaaag tcagagagag cctttatact ttttgtacaa tcagatttat caaccagcta 300 ttgaactatg taaagtctta gtatgtvtcg actaagtttt aaccttcatc attgccagth 360 gctagthhcc cagagagcag agtttatcta t 391 400 264 DNA Murine 400 gaattccccg gctcgagcgg ccgctttttt tttaagtaga tttagcttgc ggaccccctg 60 gtgtgacaga gaaggcccag caaagtaaaa agtagctaaa agctgaggcc tatgacccca 120 aagcccttgc taacttcccc ttgctaactt cctcctgacc agaggtctcc tgcbgccagc 180 aggaatgaag cacactagcc ttagaggcag gtctgcgctg tgggtctgtg gaagcctcca 240 gcctttctca gcctcctgct aagg 264 401 266 DNA Murine 401 gaattcctcg gtcaaactcc ccacctggca ctgtccccgg agcgggtccg ccccccgcac 60 gcgcgggacg gacgcttggb gccagaagcg agagcccctc ggggctcgcc cccccgcctc 120 accgggtcag tgaaaaaacg atgagagtag tggtatttca ccggcggccc gcgaggcbgg 180 cgtgccccga ccccgacgcg aggacggggc cccggcctcc cacttattct accctctcat 240 gtctcttcac cgtgccagac tagagt 266 402 341 DNA Murine 402 gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca 60 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag 120 tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc 180 caaccgttcg ccagtttgcg tgtcgtcaga ccgtctaccc gacctcgttc aacaggtcca 240 gggcgcacgg atcactgtat thggctgcaa ctttgtcatg cttgacactt tatcactgat 300 aaacataata tgtccaccaa cttatcagtg ataaagaatc c 341 403 369 DNA Murine 403 gaattcattt tatttgaagc aaccttaatc ccaacactta ttattattac ccgatgaggg 60 aaccaaactg aacgcctaaa cgcagggatt tatttcctat tttataccct aatcggttct 120 atthcactgc taattgccct catcttaatc caaaaccatg taggaaccct aaacctcata 180 attttatcat tcacaacaca caccttagac gcttcatgat ctaacaactt actatggttg 240 gcatgcataa tagcatttct tattaaaata ccattatatg gagttcacct atgactacca 300 aaagcccatg ttgaagctcc aattgctggg tcaataattc tagcagctat tcttctaaaa 360 ttaggtagt 369 404 210 DNA Murine 404 gaattccaca gatgtacaag cttaaagatt tgaaagggaa acctgagagt gaacagagga 60 aagaaagaaa gaaggaaagg aagaaaggaa gaaaggaaga aaggaagaaa ggaagaaaga 120 aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga 180 gmgagcgagc atcattttcc aagttggttt 210 405 396 DNA Murine 405 gaattcgctt gctgtgactg gtccacaatt cctttcttgt catcaccagc agcaacctcg 60 gccaagtaac ggtagtagtc acccttcatt ttcaaataga agactttgct ttctggttgc 120 gaasattggg gatcaagaac ttttccaaaa gagacagtac atcgttgcag atgtcacgca 180 gctccgtctc gatcttctct ctgtattctc gagccatctc tgctttttct cagcacctth 240 cgtcttctgc tcaatacttg agacgaccct ccacgatgac ctacgggctc ctacaacgtt 300 tttataagca acagagagaa ggtttctctc ctcatwcgac agctcagctc cctctcagtg 360 acagacttya tkcaggctgc catgtcatca tatcgc 396 406 286 DNA Murine 406 gaattcgccg cttttttttt ttttttttcc cacggaactg atatatcacg atggagagaa 60 caatgtctat ggctgcacaa atccagaaat actagaagaa aactagccga aacttcttgc 120 taaatgtgta atgtaactat tgattactga catccttccg tttaaatcct atgtgttgaa 180 aatgcaatct tgggcagcct ggggacaaat gttcagtgga tgcttcaagt tgaaatctgc 240 tgcattggca tgaggtttgg tgaamctgcm aagtcacagc ctgtgc 286 407 200 DNA Murine 407 gaattcaaga cgtaggcagt acacagcagc agttcctgag tgtccctgtt tgtcacaacc 60 tggaggatgg tgaagttctc caggacactg ttcatcatgt agcgttcagg cagctgacgg 120 agcttgtgca ggaaattaac caggtactca cacatgggag agcgcasaga cggtacacaa 180 agcgcccgtc ctccagctgg 200 408 287 DNA Murine 408 gaattctttc tttctttctt cttcttcttt ttcttcttct ccttcttcac attttacagt 60 atgcatatct gtcttaagta caaatagaat taagtacaaa cagtatagga ataaaattgg 120 aattaaaagt ttgadctctt acatggctca gttggtagtg ctgtctgtgc aagcatatac 180 taagccagat atggtggtgt gtgattgtca tctcagcatt aagggkggca gagacaggtg 240 tdcccttggg ttwcsgctag ttagtcgagc cagaattgca agctcca 287 409 392 DNA Murine 409 gaattcccaa atgaactctc acttcttagg gcttgagttc cagaagtact ggggaaagac 60 taaagccaca gaagtgttga tgggggactg gggagattcc tcaatgggag aattcaggtc 120 cccaggtccc ggacttggca atgtgccttt taactgagat ccttggggct ggtgagacag 180 aatgtcaggc tcccgctgac ccagtggttc tcaatcttcc tgtgctgtga cccttaaata 240 tagttcacat tgtagtgacc ccagccatga aatttthgtt gctattttat aaccgtgagt 300 ttgttgttat gaactgtaat gtaaatttgt ttttcaatgg gtcacagggc gaccccccaa 360 agtggtggcg gcacaggttg agaaccactg gg 392 410 382 DNA Murine 410 gaattcgcgg ccgctttttt tttttttttt ttttttattg tcaagtattt atttatacct 60 acaaaagaaa acaagatggt atcaaaagga caatttacaa actaagaata gtagtaacat 120 agctctgagc atcctgtgca taacatcaca cctacaattc aagtctcaat gacaggaatg 180 tgtggagaga ccagcaaggg cgttagcaga gcactgatcc caagcaaaag ccaccaacct 240 tttttagatg agaagtctgc acaatggatg gttagggaga agcagcccac agcctaacac 300 ctagbcttcc taagtgagta accataacgg cattaaccca gctggaaggg tttgctgcac 360 ctgtgctgac aaaggacaga ca 382 411 264 DNA Murine 411 gaattccccg gccctggcac agaggactag gtgtgagagt gtgaggttcc cacccccacc 60 tttcctgcgc bgctccctcc ccccgacaca gccaccctcc gtgctcaccb bctgggagct 120 tgttgcttct tgttcaaggb gcgtaattbc gacactctct agggcgcagg gagccctgat 180 ttacatattt ctccbgagtb cbttccctgg tagggattct ctcttbggtt ctgacaccag 240 ggacaagagt bcaractgga aaaa 264 412 337 DNA Murine 412 gaattcagaa ccagaagcca aaarccaata aaaacaaaaa tactamcaag tcacttwcca 60 gctttaaatg tttaaatatt gcatggatca attttagaag ggcattgtat gtaaggcata 120 ctgtrgcatt tcagtcacca aaagaaacaa tcttcctaaa tcactagctt ctaggctgcd 180 cttctcaatc atgtgtctgt ctgtctgtct gtctgtctgt ctgtctgtcg tagcccagac 240 tgactgacct ttgtttccac cttccaagta ctggtatgat aagtgtrcwg rattatcctg 300 gcttagtctt tgaaagtaga achgagcaat agggaac 337 413 280 DNA Murine 413 gaattcagct cacggaagat gttgctaaat tggaaagaga aatggagcaa aaacacaggg 60 aagawctgga gcaattgaag caattgactt tcaaggacag taagatagat tctgttgctg 120 ttaacatttc aaacttggta cttgagaatc akccacctcg gatttcaaaa gcacaaaaga 180 gacgggaaaa gawgkctgca ttggaaaagg agcgggaaga aaggatagca gwgkctgaaa 240 ttgagaactt atctggagcc agacaccttg agagtgaaaa 280 414 408 DNA Murine 414 gaattcgttt tattgggaaa tgtatgcaat tcactttcag tttttgagaa cacctagcaa 60 gcatccaaga agacagcaca cacagtttca aaggaacaag gacagacaaa agggctggtg 120 gccatcccag ggacattgcc ttgaaaagta agtaaactgg gtgtcataaa taagactttc 180 ttactttata agaaggaaga atcaagatcc tgttttgatg tgtattaaat ataaaatata 240 aaatactctc tgacccagac gagggtggrv gaaatcctcc atccaacacc tcaagtttca 300 tgcaataaaa tccagaggtc tgttgaatcc gcctytcgat ycatgtactg cctgtactyc 360 ctcttttgag acacgttgat ggcataggca ttacagagcc gtctacct 408 415 247 DNA Murine 415 gcgtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag 60 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt 120 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc 180 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctaccc gacctcgttc aacaggtcca 240 gggcggc 247 416 374 DNA Murine 416 gaattcttca tgtgtaagca atacctactg gtgatgtcgg atgccctgga gctggagtta 60 tcggcatttg tgatgatcct atttgtaggc acagggaaca aacttctgca agagaagaaa 120 agactcttaa ctgctgagcc atctctcagg cccccaacct ctccattttc tgctaattaa 180 acctttccct hmctcagcct tgattcatgc ccataattta cctcgacaca tttcattctc 240 aaagaaatac cattactcct tagggattgt ctcttggatc cttctgagat tgatcgttat 300 gaatgtaaaa gcacgggggg gggggggcag aaatcacaac tgtaaattca catcctacct 360 ctcgtgcctg gaat 374 417 381 DNA Murine 417 gaattcctcc tacaacttca ttaactgcgt actccttatt atcaacattt ccctgcgact 60 tcttacaatt ggcatactcc tcaagaatgg catcgacatt ctttttagca gggagctgga 120 acaactgctt ctgcctcgta accaagtccc agtcctccac cagccacggt tttaattctt 180 cagggatctt caccttcacc tccatcctac tcttgaatgc ctccgctctc cacagtgggg 240 tcagcccgtg cccttttctt ccgagggggc tgggggactt cactggtach gcctccgtct 300 ccgttgccag gagccttcct tgttcthchg gtctthvgca cagaaccgga aggarggttc 360 tcagcagagc gagcctcccc a 381 418 190 DNA Murine 418 gaattcgctt gctggagaga gagcactccg ccgggggtcg gtgaagtatc ccaagatggc 60 tgggcgtaaa cttgctctaa aaaccattga tgggtatctt ttgtggaggt catgccccaw 120 aaccagaagg caatggaaat vccctgaagt cctggaatga gaccttccac ccaggttggc 180 tagtctgtct 190 419 191 DNA Murine 419 gaattcgcag cttgaggcac agacgaactt caccaagaga gaactgcaag tcttgtacmg 60 gggattcaaa aacgagtgcc ctagcggtgt ggtcaatgaa gawacattca agcagatcta 120 cgctcagttt ttmmctcacg gagatgccag cacatatgca cattamctct tcaatcttcg 180 acacacccag a 191 420 252 DNA Murine 420 gaattccggc tcgagcggsc gctttttttt tttttttttg gctgtgtaca cagggtgctt 60 tattctccac agagtgatac atgctaaggt gggctgggct tggycgatgt bcccatatgt 120 acagaactga ataaagtggg tctctgagag gtctgagtcg ccttggtgtg aaarggacat 180 gggaaggagg aggctgttaa gaccagagtt gttagtctgt gctgtctgac tggatgtagg 240 gaggtaggca gc 252 421 379 DNA Murine 421 gaattccccg gctcgagcgg ccgctttttt tttttttttt ttatctttca agcttttatt 60 taagtgcact gacttaagaa tgatttaaat cttgttaaaa gcagccacat ccatggactg 120 tacgtagtcc tcaaaagcag taatttgctc ttccagcata tccgttccaa ccttatcatc 180 ttcaactaca cactgtattt gaagcttttt aattccatat cccactggaa ccaatttaga 240 ggagccccac accagggcat ctgcttgaat gcttcggaca cactcctcta gttttgtcat 300 gtccgtctca tcatcccaag gcttcacgtc tagtaggatt ggaagacttc gcaacaactg 360 caggcttttt agctttctt 379 422 296 DNA Murine 422 gaattcctga gagcaggtcc tgtagagcct ggcggacagc attacactct gccacaatgc 60 ctcccgacgg tcatcacgtg tgcaggatga gtcagccatc agggcagccc cactaataat 120 gctttccagg cgctcctcca gggacggcct aaagcgctcc tyytgaagct caagkkgtcc 180 acaatgattt gtttatcaaa gttgttgaga gcgtatccag ctctccgcca ctgccaccct 240 ggtgctgggc agcatcatct gatgcagtmg cctgggctgc attagaaatt tcctgt 296 423 296 DNA Murine 423 gaattcttca gaactaaaaa aaatatttca tttcattctg aataaaaaac agaacagaca 60 gaactcttgt aaattctgaa aacaatgtcg tcgctacgga aaatttcaca gaaatcatca 120 gggggtgtgg ggaccaaggt gcctgccctg ccacgagcgc cacctatctg cagtcccaga 180 ggaggctttt agggaccagc acaggtggtg gcagagcctg aatcaagctc aggacgcagc 240 ttctacctgc tgcaccaaga cccggtggcc cagagggcag cctagggtct ycagga 296 424 299 DNA Murine 424 gaattcccat cagaaaaaaa aaaaaacttt gcagccagct ctacttgaaa gcatggagat 60 gtgaataaag atgcctaggc ttgctagtgt gattagccat ctcctgacct ggaaataaga 120 cccaaaaggc aaaacaagaa taaaacctga cagacacctc ctatttacat ccagctatgt 180 acaattcaat aaattaaagt ttaactttct gagcagtcat attccaccta tttacaagag 240 atatcaaata attacataaa tcctttgtcc aatgtcgtgt btcckcttta ttattatct 299 425 256 DNA Murine 425 gaattccgcg gcctgggcct agtggcttaa cagtagcgac agcagcagcg gcggcggcgg 60 cggcagcsac ttcccgtggc gagcacaggc ccggaagccc gcacaggcga gtagagaaaa 120 tggcagacga tattgatatt gaagccatgc ttgaggcccc ttacaagaag gtgagaaaac 180 acgctagtga ggctttaata tatttcttaa tttagcatta ttcacgaaac twctgctgaa 240 atgtaaacta accttc 256 426 238 DNA Murine 426 gcgtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag 60 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt 120 gcgtcgagcd gcbcccgctt gttcctgaag tgccagtaaa gcbccggctg ctgaaccccc 180 aaccgttcbc cagtttgctg tgtcagaccg tctcccgacc tcgttcaaca ggtccagg 238 427 348 DNA Murine 427 gaattctttg ctacaagctg ggacagctgc aagaggagtg gcagagcagg ctcccgttgt 60 ctctcaagtc tttttcccct gactaattgg aattcatagg ggtaatttat agagggtgtg 120 ggaagtacat tttgttgcaa cctgacagtg actgtgagtt cctcattaac caccatacat 180 gggctctgtt ctaagtctgc tgttgtatca actgtctaat tgtctaattt gtctaattta 240 gtctttagtg ttcttgaagg atttaggtac cagtgtacca tttagcaaat aagcaaactg 300 aggcacsaaa ggttaagact gcttaggaaa ccataggcaa tgagtggt 348 428 241 DNA Murine 428 gaattcgctt tttcttgtgt gaacagtagt ggtgaggcct atgtttttat gtggctttag 60 agaaaacttc agtcttcaaw gaactcttct aattagttcc ttcttagaaa aagttatgcg 120 ttaatttgtt tcaaaatatt taggcattct ttgaattata aacttgtgat gcagggattt 180 atgaatgaga cgttcacatg tgaagatgac ttcactawgc atctgtgtaa gcagaataag 240 a 241 429 329 DNA Murine 429 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg 60 tcaagcatga caaagttgca gccgaataca gtdatccgtg ccgccctgga cctgttgaac 120 saggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag 180 ccggcgcttt actggcactt caggaaccag cgggcgctgc tcgacbcact ggccgaagcc 240 atbctggcgg agaatcatac ccattcggtg ccgagagccg acgacgactg gcgcccattc 300 tgatcgggaa ttcccccagc tthaggcag 329 430 261 DNA Murine 430 gaattccgcg gcctgggcct agtggcttaa cagtagcgac agcagcagcg gcggcggcdg 60 cggcagcsac ttcccgtggc gagcacaggc ccggaagccg cacaggcgag tagagaaaat 120 ggcagacgat attgatattg aagccatggc ttgagggccc cttacaagaa ggtgagaaaa 180 acacgctagk gagctttaat atatttctta atttagcatt attcacgaaa cthctgctga 240 aatgtaaact aaccttcccg g 261 431 317 DNA Murine 431 gaattcgtta gcggcggcgg cgggaatcca gcggctggct ggctggcgac taggcctctt 60 gcagagaatc cggcgggaat ctgagccatc cgagccgcca ccatgacggt gggcaagagc 120 agcaagatgc tgcagcacat tgactacagg atgaggtgca tcctgcvgga cdgccgtatc 180 ttcatcggga ccttcaaagc ctttgacaag cacatgaact tgatcctgtg tgactgtgat 240 gagttcagga agatcaagcc aaagaactcc aaacaagcag aaagggaaga gaagcgagtc 300 cttggtctgg tgtycct 317 432 358 DNA Murine 432 gaattcgggg gatatagctc agtggttaag agcactgact gttctctaga ggtcctgagt 60 tcaaattcca gcaactataa cagtggttca cagccatctg taataggatc caatgcccgc 120 ttttggtgtg tctgaagaca gtgacagtgg actcatatac ataaaataat tcttaaaaga 180 atgttaaaaa aaaagaacat ttattttaaa taaataaatc aaattaaaga attattttat 240 cattattaac tgtgtatatg tgcacgtgaa tggagatgcc tataaaggct cattggaacc 300 cgtggagcgg gagtcttaga caactgtgag ctgccatgta ggcactggga agtgaact 358 433 280 DNA Murine 433 gaattccttt gaaacaaaac gacttattta cggttacttt ccttataaga aggaacagca 60 gtctctaata atcaccataa agtgaagtgc tgtgtcccta attttctcca gtttcttcta 120 ccctaagaca tgttttttgg agaccacaat gacttttgta tttaataatg taagtttcta 180 ttcagataaa atgatccagt ttcaagacag gtgagaagcc ctatttaagt ccaatggctc 240 acaatatgga ctgagaacag gagacatttt ycctycaaag 280 434 252 DNA Murine 434 gaattcgcct tgtccccaca cacgacacac tgctcgtctt tgtccaggta actagggata 60 taccctgaca tgctgctttt caggggacat tggccgttct ttctttttcg ctttccatct 120 ggtgacctgg cactgttctc ctctgggtct gacccacact ccaccttgct tggcttctgt 180 tccattcact tcaattccat ccaggatgct ctccagcrcg ccaagagact ggggtgggca 240 cactggcccc cc 252 435 392 DNA Murine 435 gaattcctga gcsgcacttc atcgatgatg tacagatgcc cctgggtctg gtggtggctt 60 cctgcagcca gacagtcacc tgtatcccca actgcacttg gcgaaactat aaggcggaag 120 tgcgcttcga gccacgcccc aagcccgccg tttcctcagc accaccatcg tctaccccaa 180 gtaccccaaa accgtctaca ccaccactct ggattacaac tgccacaaga agctgaggag 240 gtttctgtcc agtgtggagc caggccacgg agttcctggg cgcgatgggc tagccgatga 300 atgttgactc agctagcttg aggttggacc agctgttcat acactgccct ggtccccaga 360 ccaccctgga caagctgggt agcattgctc tt 392 436 238 DNA Murine 436 gcgtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag 60 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt 120 gcgtcgagcd gcbcccgctt gttcctgaag tgccagtaaa gcbccggctg ctgaaccccc 180 aaccgttcbc cagtttgctg tgtcagaccg tctcccgacc tcgttcaaca ggtccagg 238 437 327 DNA Murine 437 gaattctttc aaagtatata aatagaaaaa ccctaaattg aactgaacag gttatttaat 60 gagcagcagt aatatatata tatatatata tacacataca cacacacaca cacacacata 120 cacacaaaca caccaaaata cgacagaaga aataacaaaa acaaaaacca ttataaaagc 180 agtaatatta gggaaaaagt ccaataagta aatgtataag caataagcac ccaagaaatt 240 aaaaacactg aaaaaacctc tcagaaaagt tctgtcgcgt ttgtgaacct tttttttttt 300 tttaatcaaa tcgacaacaa acattaa 327 438 380 DNA Murine 438 gaattcattt tatctaggtg gactctgaaa aatgctgtag attttctttt tttttattaa 60 taacaacaac aataatataa aaagtcaaac aaactgcaaa cacacgtttt ctcactcaga 120 aaacttttta taatttacca gaaagattgg tgactctttc caaagtgcta aaaaagttgc 180 ccaattacat taagcattac taagtcattc aaatacaggt tcagtggcaa gcaatgaaat 240 gcacggcatt tgagcagtaa gcgtctccgc ccacctcccc tctgcacggt cccaccagaa 300 gacctcttat tgcacaagtg acatgctgta aaacctaggg tcctcgtkgt cagggacacc 360 cattcaggtt cttaacctgc 380 439 150 DNA Murine 439 gaattcggaa aagtgtctta ccctagatgt ttagccatgg tcaaattaga cccctgactt 60 tctggaaaca aaatatgtag ttacctttta ctctgaccat catctcccac ctgcctaagg 120 tacttagtcc ttagttagac ggcctctatg 150 440 432 DNA Murine 440 gaattcaaag ggagaaaaac aaaagttcat gactgtgatg cccaacataa cagttctagg 60 gcaggtatgc cagggagccc ctcccatgcg ctgtctccca gctcccaccg ctgggcaagg 120 atcattttaa ggatgggcag ttctggggcc acagcaccta gttttgcggt taaagggagt 180 ggggggaggg gtgaacagga agactgagga gggctcgggg catggtgaca aaaagagcta 240 ggctgcccta cccccaactc gattgtctaa cagataaaat gcctggccat aaatatgaac 300 actgattgac tgttgaggca gattggatct aaaacttgca gggsagaaca aaatkgctgt 360 gacacccctg aatttggtat catagtatct ggggtccatg tcctaactta ggagtggatt 420 ctgtctaaaa at 432 441 323 DNA Murine 441 gaattctcga tctggaacca ccagccatgc ttccttaagg actgggaaat gcacgtccac 60 ttcaaagtcc atggcacagg gaagaagaac ctccacggag atggcattgc cttgtggtac 120 acccgagacc gcctcgtacc agggcctgtg tttggaagca aagacaactt ccatggtttg 180 gccatcttcc tgggacacgt atcccmatga tgaaaccact grgcgtgtgt ccccgtacat 240 ctcggtgatg gtgaacaawg gctctcctgt cgtacgatca tagcaaagat ggacgatgga 300 gtgagttggc aggctgcacg ctg 323 442 412 DNA Murine 442 gaattctttg caaccaacat gaaataaaaa aaaaaaaaat ctgtaagctt aaagtttaat 60 gtggtaagca cagcatggct gaagaacacc aactctccct ccatgggtgt cattgcctgt 120 tgacctgtgt gtgtcctccc tcacatgatg gcaggtcatg cgagaggccc ctggttccca 180 tgaataaggg ggggggggta ggtgaatagg ggacttgaca atgcagggct cttccctttc 240 catcgtcttt gtctgtaact tttaagacaa aatttgaaat ttgaaggtag tctcaaatcc 300 tggaaggttt aaaatttgat ataagataaa aaatggaaac ttttattaaa ataagtactt 360 taaactaaca ctgaatagtc tagaccgtta acagaaggaa aatcttgtgc aa 412 443 444 DNA Murine 443 gaattccccg gctcgagcgg ccgctttttt ttttttctac ttgctaagcc atatcgaatc 60 atatgttttt ccccccaagc aatcagtttg ctttctcaga ttttatttga aaataaaggt 120 ccaggtcatt tctaggactt ggaggatttc ctgtaaatct actaaattag cacatcaatt 180 aaattgccct aactcgcagt gtggaagaca acagtgtcca ttgctacggg atcctggggg 240 ttcttgcaat ataagtgttc ctcaatgcgt ggctgtttcc caaatgtcca cctccaaaaa 300 agtcatctgt aatcttgtta aattagaaca cttccagtat ctttctgact tttacagtta 360 aggttacaga attgatttaw tttatagtcc atggctctca gagcttaaca ctagcaagac 420 cccatggcta gaatgccccc aggg 444 444 433 DNA Murine 444 gaattccata aagcaaacat tgaataaaga tgaaatagca ctggtaaact taaaaaataa 60 aaaaccaaaa acgttctgtg ctcttttatg tgtaagatgc taaaatcaag tatctttcca 120 gatggctcac caccttgtat ttatgcaggg tcttacactg aacctagagt ttacaatttg 180 gccagcttgc tttgtgggat actatctcta cattcccagt gcaaggatta cacttggsct 240 acatatccac ccatttttaa gggtctgaat ctggttttca ttgtctgcta gtgctttatc 300 tattggacta gctccccagc cacacagtaa ggcatacttt aaaaggctat cacacctgtg 360 atctaattct gatttcacag gctaagaagc tattaaatcc aaggaaccat gaactagttw 420 aacaaaaatg gct 433 445 420 DNA Murine 445 gaattcaaaa ttcatttcta tatcctcttc gatgtacacc atctccacag acttaattct 60 ttgaagccag agacctggta gactgtgacc cagtaaaaat ggcttttgcc tttatgtaca 120 tcagatccgg gcagggcagt gacatcaact aacacggtgg tttcttacaa gagcaacagg 180 gtgtgtgtgt gtagggtggg gactcctctt ccaaagatcc agccttcaga ctgacagctc 240 tgccctttca tctcacctcc tgagcaatca cacaggttta ccaatgttta accacatact 300 taacaagaaa gggcaatcct tctgtaaacg ttctctgctc aaggtaacaa acatgccctt 360 ggattggttt caggagatca gctagggacg acctgtgatc cccgtctcca ttcctcccag 420 446 317 DNA Murine 446 gaattctttg gggggaaatc cccaaatttg ggccccattc tagaactctg gggagttcaa 60 attccagaga gaatatatat tatatatgtc ccccaaattt cccatccctc caagccccac 120 gatctctaga agccccaaat ttctaattcc caggacttcc ctacccaagt aacagaatct 180 tcaaatcccc agggaatcca aacttaagac cccaatccca agctcaggaa acccaactac 240 maggtcctaa ggctgggagg aaggaccctg ttgccaggct ctcagggcat ctcaaacact 300 gactaccagg caccagg 317 447 290 DNA Murine 447 gaattccgag cggccgtttt tttttttttt tgttttgttt ttgtttgttt ggttggttgg 60 gggtttttgt ttgttttttc gagacagggt ttctctgtat agccctggct gtcctggaac 120 tcagaaatcc tcctgcctct gcctcccaag tactgggatt aaaggtatgt gctgccaccg 180 ctcagcattt wcgtatattc ttattcttca aaactaatct ctacagtcaa tttagcaagc 240 tcaaagatag caatgatcca aagaagtaca gactagaagc agatcaattt 290 448 396 DNA Murine 448 gaattcaatt aattagaggt aaaattacac atgcaaacct ccatagaccg gtgtaaaacc 60 ttaaacattt acttaaaatt taaggagagg gtatcaagca cattaaaata gcttaagaca 120 ccttgcctag ccacaccccc acgggactca gcagtgataa atattaagca ataaacgaaa 180 gtttgactaa gttatacctc ttagggttgg taaatttcgt gccagccacc gcggtcatac 240 gattaaccca aactaattat cttcggcgta aaacgtgtca actataaata aataaataga 300 attaaaatcc aacttatatg tgaaaattca ttgttaggac ctaavvcaat aacgaaagta 360 attctagtca tttataatac cgacactaag acccaa 396 449 373 DNA Murine 449 gaattcggaa agatggtcct tctcagggca tcctgggaaa cctggctgag aaagaaggtc 60 tggtctttaa agctgtcagc tgcttggaga agttttacgg ggtttctgac ttcaaatcga 120 tttctgaaca gcccgtcagg cttcttagtg tgcttttgct caaagacttc ctcatcctcc 180 agtgaggtcc tggcgtagtg gccagtggca acggcatctg ctccaagatt gtccacagca 240 tagtgataaa agcaactgaa cttgatatgc ttattgcagt tgatgtcggg gtttgagtcc 300 ttctttctca taccgktcaa aaagtcactg aacacatcat tccaatactc cttcacatag 360 gacacctggt gga 373 450 420 DNA Murine 450 gaattccagc acctgcgtas cgcacgtggt acgtccaggc cacctgtgcc acccaaggca 60 caggcctgta tgatgggctg gactggctgt cccacgagct gtcaaagcgc tagccagcca 120 ggggcaggcc cctgctgccc ggaagctccc gcgtgcatcc cgggatgacc agactcccgg 180 actcctcagg cagtgccctt cctccccact cttcctcccc acagacaggc ctctgctcct 240 gcgcctgcct gcatgctctc tcttgtcgtt ggagcctgga gccttgctct ctgggcacag 300 agggctctgc tctcctgcct gctgggacct gtggatgggc ttcctggcca aggccccctc 360 ttccagggga ggagcaggga tctggattta atttggtttt ggttttggtt ttttgatttt 420 451 405 DNA Murine 451 gaattcctca gtttcttcaa atatacatgc tttcaagcac ctcccaggtg tagtggcccg 60 gagtgagttt acttcagatt attcattaca actagctgtt atttgtttat aatgcccttg 120 tgattgtaca ctttgcatat gttactcctc ttattactca gagtataaac tgtctgatgt 180 tctgaataaa gttagctatt gcatgagact tcagtctgtc tcatttaatg gctccattct 240 cccaggtccc atcacagtaa acaatacata atggattttt ttgtttgttt gtttgttttt 300 ttgttttttc gagacagggt ttctctgtag cccggctgtc ctggaactca ctctgtagac 360 caggctgttc tccaactcag aaatccgcct gcctctgcct cccaa 405 452 446 DNA Murine 452 gaattcgctg tggcacccat tcatgtaact tcctcatttc atgtaaacaa agttgctggt 60 gactgtggct cctgacctgt acgtcttatt tggatttttc tctgatagcc catctaagaa 120 cttgaattca caccctttgt gcagggctgt ggttgactcc tggtgagggg tggagtgatt 180 tctgtgactt gagaacgaat ggacacaagt gctaagcagt ctgctgggct ctgctgtcgt 240 ttagtgttct gttttccctg acatggtgtc caatcctgaa tttattcact ggctttggtt 300 ccattgaagt ctgagtcccg agcgtccatt tcttcttcag aaccatctgt gttttcaata 360 actctacggc ccccagccct tctggaagga acaaatgaag cctcgtttcc hctcctggtg 420 gctcactgcg aagtttcctg tggggg 446 453 464 DNA Murine 453 gaattcgttt ctcctgggcc tcgatctgcc ggatgacatc ttccatccag agcatgaggt 60 cacgcaccat gctgaagaag cggaacttgt ctcctgtgtc taccagccgc accctgcgac 120 cctcacaagc atccagcagg gacttccagg cttccaggac ctcattctca cgcttctgga 180 tgtcatcagc cttgtcccct gcataggctg cctggaggcg agctgcatcc tcctgcagct 240 gcctcacctg agtgcccaga gcttggatgt cgtgctcaaa ggtggtgtgc attctctgta 300 aagtttccac agtgttttga tctcttccaa gctcctcagg gagtttcttg tgtttgtcct 360 ggattcggcc aaagatctcc ttggcatcat ggtaaaactt atgaagttca tatgagcasc 420 aagaatctgt gttcttgtgt caatgagctc caggaggtca ccca 464 454 369 DNA Murine 454 gaattcgtgt gtgtgtgtgt gtgtgtctgg agtttacctg ctacatcaga acgacccccg 60 atcccagcca ttgcttgtgg cctctcttta tagtcagata ttgcctttgt gtgaaccctg 120 gaactattga aacacttgtc tcttgttctg ttctgttcag ttgtaatcac tgttacatgt 180 ggagccacac agtcacctcc acgggctgta ggagcwgctt tgtggtctgt gtccatacat 240 gggaccctta cttggagtag gctctaggtg catttggcta agaacaagcg agtaacacta 300 gaaacaaagc tctgctgggg tgagctggag awcatggatg ctctgccagg gtgagcagga 360 gawcatgga 369 455 295 DNA Murine 455 gaattcggaa ccttaggcat tgcagtacag accccaaggc taacccacaa cttaaagtgg 60 aaaatcttat rgtttttccc ccttggtcag acacagatat atttgaagaa tttccaaatt 120 tagagttctc aattttgggt acatcaagac ttttaaagta gaatttacgt agtaacagaa 180 gagaaaaatc tgggaccttg aaaacagtac atttcacctc ctttgggsta aaagtcacct 240 tcagtttaag ggsggcattc acagaaaacc tcagctggag catctcgtgg cgcag 295 456 391 DNA Murine 456 gaattccttt cttccttcct tccttcctcc tggccttcct cttcttcctc cttttcccct 60 tcctcctcct cttccttagc ctcaggagac ttcacgggag acttttcggc ttctggttcc 120 tcctcctttt ctcggcctct tccttctcct ctttggcgga ggctgccaac tcctctgcga 180 tggctgtgag ggtttcttcc atttctgact tctcatcttc cmctttagtt tcttcgatga 240 tctcctccac aaatttgtgt tggaccttga gcttgggggc ctcgactttg gtcttctgaa 300 tcttactgga tattgtgact gagggctgtc ggtgtgtgta cagargcccg gtgatgcttc 360 ctgaaaatgt gctaaatctg gtctcttccc c 391 457 308 DNA Murine 457 gaattcagtg aatggtggaa atgctctcca gtggggtgtg gagagagcag gaagccagtg 60 ggcaggctgg agcaggtggc tcatggaagg gtgggttagg gaccttcagc ctgacttctc 120 ctggcggggt ggacgtaggg tgggcagaac caggaagccc atgacttcgt ccatgctgcc 180 tcccttctcc cctccttacc cagggtcctg catccttcag scccctatgt ggctgccctg 240 cacccttgcc tgtcccaccc ggatgccatg cacctgtccc cgtcactkgt tccctgcttg 300 gactgcag 308 458 206 DNA Murine 458 gaattctcag catcatctcg tagtagttgg tgaggttctg ctccacaaag tgaaaggtac 60 ggatactgag ggtctcagaa acaaggccgg ggaggaaggt ggcagctcgg ttgaaggcca 120 tgaagaaagc catttgccca catgtagtaa gtctcgtcat gctgctgcct ctctcccgaa 180 gcagatgatc cttgaccgcc ccatga 206 459 383 DNA Murine 459 gaattcgatg cttctataac ccaaggaatg ccacggattg ccagcaagtt cagaagttaa 60 gggagatgct tttttaggat cctttccagg gcccctggaa gaaatcaact ctgctgaccc 120 cttgacataa gacttcagag cagtgaatag tctctgctct tttagacatc tggtctgggg 180 tcctatatta gggtagctcc agcaaacttg taacttccct gagcaagtgg ttggcacaga 240 cctgttattt acttaatgca tagttccctt tgtccctata ttacatttac tacagtctca 300 catactacac tttacccatt attcatgagg gtaaacttga tgatcactgt ttattcagca 360 cctagacaga gttggggatc tgc 383 460 324 DNA Murine 460 gaattcgtcg gcttagcagg tcagaaagac gtaagcacag accatggcct atggaagaag 60 ctggactatt aggaacctgt tgtagaaacc caggagaaca tagaagacaa ataagggaaa 120 gtttgggggg atgaaagaat agggggggtg gcaaagatag ctccatgttc cttgctctga 180 gaacctgagg atagaagttg ccattcattg tcgttgaaag atggaaagga twaaataagg 240 gaaatgtcca gatctgtttg ggagcctgtt gaacatgagg aaaccaaggt ggggtgttca 300 gccctggatg atcgtaggag tctc 324 461 296 DNA Murine 461 gaattcctcg cgtcgcggct gcggagacta gaaggaggac tccggatccg gctcggcgct 60 cgccctcgct cgccatggag aagaccgagc tgatccagaa ggccaagctg gccgagcagg 120 ccgagcgcta cgacgacatg gccacctgca tgaaagccgt gacggagcaa ggcgccgagc 180 tgtccaacga ggagccaacc tgctgtcggt ggsctacaaa acgtkgtagg ggggccgcag 240 tccbcctkga gggtcatctc gagcattgag cagaagaccg acacctcttg atwaga 296 462 210 DNA Murine 462 gaattcagag aatacaatcc aattcactgc tacaattcat agaattcgtc agtgttttct 60 tgagacgctg aggttcactg ttggcagttt ccagtggccg catgtgctgc tcagaaaggc 120 cagcggcaga cagctgcccg gaagaacttt cactgctgga aaactgbtcg ctcccaagga 180 aagcccaagg aaggctgggg ccgtggstca 210 463 303 DNA Murine 463 gaattcatca attttgctaa tgatgtcaaa taaagattgg ttgtcaatgg gcagcacaca 60 gtctgcatgc tcattcagtt ccttcatggc cagcatactg ttataaggcg aggtgatggc 120 atcgtcttca ctggaaggat aaaccgctgt cacaaaccgg tacacttctg ggaattcatc 180 ttcaagaacc tttaacagaa atgtgccaag cccagagcct gttcctccgc ccatgggagt 240 ggatgatgaa gaagcactgt aagcaatcgc actgctctgc cgacttccgc agtttctcta 300 aaa 303 464 511 DNA Murine 464 gaattccttt ctttctttct tctttctttt ttcctttgga agattttact gcttttatgg 60 tacccccctc actctgtggt gtcgagctgt ccatcagcat cacgtgggtg agtctgggat 120 ctactgactt gacctcacca gtctcagtta tagacacttc cataagacgg gtgactgagt 180 cctgacggct cacaacacca cagagccata cttcctctcc ttcgggttgg tagaccttga 240 ctctgtggcc ctggacacta tagggacctc ggctgaaaat ctcttgtagc ttttggtcac 300 tgatcaaagc attaactgtc tctcttaatg cagcatgttc taaaagaatc tgattttgaa 360 catctgttcc catctggaac agatgcvtcc cattagcatc cgacaggaaa cgaagctctc 420 gatcacaagg tattcaactg gcaccacaga ccccaacscc agcttatcta ctaggggggg 480 tgaaagtcag gghggccact ggghaactgg g 511 465 269 DNA Murine 465 gaattccccc aatgtactct ctatctatta tatgtgtgca tgatttaaaa atggaggggg 60 agggaggcac aatacaaggg ctaagaaatg gctcagtggc aaacacattc tgcatgcaag 120 catgaagacc tgaatttgaa ttttcagaac ctatgtaaaa gctggaggaa tcgtgtgagt 180 atatgtaatc ccagcacccc tatggggtaa atgggaaatg ggacaggaag attctgggag 240 ctagagagtc atctagctgr gcataccac 269 466 226 DNA Murine 466 gaattccctg gagaagcctg gagctccaca tgcagagaaa tgatctgtcc ttgtgtctcg 60 ttctgattaa aaacaaaaac aatcaaataa aaaacaaaat kgaacaacaa ccttagtgta 120 tggcatgaga atgtgaaaac actagagatg atcaggggga tcttcaaatg gaggcagaca 180 gccagtttct gaagagaatt gcagtagctc ggaaagccag tcaccg 226 467 220 DNA Murine 467 gaattccgca aattccttaa ggaagtggaa gcaatcattg tttactttgc tgctggtctg 60 tgttttacca attgcagtta gtaaacaact agtctaggca tttatgtgct acatgaatat 120 aaccaaacgt gagaaaatag aaactgcaat ttttgagaac tatttttttt taaattccat 180 aggcaggctt ttaaaataaa aacaagtggg tcactttgac 220 468 344 DNA Murine 468 gaattcgaca tagggaacag gccatccaga caaggagtga gggtggaaat ttttgtattt 60 agagtcacat gtaaatttta aagctcaaaa aaataaacta gtaactccat gaaaaaaatg 120 agtgctttgg gggtggggta ggggataaga aagaaaatca gtgaggggcg aatgcccaat 180 tatcacttag catctcttaa ataatttcca ctggaggcag ggtatctttt ccaaagagat 240 gagccccatt ggatggattt gttacagttt taagtgatta aaatcgggac tttacagtac 300 atttgtgggk cttttactag tttttagagt ggtgtttkgc aaat 344 469 66 DNA Murine 469 gaattccaaa ttccctttga gccaggtatg agctcatttt yctacaagca tccaawwgtc 60 ttcttc 66 470 50 DNA Murine 470 ggrattcgtg aggccgaacg ctaaactaag gtacaaacgg cttaggccta 50 471 101 DNA Murine 471 gaattccaga ggggaagccc gaaaacctgc tgtgcttcct ggagttggca tggcggctcg 60 cccasggggc tcctcgcaca gactgactgg ggagggtgag t 101 472 213 DNA Murine 472 gaattcctgg ggctctgagg atcccttttc ttcctcttcc actttgacct ctgttaagga 60 tycacctgca tcccsgaaah tgccacattc tgccactcaa aatttgcatc atttcgggag 120 gsaawttttt catctatgtc ttcagtgaga gagtcatcta gatcagacgt gggsagagga 180 acccagaacc aacgagckty atgttggcct cat 213 473 188 DNA Murine 473 gaattcgaaa gagggaagaa tgaagcctga gctgaaccct aaataatatg tcagaaaatg 60 acaacttgcc tccctctaga ctatttcatt tgaaagattt gctaggttac attagggctt 120 gggatagatt tttctgggaa tggggsccta acccmcmgac ttaaaaaatg sccccgsttc 180 mcagttct 188 474 184 DNA Murine 474 gaattctttt tttttttttt aaaaaaatag tatgtatagt gtgtgtacat gtgtataagc 60 tcaagtaaga aagccagagg agactggsct tgtctgttct gctctccacc attaagccct 120 tgagacaggg tctctcacta tacctgatgc gatagccagc aaactccagt aaccctacac 180 ccag 184 475 319 DNA Murine 475 gaattcgagt agattcccag tgctcaccat gagggaaaca atgttactat acctttccta 60 tgaggaaagc cgggtaaacg tagaggtcct ctgtcatgtc tttaaacata gtttgagtag 120 acagcaatgc tctttaccta gcttagtgtt ctgatggcaa aatattgtat attgtgataa 180 ttatgtccta tttatttgag attcttgttt aaaatttaaa aaacaaaaaa acaaatdaaa 240 atttttttgc tatgccctag atgtagggct tttttttcca accaaaggtc tacaaaagtt 300 tctatagaaa ctgtgattg 319 476 401 DNA Murine 476 gaattccacg aggggcttcg gaaaggaatg ttttctggaa gtccttccac atagagatca 60 ttgggatggg cctcaaattt ttggtacggt acagccttgg cttccgtgct tcccaaggcc 120 tcggcaaatt tcttgcagaa gagctggtca accatcttcc tcagtttggt gatvcgagcg 180 taccactctt ctttcactcc tgaggctggt ttatcaagct gtaaatcttc tcgtgttgag 240 ttcagaagct catgtttctt aatcacgaag cggatccttt ccttcdccag caatatcctc 300 tcaaggcgag gaattccgta cgtcgacgcc ttctaaaagg aatcccttya ggaagyyctt 360 ctacgtaaag atcttcaaca tgggactgga aaagagggta c 401 477 385 DNA Murine 477 gaattcctgg gattaaaggc gtrcaccacc acgcccggct caggccagaa cctttacaca 60 tgcttaacta aaactagtga aaaatgcatc ttaaaaacaa gaaattccca aaatacaact 120 cagaaattac tccaccccat aaatgcagca aaaaatcatc tgatctattt taccagttac 180 taagcaaggt atagtggcag agacctgtaa ttcagggggg cagaggatgt cacaaattca 240 aagccagtct ggtctacata gcaagtctgc cccaactcaa tgcattacaa aatgaccccc 300 ctccccgacc tctcaaaaca aaacaaaaca cacaamacac aaagcccama caactcatta 360 gtaaaacaat ttgataattt atatt 385 478 391 DNA Murine 478 gaattccact ctaatttttt caaagtaaac gcttcgggcc ccgcgggaca ctcagctaag 60 agcatcgagg gggcgccgag aggcaagggg cggggacggc ggtgactcgc ctcgcggcgg 120 accgcccgcc cgctcccaag atccaactac gagcttttta actgcagcaa ctttaatata 180 cgctattgga gctggaatta ccgcggctgc tggcaccaga cttgccctcc aatggatcct 240 cgttaaagga tttaaagtgg actcattcca attacagggc ctcgaaagag tcctgtatwg 300 taahhhaagt cactacctcc ccgggtcggg agtgggtaat ttgagmgcct gcgccttcct 360 tggatgtggw aghcgtttct caggctccct c 391 479 443 DNA Murine 479 gaattccaca tctcaagaaa ctcaaagaat catactgtca aagacaggga gttccaatga 60 attcactcag gtttctcttt gaaggtcaga gaattgctga taatcatact ccgaaagaac 120 tgggaatgga ggaagaagat gtgattgaag tttatcagga acaaacgggg ggtcactcga 180 cggtttagat aattcttttt attttttatt tttccttccc ctcaatcctt ttttattttt 240 aaaaatagtt cttttgtaat gtggtgttca aaatgaaaat tgaatactgg cactccatct 300 cttagaacat atgaattcta gtgttcaata ttcattattg gttgtttttg ttgtgctgat 360 ttttvgtgat cagacctcag ccccttaata ctgccctttt gccctttaag agatttcatg 420 tgtgcacaga gaggccaccc ttt 443 480 382 DNA Murine 480 gaattcgatt cacagttgcc ccagagcaga gtgtgccctt ccacaaagcc ctagaggact 60 ggcagtatga catgatgcca ggatgaagct gtgatgtgga cgagaagata gaccggctgg 120 agtgagggag ggaacctcag cttggtcagg ccttgcaagt gagggcagac ggacagggtg 180 acctggctac tagactaggg tggcatttct tctgaatgat ccctgtgcct tcccagagaa 240 aggtgggaga aataaaggac agggtgggaa ggcaagggag gtgacagagc cagctccgtt 300 atctccccag gcctccacag caggggtatc tgtcagttcc atgcacccca gatctgggcc 360 caadcctgag ggtccccacc ct 382 481 521 DNA Murine 481 gaattcaaag cagctatggg cagcagcctc ctactagtta cccccctcag actggatcct 60 acagccaggc tccaagtcaa tatagccaac agagcagcag ctacgggcag cagagttcat 120 tccgacagga ccaccccagt agcatgggtg tttatgggca ggagtctgga ggattttccg 180 gaccaggaga gaaccggagc ttgagtggcc ctgataaccg gggcagggga agagggggat 240 ttgatcgtgg aggcatgagc agaggtgggc ggggaggagg accgtggact sgggtaagag 300 caaaaccttt ctccttttat ctaattttgt ttcatccata ggattttcaa tggaaagaag 360 ggactgaaag acataagaaa tttatcccac ttttcatgga caatctattc sdcaagctat 420 ctcctaaaac atggaaatgt catttaagtg cagtttgctt ttttccctgc cagtaaccat 480 tgttgggctg ggtgaacaaa gaatgctttg aaactagagc t 521 482 347 DNA Murine 482 gaattcgttt atattcttat cctcccagga tttggaatta tttcacatgt agttacttac 60 tactccggaa aaaaagaacc tttcggctat ataggaatag tatgagcaat aatgtctatt 120 ggctttctag gctttattgt atgagcccac cacatattca cagtaggatt agatgtagac 180 acacgatctt actttacatc agccactata attatcgcaa ttcctaccgg tgtcaaagta 240 tttagctgac ttgcaaccct acacggaggt aatattaaat gatctccagc tatactatga 300 gccttaggct ttattttctt atttacagtt ggtggctcta tggaggt 347 483 343 DNA Murine 483 gaattcatcg ggaatagtgg gtactgcact aagtatttta attcgagcag aattaggtca 60 accaggtgcc ttttaggaga tgaccaaatt tacaatgtta tcgtaactgc ccatgctttt 120 gttataattt tcttcatagt aataccaata ataattggag gctttggaaa ctgacttgtc 180 ccactaataa tcggagcccc agatatagca ttcccacgaa taaataatat aagtttttga 240 ctcctaccac catcatttct ccttctccta gcatcatcaa tagtagaagc aggagcagga 300 acghtgaaca gtctacccac ctcthgccgg aaatctagcc cat 343 484 386 DNA Murine 484 gaattcgttt tgggatagca tttgaaatgt aaatgaagaa aatacctaat taaaaaaaaa 60 ctttaaaaat taaaaaaaaa aaggaatgtg tgctggctgg gtgggtgagt gatgctgggt 120 ggttggtggt ggtccacacc tctaatccca gcttccggta gaggtgggca gatctctgag 180 ttccaggcca gactggtcta tagagccagc tgcagaacaa ccaggactac acagagaaac 240 actgtctcaa aaaacaacaa caaaatgtat gtctagcctc tthgccaact ctgtactctt 300 aactgtttga taaactgagt catagaagaa gcygtgaaat ctataatgcb acactatgaa 360 aggaccaggr aagcgccagt ctgcct 386 485 518 DNA Murine 485 gaattcctta tgaaatattc tgcatactta aatgaagctg gactacagtg ttctacgata 60 tcatcgaaga tgcacaatcc ccattgtctg tctggccatg gtctttgcgg acaaatcagg 120 ttgacaatta atgggagcag ctgttcaaac cacggcaaca ccttttcttt gtagctactg 180 aatattgagt gtaaaatatc cgacacttta gtcagtatat aaacatcatt atcatcctca 240 tcttgtagtg actcttcaac ctgctcgtca tagtcttcat cttgtctttt aacttgccgc 300 aactcctgat ttttgaaatg tkcttcaagc ttcgccttca ggatgcctcc cagctcctca 360 aagtgctcat tgttgaggca cccgtctccc atgacctcaa tgcactttgc aaaggaatgc 420 atgatctccg agaggacatc tgagtcgggc tctgtgccga tggccttgat gagagcmcgc 480 acatgaagtg ccacatctgt gtaaggtacc sggacccc 518 486 528 DNA Murine 486 gaattccccg gctcgagcag ccgctttttt ttttttwmwc ttttagtgga cctgagagtt 60 aaatcaaggg ccttgtgcat gctcacagta caccctactg ctgagctata tctccagacc 120 cagaatctat ttagtttata aataacttcc taatgcctgt ctaatgatgc atatcttaaa 180 taagtaaata tgttaaataa aacagtattc attttagttt taagtaatag gctatcttga 240 atttttagtt taaggtaaat caaataaaat taagactata aatgaatcct acttctatta 300 tttatcatac tgtatattga cttatgcttt tatattttaa cattggcatt caagtcatat 360 gaatcatgta aaattggctg cttttaacta ttgtagtttg ttatttgagt ggtattctat 420 gttgcttaga ttttaactgt gccatgtgtt ttatagttta tatggtttta tcctgattat 480 ctttttgtaa atgtgggagc taagaactta aagaattttg aaaatcga 528 487 396 DNA Murine 487 gaattactga tttgtgttgc tttaacaaca gcagactcat acatctcctt tttagtrggc 60 tgaaccctgt atctgaataa taagggatcg attgcatctt tcttcttccc atggtgaaaa 120 gactgctttg tgtttccgag tcgtcactgt ccctgatgac aatcgtctct ccatcagcac 180 tgctcaggtg thcgttagca aaaccattct gatgtaatgg agggaggact tccaagattc 240 tacactgcwg ccttgtgcca ttgtttccga atgacttcca cagtctcttc aacaaaatat 300 cggtccttga cataggcaaa gatatcatca cagatttcat gcaadcgtga acacgagtaa 360 ggttggtcag gtataaaacg gaataattag tggttc 396 488 388 DNA Murine 488 gaattcttta cagatgattg tgaacaacca tgtgcttgtt aggaatagaa ctcaggactt 60 ctgaaagagc agtcagtgcg accatctctc cagccatgtt ttacctgttt ataaagtggg 120 gctgtgtatt tagaagggtg aacacagtag agagagtatg tttctgcgtc ctgggcattt 180 gtgaactaga tgcccagcgg ctggtcctcc tccatcccct ccttcctgtt tcagtcaatt 240 ctagtgtaga tggcattttt aagtccatgt ttttatgttt tctggttaat ggttatcctt 300 cagatggtaa ttcttaccct tgtatttggg cagagcaaaa aggctttggc tctagactgg 360 ccagcagttt acctggataa rggtactt 388 489 420 DNA Murine 489 gaattcttgg ggttagtgag gtcaacttcc tcggagtcgt agtctgagag gatccacggg 60 aagacagggt actgcatgag gtcattgtaa gatctgcctg ccagcgtgtt caagtgcatc 120 aaatactgga agttgctgat ttcacctctc tcccatctct gagtcacaga cttctctcca 180 accagagtgc tgagtaaccc agacccttgt tccacactgg tgtttggtct ctgtccggac 240 acagactccg agctgtccgt gagagagggc acaactgcca ggaacctttg gtagacttta 300 ttccgaatdc ccttttgaaa agccaggagg tagttccgtc catctccaga gaaaacttca 360 acagcgatag gctggaggag atatctcctt ttatgcacct ccttgatgtc ttcatatgca 420 490 367 DNA Murine 490 gaattctttt tttttaaaaa tgacaataca aaagtacctt tacacaattt ataaaagcat 60 aattgatgat aaagcaagta ggagtctcac agtcaagtgg cacgggggct ggggccatga 120 gcagtccctg aacaccagct tggatgtcta agttcccagt gctgcctgcc cccgtmctct 180 agtttacagt gaaaaggccc atattccagg ccttggtgtt tcttttttta aacctttaaa 240 aacttgacat tacttctcat gaaaaaataa tgaaataacc ctcccaaacm actgacaaaa 300 atmattaaaa wwtgaccctt ttthamcaca acacaagcrg atcaaaamca aaggttccaa 360 aggattg 367 491 271 DNA Murine 491 gaattccccg gctcgagcgg cccctttttt tttttttttt taaatttttg gtttttcgag 60 acagggtttc tctttatagc cctggctgtc ctggaactca ctctgtagac caggctggcc 120 ttgaactcag aaatccacct gcctctgcct cctgagtgct gggattaaag gagtgcgcca 180 ccacgcccag cttatgggac ccccttttca ttgtagtctg gggtacaagt acagaagccc 240 ttgaggggct ctgaacctgt actgccccca g 271 492 378 DNA Murine 492 gaattcgcac agagcatctg tacatccctc agaactcaga gtgaacatgc tcagaatctg 60 gctctgacgg gtgatttgaa gaatctgtgt ttgaagcact tgactcatca actggttcaa 120 atggtcgcaa gtttgcatat gtcacctctt gggctagttg ctctagggaa gggcggccta 180 atagcaggtt tcggagtgaa attcgagtca tcaggaagct gcgtcgaaag acgtagagct 240 ttcgcagcac gaagcggaga aatcgctcat ggaaagggct atttcgcctg cgttcaattt 300 cttggagctt cctctgtcgt ctgagaaaag tttggaccag aagtgttggc tcagggcccc 360 ttttcttccc ttccaaag 378 493 459 DNA Murine 493 gaattccctt tactcatatt tatctcctta tttttaagag atttgttttc ttttaaaaat 60 ctgtgtgtgt ctgtgtgtgt ggagtgtgtc agaaaaggcc agaagagggt gtcaggtccc 120 ctggggctgg agttactggc tgaggtgagc tgcctcaaac agggctggga actgaactca 180 ggttgtctgc agaaacagaa agtgctctta actactgagc cacctctttr gccctctgcc 240 aatgtttagt ctaaccacta tttctaagct tctggttctc tgtgtacagc acaggaataa 300 aaacaacatc taaggctggr aaartggcac dcacctttaa tccagcactt gagaggcaga 360 ggcaggggga tcgaggccag cctggtctac agagtagtcc aggacagcca tgtagaaaaa 420 ctaataatga taacaacaac aacaaccacc accaaaccc 459 494 135 DNA Murine 494 gaatwcgtgt mgtggtctcc gaacdgcccg gaagcdccgc agtcaccgac gggaccagaa 60 gtggcatgac aaacagtaca agaaarvvca cttgggcaca gcctgaaggc caatcgtttg 120 ggggttctca tgcaa 135 495 326 DNA Murine 495 gaattacttt gatgataatc cacacaatat tgatgtgaat aaattaaagg tgttaatttc 60 caaagtataa ttacaaaaat aaaagtaaca gactggaaga gtattattta atggtctacc 120 aaagatctat aagcaagagt tttggggaag aaataacact attttgtatt tcactatatt 180 cattttaaac taaagcttgt aatctctatt tttaaaatca cattatatca ctttcttttt 240 tttttttttt gggttttwgt ttttttttwc gagacaaggg tttctctgta tagccctggc 300 tgtcctggaa tcactttgta gatcag 326 496 247 DNA Murine 496 gaattcctga ggagtccctg ggtcaatggc agcagaggag ctgcggcccc agatcacagt 60 atggcactca cacattttca agccagaact gaacagagga gttcgtaact cggtttattc 120 aggcgatatt ttggctatat tcagtgtgga tagcgatgct tcagagcaaa cacaaatcta 180 tgagaagtca gaggtagctt ttatcatctg tctaaaaggt ttaaagaaac caccttctgt 240 atgtgat 247 497 302 DNA Murine 497 gaattcgatg tgtgtcctac atgctggtgg ttttacccct acctgctgcc catgctcttt 60 cctgcttctc ggtaaggccg agcaacaagg gtttacagga aaccgagatt cttcccgagg 120 ctctcttggg ctcctagtga gggactcagt gagcgggagc ccttggaaaa gaagacggca 180 gagctgaagt gaaaagcagt ctcttcagga gggatgttcc ctcacccctt cacagcacca 240 aagtttcttt gcaaaatagg gtctgagcta caaaagggag gcagatgtgc ttgtgaatgc 300 at 302 498 310 DNA Murine 498 gaattcccca cagcagaagg gaggagacag ccaagaaaga gtgagctgaa agtcaggcca 60 ggataaagtt ctacccagaa gtgtctgaga gccatcaagc cttgtccacc atgatgggct 120 ccatccttca aaccatagcc agaacaggct ctttctctgg taagttgctt ctgtcaggaa 180 attcatctct gcaatgagta aagttcctcc tgcacctgca gaggatgggc aagcaccggg 240 gagtctaggg gtcatccagc ccacctgccc cgcaggbctg agctagactg agtgagaaag 300 ggagcacaaa 310 499 366 DNA Murine 499 gaattccccg gctcgagcgg ccgctttttt tttttttttt tttgtaaaaa gaaacatgat 60 tctttattga aggaacagcc gccatacaaa gatctattgc ttcctacacc gctacactca 120 gaaggaagcc gagaaagcta caatagggsg mgcatgcaga accacaaact ggaaagcaga 180 gagatcctct aaggcacgga ctggagcctg ttttcccagc ctctatgtcc agtgcctctc 240 tcagcccagg gagagcaggg gaaggcaagg ttgttctctc ctgcaccaga cacttagatt 300 tctctctaag aagaaaccac ttttccatcc actgattcct ccacactgat atggaaattg 360 ctgctg 366 500 384 DNA Murine 500 gaattccttt tctacaatgg tgctcacaga gacctgctta cactgtagct gcttaataaa 60 atccttcact tgcatgacca tgttctgagc aatatttatc tccagctcag tgtgcctcct 120 cttcatgttc tgcagttgtt ggtcagcatc ctgcaggtaa atccagagct cggccttcag 180 gctcttgatc tcctcccagc cctgagttaa gttctgtgct tggaccagcc tttgttcaat 240 cagctgctct gtttgctgaa tatcttttgc tgtgtttttc actgaggagt ttgacaagtc 300 acacatggag caaaggagat ccaagtaggt cctggcctgc tcttgcaaag ctctgaagtg 360 tttgacctgc ttaacagctt ctgc 384 501 400 DNA Murine 501 gaattccctc tttaaaggct ttgtcacaac aaacagagta aagtttacct cccagaacca 60 cctttcccac atgcagaggt aagaaaatac caaaagggcc caaacgaaat gtgggtggtg 120 gtgtgacata ggatagtggc agtcttcatg cctaaaacag ccctaggtag agccaggtag 180 agtggcaaac cctgtaaacc cagcactacg ggagcagaca ggtgtgagtt ccaggccagc 240 ctgggatcca gcaacactaa gtcttaaact atacatgcgc atkckckcck cacacacaca 300 cdckctgtga aaggggctga gtaaggtaca gacctttaat cccagcctgg ggaggcagag 360 acaggccagc ctggtttaca aagtgaattc cmggccagtc 400 502 432 DNA Murine 502 gaattcatta tccttcgcct aggacgtgtc actccctgat tggctgcagc ccatcggccg 60 agttgacgtc acggggaagg cagagcacat ggagtggaga acgaccctcg gcacatgcgc 120 agattatttg tttaccactt agaacacagc tgtcagcgcc atcttgtaac ggcgaatgtg 180 ggcgcggctc ccaacatctc cccctttcct tttaataaga gcaaataggc cacccatatt 240 aatgagagtg gagatagagg tcaaatcccc agtgtgtagg taaaggagcc atgtacagga 300 ttagctctta ggctcacagg cttttaccca gagcaaccct gacctgctcc cgtgtcgttt 360 ttcctggggg aagggaacta ggacactgaa ccttcatgaa agatgacatg tctccctaga 420 ataggctcat at 432 503 416 DNA Murine 503 gaattcaaaa aaaacaacaa cattggctta agttcatcct gatttcacat ttaaaaagaa 60 tactggagcc gggcgtggtg gcgcacvcct ttaatcccag gtctcgggag gtagaggcag 120 gtggatttct gagttggagg ttggcctgat ctacaaagtg agttccagga cagccagggc 180 tacacagaga aaccctgtct caaaaagaaa aaaaawaaaa aaaaaaaaaa agaatcatgg 240 gtcagtgagt ggaggtactt accctaaatc tggcatcctg aatttgattt ccaggactca 300 ctggtagagg gaaacmdctg actcctgcaa gttgtccttt gatctctata tgtgggttgt 360 ggcatgtgta tccctgatgg gcaataattc accaagtaaa ttaattaaaa tataat 416 504 434 DNA Murine 504 gaattccaga aagcacacag cacaataatc ttaagcacta ttgaggaaag gagagcccct 60 gatcaggcta cctttggtct cttaaaggct cctgagtact agtgggacat ggaaactctc 120 cattactgag ttgtttcagt gtcattctag cttcctgatg agatggcatc taatgggaaa 180 atgaactcgc ttggctccca caaggagagg ggaacactta gctgctgcct gtctctaaag 240 gcatgactgt gtagcacttc actaccccct gaactactag cattagaatc tagtttcaaa 300 aggaagaaca aaggraccct cgattgctaa cagtatgtaa aggtgcaggc ggtagcaggg 360 aggaggactg atgtgtagta gcatgaaatc tggaatgagg ttttcatgag aagccacact 420 aacttatgag tcac 434 505 423 DNA Murine 505 gaattcggcg atcccaagct tgctggttcc tttaagcagg ctgacaatcg ttctttccta 60 atgaagtggg ttaatacttt ctcctaaatt tccattgatt caaatgaaaa cttggtctgt 120 gttccagggg tgtaaactcc aaagagagtg tattaaatct gattcctatt ttgtacgttt 180 aatttctgga ctcagcacct tagaagctgt gactggctgt gttcttagca tggcaggaaa 240 tactttcagt ggatttaaaa amvctgtaga aacgatgagt agttgagtca ctacgtcttt 300 tcaaagcatg ttaaaactac ctccagaaat aggtttgcgt ttaatcaaaa agcaaacagc 360 agtttggagt taggggctga aaatgaaagg agaaaggttg agagctatga cccagcccgg 420 gcc 423 506 240 DNA Murine 506 gaattcggca gcatcatccc tcctgaggct tccgttgaca atctgcccag tcactgggtg 60 gattagacca gcttgcagaa ttccagacaa gtccataccg agagctcctt gaagtgaact 120 gatagcacca atcttagggg ygcdggcact cactgggaaa ggagatgtgg ctcctggaga 180 cccctctgag gagcaggaga ggtctatagc tgactcccca tgccagccat tgaggacaat 240 507 136 DNA Murine 507 gaattcgttt tttgagacag ggtttttctg tatagctctg gctgtcctgg aactcacttt 60 gtagaccaga ctggcctcga attcagaaat ccgcccgcct ctgtctcctg agtgctgaga 120 ttaaaggcgt gcacca 136 508 267 DNA Murine 508 gaattcggcg ccgtagccat catgaatgac acagtaacca tccggaccag gaagttcatg 60 accaaccgtc tgcttcagag gaaacagatg gtcattgatg tccttcatcc tgggaaggca 120 acagtaccaa agacagaaat tcgggaaaag ctggccaaaa tgtacaaaac cacaccagat 180 gtcatctttg tatttggatt cagaacccac ttcggtggtg gcaagaccac tggcttkggc 240 atgatctatg atyctttaga ttatgca 267 509 386 DNA Murine 509 gaattcgtgg ttgtgagcca ccatgtggtt gctgggatcc gaactcagga cctttggaag 60 agcagtcagt gctcttaacc gctgagccat ctcaccagcc cctacttgtc agatctttgg 120 aaataaaact ctctacttat ccctgaggcc attaggtttg ccagccagtg gctatacctg 180 acaagccaca gcatggtccc ttatataaca tgaaagtggg aacaaataat gagactacta 240 aagggaggaa caagataggg caatggtggc aggaaacaaa attgttccat tctctctcac 300 aagggcaatc taggtttaaa aacagtgagt atttgtgtga aaccaaaact bgagagaaga 360 agggggtcag tgagagagga aagaga 386 510 447 DNA Murine 510 gaattcgttc cttcttccac ataccgtcca aaaagaacat gcatggtccc cagaccagaa 60 gtaacaacac tgcctaaaaa cttgctagaa aaggacaatg accccacccc agatctacag 120 aatgagaaac tgtctgggtt ttaacagacc agataagtgt gctttaacaa gcttgagaac 180 ctgaagcaca ccatcctttt cagccgagaa gccacgaggg ggagtacaac ttaacagcca 240 tgggtatctg ttatgccaag gtcaaaggta gcatcctctg aggagactcc agggagtact 300 gggaacmaca ctcagaggag aaatwaccac cacagagcag gagggagaaa gagaagtagt 360 gtattaggac accaaagaga tagagtctcc caggattgat gctggcttag aagccagagc 420 aaaagatatc cmgtgttgtt atctttc 447 511 319 DNA Murine 511 gaattccata aacccaaatc tctgcccagg gtgatgggta caggcaaccc ctctttggtc 60 tccacctaac agcccctttc tcctgcagta tgaagcacat ctcctgtcct ctgctcatct 120 tgcatgccga ggatgatcca gttgtgccct ttcatctcgg tagaaagcta tacaacattg 180 ctgcaccatc tcggagtttc cgagacttca aagtccagtt tatccccttt cactcagacc 240 ttggctacag acataaatac atctacaaga gcccagagct tccaaggata ctgagggaat 300 tcctagggaa gtcgaaccc 319 512 281 DNA Murine 512 gaattctcgc attcctcctc ctccgctcgc tcttccacct ccatctcctc ctgctctgcc 60 cggtccacgt cgtggatgcc caccaggaga ctgtaatcca tgatcttcag ctgggccagg 120 aactcaacgt cccgcttcag tttttccagg aagttctttt tgctctcttc tcccacgtgc 180 agcttctgcc ctthgttgag gaagtcatta tctttgaaag ttggcaagtc cttagccttt 240 hhcttgtcac hgcttctctg gcaacagtgg aacccttcag g 281 513 301 DNA Murine 513 gaattccttt tcttttttct ttttcttcct tctaatctct ccccaggtat tcctacctga 60 ccttaacttt tcctcgggtt caagaccctt ggaaaggcct gtatacttac cgtttctcct 120 tgctcctact ctctctcccc gctttacthc ygatagactg tcctgaattt cctctagaat 180 tttcagccct atcttaagca ctatataaca wgtgaaaagg racaaaaggg cktctaacac 240 tagaaaaatt taaggccaaa cataacttgt aaagccattt tccactttac ttctgataga 300 c 301 514 391 DNA Murine 514 gaattccttt cttccttcct tccttcctcc tggccttcct cttcttcctc cttttcccct 60 tcctcctcct cttccttagc ctcaggagac ttcacgggag acttttcggc ttctggttcc 120 tcctcctttt ctcggcctct tccttctcct ctttggcgga ggctgccaac tcctctgcga 180 tggctgtgag ggtttcttcc atttctgact tctcatcttc cmctttagtt tcttcgatga 240 tctcctccac aaatttgtgt tggaccttga gcttgggggc ctcgactttg gtcttctgaa 300 tcttactgga tattgtgact gagggctgtc ggtgtgtgta cagargcccg gtgatgcttc 360 ctgaaaatgt gctaaatctg gtctcttccc c 391 515 246 DNA Murine 515 gaattcccgg ctcgagcggc cccttttttt tgggggggag acgggggctc agggtgtgaa 60 catgaggtga gacctggcat ggcagggctg agtcgtgcct gctgtcagcc cctctctgtc 120 cttcccgagg ctgagggggr actcaagctc ccttccccag cagagcccac ccacccaccc 180 hgccttcaaa gccccctttg gagagttaac tgtccgtgtg aggcgctcac tcaaccaata 240 agcccc 246 516 439 DNA Murine 516 gaattcgtat ttaaaatgac cacttcaatg caggaacctg ccgtgccagg cacttagcat 60 gctgggcatt tggctctcag cttgtccaga cgctacagca gcagcagcac aagtctcagg 120 atcatcatga ggctgagtca caggaagagg aagacagagg gacagtcacg ctgatggaca 180 ggcctgctgt gtactgccct gtcatgtccc tgtgctgtgg gctctgaggg ctctgtcacm 240 gcccttctca gaggaagcaa gggggactca ttttactgtg tcccaacttc ccagatgcaa 300 cttgaaaata ttcccttaar vvtgcaacta gaccagcagg cattactttc ttggacctct 360 taaatctcac amccattatg gtggccagga agaaactgta aacaatgaca ctttgacatc 420 ccgttgtcat tggagacac 439 517 415 DNA Murine 517 gaattcgtaa tccactaata tttatgggtg ttatcacaag tataacaata agatggtcaa 60 ctacaaaaaa caataaaaca gttgcccaaa tagcagcgta cccctacgtt agcacagcca 120 ggtataaaga tccgtagcca caccaaactc tacaactgac tgttaagtgg cataacagta 180 aatagaggaa caacccatgt tcagggatta gtgagagggt ccagatgtta gaagctgcrc 240 ctcctcccca ctccttgtac tcactccatc acttaatgca actaaagcgt gttcttcttt 300 ccttttchct cctatctgac aatgtatgct gatattaatt tgaagvcaat agccccaact 360 gccttgaaaa caaagaagta ttatgagttg tttgaacaca tgggkattaa aaaac 415 518 61 DNA Murine 518 gaattcgcgc gctgtcttcc cgctcgcgtc agggacctgc ccgactcagc ggccgccatg 60 g 61 519 393 DNA Murine 519 gaattcttct cgcgtgcgtc tcacaataca gctccccctc cacgaagaag tagcctttct 60 gcttgaggtt gaggttacag tcggcacaca caaagcactc ggggtgccgg tacttatccc 120 gggccttgac gacagcacct acaataccac tcccacactt gtcacagagc ggcatcctct 180 gggcactgcc agccccaccg tggactttcg taaccggagc cctcacgctt cgagttccag 240 ccggacggtc atcaggcccg tcattcacca gatcctgcag caccctgaag gagcccgact 300 ggcgaggagc vgctgggtca tcccggttgt catggagcat cggtacacgt ccgactgagg 360 gggcactgaa gcygtggggt cattttgcag tga 393 520 434 DNA Murine 520 gaattcggtt tgaatatgct tggcccatgt gaagtggcac tattaggata tgtggccttg 60 ttggagtagt tgtggctttg ttgtaggaag tgcatcactt tgggggtgtg ctttgaagct 120 ccgcrcagtg ggaaagagac cctcctagct gcaggggcga aagtttgttc ctggcttcct 180 ttggatgaag atgtaaaatt ctcagcccct tcadcgccat gcctgcctag atgctgctgt 240 gagtcctgcc atgatgataa tagactaaac ctcagaaccg ataagccagt atcaattaaa 300 tgttgtcctt tataagagth gcctcagtca tggtatctgt tcactgcaat gaaaccctaa 360 gtaagacact aacagaaact ataatcattt gaggagaacc acaattgaga aaatgcctcc 420 ataaaactgg tgtg 434 521 300 DNA Murine 521 gaattcgaga gaacgaacta cccagcagct caggtcagtc acctttcccc atcccctacc 60 cctgcctgca ggtttgttcc attgtgctga ggaatgtccc tgcctctggg atgacatcca 120 ggtggtataa atggaaaagt gacaaattat tcctttgctc tagtgtaggc attgctgtaa 180 ttagtagcaa gttggaacct taggaaaaaa aaatctcacc ggagtgtgaa gatgcattct 240 aatcctcagt ctgcagagta aataaagtgt cacaccagta gcctdcccga ggccacttct 300 522 495 DNA Murine 522 gattcaacac tcctcgtccc cattctaatc gccatagcct tcctaacatt agtagaacgc 60 aaaatcttag ggtacataca actacgaaaa ggccctaaca ttgttggtcc atacggcatt 120 ttacaaccat ttgcagacgc cataaaatta tttataaaag aaccaatacs ccctttaaca 180 acctctatat ccttatttat tattgcacct accctatcac tcacactagc attaagtcta 240 tgagttcccc taccaatacc acacccatta attaatttaa acctagggat tttatttatt 300 ttagcaacat ctagcctatc agtttactcc attctatgat caggatgagc ctcaaactcc 360 aaatactcac tattcggagc tttacvagcc gtagcccaaa caatttcata tgaagtaacc 420 atagctatta tccttttatc agttctatta ataaatggat cctactctct acaaacactt 480 attacaaccc aagac 495 523 393 DNA Murine 523 gaattcgttt ttgtactgtt aacattaaca attttttttt ttttaattca aaagattcca 60 ggctttcttg acactatctt tactctttat atactcagga ggtggtgctc caagggcaaa 120 gaatattaca acwgacttag ccaatttaac tgctccagct gggaatacac tctaaacaga 180 acccctacaa tcagagtcct atggctctct ctgaagagca atgtaaatca aacattagca 240 catttctatt acctgcttaa atgttcgaag tctatccagt gtcctctgtc tctcttggct 300 aacccaggca ctttttcttt cctcttcatc atgcaatttg tctctcttta tttgtattgt 360 atgatgggct ctatattcat cttcactctg aaa 393 524 244 DNA Murine 524 gaattcgtgg gtcagaagca gctttcatgt tagttcttga tttctacctt actgagtttm 60 ctgttattat actacatact ccagactagc tggacccttg agcttctggc cagctcctct 120 gtgtctaccc caaccatgct gtacgagtac tgagattaca tacttgcatc attgcacctg 180 gcttctcact cggttctgga gwtcaaactt gggttaccgg cttgcagtag caaatgtttt 240 tacc 244 525 164 DNA Murine 525 gaattcgcta tttatatata agcgataata tgggtttgta acattagttt taaaaaaggg 60 aaagttttgt tctgtatatt ttgttacctt ttacagaata aaagaattca acattaagaa 120 ccatgtaacc gagacacttg atctgacaca ggggcmgtcg ggaa 164 526 149 DNA Murine 526 gaattcttag gaagttaaaa aaaaatagtt ttgtaattaa agtataaaca aacataggca 60 atgcacacct tgtcaatcac tggagtagga tcattggatt caaatcataa tgtggatagg 120 atagggagga tgaattacca ggattcatg 149 527 59 DNA Murine 527 gaattcgctc tcttctgggt ctctgagggc gggcactgck ctcacacgtg ggcacacac 59 528 194 DNA Murine 528 gaatcchtat ttaaaaaaga ttggtcctca agatgttcat tcaaattatt cttacataca 60 cgactctgaa actttccaca actgcatttt tacctaaaaa tcatcataaa ccattcaatt 120 aagctaaatt aacyggtctc hgtagaaatg ctacaaatac aaaatactac ctagtcygat 180 tttacaaatc aaat 194 529 319 DNA Murine 529 gaattcccca tgttgtgata atttatccat gcatagctta ctatggcagc tttttgtatg 60 tggtaccatt taccacttac tttttttatt ttatgtatat gagtacacta tagcagtctt 120 caaacacccc agaagagggc atcagatccc attacagatg gttkcagcca ccatgcgttc 180 gggacctctg gaagaacagt cagtccctta actgctgagt catctctcca gcccctggtt 240 ctcactctta agaaaaaaaa gcagtagtct tagtatcaac tgtgaaaaag gtagatgtgg 300 ttagtagtat tacygaaac 319 530 278 DNA Murine 530 gaattcggat ttttaaaatt atgtgtattt gtgtgtgtcc ctatgaatgt aggtgcctat 60 agaggccgga ggtattgcat gtcctggcct gacagagcgt tgtttgtgac cggctagacg 120 taggtgccat ggcttgtaga agaacaggat ggtcttgtct ctgtctccag ctccttatta 180 atctatgagg gctctatctg catgaacacc tacatgccag arrrgggcat cagatcccat 240 tacaggtggt tgtragccac catgtggttr ctgggagt 278 531 103 DNA Murine 531 gaattcgaac cctctatcta ctatcggagc ctgagcggga atagtgggta ctgcactaag 60 tattttmacg agcagaatta ggtcaaccgg tgccttttgg aga 103 532 299 DNA Murine 532 gaattcccca gtcaaagttt gtaaatggga tccccatgag aatgacttcm gtggagcaac 60 cgagagaygc agaattccaa ccccactcta gacttactgg mtcagagtct tcataggctc 120 agcccagtga cccctgaatg tagctgtgtc tgagggaggc tgttttmcca actcttacvc 180 tccctcagtt ggscagsctt ttttacattc ttgacttcta atcccccata tggagacctc 240 caccgcctac atttctagga tgcctttcct cagtttcttt aaaaaaacaa caaaaaaac 299 533 289 DNA Murine 533 gaattcgtga tacctggctc ctaggtgacg accctcaggc gtctgaatac tttcttctct 60 ttattacaca ggcccacatt cacaattacc gttggtagca gacgagacta gatcttcgag 120 cccctgacaa catacatact tcaaagctag cagaatgaag atrcvaaatg actgtgtcat 180 aaaagtatct tctgtcatcc tgatgataaa gcattccttc aactcatagt tcctatttat 240 gtatagagcc taactccttc actgcctctt tgttctataa aagtccagg 289 534 305 DNA Murine 534 gaattcccgg cccagcdccg cttttttttt ttttttyctc taggattttg acattgctgg 60 tgagtttkac ccaatgatcc ctgatgcaga gtgtttgagg atcatgtgtg aaatcctaag 120 tggactgcag ctgggggact ttctcattaa ggtgaggcta gtcttgtaca taataaagga 180 gaagtttgaa tttkgcctgt gaaattgtct tagtattgat ttaatgagtc aagaaattta 240 gagatggcca ttgttttgag ggaadggcat tgattgccaa ggacataggt taattatatt 300 grgtt 305 535 290 DNA Murine 535 gaattcgtta tcaaagtgac acagcccaca ggggacagag aaggcccaag gactctccaa 60 atttcaagtg catgaacagt cagcacactg ataacagcaa gcctctaagg gatttggtaa 120 cctcactgcc tgatcagcta caaaaactgg acagagattt gattatggta cagagcagca 180 tatttgggtg acataaaaat gtcaccaagt gdaagcaatt agagcatccc aacctaaatc 240 catttgcaag tcctaagaat ctacatgaga agactattga aaaatatttc 290 536 168 DNA Murine 536 gaattcctcc aatctmcacc tatacttmaa aatcatgaat ctgactagcc atgccattga 60 aaaccactca gtactagagg atgaaccagt tttcaatgtt atcagccctg gaaaaccgcc 120 cagctcccdc ccccagcaca ttctattttg ttttaacatt ttataaat 168 537 275 DNA Murine 537 gaattcgagg aatatcaact tagtgctatt ttcacatcgt tcagtcaaac ttagccagag 60 ttccaacccc tacttaaaat tcaactagaa agttacctac caagtactaa ttagcattat 120 aamgtcagag cctgcagctc caggcctttc agttagttgt ttactagaaa ggacagtctt 180 aagccagata cagtttctca taagaaagtt aaagaatcca gtgaagcaag ttttttcttt 240 agccctagat tcccggcaga ctattgagca tagat 275 538 113 DNA Murine 538 gaattcctgg cttggtccag ctgccttttc ttctchtctg ttcttcctcc tcctcttcct 60 cctcacttcc cttggctgct tttccattca gagaagctgg agtccattgg cct 113 539 220 DNA Murine 539 gaattcgtaa atggcactgt aaaagggcat ttatcaacat aacaatgtaa cacctaacag 60 aaaagtgtga attcgggatc agaaaaattc aacgtttaat ttgttaaact taaagctgtc 120 actggatata gaaaaataaa ttaacttaga ttactttaaa gatctactgt cagttaaacc 180 tccacatatt ttttttaata atttaaccag cttgtctaaa 220 540 156 DNA Murine 540 gaattcccaa agtgggagga atgttaacac ygcgatagac accaagaaag agagttgggg 60 gctagagaga ggctcagtgg ttaagagcac gactactctt ccagaggtcc tgagttcaat 120 tcccagcaac cacatggtgc tcacaaccat ctgtaa 156 541 187 DNA Murine 541 gaattctgca tatcacatag ttaatccaag tccatgacca ttaachsghc cctchhmctc 60 cttctaacat caggtctagt aatatgattt cactataatt caattacmct ataacccccg 120 cctacwcacc aatatccyca caatatatca atgatgacga gacgtaattc gtgaaggaac 180 ctaccaa 187 542 92 DNA Murine 542 gaattcgatc ctttgagcca tacaacgtgt tttcgcttta aaacaaagca gacactaata 60 aaccaccgta tagataaagg atagaagaat tt 92 543 104 DNA Murine 543 gaattcctgg cttttttttt tcttcaattt cttcgtcatc atcgtcatcc tcggaatcac 60 tccaggdcwc gtaattatyc tgattcctgt tattgtcact caac 104 544 366 DNA Murine 544 gaattcgcgg tctcagggct tgtaggctgt tttatgattc atgtttcaag atgctgaagt 60 taggttccta tgtcaggaaa tcgtaggtgc acctgaattc tgtgaacagg atgtcttgtg 120 gacttcagac cttagcctaa gcttgtgttg aaaaacatgt cccccgttgg aaaaatgcta 180 tgtctgggga tctttaccca aaggacctaa gttacattta tttagttttt tcttgagaca 240 gcttaggttg gtctttaact tgcagcagtc ctcatacttt ggctctttca tgctggggtt 300 aaagtgtgtc tcatcaggct cagacatatt cttgggaggt aggaaagaaa gcatgsggca 360 gagaac 366 545 447 DNA Murine 545 gaattcggag cacttaccat ctgccctcag gaatatacct gctgcaccac agaaatggaa 60 gacaagctga gtcaacagag taaactggag tttgaaaacc ttgtagaaga gacaagccac 120 tttgtgagga ccacgtttgt gtcgaggcac aagaaatttg atgagttttt ccgagagctg 180 ctggaaaacs cagaaaagtc cctaaatgac atgtttgtcc ggacctacgg gatgctgtac 240 atgcagaatt cagaggtatt ccaggacctc ttcactgagc taaavcggta ctacacaggg 300 ggtaacgtca acctggaaga gatgctcaat gacttctggr ctcggctcct ggagaggatg 360 ttccagctga ttaaccccca gtatcacttc agcvaggact acctggagtg tgtaavcaag 420 tacacagacc agcgaagcat ttggaga 447 546 372 DNA Murine 546 gaattcatca gaggttgatg taacccctgg tttagctaaa tttttccgtt tagattcaac 60 ttctttcttc ccttctttct tatctggttc ttttcttggc ttctcttctt ccttttggcc 120 ttcttcctct tttttaagct gctttttagg ttgtttctcc tctggtccct tttttttact 180 tttatcttca tcaataacca tgtcaccgtc tgaaggacaa ggctgcttta ccactttagg 240 tctgcctctt ggtttgggaa tcttgacttc agtagctgca ggtcgtcctc tcttaggact 300 tgctttcaca ttagaagcgg ttgctgcagt caccattccc gcctcttcag tgtctacttg 360 tttttcagcc tt 372 547 372 DNA Murine 547 gaattctttt tttttccctt ttttaatttt ccacaggccc tctgtgtttg agactgtgcc 60 cactagtctg aaggttgaga ggattatttc gattggcaat taagacacaa ggggcacctg 120 gtgggcacag cgcccaccta ctcttccata tgcagttgtc tgcataattg tgcaaatgag 180 aaaaaaaaga tttattcaca agaagaaatg tgtagcgtgt agagatggct taatttgagt 240 tcctcgggca ggccggctcs ctgggggctt tcttcatctt ccctactgac ccccatcaca 300 aagggatgaa gatgcccaga tgccagggaa gggctgcttg gtccctggca gggccactga 360 gccccgtcac gg 372 548 313 DNA Murine 548 gaattcggca tgaccagtgt cattgggcct gtgagatgac caagagtccc cagagtcctg 60 gggatagaga gccctccatc ctgggagtgg aaaccttatg gtgtgttatc tagttagcag 120 gaaatgttag agaccacagt agggacaggt gaaagtctgt tgcctcacag ggtctgacac 180 tgatggagca gattgtgtca acaatgtgtc acaggaatgg aaagaatgtg ccctgagccc 240 acctccccac cccaccccaa aaaaccccat aaaaccaaaa atcaaataaa tgaataaaca 300 cacacacaca cac 313 549 283 DNA Murine 549 gaattcattg ccttgagata gggtctcaag ttgaatttag aagtacgtat tggataggct 60 aaccacgcag ttcttttgat ctctacctgg kcccaacgtt aaggtgtagg ccagctcagc 120 catgcctggc tttttcatgg gcacagggag attcaagccc tcatgcttac acagcaagca 180 cctgtagaat tttaatccag caacatggct gctccagcga gggatcacat ccaaaggcct 240 tctaggtcta tgtgatccgb ctggagaatt ccaccacact ggc 283 550 342 DNA Murine 550 gaattccttc agaagagtca tttacatttt tcttatttta taaaaataat agtttaaaaa 60 aaaaccaaac cacaacaaaa atcacatgtt cacagtagag ggttactgtt aggttttaac 120 actgttcttc atgccgtttc tgcagcgtaa sagcaaacaa atccacaaac ttagacaccc 180 atatcttggg ggctggagtg atgctcagca gttaagagca ctgacagctt ttwgtcctga 240 gttcaaatcc cagcaatcac atggtggttc atgaccatcc gtaatgagat ctgaccccct 300 tttgtggtgt gtctgaagac agctatagtg tacttacacc ca 342 551 373 DNA Murine 551 gaattcggcg ccttccttta gacgcatccc ccgggcccct gaggagtcag cccgctcact 60 cccggcgagg tccaccaagc tgatcttact gaccttttct gaatccaggc cagtaagctg 120 gtcatgggat cgctgggtaa agacaatagt aaagacggca tgggagcggc tgctggtctc 180 gttcatgttg gtggcagcca cggttcttgc cttatttcca cagtccatga ggtcggcaat 240 gtctgcatag gaagtcacag ccagtttaga caggtcttgc acgtacgggc ctaggatggg 300 gtgctcccgg acccgcagag agccccgact cttgggggtt caagaggtct cgtacycctc 360 gcaatagatt tcc 373 552 474 DNA Murine 552 gaattcgaag aagatgatga tgatgaataa gttggttcta gcgcagtttt tttttcttgt 60 ctataaagca tttaaccccc ctgtacacaa ctcactcctt ttaaagaaaa aaattgaaat 120 gtaaggctgt gtaagatttg tttttaaact gtacagtgtc tttttttgta tagttaacac 180 actaccgaat gtgtctttag atagccctgt cctggtggta ttttcaatag ccactaacct 240 tgcctggtac agtctggggg ttgtaaattg gcatggaaat ttaaagcagg ttcttgttgg 300 tgcacagcac aaattagtta tatatgggga cagtagtttg gttttttgtt ttgttttttt 360 ttttttttct tttggttttc ttttttgggt tttatttttt ttcatcttca gttgtctctg 420 atgcagctta tacgaagata attgttgttc tgttaactga ataccactct gtaa 474 553 500 DNA Murine 553 gaattcaaac tagaacccaa gtcacagcat tttcccacat aactctgagg ccatggccca 60 tccacagcct cctggtcccc tgcactaccc agtgtctcac tggctgtgtt ggaaacggag 120 ttgcataagc tcaccgtcca caagcacgag gagatatctc tagctttcat ttctgttttg 180 catttgactc ttaacactca cccagactct gtgcttattt cattttgggg gatgtgggct 240 ttttcccctg gtggtttgga gttaggcaga gggaagttac agacacaggt acaaaatttg 300 ggtaaagatg ctgtgagacc tgaggaccca ccagtcagaa cccacatggc aagtcttagt 360 agcctaggtc aaggaaagac agaataatcc agagctgtgg cacacatgac agactcccag 420 cagcccggga ccctgctgtc ttctcgactc ttagggcgtt tctttccatg tttggctgtt 480 ggktttagtt ttggtgagcc 500 554 233 DNA Murine 554 aaagtattgt gttaactcat tagtctggaa aagcaactaa aaaagtttag tgtaaataca 60 atagaatgcc atatttgttt ataaaaaagg aggtggactg tgtgactgac tgtgatacag 120 tagggtggca agggcgaggc agccatcatt acgtgtgagc agcgacctca ctgacactac 180 actgctgaac ccaaacagta gagcagcaga tgcctatcag gagacctgca cag 233 555 195 DNA Murine 555 tgccaagtag cctacactgg ctttgctgtg gccctcctac atttgtctcc tctgtgctca 60 aagtatatga gtctgttatg gatattgctg gctgtaaaac aacataaata atcactttta 120 gtgatatttt tgctatacat gttgaacaca aaagctttac atgctttgat cagtctggat 180 taattgcgat acatc 195 556 201 DNA Murine 556 gcggcccgtt tttttttttt tttttttttt ttttttagta gaaatatttt attggtgaga 60 ccccaccatc tgcacaaagt ggtcctggaa tcaagctcct tcctccttgg caatgcgatc 120 tttcttgagt ggtccataaa tgtttcttct ctcatggctg gagcgacatg caattgagtg 180 gtcatgacta gatttcaggc c 201 557 188 DNA Murine 557 ccggctcgag cggccctttt tttttttttt tttttttttt tcgtgactta caacctttta 60 ttagtgaaag tgaccatggg ttcaaataag tgtgattgct ctgctccgct cgctctggga 120 gcagtgtttc tttttttttt aattcaagat gactaaaaaa gtcactttca agtgactgtg 180 tgtctgag 188 558 227 DNA Murine 558 gttcatagaa aagtactcaa ttttttactt gcaaagcagt cctgggttaa ggtaagtttt 60 atatgtgtgc actgttacaa agtttgcttt gtagatggag agcccgatac accgtatttg 120 aaaaaaggta gaaagcagaa atgatagatt ctgataccta ggaagttaga tacagatttc 180 agtgatatgt catatgcatg gatgagagta aatactatta atatcag 227 559 90 DNA Murine 559 gttaacagca actttattat tccatgatga aaaaagttgt agttgttgat gcattcacat 60 aaattacaat agtggaggat cataaattac 90 560 199 DNA Murine 560 caggaaggct gtcccacagg aatataagtg aggcacaaat gttattttaa tatttccagt 60 atgatgtgta gggctgggga ggagggacgg gggaaatggc tcagaagata aaaaacccgt 120 caacaagcct gacaacatga gttcaatccc aggtaggaag aactgactct atagctgtct 180 ctgacatcat gtttgtcat 199 561 188 DNA Murine 561 ctggtactgt ggccctccgt gaaatcagac gctatcagaa gtccactgaa cttctgatcc 60 gcaagctccc ctttcagcgt ctggtgcgag aaattgctca ggacttcaaa acagatctgc 120 gcttcagagt gcagctattg gtgctttcga ghaggcagtt gaggctattt ggtttgaaga 180 tacaatct 188 562 174 DNA Murine 562 gaaacaggag gggtcagtct gtcagaaaaa gttgacagtg aacttaaaac tttagaacaa 60 ttatcttcat tttcttctga tgaggaagat cctggctcgt gtggccatga tatctataag 120 aacacctctg ctcccttact gtgttggatg ctacttcgat aaacaagaaa cttg 174 563 166 DNA Murine 563 ccgtctaagt gcccagcaca tgactacagc tttgtcacat cctggctcta tccaagctgt 60 ctcacctcat ctgcccacag ttcttgggct gcagaccaga ctgtttctgc aggcttgttc 120 ctgcctctct ggcttcactc ttgtaccctt ctccccaata ttctct 166 564 121 DNA Murine 564 gcaactaaaa aagtttgtgt aaatacaata gaataccata tttcgatata tataaaaaag 60 gaggcggact gcgtgactgc tgtgcatcag tcagggtggc aagggcgagg cagcatcagt 120 t 121 565 270 DNA Murine 565 aaagaaaaca ttgtttctta atttgtaacg ttaaagtctc ctggaactcc tacttctaat 60 gaaaattgca aattagatag agagaaagag agagagaatg aatacatcta tcaatagaac 120 cttgtacatt tatcatgtat aaggctatca atcatatctg aggctagact cttagaatta 180 ctctgagcct attctcctct cggcatgaca ctgatgcaca tatacatagc tgtctacttc 240 ttctagctac tgacttatat atatatgtgt 270 566 156 DNA Murine 566 ggtgagcagc gctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg 60 tcggctctcg gcaccgaatg cgtatgattc tccgccagca tcgttcggca gtgcgtcgac 120 agacgccgct tgttctgaag tgcagtaaag cgccgg 156 567 231 DNA Murine 567 ccaactaaag gaactgcctg aaaaaatgcc cagaactctc caggaacttc gtgtccatga 60 gaatgaggat caccaagctg cggaaatccg acttcaatgg actgaacaat gtgcttgtca 120 tagaactggg cggcaaccca ctgaaaaaac tctgggattg aaaacggagc cttcaggact 180 gaagagtctc tcatactcgc atctcagaca ccaacataac tgcgatccta g 231 568 206 DNA Murine 568 cagtgctaac aggtccatga ctgggtccag gtcctgcctg ggctgctcag cgaagagttc 60 gccgatggct cgtaggcgtc tccagtgaag gcaatggctg attcagaccc acggagaagg 120 cctgctgatc cagctcaaag gcttggccga gaccccggaa gactccccac cttctgatac 180 tccttcttga aagccagtca cctctt 206 569 262 DNA Murine 569 ggagatggct tagtggataa gagtacttct atgcaagcat gaggacataa cctcagtaaa 60 aggctgagca tatccgtgtg tacctataac aagtatctgc agttctcagg aactctctgg 120 gtaatcaggc taactaaaac agtgaactgc cacttcagtg agagaccctg tctcaaggca 180 acaagacaga tagtaataga gggagacacc aatgtctctg tgttcaaaca cacacatatg 240 gaggcatgtg gttaaatgta ct 262 570 219 DNA Murine 570 cagcgacaga cggacagact ctcgggtggt cacactcacg ataaaagctg gcaggctgac 60 agaggcaacc tcaggacgga ctttctggct actgaccatt ttcctgtgtc ttactaggat 120 cgtgtgtgga cgtgagatca ccatgagctc cgttgacagt tttgacccaa gagagttttt 180 ctgaacatcg aagtgggctg gttccacaac aaatcaagt 219 571 167 DNA Murine 571 gtggacaaag cgttcccatc gcttacggga gtgtctgccc aagatatcgt tgaaacgtgg 60 atctaattca atgttgtact tgtcaatata gtcatataaa tcttctgttc ccagaacctt 120 ggctatcctc accaacctga tcataattgg ctcatgtcca tggaaaa 167 572 230 DNA Murine 572 cagctctcca ccattgagct ggacagctgc tgtgacccag gctgctgaga acgccacctc 60 agctctgttg agggagcagg aaggctcacg tccagctccc ctcaggcaca gatctcctgg 120 caatgaaagc gccatctctc cagcaagccg tggagatgcg gctgaagatc aggttcataa 180 gcttcggctc aaacttctta aaaattaaag gcaaaaagaa gaaactagct 230 573 237 DNA Murine 573 cgctcgcgtc tgtccttaag gctctcctcg gtgtccacgg ctccctcttt ccttgctttg 60 cagcgatcct actgccagaa attcgccatg tctattctca ggatccacgc cagagagatc 120 tttgactccc gtggaatcca ctgttgaggt cgatctgtac accgcaaaag gtctcttgag 180 ctgcggtgcc cagcgtgctc actgactcta cagcctagaa ctcgagacat gataaga 237 574 231 DNA Murine 574 gatccacttg gatggccgca cgttttacat tgaccataat agcaaaatta cccagtggaa 60 gatccaagac tacagaaccc agccatcact ggtccggctg ttccgtactc cagagagttt 120 aagcagaaat acgactactt taggaagaaa ttaaagaagc ctgctgatat tcaaacggtt 180 tgaaatgaaa cttacacgaa acaacatatt tgaagagtct atgcaggatc a 231 575 143 DNA Murine 575 atgaatttgt ttggttggtt ttgtttttga gacagggttt ctctgtgcaa cagccctggc 60 tatcctggaa ctcactatgt aaataaacta agctaagctg gccttgaact cacagtgaca 120 ggcctttaat ctcagcactc aag 143 576 113 DNA Murine 576 ccatattgaa ttagatatct tatttcagga catccatgtc aaaataaaac aaaagagtca 60 atccttgcaa caataatgtg tattcattaa aacgcatttc acaatcatcc cat 113 577 168 DNA Murine 577 gctttggtaa atgtggcact aaatcttagc attaattgga taacacacaa agacagtacg 60 aggcagaacg gaataaaatg attggaaaac gagctaacga aaggctagac tctgttacaa 120 agcgtaagag cttcaggaaa tcaagataga tagaaaatat gatgatgc 168 578 245 DNA Murine 578 atgaaatatg tggaaacatc agcttctcag ttttggaaat taaacagtaa gtcataaagc 60 tcagataggg cactagcttt gtagtgccat gaacagcagc atcaacataa agtttggctc 120 ttgagagcaa accaaggagc acgttgtaga cctgatgtag gaatactgtt atatctggac 180 tgagtggaag gtcacggttg ggatgtgcaa gactgtgacg acacttgcga tgatcgttgg 240 atagg 245 579 108 DNA Murine 579 gggccgtggc agagcgcgga gaggcctgcg ggtggcagcg gcgggcgggc ccgtcgggcc 60 ggagccgagc cgagccgcgc cgcgctctgc tccgagccgt aagccctt 108 580 213 DNA Murine 580 gccccccaga cctcttgaga gtcacctagc catcaatgga actccaaccg gcagagcaaa 60 tctgagtctg actactcaga tggggacaat gacagcatca acagcacctc caactccaat 120 gacaccataa actcgctcga gtctcatcac gggacagcct cggaacagac actcagcaag 180 cagacatacc acaggagacc gcacagctgg act 213 581 153 DNA Murine 581 gagcaactca ttgctgcaaa attctgtttt gctggccttg ttatagggca gactattgtg 60 gacatcatga gtcatgccac acaagctatt tttgaaattc tggagaaatc ctggctgccc 120 caggactgta ccggttgata taagattgaa ttt 153 582 155 DNA Murine 582 ctggttccct gggaggccag gagactcaga tctctggagc tagagttaca ggtggctgtg 60 agctgctaaa aagcgggaac taagccacag tcctttgtac atatcttgta cttttgcatt 120 tatacaaagt aagaaattcc tcactctctt aacag 155 583 229 DNA Murine 583 cttcccaaat atgagagggt caaggaactg tgccagcaag ccagatacca gacagcctgt 60 gagcagcctg ggcagaactg gcagtgcatc gaggacacat ccggcaagct ccgaatccac 120 aagtgtaagg gacccagcga cctgctcacg gtccgtcaga atgcacgcaa cctctactct 180 cgcggattgc atgacaaaga caaagagtgc attgtaggga ctctgctat 229 584 215 DNA Murine 584 caggatttct ttgtgtagtc ctggctgtcc tggaactcac tccgtagacc aggcttgaac 60 tcagaaatcc acctgcctct gcctcccaag tgctggaatt aaaggcgtgc gcacccctgc 120 ccattgcctg aactcttttt atgtcagttc tttgtctccc actagaaaga atgttgcagg 180 accctctccc cattgccaca aggtcagaag actct 215 585 230 DNA Murine 585 gggatatcaa aaaagtttaa aagcgaaact tgagctgcct gaaattcctg tgacaaaaga 60 tgatgtagaa gattcagact cagaagtgag tgaatttttt gatagctttg atcagtttga 120 tgaactagag caaactttgg agacttactt gctcatggaa gatcctatca tagggaagtc 180 atcacagaag atagggcaca atatgaaaac tgatgatctc agaatcagtt 230 586 212 DNA Murine 586 acgctttagt tcaggattga acggagcata cacttcttcg aaacaaagct tatttattct 60 tgagcagcca cacattggtg cactctggtg caggaactgg gaattcggga aaagtgggtg 120 tatctctggt aatggaggct gagacatgcc tggtcacctt ccaggaccat gacaggcctg 180 actaatgaga gggcaaaggg ccttgagact gg 212 587 212 DNA Murine 587 aagatttatt ttacttatga gtacactgta gctgtacagg tggttgtgag ccatcaagta 60 gttgctggga attgaactca ggacctttgc ttgctccagc cccactcatt ctggcccaaa 120 gatttattta ttgtttatgt gagtatattg tagcgtgtct tcagacacac cagaagaggg 180 attcagactc attacagatg gttgtgagca ca 212 588 193 DNA Murine 588 ctgtattgtt atttttctct cactacctcc ccgggtcgga gtgggtaatt tgcgcgcctg 60 ctgccttcct tggatgtggt agccgtttct caggtccctc tccggaatcg aaccctgatt 120 ccccgtcacc cgtggcacca tggtaggcac ggcgactacc atcgaaagtt gatagggcag 180 acctcgaatg ggt 193 589 226 DNA Murine 589 acaaaactca aagtcttcca actgatgtgg atgtcctttg atgtaaaaca ttcgtacgtt 60 atttgctatc attgctctct gcacactctg tcaccaaagc cacaggattg agtgacacat 120 ctctccaagt taaaaaatat ccattttcca ccaccaagtc tctgcaggtc tccttttgct 180 catactagcc tttcatgcct ggaccaccat catcacacag ttcaag 226 590 243 DNA Murine 590 ctctctgtta ctgttctcta tattcagatg tcactataaa atatttcaat attccaatga 60 attcctatct aaaacctaga atgcaaaaag cacacagaac aaattgccat tccttcttaa 120 aatccactct ttctgcacta acttgcttct acttcaagta aaatttgttt tcaaaagcca 180 ctgatcatat atactttaaa ttacttatac ttagagacac acagctaagt ctagatacat 240 gag 243 591 261 DNA Murine 591 ttttacagag gtgctaggaa tccaaacttt ggtccttaca ctagtgcaaa aagcactttc 60 cttgtccagt catctccctg cctttgcaca ctgcgatttt ggcacacctg accaatgcta 120 cctgtgacct agatttctga ctgctatttc cctttgttca ttttaggcca gaaacagaaa 180 cagaaccagt gcagacaggc tctacctgtc tggcagtata cacttgctat gctcacatct 240 atgcatactc agagactagt g 261 592 274 DNA Murine 592 gttcgtgtcc agtctgtatg aatgaatgtt ctatgttttg tgttggataa taaagatggt 60 ataaaaaact ttatctgcaa agccgagagc tgccacgtgt ttcagccagg aatcagacac 120 gtggcgagag ggcccctcgt ggaaaaaact gttcgtttta ggaaataggg cgagtgcaca 180 gcctctagtt cagagtaaaa gctaataaat gtctagatta atgtgttgca atgtaaggtt 240 ttattatgat gagctcaaaa tatatcctga tgct 274 593 252 DNA Murine 593 caaatactag taaacctaca cagtgtgcac ataataacag acatatttgc tttcatatgc 60 ggagtgtgta tatatttgag gttttcttct ttttttctct ttctctttcc ttctgtttct 120 ctctctgtgt ctccctctct gtctctgttt ctgtctctct cttttttgtc tcccgttcat 180 aaagtctact gtgcagttct gactggctga acttcgtatg tagacaggct gttcaaatca 240 gagatcacat ga 252 594 246 DNA Murine 594 cctataggtc tgcagaccct ttcttctcct tgggtacttt ctctagctcc ttctttgggg 60 accctgtgct ctgtccaatg gatgactgtg agtgtccact tctgtatttg ccaggcactg 120 caaagcctca caagagacgg ctatatcagg ctcctgtcag caaaagcttg ttgacatctg 180 caatagtgcc tgggttggtg gttgtttatg gatgatccga gtgtgcagtc actgatgtac 240 ttctcg 246 595 246 DNA Murine 595 ttcacaatgg tttttgcaag ttaaacagtg aaggtgaatt aaattcatac tgtcttgcag 60 acttcagggt ttcttcccca agacaaaaca ctaatctgtg tgcatattga caattcctta 120 caattatcag tcaaagaaat gccatttaaa attacaattt ttttaatccc taatggatga 180 ccactatcaa gatgtatact tgcctgtaac agtaatgatc tctatatcta gcacagtagt 240 attaaa 246 596 213 DNA Murine 596 gaagttccag tgggctttta ttgagataaa ttaacaaaaa gaaacaatca agattttacc 60 aaccatcttt tctgaatgaa ccatgtatat aactccttaa agactcaggt ccatagacat 120 gcacatacac tgtaacacat ccaacaaaac agaccctccc actggaacat tgcataacag 180 aagcatttct tccaatgttc aatttagtct act 213 597 256 DNA Murine 597 gcccacttta tgagcttctc aacccttcct gaaatttcaa tcccaaaatt ctgaattccg 60 agatcaatag gaagacattg taggaaggct caagacagaa taaagctgga ggctcagtgt 120 ccatacattc acttgagccc acactttggt gaccctctac cagctgtaaa acacaagatc 180 ctctttcctc ctgctgccag attcatgtct gacatcagaa actatcgata gactagactg 240 agtctgagac ctgaga 256 598 234 DNA Murine 598 ccagggttgt ggggacacag atgagggctg ggaggggggg aacgcaagag ggcggggggt 60 ttcttcacga tcgcactgga agattttata agagttttgg ggggggggac agtaaagctc 120 tgagccactt gggttcttca ggagtttctc ttaggagttt ctcttaggga aagttttttt 180 tttcctcttt tttaatatat aactataata tatatgaata taattgctaa tgtt 234 599 167 DNA Murine misc_feature (1)...(167) n = A,T,C or G 599 cttccctgtc agttctggag tttgtatgaa ttctctgatg tcattgcctg taacctcaag 60 ttattcctta atgtagaatg tctgcttggt actttttgtt atttgttgtt ctttgttatt 120 gatgttgttc ccttngtctc aaaagatgaa tgacctggag aaggaat 167 600 170 DNA Murine 600 cacaatgtct atagctgcaa ccctgcttcc cacagtgaag tcttcccgtt ccttatttcc 60 aaaggtagtt cagagaggtc agacatcttg cccccaaagt cctgacccat acttagccag 120 agaactaggt ccataaataa atctacttgg ccctaaagca aaatgccccc 170 601 204 DNA Murine misc_feature (1)...(204) n = A,T,C or G 601 ccggctcgag cggcnntttt tgtttgtttt ttcttttctt tncttttttt tttcctaact 60 ttttttngag gggggatgat agatttttta agtttcccct gttttcttga tatttggaat 120 tctggcctac ttcactatta ataacagtag aagcagtagg agatactggg ttgggaattt 180 gaagttggct tgagtttgag tctt 204 602 212 DNA Murine 602 ctagaactca gtcttgggtt tgaactaact ggtttgagtt aactttgctg ttaacaaaca 60 ggagtctata ctttgaggaa tatcaaagct ataaacttca gaccatttcc tttaattcac 120 aggcatccaa acaggatggc cttcaacatc atggttcaga ggtctactcc aagtatctag 180 gtctttgtaa ccagtctagt gaacaatatt tc 212 603 187 DNA Murine misc_feature (1)...(187) n = A,T,C or G 603 gcggccnttt tttttttttt cccttttgtt tgttttaaag ggcatagagt gcgattgaac 60 tttgaggggc cttctgctta ttagataagc atggtctctg tcctaaaaaa cagcatctac 120 tgtgtactga cattttagtt tctgtggacg aagtaaatgc agcatttggt ttgggggaga 180 acatttt 187 604 232 DNA Murine misc_feature (1)...(232) n = A,T,C or G 604 tctccttccc cgccaccgnt gtcagaagct catcgaggtg gatgacgagc tcanncgcac 60 cttctatgag aagcgcatgg ccacggaagt agccgctgat gctcttggtg aagagtggaa 120 gggttatgtg gtccggatca gcggtgggaa tgacaagcaa ggtttttccc atgaagcaag 180 gtgttctgac ccatggcaga gtgcgcctct gttgagtaag ggcattctgt ta 232 605 178 DNA Murine misc_feature (1)...(178) n = A,T,C or G 605 aagagtttga gacagcggag actctgctga actcggaagt ccacatgctt ctggagcatc 60 gaaagcagca gaacgagagc gcggaggacg agcaggagct gtcggaggtc ttcatgaaaa 120 ccctcaacta cacggcncgc ttcagccggt tcaaaaaaca gagagaccat tgccagtg 178 606 200 DNA Murine misc_feature (1)...(200) n = A,T,C or G 606 taaatttcaa aaaaagaaaa aggtagaaat tgaattagca agagcttaag ttttctttaa 60 acatgctggc cagggcngca gtggtggtgc atgcctttaa tcccaacact tgggaghcca 120 gaggaggcag atttctgagt ttgaggccag cctacagagt gagtttcagg acaacctggg 180 ctatataaag aaaccctgtt 200 607 173 DNA Murine 607 ggcttactag gagggtgaat acgtaggctt gaattaatgc tactgcaaat tctagaattg 60 tgagtagaag taaaataata aatgtaatgg tagctgttgg tgggctaata tttattaata 120 ctagagtagc tcctccgatt aggtgtatta ataagtgtct gcagtaatgt tag 173 608 206 DNA Murine 608 taggcccttt cctttctttt actccctagc catagggtga gtctcctgca ggttgattcc 60 tgcaggttgt tctctcactc ctgcagtgtg catgtcctgg tgtgtttata cacacataca 120 tacatcatgc accatacata tacatacaca catacataca tatatgcaca cacatacatg 180 tgatgcatac aaaattttct ttaatt 206 609 257 DNA Murine 609 ctttactact gagtcaaact tccagcctct agtcttaata taaagaacat tgtttcttgt 60 gttaacacag aatattgata gttctaagtc agatttatca tgttcaaatt tttatattag 120 ttaattatgg aaaaagaatg ggaagggctg taagaaacac taaatccaca gacaccttaa 180 aatactatga tagtaatttc atcaaatggc cagtgtggcc atattagaga aaagcagtaa 240 attggagagt acaagag 257 610 246 DNA Murine 610 atgggcacta cttgaggttg tatataaaca aaaatgacac gaggaaactc ttgatttcag 60 tttcaaaggg gagaactaca tgtactacag acaaggacga gagggtgaaa gagcagatct 120 ttagcatcaa ggactgaatg gcactggtgc tgccaacata tggaagtgtg gatagctgaa 180 cagaagtgag cagctgccga gccagatgca aatgatgttg ttcttccaga gtgcaaggat 240 gagtcg 246 611 178 DNA Murine 611 ggcccatttc ttaggcttgt gttttagcaa agtatacctg cgtggccatc ttgtccacgc 60 caatgcagag gtcctaaaag gactccctct attctctatc cctgtggacg taaagacact 120 ggcatctctg ttaccttctc ttccctttgc aagggtttac ttggatcttc agagaaag 178 612 218 DNA Murine 612 cactttttat ttttgttttt ttacagtgag atttttttga cttcagctac accatcttcc 60 tactgtttcc cttgaaatcc catcctgctt ttcctgtaca ctacccctca caaaccacaa 120 gccgcagcaa catggatgcc cagtctggag cagcaacagc caggatgacc tggagccagg 180 ggggccttcg gaacagatgt ataccttctg gtgagttt 218 613 238 DNA Murine 613 cattcttcat gtctctaaac ctttttttta aacaccttgg gggaggttgt attctggcat 60 tttaaataaa aataagatgc ttgatgccag aatgaaataa tagaaataat gcctcctgtc 120 cctgacccat gattcagagt accttttccc tggcaaagta ccctggtaac attttaaaac 180 acacctaaca tgtcaacatg tcaatatgcc atcaaaaacc cacaaattaa tcgatttt 238 614 214 DNA Murine 614 tcctcttcat atttgtcttc cttctgagag tacttctcag cctgagcctc cagtgattca 60 agttgttcgt caccgttttc aattcttctt caagctcggc acatttgcct tctgagagct 120 cagcccgctc ctctgcacgt tccaggtcgc tctcgatgat gaccagctta gggccacctc 180 ttcatacttc cggtcagcat cttcagcaat gtgc 214 615 154 DNA Murine 615 attttaggga aaatgggatt gactctctga actcaacaaa actggaattt tttttttccc 60 cagaagcgag aaatgaaaag agaagggcct aaggaaagca gaaggcggcc tgaagtgaca 120 atacctttaa aaaactctta tctctgtgtg gggg 154 616 106 DNA Murine 616 cgggagggcg gcgcggcacg ggcccggtcg ctcccgccgc agctgctggc ccgcacgctg 60 ttcctgacag ctgggccttg gcgctctcgt ctcagccgcg tcccgg 106 617 240 DNA Murine 617 cactcttctg acttagaggt tcagcttgat gctaacatga aaccaatgcc ctttaatagt 60 gaagcgacac caactgaaga tggagctcaa ttacggttta agcaagtagg agtcagcctt 120 acagatgatt tgatgaatca gttgctgaag ggaaaagcca agaggtattt ccaggggcaa 180 attgagttag agactggcca gccacccatg gagttaagaa gaagacaact gtaccttgtg 240 618 244 DNA Murine 618 tttgaaagtg aaaagacttt tattccacat ttggagccct tacagaggaa catggatgga 60 gagctacagg tggttcactg tgacttcttt aaaatggatc ctagatatca ggaagtagta 120 agaccagatg tgagttcaca ggcaatattt cagaacctgg gaataaaaga gttccttttc 180 agcaggtgtt cctataaagt attggaatcc taccatataa actgaagacg atactttgaa 240 attc 244 619 257 DNA Murine 619 ccaggaactg tccagtgaag agataaagtc ccgtgtttga aactttaaga acttttaaaa 60 taaagactgg aaatgggaaa actgatagaa tttaaaatca acagaatgta ttcctttgac 120 aattctcccc atagctttat tcctagcact caaggtctag gcaggaggtc tgtcgtaagc 180 ttcaaggcag cctgtactat acacggaatt cagattacca caatgagctt ctatctcaaa 240 cacataagct ttctttc 257 620 243 DNA Murine 620 tttttataag actggttctc actgtagctc tggctggcct gaaactcact atgtaaaacc 60 agatgcagag gacaacaggc tggtcttgaa ctaagggacc atcctgcctc tgcctcccaa 120 aggctggatt acaggtgggt gccaccacac ctggtttaaa tcgagactaa aaaactgttc 180 tgtcttttag gtaatccaat tattcagaat agacctcaag tctctaaaga ggattttgat 240 ctt 243 621 219 DNA Murine 621 gatggggaga gtcacatgag tccccttctc cacctttgcc tcagtaatct tttccatact 60 ctctgacgag gcatgagggc agaccttagc ctttaaagcg ccacggttca tttatgtgtt 120 gaaaagaaag tacttgcgta cttgtgtctt ggctcctcag cctgcttcca caccagctga 180 cagtgggtac gtgagccagg ctgctggaga ggcatatgg 219 622 224 DNA Murine 622 ttggattaga atatacactc tgaaaacctg cagcgtggct cggtgcctgc tgccgcatct 60 gaaaccctga agaaaatctc tggtgggaaa cagatggtgg aagaagaaaa aagtgtgtgt 120 gtgtgtgtgt gtgtgtgttc tctgagtttt ggttggagga ggtacttcac agcacttgat 180 ctagcctggc cactgagaag cggggatttt actcaaaggt cgtc 224 623 194 DNA Murine 623 ggaagccagc aggaacagta ggacagtcgt caggctgtga ggtgggtaag aaatacagaa 60 atgctaagta aggatatact cctcctagca ggttgcctaa ggaggtaaat ggtggtggtc 120 tgatctggtg ggttctagtg aactaggcca agagctacat gagatctgag gggaagttgt 180 aataccagca gggg 194 624 195 DNA Murine 624 gaaggattct gggaaagttc caggccccat gaagttaatt ccctggctgg gatagtcgct 60 gggggttggg gccgaagggg ctcgaatggg gcaaaagggg cagccagggc ccagggctgc 120 aatcatctcc atcacactgg gcatgagcac atgggcaggg ctcacagtgc ggcacgcttc 180 agcactggcc catcc 195 625 257 DNA Murine 625 ggccgttggt tgtgtttgga tatacgactg ctatagctac tgaggaatat ccagagactt 60 ggggatctaa ctgattaatt ttgggttttt tagtattggg ggtgattata gaggtttttt 120 taatttgtgt gcttaattat tatgatgaag tggagtaatt aatcttgatg gtttgggaga 180 ttggttgatg tatgaggttg atgatgttgg agttatgttg gaaggaggga ttggggtagc 240 gcaatatata gttgtgc 257 626 95 DNA Murine 626 aagcaagttt aaaaactgct ttattgattt gaagtaccaa atttataaag attataacag 60 tttgcatcta ctcaaagtta aataatttac attgg 95 627 194 DNA Murine 627 gtgggagact ttatttatcc agtgtggtga tagcatggcc ctccatgctt tttactggtt 60 aatgctattt ctcacaatga tgcagattag gaaaattgaa gtattcagga aacaggggtg 120 gttgctaagg ctccgtacct gctctctata aaattatagt ggctttgacc tgacatagga 180 aagttcaagt ctag 194 628 176 DNA Murine misc_feature (1)...(176) n = A,T,C or G 628 tttagtttgt gtcggaagcc tgtaattacn gctccagctc atagtggaat ggctatactt 60 agatttatgg atagttgggt agtaggtgta aatgtatgtg gtaaaaggcc taggagattt 120 gttgatccaa taaatatgat tagggaaaca attattaggg tcatgttcgt cttttt 176 629 202 DNA Murine 629 ttggtcacag ccttctcagc agcagcctgc tcctccttct caatctcctc tgggtctctg 60 tagaagtaaa gatcaggcat gacctcccag gggtgctcac gggagatagt acctcgcatg 120 cggagtactt ccctggccag catccaccac atcagaccca ctgagtgagc tccttgttgt 180 tgcgaattcc accacatggc gg 202 630 243 DNA Murine 630 gttactactc tccaggttat gcacagtcca gcccagggac tctcacctca agcaaccagg 60 caggaatgga gggccagccc ctaaagacaa aaaaggatga ggagcctgag agcgtagaag 120 ggaaagtaaa gaatgacgtc tgtgaggaaa agaagccgga gctgagcaat tccagtcagc 180 agcctccgtc atcagcagcg gccaacatgt acatgcagtc ctgtactaca ccagtagtct 240 acg 243 631 266 DNA Murine 631 aaaacataat aaatgatctt agtgataagc taaaaagtac aatgcagcag caagagcggg 60 ataaagattt gatagagtcg ctctctgagg accgagctcg tttgcttgaa gagaagaagc 120 agcttgaaga ggaagtgagt aaactccgca ctagcagttt tctttcctca gcacctgtgg 180 ctgcagccca gagctctatg gtgcgtgtgc atgagctcca gggcagcaga gagatcatca 240 tggagacgca gatgaaggag actgat 266 632 234 DNA Murine 632 cccaggacca gatgggttta gtgcagagtt ctatcagacc ttcaaagaag atctaatccc 60 ggttcttcac aaactattcc acaaaataga agtagaaggt actgtaccca actcattctc 120 gaagccacaa ttactctgat acctaaacca caaaaagacg caacaaagag aacttcagac 180 caatttcctt atgaatatcg atgcaaatgc tcaataagtt ctactaacga tcag 234 633 204 DNA Murine 633 gatttttttt tttttttttt tttttttaat tctttttttt ttccttcttt cctctttttt 60 tcctctctct cctcctaata cacacttttt ttagtaaggg gaataccatg atgtcgctct 120 agcccggccc ctgtagattc gaccccgggg cctgctgtta aaaccactgt agaatcgaga 180 cggagctgtt gtagttggta gtcc 204 634 205 DNA Murine 634 gaaatgattg cagtccacct ccgtacgtaa cactcgtgtt ttaccgaagt tatcacttca 60 caaaagctag agtatgggtt ttaagtaagc agggacattc atgctttcat ctttgcaaaa 120 tcttgtgaaa ctaggaatga agtctaaggg gtatagacga gtcctcataa accgcagaga 180 tagcgtaacc ccatatgaca caagg 205 635 227 DNA Murine 635 gaattcgtaa aattacacat gcaaacctcc atagaccggt gtaaaatccc ttaaacattt 60 acttaaaatt taaggagagg gtatcaagca cattaaaata gcttaagaca ccttgcctag 120 ccacaccccc acggactcag cagtgataaa tattaagcaa taaacgaaag tttgactaag 180 ttatacctct tagggttggt aaatttcgtg ccagccaccg cgtcata 227 636 218 DNA Murine 636 ggttttccta catcttacaa tggactaaga aaaacatcac atatgtgtcc tcattccttt 60 tcatcttaca cctaattagg gagacaccaa tgcccatgga aaggctgttt ccaattttta 120 aagatacaac acacaaggac agggctagaa aaggacgaag tacaatgtct agctatactg 180 tgacaatgtt tcataataca gtgtgctcct tacgtagg 218 637 176 DNA Murine 637 ggtttttcga gacagggttt ctcgtatagt cctggctgtc ctgctgaaac tcactttata 60 gaccagggtg gcctcgaact aaaatccgcc tgcctctgcc acccgagtgc tgcgattaaa 120 gtcgtgcgcc accacgacct ggtctcttgt ctttctctta atcagctttc ctataa 176 638 182 DNA Murine misc_feature (1)...(182) n = A,T,C or G 638 gtgaatngga gggtggaggg agcaggagat gccctccccc agctcctggt atggacacct 60 cacttcttgc ataattcttg gcatcttctg cctgcaatgc tggctcccag cgtctcggag 120 ggggtcacac nctcatcgtc atgctcggag aataattgtc gttctggaag gagtggagga 180 ag 182 639 213 DNA Murine 639 agagtgtgac agtctcattt gtgcagatga ggaagctgag gctcttcaga gtcctatagg 60 ctggttctcc cagaggaagc ccacacagag agtgtagggg tttgagctct aggtggtcaa 120 cacctgcagg ggaccaaaac tacccagcag gactttcctc tctgagaggg gagagagcag 180 tccagccact gtcgtttgcc ctctgagaca ggg 213 640 154 DNA Murine 640 gcaataagca accgtttatt tataaaacca aagaagagag aaaaaaaacc aaacttaatt 60 attttacgac atcttataga ttaagtaaaa ataccagtat caatatatat gtatatacat 120 agtgcatata tatatgtcag atgatatggt aaca 154 641 213 DNA Murine 641 ataaaatggt atttaaaaaa ataacgtgtg aagaattatg catgaaacac tagggcattt 60 ttctgaagga gaaaagaaag cagtcattct cacaccagca gaggttcaac acaaggttct 120 ggctggaaat tccaccggct cctatttctc cctgtttttc ttgcaccaga tggcactgtt 180 tagttctctt ttgcagcctt gttctggaac ttt 213 642 212 DNA Murine 642 gttggcagca ctacgctgca agattctttt aatatgcttc tcaaaggagc tcggagtttc 60 agccagaact gcgcagtgga gctctgcaac ttcagcatct tcttgggtac agagcagggg 120 aggagatgct gggtggactg cacttaataa aggccgtatg agacattcat aggtactggt 180 gatgcagatc attgtgggcc caggatgtct gg 212 643 199 DNA Murine 643 ctgtctttaa tcaatcagga aagtatcaca caatcactgt gtcttttcaa tacactgtgt 60 gctccagaaa gactctcttg gcatccactt cttttctctc tgttgccagt tatagcctcc 120 tccagccagg tctcacctcc agccaggtct ctcccccgcc cccccgcatc ggtctaggtt 180 gtgtgtctcc ggggcgggg 199 644 209 DNA Murine 644 aaatatttca aaatactgat aaataatagt gactgcaagc agaattccag taccagagcc 60 aatggcccca aggaagtctg ctagtactga cagggaccga tgcacagacc cccaaaggca 120 gctcgtgtgg ggatgtacct gttcagctca tggaccatgg aggtatctct gtgacccctc 180 atcaccatct cgtgttcttt aagttcgtt 209 645 206 DNA Murine misc_feature (1)...(206) n = A,T,C or G 645 gtttcgacnc gctgggngtt tgtgctttca tcacatttgt taacaggtca aaatgcagan 60 cttcgtgaag accctgaccg gcaagaccat caccctagag gtggtgccca gtgacaccat 120 cgagaacgtg aaggccaaga tccaggataa agagggcatc cccctgacca gcagaggctg 180 atcttttncg gcaagcagct ggaaga 206 646 158 DNA Murine misc_feature (1)...(158) n = A,T,C or G 646 ctcgagcggc cgctcnnccg ggagtcagaa gattgaggtc aatcttcata atgagtggat 60 acctgtcatc aggctcactg agtggggaaa ggagctcctt ctcttccata ggagagaaca 120 ttcgttgccg aaaaagctat cttcttctca ctgagagg 158 647 228 DNA Murine misc_feature (1)...(228) n = A,T,C or G 647 gctaacnccc gagctgctcg gatgagccct ccagcactct ccaacattga tcttagcaga 60 gattacaatg ggctccatgg ctgctctgcc ctcagggctg atctgggcag ggacgctggc 120 gaactcaggg aaggaggcaa aggcactcag gttatccaca gcttccagca gagggctgtg 180 gctgctcggc actgggcacg gttctcttct gtgaagtccc atctagtg 228 648 229 DNA Murine misc_feature (1)...(229) n = A,T,C or G 648 gcggaannng aggggccaac taagaacacc aagaattctc acagaatgac tgaacttaag 60 agacctactt gtatgtcaat ctagatctca gagtaccacg cacaccatgg aatctccagc 120 tagcttctag gctgagtttt acaaagcagg agagagggtt tatacagtag ttgctaagtg 180 ttgcaaaggg cttttccccc aaaggttcgt gtgcctgaga gtgcgtatg 229 649 251 DNA Murine misc_feature (1)...(251) n = A,T,C or G 649 atcaaagacc cctaaatcaa gagaccatac catagattag aagactcaac ccaattaaaa 60 tgtcaattct tccttcaatc aatatataaa tgtaatgcag ctttctataa atatctcagc 120 aaagattttg gtggacatag agagcttatt ctaaaatagg gtggaagatg ngaggcagag 180 gcaggtggat ttctgagttc aaggccagct ggtctactgt gtgagttcag gacagcaggc 240 tacacagaga a 251 650 223 DNA Murine misc_feature (1)...(223) n = A,T,C or G 650 gaaagagtgc ggttcggtgg aggaagctag gaagaaggag ccatacggat gtggtggtga 60 agctgggaaa gggttccagg atggtggagc gagangttgg tgatgaagct agctggcggc 120 ttggcttgtc aactgcncgg aggaggcgag caggcattgt ggagaggata gatagcggct 180 cctagaccag catgccagtg tgcaagaaag gctgcaggga gag 223 651 190 DNA Murine 651 ctgaaatcac atatttgtaa tcatgaagaa acccaggaga gaatgccaaa ataaattgtt 60 tttctttgct gggatctaaa acatcaaggt tcgagagagg agggagtggg gaaaggagga 120 gtgggggaag ggaagagagg agagagagga ggagattaat ggctctgctt tctaaacacc 180 taggatataa 190 652 230 DNA Murine misc_feature (1)...(230) n = A,T,C or G 652 ggacgacgat gcgtagcggc ccggctgcgg ctgcggtgcc ggagcccgag gacattagcc 60 ctaaagacgg agagcacaca agagcaagct ctagacggcg tnnngcccag gtcggaaatg 120 agcccagcag tttcctcggt taggaaccca acccctgggt tagcagccac cttcacagat 180 tcgaacgagt ggttccagac tataaaccaa ttccacaggt aagataagta 230 653 259 DNA Murine misc_feature (1)...(259) n = A,T,C or G 653 ggcccagctg tttttcagga gcactgctca gggttggcca cctctgtttt tcccttccta 60 ttctaggacg tagagtcaga ctctgccaag cagttcttgc tggcagagtc agactctgcc 120 aagcagttct tgctggcagc agaggctatt gatgacatac cttttggaat cacgtccaac 180 agtggtgtgt tctccaagta ccagctggac aagatgggtg ctcttaagaa ggtgagtgcc 240 ctnnctgtgc cagggaggc 259 654 246 DNA Murine 654 cacaaaacgg tttttgcctt tccttgttga aatttcttac ctcttcctac actgctactt 60 tggcatgtac atatccagat caccagcctt ctatttcact gtgagccatt attaagtaaa 120 ttaaaggtta tttgaatgca agcatgaata gcactgtagt ccatctgaca accaaaccag 180 cagatcaacg tctgagtgat ccatgggtgg acccctacag cgtgaagcag ggatagctta 240 tacaag 246 655 233 DNA Murine 655 accgtacttc ctcctgatgg ctctcagatg gacccttggt tgtgtttcac catttcttta 60 catttactta tttgtgcgtg tgtatacagc cagggtgctc acatgggggg caaaggacag 120 cttgccgaag ttggttctct cctcctacca cgtgggtcca gggattgaac tcaggtcaat 180 caggcttgct gagaaggata ctccatagcc aggctagact caacttcgat ctt 233 656 235 DNA Murine 656 ccgtaaactt agtaacctac gtgactttgg aaagtctgca cccagataac atggtgcagc 60 ttctaagtag caaaaggggt gttgtgccaa ggttagcagg acacagtaac agcagcctca 120 ccaacagacc caaagacctg gtgagacagc tcagtgggca aagggacttg ccaccaatct 180 aataaccgag ttcttcagac tcagtagtgg aaggagagac ctgacgcctc attgt 235 657 235 DNA Murine 657 aatttttatg ttgagcatcc agaagtagca agactgactc cgtatgaggt tgatgagcta 60 cggcgtaaga aagagattac agtgagaggg ggagatgttt gtccaaaacc tgtctttgcc 120 ttccatcatg ctaactttcc acagtatgtg atggatgtgc tgatggatca gcactttaca 180 gaaccactcc ttcagtgcag gattcttgct cttagtggca ggatatgtgg cattg 235 658 199 DNA Murine 658 ctagaatcct gggattatgt caataacaag aattagcaca ttatacttca tagacattaa 60 tttcatcttt catgtacatt atgaatacag agaaacaact catctatttt caatggtcac 120 atgccatacc aattgtgtaa atgttctgaa aaatattatt tacagtgtta atatttcaca 180 atggaggcaa ggattgttg 199 659 184 DNA Murine 659 gccgtgtcct gtaaaacaag aaccacaaaa agataaagac aacctgaagg aaagtttcct 60 gccagcccca ggtcccccac agtgacactc gtaactggca gaagtttaaa cttgaatggt 120 gaattaaaat gaatcactta gtaggttgaa atttagagac ctagattttg taatcttaat 180 cttc 184 660 217 DNA Murine 660 cgagtcaggg tttctctgtg tagccttggc tgtcctggaa ctactctata gaccaggcct 60 cgaactcaga aatccacctg cctcttcctc ccaaatactg ggattaaagg cgtgtgcata 120 actacccagc ttaatatttt taataagaga acatcattgt ccactgcaat gagcttgata 180 atttcttgtg gcacagatga ctcagatctg aggagtt 217 661 212 DNA Murine 661 tctgtagctg ccaactagtg cctgtataga ctccacctgt ctggataccc catcaggcca 60 caccaaggca gctgtgaacg gggatcaacc acgctagcca gaaagcaaac gcaagggcgt 120 ggacaatgac aaccctgact gcctctgtgt caaagtctgc aagtagctga agtgattatc 180 acagttagca tggactgagg aggaggctca ag 212 662 235 DNA Murine 662 gccataatcc tatgaggtaa gtgtggtggt cagatgacaa cctcaggtgt cagtcttaat 60 cttttacctt gtttgggaca aggtttcttg tccattgctg ggcattccaa gctagctggc 120 ccaagagctt ccaggaatct cctgcctgcc tccctcctta ctgtaggacc tccggattac 180 aacatatcta ctgtctctgg cttacaggga tcaggctcag gcggcatgtt atgca 235 663 145 DNA Murine misc_feature (1)...(145) n = A,T,C or G 663 ccgcttcctg gcgctggcnt cagcttcttc ccgggaccgg cttcggcttc ctctttctgc 60 ttctgcagtc nctcctgctc ctcctgctca gcctgcgcct tctctcgagc ctcctgctcc 120 tcccgtctcc nccnccctcg gcctc 145 664 216 DNA Murine 664 gtctccttca tgacagggac gtcattttcc taatggcatg gtttcagaat gcaccaacaa 60 ggaatactta aaactgatga cctcataaca aggtttttta gtctttgcac tgaaatgtgt 120 gttgaaatca gttaccgtgc tcaggcggca aggcagcaca atcctgcggc taaccctacc 180 atgatccggg ccaagtgcta ccacaacctg gatgcc 216 665 209 DNA Murine 665 ccgaggccta gacctccaac tctggtgttc atttaacacc atgttgattt ttgtttctgg 60 caagatctta ctagttaacc taactggcct ggaactccct atgtagccca ggctagcctg 120 aaattgctat gtagcctagg ttagactcaa aaatcatggt ctccctcagc ctcctcattg 180 ctgggcttct aggtgttagg ccagctgag 209 666 177 DNA Murine 666 caacacacag cctccctgat cttgtgcagt ctttgtcacc gtcttctcta gtgccccaca 60 ctccacgagt ctgacaagca gcgtgtctgg gttagtaggc cttcttctga tgcaggctgt 120 gcagatgagc cgctctcgca gcagtgtcat ccagagggct ctcacctgca gccccca 177 667 247 DNA Murine 667 gtggaacctt cctggattta cggtgacttt gtccatatgt tcaaacagat agttgtagtc 60 taactcgaca ttcctgaggg cccggacagc atctcagtcc acattatttt cctcgtcctg 120 tgagatgatg ttttgctttc ctacctcagc ccaggagttc tatttccgaa gaaagaatcc 180 ggtggctctg atgatgaaga gacgatgacg atgacgatga atcatcagaa gactctgagg 240 atagagg 247 668 161 DNA Murine 668 agccgagcaa gatgcccaaa ggaagaaggc caaggggaag aaggtggccc cggccccgcc 60 gtcgtcaaga agcaggaggc caagaaggtg gtcaaccctc tgttcgagaa gaggcccaag 120 aacttcggca ttggacagga catccagccc aaaagagatt t 161 669 113 DNA Murine misc_feature (1)...(113) n = A,T,C or G 669 aagtctcaat tctgtttatt tagtggacgg tacactgatc atctcagaaa tctttcttca 60 tcagatgttt gagctccgtg caaagccttt nctctttgag cttgaacngg act 113 670 169 DNA Murine 670 gcgaatctaa atacaagtga ggagagggga aagaaaaaaa tgaaacaaaa tgaaatctgt 60 tcattttcct actgtcctgc ctcctttaca ctgttaggga taggatggaa cctccaggag 120 ggactcttgg ctcaagttcg tccctctcgg aaacacttca gtgtgaacc 169 671 222 DNA Murine 671 gggggtgatg ttgtacaagt ggatcttttg tagtgtaagg aaaatgcatt tcagatagtc 60 acacaggaag ctattttctt acccgggatg tctgtattgt taataagaat gtaatttcat 120 gatacaagta tttaatatct tttttaagtg agtaaaaata ttctagctat tggcaaatag 180 attacagtag acaattagag gatatgatga ggttgactct tc 222 672 164 DNA Murine 672 ggttttcagt gtgatttgtc tttgctgttc tggagcctta ttagtgggtt cttgaggctc 60 atccctctgc taaggtgtcg ctcttgatgc tctctgagct ctctctctaa gccgactaat 120 ccgaccttca tactgggact taagagcagt cattcgacat caag 164 673 233 DNA Murine misc_feature (1)...(233) n = A,T,C or G 673 ccgccatatt gtctgtgtga agctagggga gcgcggctat tnntgccgct tccaccgcag 60 tgtgaagaaa aagggtctga aacaaagtct taccaacgtc tcgttttgaa cacagtgact 120 cgtggatctt taaacatcaa gttcgctttg tctgtcaacc tgtctgacat gtcggacccg 180 tgaacgaggc cagagttaca cagatgttaa cacacacaga cccgagatac tgg 233 674 203 DNA Murine 674 tttttttttc actgagaata tgatttattc catcagacat gctgcaatta tagacatttc 60 cacagagtat agcagtgtta aaaagtttgc atttgaacag ctgacatgaa taaacaaaca 120 gtgggtaggt gaataacctg caaacgcgag ttctggattc acttttttga gctgagatga 180 cagattcagc aggaactctg tac 203 675 198 DNA Murine 675 gccttggata agcttggggt atttctgggt tgaactattc ccatgtccta gtttcccata 60 gtcaccatct ccccagctga agacttcacc ttctgtagta aaagctaaat gtgtgaccat 120 cagagccttt agatgatgaa actttcttaa tagaccggtg aggctcaaaa gttaactttt 180 taaggtagac tgattatt 198 676 202 DNA Murine 676 ggacattgta gggacttaag gatggagctt ctggctattg tctcgcagat ccttcattta 60 aatgcagtaa atacaagctg accttgagtg ctgagcgatt ccttgagcag ctaactaaac 120 tctcactgtg acaattctgc caacgtctct cctgttaacc tggggatttc ttatgcggta 180 aactcatttg tagacgggct tt 202 677 208 DNA Murine 677 tagaaatgca ggtcctgagg tgatctggat gacatctcag ggtgaaatga ggaacaagga 60 gcagaggtca ctggaaatgg gcatgatggt gagtgcctgg aatctgagta cacaagagtc 120 tgaagcagaa agggagcact tgaggctaaa acaggctcta tgttgagctt ggttggtttg 180 ttgtttgttg tttttaatta aattttcc 208 678 174 DNA Murine 678 gaaactgggt ttcactacac accagaggcc aactcgacct tgcagaaaat cctcctgctt 60 ccagcctcct aagtgcttgt ttacaggaat atgccattgt gcccagcaag caggtgtctc 120 aaaggtacaa ctggcagaca ggaaatctgc tgggcaggga ctctatgaac cgac 174 679 192 DNA Murine 679 gttttggttt caggaactac atgaatgaca gccttcgcac agatgtcttt gtccggttcc 60 agcctgaaag cattgcttgt gcctgtatct accttgctgc ccggacactg gagatccctt 120 tacccaatgc tcacattggt ttcttttgtt tggagcaact gaagaagaaa ttcaagaaat 180 tgcttaaaat ct 192 680 208 DNA Murine 680 gtgggtcagg gtatgagatg gttgaattgt ttttctcccc taaagcccac ccatccctca 60 tctagagttc ccatagttgt aaaggaggca cactacttcc actaagtact gaaattaccg 120 aagtttcagt tgtagcattt gtctctccac actttaactc tactaactaa tcgagtgagc 180 tgtcttccat ttggataaac ttgtaggg 208 681 214 DNA Murine 681 aaacaacatg gctcacgggt aaaggcgttt gttctgagtt cgatccctag tccccacatg 60 gaagaatgag aaaactgact tttatcgtcc tccagcttcc acattagcac caggtttgtg 120 cttacctggg catatatagt atgcatacat tctcacaaat aaataaacat caatctgacc 180 cacacttggg agcctgaggt gagagattgt tgta 214 682 169 DNA Murine 682 gcgggttgga gaagtggggg cctgtgctgg ctttgttgct aagctgtgac atcctcttcc 60 aattccggtc ttactcgact tcctcttgga agggcgatcc ctgagactca gccctttgga 120 acaaagaatt tgggaaactt agggctaagt cgcttttttc ctcctcttt 169 683 216 DNA Murine 683 cttggtttgc aggtcagatt tacactggtc aagttcgttc ttactatcca taaataactc 60 tgtagcctta ctgagctcct cctcaagaac agcgattttt tcaaccaact caacaatttg 120 ttcctcctga acagttacct ttccattcat ggctctaaaa ctttcttcag agatgtacac 180 tcattttctc acgagctgct gcttgatctg cttcaa 216 684 214 DNA Murine 684 cactgagtga ggacggaaga tcacccatct ccatccgaca gctggcctat gtttctggtc 60 tgtccttcgg tatcatcagt ggtgtcttct ctgttatcaa tattttggct gatgcacttg 120 ggccaggtgt ggttgggatc catggagact caccctatta cttcctgact tcagcctttc 180 tgacacagca gccattatcc tgctccacac cttt 214 685 243 DNA Murine 685 ctagattgtt cgctccaagt ctttatttta ataaggaatg actttagact cgcttttgtt 60 aattcggtag gaagtctaaa tctaactgat gaatttttta ggcacaggaa tcaagcccat 120 aatttgagaa acactggctt ttttattaac taccttcctc catttaaaaa taagtatgga 180 gtttttctgc ctccctctaa aaatactact tatttaaata attaaggaag agagaatgct 240 taa 243 686 228 DNA Murine 686 ccaaggactt gggttcaaat ccctgaacca gctgtgtctt gaacaatgac tcttgtagga 60 agacaatcta tttatttata ttttgtacag tgctcagtgt gtttcttcat ttaaaagagt 120 caaggactgg agatgtggct cagcaattaa gagcttgtta tgctcgcaag gaatggagtt 180 aaatttccag cacccacatc acaggctcac aactacttgt aacatcag 228 687 213 DNA Murine 687 tgtgtatgca cacacatgca tgtgtgttgg aagtgtgtgt gcgcaggtat gagtgtgtgt 60 atgtgcacac atgcatgtgt cgattggtgt atgtgtgtgt atgcaagtat gcgtgtgtgc 120 atgtgcacac atgcatgtgt gtgtgtgtac gtgtacatat gcacctgtgc acgggggggg 180 ggggcagggc tatggaagtc agaggataac ttt 213 688 178 DNA Murine 688 cactgacctg gacacttggg cttctgcgtg tgtgtggaga ccagaaccgt cgtcgcgttt 60 cggggccaca gcctgttgct ggactcctaa gactcctgcc tgactgctga gcgactggtc 120 ctcagcgatc cggcatgatg aagttcacgt ggtggcggcg gcgattgctg ctgctggg 178 689 200 DNA Murine 689 gggaactgta tgcaaaacgg ttctgatttc cagggacaga cccagcagcg ctgacaaagc 60 ccttcaccgt tcccacaggc agaggggagt ttaccatgag gtcggaatgt cagccttact 120 gactctcaca ctcattgtgc taccagctga ctcccagcca gtgacaccat ctttcatgat 180 tagcgcacct agcctggcag 200 690 177 DNA Murine misc_feature (1)...(177) n = A,T,C or G 690 ctctgtctct gcctgtctgt cccagtctct ccttccttcc ctccttcgng cctggcgttt 60 cccgtcgccc atgtagctgg aaaaagagtc tagcccgctc ttcccctcgc ctgtgcccgc 120 tcttacccga cacccaaacc gactgcctgt cccggactgt caagctccgg actgagt 177 691 227 DNA Murine 691 gggcgcatct ctttatctgg ccagatgtat ttgatgatta taatggcggt gacgtaccag 60 gtgcccacga cgctcaggaa gcagaaatgg ctctccggtt gcctttgatc agagtctctg 120 tctggcccag gctgggttgg gttgggacct ccgaacctgg catatttctg gaagccaatc 180 tccttggggg atggacaggg ggcaggatga agaggaaggc gtaagac 227 692 189 DNA Murine 692 ataagtgcga gctgagtctt cttttcactg atgtggacat tcagttctcc ttttctgaaa 60 acttagttct tccagaagtg attttacttc cctaatgtga ctgcacttca cagcaacttc 120 cgtctccccc agtgtcaggg cttcctggag gtcagctttt gcctgggcga ggatctgtct 180 ggagtagag 189 693 184 DNA Murine 693 ctgccaacca aagcagctta ccaagaagca ggtcacctgt taaggggaat ggagtaccca 60 agcacttcac ttcctcaaaa ctgagtgtgg gccagtctgt aaatggagtg agagagtaca 120 ggagaggggg gcctgaacct taggttcctg tgtaactgag aaccctgctc tgtagagccc 180 ataa 184 694 143 DNA Murine 694 atggcagcga cgtgagcagc gttgtggaag acaaaagaac gacagtgagt tgatgacttt 60 cctttacatc gaaatgcctg tgaaggagat tcagaattac tgagccatct tctcgataaa 120 gactttagtc aaccaactag atg 143 695 239 DNA Murine 695 agtttcataa agggtatagt aatgttcttt tataagaaaa tgtagcccat ttcttcccat 60 ttcattggct acaccttgac ctaacgtttt tatgtttgat tcttttgctt actttaatac 120 ctttttaggg ttttgctgaa gatggcggta tataggctga attagcaaga gatggtgagg 180 tagacgcggg gtttatcgat tatagaacag gctcctctag atggaatata agtaccgcc 239 696 148 DNA Murine 696 ctcgacggcc gctatttttt tatagcatgg ttttagtgtc catttggtat cagtgaatgg 60 atattgcata actaacaaat attctttcta tgtcaagata tttcattgat ttcttggttt 120 gttgcaaacc atttcattct taacattt 148 697 217 DNA Murine 697 tgtgaacatt ccatgtcgtc atgaaccacg cccttccccg tcacccaaag gtctcaatgg 60 catacacctc tccttcctcc attcttgtag cttcccctcc tttcacaatg ggcaccgttt 120 ttccagcgtg aattctatat ggcccaattg aatgtccatt taagttacta tgggtttcac 180 ttggtatgtc ttcagatcta tttctacttc ataggat 217 698 199 DNA Murine 698 gcaaaaagat ctactgatta ctttgtagta ttcacacaca aaaataaata tttacacaaa 60 ttcaaacatc caggagtcct actgagcagg tggggaactg ggcacatgtt tggccagctg 120 tggggaggcc tgggcactgg agtttgtgct gtatgaaggg gctcaggtgt cctggccagt 180 cagaggcctg gtgagcggg 199 699 182 DNA Murine 699 gtatggaggt gatgaatatg tattatcttt actcagtagg ctttttctag ggggcataaa 60 ctttgttcca cagatagctt tgtatggtac aaagttttgc acttatcaga ttgttgggtg 120 ttgggattca aaccaaggct ttgttgtata cttcaatcgc ctttcttatt tatttgagcc 180 tt 182 700 209 DNA Murine 700 catcctagaa tctaaacgga aacatccatc ccaacaacag ctttataaag ccagactcaa 60 gctttgggat gctgggctga ggtaaagacc gtagccccaa tactcagctt gttgggtaca 120 ctgatgtgct gttgagaaag ttacctccta ctgtggagaa tacaaaactc cgatggaggg 180 cccttaggaa gtgtagtgtg tatgaatgg 209 701 170 DNA Murine 701 ggagatacta gatgtaagca aaacagtttt ttgaggtttt tcttttctct ttttcctatt 60 tggtaaaatt aatactatcc ttgaaaacta ggctatttca catggtaaag caagagggta 120 caaaacacag gcaggcaggc aggccacttg gtatgtatct ttaccccttt 170 702 167 DNA Murine 702 attctttaaa agattattct gctcgcaaag tttaaaaagt gttgaaaaat attctataca 60 tcttgctctt agaacgtctc cactttgaca gatcaggtga ccctccacat catcctctat 120 actcctggat cttttccttc ggtggctctt ctggaacggc aagaggg 167 703 268 DNA Murine 703 ctttaaaagc cagaactcac atttgggtct ttgggacaaa gtcaaaacaa gagaaacgag 60 acacacatga acagttcctt catttcttag tagctgtttt tcagtaagcc agcagttttc 120 tagaaatgac tgtttttgta acccttcccc tacttggagc atttgtgcaa tataaacaaa 180 catttttgtt gttgttgtta ctattccaac agacaggtac tactatcaac tgcatctgtg 240 gatagaggca aaggatgagt caacatct 268 704 231 DNA Murine 704 ccgccgtctg tgccgccgcc atgtctctag tgatccctga gaagttccag cacattttgc 60 gagtactcaa caccaacatc gatgggcggc ggaaaatagc cttcgccatc actgccatta 120 agggcgtggg gcggagatat gctcatgtgg tgttgaggaa agcagacatc gacctcacca 180 agagggctgg agaactacgg aggacgaggt ggacagtgat caccatcatg c 231 705 230 DNA Murine misc_feature (1)...(230) n = A,T,C or G 705 gtcgtcctga ccacaaagct acatgaaggc ttcacaaact ggccaagccc tgtgtcttgg 60 aactggaact cgaaggacgt nnggccccac cgtgatttgg tcggtgagtt gggagcagct 120 gtgcgaagag gaacatacgc tacgcctcta ccactcgctc ttggaatcct tccatccact 180 ctacctactt gataagaaaa tggcttcaaa ctagatttga gggcaaaaca 230 706 203 DNA Murine misc_feature (1)...(203) n = A,T,C or G 706 cgtgaagcta ggaagagtgg gtctaagcca gagaactttg ctttgattag tgtgtcacct 60 cacccgagtg ggagcctgag cagcagtggc gacggtgtgt ccacagcagt gaaaacggng 120 gagcaggcta gcaagccttt accaccgcct gctgccgccg ccgccgatcc gcttacagtg 180 cctcttctga agaaggcagc agt 203 707 250 DNA Murine 707 gcactacaag gtgacattcc cagaatgtgt taggtggatg acaatcgaat ttgaccctca 60 gtgtggtact gcacagtcag aagacgtcat ccgtttgctt attcctgtca gaaccattca 120 gaactctgga tatggagcaa aactgacatc tgttcatgaa aaccttaatt catgggtaga 180 attaaagaaa tattcaggat cctcttggat ggcctactat ggtttagtgt tgccaggaat 240 gagcctttct 250 708 220 DNA Murine 708 aattactact ttaaatgggt gaatcatgtg gcatacaagg tatgtgccaa tacagttgct 60 tcttaaaaat tacatgcata tgtatgtaca tatatacatg cgtatgtatg tatgtatatg 120 catgcatgct attccctaag tagaaagcct acttcagtaa agccagtgta gcaaaggcaa 180 aggcacgtgt agagagatgt tacacgagta ctcagacagt 220 709 216 DNA Murine misc_feature (1)...(216) n = A,T,C or G 709 gggggctaaa gtctgtctac attacagatg gggctggact gtactctggt gttctggggg 60 atgtgggata gatgagaagg ggaaggcagc ttggcctgtc ccacctattc aagtgcctcc 120 ctaaagcagg atnagaaagc taggtcccta gccaataagc agagcctttg gctgagggag 180 aaagtagtca cccatgagaa atccaaccac aagttt 216 710 264 DNA Murine 710 ctcccatgca agaggtagct tgcccatgcc agtggacaag ttttccaagt tattttcttc 60 attatagatt atttctgatg tactagttga aattccaata tatcttgtag tatttccagt 120 agaaagagag gtgttatcag ggaggcaagc agaggcgtta ggctaggagt tttgtagact 180 gatgttgcct ggttgaggaa ggcataactg agcagatcgc ataatttctc tcgagtgtga 240 gtcactgcac attgatgata tgcc 264 711 271 DNA Murine 711 atctgatgcg gctgtggaag gaacagcgga ttcggcactc agtgtaaagc cacctgatgt 60 ctgcatcagt ctgtcaaaat tgttttcatc aggcacttct ggattagcaa aagacagtgc 120 aggcttgtag tttgaaatat ggatgcagaa gacggaagga ttgatctgat caatgagttt 180 aaagtcacgg tgaggtgggt ctgtgtcaaa ttcattgagt agaaggcagc cttctgacta 240 caaacagctg tcacccgtgc aactgactga g 271 712 222 DNA Murine 712 ccacagttta cattaccggc cgccatctgg atacgcttag agctactgcc caggaggcac 60 agtccctcgg gggccggtgt gtacccgtgg tgtgtgattc aagccaggag agtgaagtga 120 aaagcctgtt cgagcaagta gatcgggaac agaaagggcg gctagatgtt ctggtcaata 180 atgcctatgc tggcgtcagg caatcctgaa caccaccaac ag 222 713 185 DNA Murine misc_feature (1)...(185) n = A,T,C or G 713 gcaatagctt agattttatc aacattagac ataatagatg gaactgtaag acaacacaga 60 agcagtaaat atccaaattt tgtacttaag ctacagcatt gctatgcact tctctaactg 120 atttataagt gttgacacag tgttaangca cacacaggag cataaaagga agaaggcttc 180 tcctt 185 714 277 DNA Murine 714 gtgcagctag ctctatttta actgaagagt gggtggcatg ggatgtaaac agctgtatgc 60 acatgactag tctacaagtt atgagtcaca atcagacgaa acgtgactta gacttctcac 120 acatcactgc cccagtggtg taagagcagg cacccaaaca ctgaccgctc catttcaggc 180 accagcagtg tatctggcac aaatgataga gtgatttggc ttagtactat acttgtacca 240 agtagctatg gtaccagtgc agtagactta gctgaca 277 715 144 DNA Murine misc_feature (1)...(144) n = A,T,C or G 715 tctttatcac tgataagttg gtggacatat tatgtttatc agtgataaag tgtcaagcat 60 gacaaagttg cagccgaata gcagctgacc cgtgccagcc ctggacctgt tgaacgaggn 120 nggcggtaga cgagactgac gaca 144 716 188 DNA Murine 716 aaagctgcca tttcagaacg tgaagaggtt agagggagaa gtgaactgtt tcatcctgtt 60 agtgtagact gtaagctaag gcaaaaagca accacaagag ctgacaccga tgtagacaag 120 gcccagagtt ctgacctgat gcttgatact tcatcattag atcctgactg ttcctcaata 180 gacattaa 188 717 174 DNA Murine 717 cgccctccgc ccctgcgccc cgccacccag gccgccgccc cccgcccgcg ccgtcgcccc 60 cgacggagga ccatggcgaa ggtggaacag gtctgagcct cgagccacaa cacgagctca 120 agttccgagg tccttcactg atgttgtcac caccaaccta aagcttggca accc 174 718 182 DNA Murine 718 ttgcatctgt agatttgttt aggttgagcc tgtattttta ttgtggagta ttatctcatg 60 gagtgaagag tttataaata ctcactgaaa gcacacttac tcccaagctt taactcctga 120 ataaagctct ggtgaacatc tgtatacagg cattagtact aaatgtcaga acacttctca 180 tt 182 719 165 DNA Murine 719 cagaaaaaaa atagccttcc cgttttgtca gagattaccc gtccactatt acccggcagc 60 agtctagaga ggatggagtg tatcatgagc agtacagaat cttaatgcaa ttcatgagga 120 ccccttagtc cttccatgaa tctggctgct aacattgcta ttttt 165 720 170 DNA Murine 720 acatggtgct gaggagagga gccaccatca ccaccaccat gaggctccag actcttccca 60 tgggaaaaag actagagaga gtgaacgcaa tcatcggacc actgaggcag agccaagact 120 cttgaagagc caaaacacga gaccaaaaag ctaaagactc tgtcagaata 170 721 162 DNA Murine 721 aatttaaaaa tcttttataa ggacagtgac tgtttttacc aaaaaaagga aaggagccaa 60 agggcatcca acagatagtg agggagaagg gggccaggaa tccattcagg aaagttggta 120 actgctgcca cggaactgca ggggggaggt ggaagggaaa gg 162 722 189 DNA Murine 722 gaccttgggg cctggatggg gtgggcaagg tagtgaaggt tactagagaa aggtagggtg 60 aagaaagaag ggtctgagtc tgggttggag gggagtgagg acttggggtt cagaggaacc 120 tcaggagtgg ctagaacaca gctctaccct gggctatgct gtaacagaaa ctgtctattt 180 cagccacct 189 723 226 DNA Murine misc_feature (1)...(226) n = A,T,C or G 723 ugssngthuy tynchckcct gcagaggttt gccggcagaa gcagttttgt acaggaagcc 60 gctgtggctc acagttggca gggtaacgga gtagtgcttt tccaagggct tcactgagtc 120 caaccgaggg acctctgtac tctgattctt ctgctttttc cttcccctgt cccccaagtg 180 ttgagcttat gggccaagtt tatgaaatgc tggaaattca ctcttt 226 724 188 DNA Murine 724 tagggggaac gtagttattc agtggtatct tacatatggc ctagactatg cttaggctca 60 aaattgctta atcttaggcc cagggctagg gcggacgtaa tgaggagggt ggcaaggcag 120 caataaggtt cctgcctcct tctccctaga gagcactttc atggcccact gactactcca 180 tatgctgg 188 725 113 DNA Murine misc_feature (1)...(113) n = A,T,C or G 725 gcggtggcgg gcggcgctgg gacccgggag cggccggaga acaagggagc tggcggcccg 60 gcngcggctg agtggggctg agcagcgggc cncccgagcc gagctctccg aga 113 726 182 DNA Murine 726 ctggtctaca gagtgagttc taggacagcg aggactatag agagagactc tgtctcaaac 60 aaacaacaag caaaatattg acttgttact gaattgggaa gataagttcg tgagttcatt 120 agacttttta agatttattt tacttatgtg tacgaatgtc tgtgtacata agtgcagtgc 180 ct 182 727 193 DNA Murine 727 ccgaggttca cagtgaggtt agtgtccagg aggaaactgt cttcacatga actggttagc 60 aggacttagt gcatcgagga ctatggaacc ttgctcctga atccagcaac tgattagagg 120 aggggataaa agggaagcgc ttctgactca gtgtggcagc aaatgcctgg tatccatcac 180 ccaagggtag ggg 193 728 167 DNA Murine misc_feature (1)...(167) n = A,T,C or G 728 gcgctccgca cccagctcct cccgcgcccc gattccacga gccccttggc tnccaatccc 60 gggcaagcac cagcaaggca cgccggggcc gagagtccgg cncccacgga gcagagccag 120 gccggccagn cgccggtgtg ccacgggcca acgcttcttc aggggcc 167 729 173 DNA Murine 729 aggaaaggct gtataaatgg aaagaatatg ggacaccctt tatctatggc tcatccctta 60 ctccatatca gaaatttctg aaaggagaca agcctgagaa ctttaattca gtcgctgtga 120 tccaagacag caaccagaag agtagtgagt agaatgcaaa tcaaaataga gtc 173 730 211 DNA Murine 730 cagacgacga cgatattcca gagatcccag acctggacga ggagagacgt cgcagctcgg 60 ccacctccgg ggccaagatg ggacgccggg ctcatcagga gtcgactcag gctgagaatt 120 acttaaacag caagaattct tcactgactc agactggaga ggctccccca ccgaaaccac 180 ctcgaaggca gggaggctgg gcggatgact c 211 731 209 DNA Murine misc_feature (1)...(209) n = A,T,C or G 731 acacgggcac gagcgatgat ctgtgtgcac tcgtcaatga ggtctggatg cgaanccgac 60 gctgcagtga gtacctgcag cgcttcgccc ctgtgccacc accaacgagc cgctgtaaag 120 gaactcgacc agctgccgca tgtctgcnag gcaccactgg tggcacacga atctctgagt 180 ggccgagcag cagtttatct gaaagaagg 209 732 225 DNA Murine misc_feature (1)...(225) n = A,T,C or G 732 tccaagnncc ctgcagttct cttcgaaaga agacgaggag tgctctgcgc ctgccgtaag 60 aacagccctt tttgacttaa ggtggcggtg gtactgtgac acaggaagaa ctgcatgcag 120 gctgcaggag tttcttattc tcacttcttg agtagttagt tagttctccg tggagggaga 180 aagatttgag tcttaattgc cctgttttca aggtgttctg tgtcg 225 733 199 DNA Murine 733 cagaatgaga ccttggcttg gaactcagtg atctgcctgt ctctaactcc ccagtgctgt 60 gattaaaggc atgccaccta gaactcacca tatacctgag atgggctatt aaatgatagc 120 ccacccacta ccaccgcatt ctgtttacgt cactctattt ttcttaaatc atttctaata 180 acaatggagg aagagaatt 199 734 208 DNA Murine 734 gccgtagcca tcatgaatga cacagtaacc atccggacca ggaagttcat gaccaaccgt 60 ctgcttcaga ggaaacagat ggtcattgat gtccttcatc ctgggaaggc aacagtacca 120 aagacagaaa ttcgggaaaa gctggccaaa atgtacaaaa ccacaccaga tgtcatcttt 180 gtatttggat tcagaaccca cttcggtg 208 735 174 DNA Murine 735 cctttccagt tttattttaa agttttaaaa ttacaaccac agtaatttct cccgggcact 60 agttacactt gtttaaaaag gggtggggta gggtgcgcga gtttctaatt ttattaaaat 120 aagagaaaga caactgtaca gattttttct cccagctccc gaagtgagac taag 174 736 165 DNA Murine misc_feature (1)...(165) n = A,T,C or G 736 cccggctcga gcggccnaag acaaagtttc tgggtagccg tgtcctggaa ctcgctctgt 60 agaccaggct ggcttctatc tcatagagat ccgcctgcct ctgcctccct aagtgctggg 120 attaaaggtg agtgccacca ccacccagtc aatttagcaa ttttt 165 737 175 DNA Murine 737 gtcaaataat tcagcagaat gaaatagtta catacatccc caaatttata cctttaaatc 60 cacagcttca gttcacacta aggcttatgt tagctatggg ctctgcatgg taatgaggca 120 tcagtattgg ttaacaaaaa agatgtagca ctctataaaa gaatgaaggt tgagg 175 738 180 DNA Murine 738 ctgtcttgta actccagaga acttttatag gatttgatta aaggttttaa taaactgttt 60 aatacttaaa gttagaagag gttgactgta cacatggtct tgtttggatg tgttggctcg 120 tgcctctaat ctcaacactt tgggagggct gagacagaat tgccacaagt tcaaggcaaa 180 739 190 DNA Murine 739 cagccagctc ccaggagccc gatgagcttg aggagctccg aggcaagaat gaaagcctca 60 ctgtgcggct gcatgagact ttgaagcagt gtcagaacct gaagaccgag aagagccaga 120 tggatcgaaa gattagccag ctttctgagg agaatgggga cctttccttt aaggttcgag 180 agtttgcaaa 190 740 189 DNA Murine misc_feature (1)...(189) n = A,T,C or G 740 cccggctcga gcggccgcng cctgtttact tctttgtttt tcctaaggat ggctttaaaa 60 taccatcagt gttccttttt ggaaggagtt gcttcccggt tgttatgtct gtgggatatt 120 tttatccaag aatcaaattg ttactgaatc tctctgaaac tccaggcata gtagcacact 180 cttatataa 189 741 183 DNA Murine misc_feature (1)...(183) n = A,T,C or G 741 cagcagaatn tatgccacta tatacaaaca caggacaacc tgaagctaaa tggatgccca 60 ccgcagtatc aacaggtcca gcctcacagt gcacgccctg agctacggcc ccctccaaaa 120 ggcatcttcc cctcacagcc tccacgccaa acaaggagca tcaagaatct gtctgggttg 180 ttt 183 742 178 DNA Murine 742 gtctgtgtga ccaaggactt cctgtcactt ttcccttttt aaaattgaag gagaaacagc 60 ccagtagctc caccctttct ggccacagat ggcattccgt tagtttgcaa tagccacctt 120 atggttaaag ccaacaggtc atacagcttg gtcccagctc gtctgcctgg acgctgag 178 743 182 DNA Murine misc_feature (1)...(182) n = A,T,C or G 743 cgccggccga ggagcagccc cagcaacagg cctcccgacg gcccagggca tcagccgcgn 60 cgcgaanccc cgtctcctgc tcagggcaag aagagtccgc gactccagtg tatagaaaaa 120 ctaacaactg ataaagatcc caaggaagag aaagaagatg attctgtctc ctcaggaagt 180 tt 182 744 499 DNA Murine misc_feature (1)...(499) n = A,T,C or G 744 ggatctatgc attatgtgtt aggagatttt tgagycatct atgtcanctg atcctaggga 60 tccattcatg tggtggggct ccatgccgsc catgcctccc attgggccgt ctgagccagg 120 acccattgga aagttagacc ggctgcctcc aggcggcaca ggattaatca ttgtgtagat 180 gttgtcactg gaattybttg aatctgcagg actaggcatg atgggggttc ctggagggcc 240 accaccacca ggggggtccc acataggttc cgggtgatga tgaggagtac gggattgagt 300 tagcactgtt gggattgggc cagggtctgc cagctcctgg gsccatgtta atcccaggva 360 tggcggggcc gagggagttk ggtggtggtc tmatgccgct gccgtaattc tgtgggncag 420 gcccatgggc ccatgcctcg gggagggttm attctctgma ttganncctc caatgttggg 480 gtraccttgt tgtcgtgtg 499 745 594 DNA Murine misc_feature (1)...(594) n = A,T,C or G 745 ngaantccga gcgggcgagc gccgggnngg gggccgggwg cwrggcarct cgggwgrncc 60 ggacggtagc ggcggcggcg gcggcgggct cggcgccctc ttctctgcaa gccatgtttg 120 ccaaaggcaa aggctcggcg gtgccctcgg acgggcargc tcgggaaaag ttagctttat 180 acgtctacga atantttact gcacgtwgga gcanmagaaa tctgcacaga ccttcttatc 240 agagattcga tgggaaaamh mncatcacac tgggtgaacc ncctgggttc ctgcactcgt 300 ggtggtgtgt attttgggac ctttactgtg cagctcctga aaggggagac acttgtgaac 360 attcaagtga agcaaaagcc tttcatgatn nthtgtgcag cagctgnccc caagccctgt 420 gcttggcaac attnccccca atgatgggat gcccggnvgs cccatcccgc caggkttctt 480 tcagcctttt atgtcaccgc gatatgcagg cggcccaggc cccgnatcag aatgggaaac 540 cagcctccag ggggagttcc tgggacacag ccacttgctg cccaattcca tggg 594 746 102 DNA Murine 746 gccatstggt ggattcgccg ggtwmgtcgc ctgggctggc aattgtgttc tcttcgtgtc 60 tgtcwcacch cystcccggc tcttctggct ttccacgtct ct 102 747 349 DNA Murine 747 ggaattcaac agaatacaag aaatggaaga gagaatctca ggtgcagaag attccataga 60 gaacatcgac acaacagtca aagaaaatwc aaaatgcaaa aggatcctaa ctcaaaacat 120 ccaggtaatc caggacacaa tgagaagacc aaacctacgg ataataggaa ttvatgagaa 180 tgaagatttt caacttaaag ggccagctaa tatcttcamc aaaataatag amgavmmctt 240 cccaaacata aaavvmgaga tgcccatgat catacaagam gcctacagaa ctccaaatag 300 actggaccag aaaagraatt cctcccaaca cataataatc agracaaca 349 748 165 DNA Murine 748 ggaattcgcc gggtatagta gcctgggctg gcatttgtgt tctcttagtg tctgtataac 60 aycystccag gctcttctgg ctttcatagt ctctsgtgam ahgtctggtg taattctbat 120 aggccttcct ttathyvykm ctygaccttt ctccttacyg ctctt 165 749 94 DNA Murine 749 tctagatgca tgctygvgcg gccgccagtg tggtggaatt cccggtctcg agcggccgct 60 tttttttttt tttttttttt tttttttttt tttt 94 750 531 DNA Murine misc_feature (1)...(531) n = A,T,C or G 750 ggaattcgtg atcatgaagc ctagtdcgct cattacacaa bbbsggggga ggdctcagga 60 cctctccacc ccgggagtca tttccctgtg tgctgtggaa ctaatttgaa aagtaaagtc 120 caaggaaaca ctgctctgtt tctgagacat gaaggaaatg aaaacacaag acaaagcaaa 180 gagctgcgca ttctctggcc cacgcaccgc gagagagtgc ccaaggtcag ccatcaccca 240 ctagtgtaaa ctgaccccaa gttagctccc cagcgagcag gctccaaaca cccccatcct 300 tcctttctac ggaggtttgt gaataaaatc accctcttcc ctcagtacca catctcttaa 360 ctttatctrc ccagcnatgg ggtgagcagc cagactynga tctkgtaama cttacataac 420 ataatttgag tgctgagaat acagctcaga ggtagagcac acttgcctag tgctcatgag 480 ccacaggccc attcccagta tcatatacac aaasgataat tttstwgact a 531 751 498 DNA Murine misc_feature (1)...(498) n = A,T,C or G 751 ggaattccgc ttgacctgcc ttggggtatg ggtactgctt tgctttgggg tacagtgctc 60 cagtaaaccg aggtatgatc atgttaggca ccaacgagtc atttatcatc aggaaggcaa 120 gtctctctcc atcgggggac caccagtggg cgatatgaga atgcagaagt tcttctagaa 180 taaatgagtg ttatnnyaca tcaacttcat ataaccagtc agcaatccca ttaaaaataa 240 tgccttcctt tcctgaagat gttagtcgta aagaactgct cttgatatca ggttgatagt 300 agatattgtt ttcaaaaata taaatcagct gctgtccttg cacaccccag gccgcatact 360 gcaacactga gtcctcaact tctgggggat tcaactccca canhttcccc agatnnaaag 420 tatgttcdcc tggcatacga cghycaagcr ghkdcatact cattaccgct tcatattgat 480 tacaacatac acactgga 498 752 1133 DNA Murine misc_feature (1)...(1133) n = A,T,C or G 752 ggaattcctc tctctctctc tctctctctt tttctctctc gctctctgcc tttctctgtc 60 tctactccct caactctctt ccccatgccc tgaataacct ctattctata ctacakgrct 120 ggkccctcag ggggaagggg tgcctcagca tgggcccgca gaggtacccc cttccccaca 180 cctgatggca ccaaacatat tccttctctc cttctctccc tgctcatcgc ttgaggtagc 240 atggttctct ctgggaagct ctgggkgctg agtcagggct ctgcntctgg cccycccctg 300 aaactccatc agaatctaca tggccctggr ctgtggcaat tkkcttcttg gdcccyaaca 360 agacttwaag nkyctygaag ggcaaggttt cttcccacta aatccagcac agggcaagac 420 acatagtagg tgttccacaa gcacctaatg agtgctctgg gttgttggga tttbyybbyg 480 tttgtytgtt ttggttttgg gktttgtttg ttkgttwgtt tgtttagyns gttttgcaac 540 aakgtctcaa gtgacataac ccagggtggc ttcaacaatg tkaacaaagg gtcyycyksc 600 yysrmcykky markksykgg rnnmmmrgss smmcmwkscy kgksywwwwk grktkrrccc 660 agggcctcag gcatgcggta caagcactct accaacagrt ctgcatcccc aaacccccaa 720 cagacaactc ttggtgcccg gagtgtgcca ggcctccctg gtagtactgg gcagacagca 780 gtccttgctc ttggccttgc tcctgctgga agcctccctt gacttcdtca ggcktttacc 840 tgaktcctac tctatgtcac tcatcaaacc tctgcgccca agaggarcng ycctgggaaa 900 acacaggagg cggcgacaca acactgtgcc tttcctcgct ggccccaagg acactcagca 960 ggggttgctg gtacaccktc aaccktttgt ctcttgcddd kktktctctc btcttgtgcc 1020 tttgaggttt catggttgtg gtggtttggg ttttctgtgg tcggttggca gtaaggtcts 1080 mnncataccc agtgttgttg dtkckgcaaa tgcntatata taatggnaaa tcc 1133 753 312 DNA Murine misc_feature (1)...(312) n = A,T,C or G 753 ggnattcggc anngraggga gcagagagga ggcacagatg ggccgtggga gaagagagat 60 aggagggcag aggcgatcgc sgktgcaagc aggaaatgcc ttcgcctcct cctgcaggtt 120 tgcaaagagc tctgaaaagg tctggcggtc cctgaagtgg gttgggggtg ggtagggctg 180 catagtgacc cgcacaaaga gagggagaat sgmgcacctc gaatatcctt caactcaaag 240 aaaaggataa mggmgagcaa atgcatgtat ctcttcagat agattbtgab ctsggccaag 300 taacttatat ac 312 754 294 DNA Murine misc_feature (1)...(294) n = A,T,C or G 754 ggaattcctc cctttgtnct gncagntttt tnccccttga ncatwncatt cattcattnc 60 attcattcaw tncagtgnaa gagcyycgtg kycagknatt ccagactccg atgaaahtyg 120 aaaatcgany cnyyycnnkt cyaattannn kcyaaycaga gagaaacgsy tccagtttcg 180 tncagcatag accatacata tatvcvvbkk kgkgcagtgt ctacaagtaa aacagttgct 240 ggagtaggcc ggccaaggat tctactcgtt tctaaataat cagatgactt ttta 294 755 87 DNA Murine 755 ggaattcgac aaattaagam amgcaaacaa atcatagctg ckcctswcgt kttttgtgcg 60 aaattgcaca agakcctata cacctga 87 756 444 DNA Murine misc_feature (1)...(444) n = A,T,C or G 756 ggaantccat aagtactatn awddnvbbvh wwwttttaag ttgaggctct aattagacat 60 cagcctgatt yctttsagtt ccacacacac acacacacac acacacacac acacacacac 120 acacactgtc ttcagcagtg agaccttaca atcacttctt agaaaacamt tgataagtmg 180 ccttgccaat agccagtgtt attttgggat tccatgggat ttcatggagt caacattggt 240 cagcaactca attagatgta agccattcct gggactgava ggtttccttg gagaggaaag 300 antgtctagt tggagtactg tttcccttgt tgtttagtga ctccatttag atttaatcat 360 atatgtatat attttaagaa gtttcaactg tagtaggttt ccatatggac cccaaaannt 420 cttagtgcta actgtccctc cctg 444 757 109 DNA Murine misc_feature (1)...(109) n = A,T,C or G 757 ggaattcccg gnctcgagcg gccgcttttc cccccccccc ccdcdcdctw cwcwcwctct 60 ctctctctct ctctctctct ctcgcgtcgc gggcgtaggt tctttattt 109 758 461 DNA Murine misc_feature (1)...(461) n = A,T,C or G 758 ggaattncaa gaatgtacgs cagaggaacg ncacctgagt ggtggggcag gcgggggagg 60 ggaggtgccc agggtgcctg accccaggcc agcntctacc tccactccag tatcccatcc 120 tgtcccgatt tgaacctacc caacccaacc tatcccaacc caagtgaaga cagagcctta 180 ccttacagaa aacccacctg gragaagcaa rccacttnca gcccctgttt ctaatttamm 240 ctaaatgagg tttctatgca gacaatccat tccttagggg tttatkttyb nnnnnbbsct 300 tcccttctga agtgttgtca ctacagccct gtkgagtkgg ggaatkgkgc cttgtccttk 360 gtcaggaggg aaggccagtg catgctctga cttactgttg gagggggctg ggcctsctgg 420 aaccccccca aataaagacc waccccacca aaaaaaaaaa a 461 759 407 DNA Murine misc_feature (1)...(407) n = A,T,C or G 759 ggaattcatg gcgcatcccg cacccctggc gcccggcgcc gcggccgcgt acagcagcgc 60 cccgggggag gcgcccccgt ccgccgccgc cgccgccgcc gccgccgctg ctgccgccgc 120 cgccgcggct gccgcgtcgt cgtcgggagg gcccgggccg gcggggcccg cnggtgccga 180 ggccgccaag cagtgcagtc cctgctcggc ggcggcacag agctcgtcgg gggcccgcgg 240 cgctgcccta cggctacttc ggcagckgct actacccgtr cgcccgcnat gggcccgcac 300 cccaacgsca tcaagtcgtg cgcgcagnct gcttcggccg ccgccnsyct tcgccgacaa 360 gtacatggac amcngccggc cccgyggcsa ggagtttagc ttccgcg 407 760 104 DNA Murine 760 ctcactatmc ccmtycccct ctmgatcatc tcacccccca atctctaaat tcccctyags 60 asaccaaacc tcarcctctc tctatccaat tcttcagtta gtat 104 761 514 DNA Murine 761 tccttahgck tttcttcaag ctgccctctg ctccaccttt taatccatta actagtggtc 60 ttgagtttat tgatgacttt tttctctttg accctcctgt tctggaacca gattgtgacc 120 tgcctctcag agaggtttgt cgtggctgat atcctcctcc gtttgtcctt ggtaatgaat 180 ttkttcgtag cgtattcccg ttcgagttct ttcaactgca ccttagtgta aggcacgcgc 240 ttctttctcc ccctcctata ggagctggcg tctgaaggat gggagacgac gtcgggcaga 300 gtggacttcc agaggtgggg aggctgcgtc tgctctttgg ggcagtacat ttggccgttc 360 cagccgttgg gcagagccca ggggctgata gctttccatg ggaagcccca gaggctcgtk 420 gcgcgactcg ccaggacccc cgagccccgg aactactggc atatccaggt agccgggcac 480 gggctggtga tggtkggtaa ggcccggytg ctta 514 762 406 DNA Murine misc_feature (1)...(406) n = A,T,C or G 762 ggaattctgg agaagtggga ggtgtactgt acggggaggg accaggggaa gaagaggggg 60 gtggaaagta agawgggagg ammggcaggm gggggagaga gagatgttac tgctttcttt 120 tcagcacata tamnncnnab grctanrgaa acgcatattt aaaatccagt ttctatattc 180 acacctaatt cacttccaaa cctacttgta aaaatccatc ttcagcaaat gaatttgttg 240 ggaaaatggc caggcatcca tacacagaaa ggttctccat caccataaat taactcatgg 300 tatgctgaat taattgttga aaattactag aaaatatgtt cacaaacctg gcaaattcag 360 actatgtcac acacaaatac tcctttcttt ctccctcctc ctccct 406 763 406 DNA Murine misc_feature (1)...(406) n = A,T,C or G 763 ggaattcgta attttagaaa anygcttgcg tattcnthhh nncgtyngtt ngaacgtggg 60 ggntytttnc tcaatagccc acattgacct tgaactcata aactcaagca atcctcctga 120 tacccaagta ngctgagtct acatgtctgg gcctctgtgc tcggttgcct ctgattttca 180 tcatctgaaa ctaagacctg ccaaagtngg gggggggggn cagttttcct gagatttcga 240 gacttgcatg cscggtactg gattgatgtg agagcaccct ctggggccat ctctgttcta 300 atncccgggc tcggtcttct gcagcctttg gaaaggttat gtytcagaat aggtgttggt 360 ttacaccgat gtttttcaat gcttcatagg cattbmmcaa gatamc 406 764 524 DNA Murine misc_feature (1)...(524) n = A,T,C or G 764 ggaattcccn ggctcgagcg gccgcttttt tttttttttt ttttcagaaa gccagtttat 60 ttctwagnct ttntncatan mhyyyhbmnc sggtaccaat agttaccygc catactcgca 120 ccaagttgtc tgtatagcca gcaaacagag tctkgccatc agcagaccat gccaaagagg 180 tacactgggg tggctctgcc ttkctgctgg tgctgataac ktcttnckbc aaktcatcta 240 caatgrbctk gccctccaag tyccagayct tgatgctgvg nncmrtggca gcscagagcc 300 agtagcggtt ggggctgaag cacaaggcat tgrtgatgtc cccaccatyt aaagkktaga 360 ggtgcttgcc ttcattgaga tcccacagca tagcctggcc atccttgcct ccagaagcac 420 agagggatcc atctggagag acagtcactg tgttcaggta gccagtgtgg ccaatgtggt 480 tggtctttag ctgcagttag ccagattcca cgaattccac caca 524 765 77 DNA Murine 765 attggccttg akcatctgag cggcgcagtk tgtgaatcaa gctgagcggc gctttttttt 60 ttttttcagc taactta 77 766 403 DNA Murine 766 gaattcgctc tccttccctc ggaacaacat tagctacctg gtgctctcca tgatcagcat 60 ggggctcttc tccatcgctc ccctcattta tggcagcatg gagatgttcc ctcggcacag 120 caactctacc gccatggcaa ggcctatcgc ttcctgtttg gtttttctgc tgtctctgtc 180 atgtacctgg tgttggtact ggcagtccaa gttcatgcct ggcaactgta ctacagcaaa 240 aaactcttag actcttggtt caccagcaca caggagaaga aacbgaaatg aagcctgctt 300 gataaactgc tctcgagggg taaaacctag gbctcccatt gagcagcgtk aagggagchg 360 tccagactct ccatcgattg tvgcatctgt gatgttkgvc acc 403 767 440 DNA Murine 767 ggaattcgtg gatctggcta actgcagcta aagaccaacc acattggcca cactggctac 60 ctgaacacag tgactgtctc tccagatgga tccctctgtg cttctggagg caaggatggc 120 caggctatgc tgtgggatct caatgaaggc aagcacctct acactttaga tggtggggac 180 atcatcaatg ccttgtgctt cagccccaac cgctactggc tctgcgctgc cactggcccc 240 agcatcaaga kctgggactt ggagggcaag atcattgtag atgattggca agagttatca 300 gcaccagcag caaggcagag ccaccccagt gtacctcttt ggcatggtct gctgatggcc 360 agactctgtt tgctggctat acagacaact tggtgcgagt atggcaggta actattggta 420 cccgstaaar gktttatgac 440 768 701 DNA Murine misc_feature (1)...(701) n = A,T,C or G 768 attcggcggc gtttatttgg agcaaattca gctcccggag ctggacggtt gaatgcagga 60 ggagttccac caattgctcc aattccttcc attgttgcag cttggccaaa acgttcagtt 120 gttggtgggg tcaatccaag ggttccatct ggcatcatag tggcaggtcc tggaggagct 180 ggagtaccag gtggcacagg agcagggggc atcgcgcctc tattgtttat gcccatagca 240 cctcccatag ccatttggcc catccgtatc tcttgttctc tcgcatcagg gaaggttccc 300 ttgaatcctt cctgctgtcg gcgcatcatt tcctcttgct gtcgccgcat ctctkcctca 360 cggcgcctgc gtkcctcktc ctgcctgagt tctaactgcy ttcgsttctg aaccycttkg 420 ktatgcagct cctccattct ccgaagctct tcttgacgtc tyatcaaatc cygcctcatt 480 agcataaccy ggkgctcwtg acgkgyagcc tccatctcca tctccagcnt tctcnacgag 540 cctcctngat gtnccgancc actngakcct gctgttgckt ctccatctca atgagtgcct 600 tccagcgcat ggcatactca tactcaaagg agcvavvnhg tgcaaatctg ggtggctgtt 660 ctctctcctt gtggaattgc tnnnnnttga nntccaccac a 701 769 323 DNA Murine misc_feature (1)...(323) n = A,T,C or G 769 cgdtaggcga gcagcgcctn ncctgaagct gcgggcattc ccgatcagaa atgagcgcca 60 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag 120 tgcgtcgagc agcnnccmrc ttgttcctga agtgccagta aagcgccggc tgctgaaccc 180 ccaaccsddn vvmsaktttr cgtgtcgtca nnamcgtctn acrbcgacct ygttncaanc 240 aggwccnwgg gcggmamgga tcnactgtnr wtcggsykyr ackttktnnc atsctngavr 300 ctttatcact gataaacata sat 323 770 640 DNA Murine misc_feature (1)...(640) n = A,T,C or G 770 ggaattccgc tgcctcaagc tggcttaagt cctgctgaga ttcagcaact atggaaagaa 60 gtgactggag tccatagtat ggaagacaac ggcatcaagc atggagggct agacctcacg 120 actaacaatt cctcctcgac tacctcctcc accacgtcca aagcatcacc acccatcaca 180 catcattcca tagtgaacgg acagtcttca gttctgaatg caaggcggga cagctcatca 240 catgaggmga ctggggcctc gcacactctc tatggccatg gagtctgcaa gtgngccagg 300 ctgtgaaagc atatgtgaag attttgngac agtttttgaa gcaccttaac aatgagcatg 360 cattggatga ccgaagcact gcccaatgtc gagtgcaaat gcaggtggta cagcagttag 420 aaatacagct ttctaaggma cgcgaacgtc ttcaagcgat gatgaccsac ttgcacatgc 480 gmccctcaga gcccaaacca tctcccaaac ctctaaatct ggtgtctagt gtcaccatgt 540 cgaagaacat gctggagaca tccccacaga gcttacctca aacccctacc acaccaacgg 600 ccccagtcac cccgattacc canggaccct ctggaattcc 640 771 279 DNA Murine misc_feature (1)...(279) n = A,T,C or G 771 ggaattcaaa rcagtaactc catagagcat cagnmaamga vtccatgaac gtacatgttc 60 caaatddgtn ngcctcggga gtagtcttgg gaatgagagt ccttacggtc caacatgctg 120 gcaaacgtta gctaacattc aatgtcagan magmgtgaga camgcttgtg cgcttacmag 180 thaamcagta gagttcttgc agagacagat ttstgnaggh nstcagtcac gatgacaaag 240 tctcacatag hhctbgcaag acaccgggac gattgasra 279 772 559 DNA Murine misc_feature (1)...(559) n = A,T,C or G 772 ggaattcgta cagtcaccaa agtcacattt cagaggaaat cttaatagat cttctcacag 60 ccaaaaatgc aagaagcaca cattttatag ttttnngttt gtatctcaga gcctcagtcc 120 atacagaaca aagtcagccc aacaaaatca gttcaaggaa aacaaaagtt aatttgcttg 180 ggcttcctag ctaacacttg gctattttcc cactcaggtg gaggagtgtg taattctgcc 240 agtgcccggg agctgagcac ccaggbtaaa acacacaaaa vmacacaagn ttaggntcct 300 ggtgnctkag aaagttacag ttagagcgga ggctkctkac agcctggrng ttcctggrat 360 ratcamaacn tccagcangc acaaccttga cttacaattg rcagctctgc tctactctgg 420 ggtctgaaaa ccccagagag gcgcaaagct gactctaaga ggcaaggtct gtcttgctgt 480 tgttctattg ccacgaagag acaccatgac caaggcaact ttgaaagcat ttaatttggg 540 ggktcatgga tccaagggg 559 773 100 DNA Murine 773 ggaattcgtt ttbytttttt tttttttttt tttvggtata acacaactkt krrtaadcta 60 ttacaatwtw tcatgaacaa ggtaaatcac ttgagtaact 100 774 569 DNA Murine misc_feature (1)...(569) n = A,T,C or G 774 ggaattcgcg tcggacctng cggagcccag gatggtgttg ctcgagagcg agcagttcct 60 gacggagctg accaggctct tccagaagtg ccgctcgtcg ggcagcgtgt tcatcaccct 120 caagaaatat gacggtcgca ccaaacctat cccgaggaag agttctgtgg agggcctcga 180 gcctgcagaa aacaagtgtc tgttgagagc cacggatggg aaamggaaga hcagcaccgt 240 ggtgagctcc aaagaagtga acaagtttca gatggcctat tcaaatctac tgagagccaa 300 catggacggg ctgaagaaga gggacaagaa gaacaagagt aagahgwgca aacyagcaca 360 gtgnacaggy gntggctgct accaaccags tgncacaang tgncattttn cnnnctcntn 420 gtttgctrvt ttncagcamc tstgtatgta actgtttcca cggaagggtc ctttaagaga 480 gaaggactgg gatgggcatg ggctagttgt bgtaagacgc cakttttsat tgtgcygtgt 540 gggctggata ttcttagatt ccagccgta 569 775 153 DNA Murine 775 ggaattccat tggcaatttc tttttccaat tccataactt tattcattbc caaagagagc 60 tggttttcat caataggcaa actttgttcc tgacgaatca gtctggccac agaaatcata 120 aaatccacat atgctgtgca agcctcttta tat 153 776 248 DNA Murine 776 ggtggattcc ttccttaata gtgttattvt gtttgtttct atgvttgtcc ccctcccctt 60 ttcttgaggc agcgtctcac tctgtagccc agacttgacc tggaactcag agcaatcctc 120 ctgcctcagc ctcccaaggg ctaggatcac aggcgtgcgc caccacgccc agccttctta 180 tatttrgrrd dtttttttct tgctttwatt tgatttttct tttctttaca aatagaatta 240 scaccctt 248 777 92 DNA Murine misc_feature (1)...(92) n = A,T,C or G 777 ggaantccgg ctcgagcggc cgcttttttt ttttctctct ctctcagcct tctggttatc 60 gttacatgrg aacatcagac atactarrgg gg 92 778 633 DNA Murine misc_feature (1)...(633) n = A,T,C or G 778 ggaattcggg ggagaaagag agggagggag awwgwdagak akagagagak akakatcttd 60 ttctyctggc acaatattww ytktttnnnn dtdwgctnna aacttkttct ngtattttwt 120 kacawymrgg raattctttc ctctctaggc agattgccaa aaacaactag aagctaaatg 180 cctgtgcctt ctgcttctac gacacaccac tccgtcttgt tcagtttcaa ctagcgtcgc 240 tctaaaagga caaaaaactt cttgtttttc taaataaaac ataaatggcc cagaatttga 300 attgccgatc ttaaaatttt aagtgactga agattctatt aattctggca ataaaatcat 360 taaaaacaaa acaggttgca taagactttt aaacaattca ttcacaggca tgagaattta 420 aggtttcttt taaaatataa aatgctaaaa caataagtct aacaggagaa tatgaataat 480 acmatattct aagaaaaaaa cccacaaaga caaacatgac atttcattca tagctcattc 540 aaataaacca aggattaaac cttagtttta acctgttaat tttccttttt rytttagtat 600 gtctgatgtc dcatgtacgr tarccagaag gcc 633 779 443 DNA Murine misc_feature (1)...(443) n = A,T,C or G 779 ggaattcagg aatttvvbyw nvcwvdscwr myyycmykky skymtccgmc tcctcggggg 60 cmnctcctct cctagtgcca aagacatcav ganaatvctn gacagcgtgg gcatcgaagc 120 ggacgatgat cggctcaaca aggtcatcag tgagctgaat ggaaagnnca ttgdggntgt 180 catcgntcag ggtgttggca agctggccag tgtgcctgct ggtggggctg tggctgtttc 240 tgctgcccct ggctctgcag cacctgctgc tggttctgcc cccgctgcag cagaggagaa 300 gaaagatgag aagaaggagg agtccgagga gtcggatgac gacatgggat ttggcttgtt 360 tgattaaatt cctgctcccc tgcavataaa gtcbtttatg taaaaaaaaa aaaaaaaaag 420 cggccgtcga gccggtgaat tcc 443 780 225 DNA Murine misc_feature (1)...(225) n = A,T,C or G 780 ggaattcgtg agcagaaagc aatgaacata gatggtatac atactgtatg bstgtactvg 60 ctacavgrga catacamgca tgtccatcga agacttgaag agttctaaag cagcttacat 120 accagctcac ctgtcctgac ttcagttaaa tvtvgvscta tgntbccaac aacaggggaa 180 amcagactat acggtgattc tcctcagmgt gtcttgtttt tccta 225 781 428 DNA Murine misc_feature (1)...(428) n = A,T,C or G 781 ggaattcgtc ttcaacggct tctgtaaatc tcggtgaccc cacaaggcgt actgaaggag 60 attacttatc gtacagagag ttacattcaa tgggaagaac tccagtcatg tcaggatcac 120 agagacctct ttctgcacga gcgtacagca tcgatggccc aaatacatcc aggcctcaga 180 gtgcccgtcc ctctattaat grannvccag agagaactat gtcagntagt ganttcwaty 240 wctncacgga cntagtcctt caaaawgacc aaatacaagg ktcgggtctg aacatntctc 300 tgttagadcc yccakgdrwa agcaaggttc ctcatgactg gcgggancna gtactacgac 360 acattgaggc cnaaawgtta gaaaaggtaa ttbaactgag tttttcattc tcttwcttaa 420 tttwtttc 428 782 137 DNA Murine misc_feature (1)...(137) n = A,T,C or G 782 ggaattcaac thattkkact atbsvnvcav svancactdg tkctgctgct gctgctgctg 60 ctgctgctgc tgctgcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag 120 ctacavcggc kcacaca 137 783 612 DNA Murine misc_feature (1)...(612) n = A,T,C or G 783 ggaattnccc ggccctggca cagaggacta ggtgtgagag tgtgaggttc ccacccccac 60 ctttcctgcn gcbgctccct cccccsngac acagccaccc tccgtngctc accbbctggg 120 agcttgttgc ttcttgttca aggbgcgtaa ttbcgacact ctctagggcg cagggagccc 180 tgatttacat atttctcynn bgagtbcbtt ccnnctggta gggattnnnc tctcttbggt 240 tctgacacca nggnacaaga gtbcaracnn tnggaaaaaa cgactyccag gagctagcnt 300 tggcgntggg ccttggtacc cnattaggcc tttnyttctt ccaggttttt ccgctcnnyt 360 syktccckyc tttctttgct tactyctcac tcttctycct tctctctcct gtcttccyct 420 ctycctttcc tcgcctccat cctgckktct ckcctktctc cagtcnktct tagtrcctcc 480 atcctttccc tttactctct ctctnwctct myctctctct ctctcacaca cacacacaca 540 caccacacta gtgttctkkd aatwgtbaat hgtgatcccc cctgcgcctc cctctctcgg 600 cccyccccct cc 612 784 41 DNA Murine 784 ggaattccgg ctcgagcgga cgcttttttt ttttttttty t 41 785 217 DNA Murine 785 ggaattcctt aatttcatct agcctctggc ccaggaagag tgcacattta awgggactca 60 gagaaatgct gagacacatc aagagctgct gggcatccag gaagaatctg agtgcaaatt 120 tatcttttcc tgatgggtcg tcatcatcaa taattacatg ghgaycagtc aacaaaattk 180 taaaacccgg hmccaagtta caatcatgtg ttctgtc 217 786 290 DNA Murine misc_feature (1)...(290) n = A,T,C or G 786 ggaantcctt acctcaganc cctcatacag tgcactggta ccaacacctg ggaaggcagc 60 cctgcaaagg ggcccttctc ctccttgaac tatgtctgaa tctttgagta tctcattgtg 120 cccaadatgc atctgggaat tgtgcagaaa gacgttcaga aaagagggtg atamcagaga 180 hhycccgaat aaggggacca atgtatatgg bcyyycamtc acagtttcta tagrsscyby 240 bgtagacctt tbgttgggaa acgccctaca tctgggcata gcttcctttt 290 787 338 DNA Murine misc_feature (1)...(338) n = A,T,C or G 787 gnaattcgag tagattccca gtgctcacca tgagggaaac aatgttacta tamcdddncc 60 tantgnasgn nnnnnnnngc cngnggtaaa cngntagagg ntcctctgnt ncantgtnct 120 ttamacaymr ytwsrgtaga cagcaatgct ctttacctag cttagtgttc tgawkgsmaa 180 atattgtata ttgtgataat tatgtcctat ttatttgaga ttcttgttta aaatttaaaa 240 aacaaaaaaa caaatdaaaa tttttttgct atgccctaga tgtagggctt ttttttccaa 300 ccaaaggtct acaaaagttt ctatagaaac tgtgattg 338 788 213 DNA Murine misc_feature (1)...(213) n = A,T,C or G 788 ggaattcggc ttcaaaacac catgtaacat cttcaaaaga aaaavanntt twwwwmctca 60 aacgaatcag aaatgtaaat ccaggtccac gaacccacag ttaggcccag tggagtgaga 120 gggtccaggg aatagggggt ggggggaaga hagsnagcca aagtctcctg aggctcccct 180 cagatgtgta gaaaaattaa agataacycc aat 213 789 330 DNA Murine misc_feature (1)...(330) n = A,T,C or G 789 ggaattcccg gctcgagcgg cccctttttt ttggggggga gacgggggct cagggtgtga 60 acatgaggtg agacctggca tggcagggct gagtcgtgcc tgctgtcagc ccctctctgt 120 ccttcccgag gctgaggggg gcctcaagct cccttcccca gcagagcccc acccacccac 180 cccgccttca aagccccctt tggagagtta actgtccgtg tgaggcgctc actcaaccaa 240 taagccccaa ngaactcaaa gcaataatct tcgttactga gatgcgcggc tgttagtgtt 300 tngttgttgg kttttgnttt tttgggkttt 330 790 234 DNA Murine misc_feature (1)...(234) n = A,T,C or G 790 ggaattcntt atatcattkk vnknccatav ggbgtcatct atgtnbdaat ttwtynnnnc 60 acatatbssg ctcattacac ttatatcggg tcaacacagt ggcagatgnt catcaaatgt 120 ctttcattct aacacagtgg cagatgttat cagatgtctt tctttctaac agttacaagg 180 ccttgtctct acctcttccg cttccatgac ccagttttgg tgattctaat ttaa 234 791 417 DNA Murine misc_feature (1)...(417) n = A,T,C or G 791 ggaattcgta atccactaat atttatgggt gttatcacaa gtatamcaat nngntvgtca 60 actacaaadc aataaancag ttgcccaaat agcagcgtac gccctacgtt agcacagcca 120 ggtataaaga tccgtagcca caccaaactc tacaactgac tgttaagtgg cataacagta 180 aatagaggaa caacccatgt tcagggatta gtgagagggt ccagatgtta gaagctgcgc 240 ctcctcccca ctccttgtac tcactccatc acttaatgca actaaagcgt gttcttcttt 300 ccttttchct cctantctga caatgtantg ctgatattaa tttgaagvca atagccccaa 360 ctgccttgaa aacaaagaag tattatgagt tgtttgaaca catgggkatt aaaaaac 417 792 622 DNA Murine misc_feature (1)...(622) n = A,T,C or G 792 ggaattcgcg cgctgtcttc ccgctcgcgt cagggacctg cccgactcag cggccgccat 60 ggcatcagat gaaggcaagc ttttcgtggg aggactcagc ttcgacacca acgagcaggc 120 gctggagcag gtcttctcca agtatgggca gatctccgaa gtggtggtgg taaaggacag 180 gkagnaccca ngcgatcccg aggctttggg tttstcacct ttkaaaatat cgantgacgc 240 taaggancgc catgatggcn ntatngaant kggnaagtct ktggacgvgc nggcaratca 300 kagttgacca ggctngrcaa gtcttctgac maccggtycc gaggataccg gggtggctct 360 gctggaggcc ggggcttttt ccgtggggga cgaagccggg gccgagggtt ctccagagga 420 ggaggagacc ggggctatgg aggtggccgc tttgagtccc ggagtggggg ttatggaggc 480 tccagagact actatgccag ccggagtcag ggtggcagct atggttatcg gagctcggga 540 gggtcctaca gagacagcta tgacagttat gctacacaca acgggtaaag ccctccgcgt 600 ccagadycgt ccttccatgg ct 622 793 153 DNA Murine 793 caagttttgg ttgaattcct cggagcggcc tttttttttt tttttttttt tttttyyyyy 60 yyyytttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 120 tttttttttt tttttttttt tttaaaawwa aaa 153 794 603 DNA Murine misc_feature (1)...(603) n = A,T,C or G 794 ggaattccga ggcgccacag ccgctcccgg actcggagca gccgccgtct gccgcggagc 60 tggagtcttc ggccgaagaa tgcagctggg ccgggctttt ctccttccag gatctgcgag 120 ccgtgcatca gcagctgtgc tcggtaaact cccagctgga gccgtgtctg ccggtgttcc 180 ccgaagagcc gtcaggnatg tggacggtgc tgtttggggg cgnccccgag atgaccgagc 240 aggagatcga cgctctatgt taccaactcc aggtctacct gggccacggs ctggacacgt 300 gtggctggaa gatcctttct caggttcttt tcaccgagac ggatgatccg gaggagtatt 360 acgaaagcct cagcgagctg cggcagaagg gctatgaaga ggtgcttcag cgggccagga 420 ggcgcatcca ggagatgcaa agcttacagg gtggtacaga agctatagct cgattggmtc 480 agctggaatc tgactactat gayctgcaac ttcagttgta tgaagtacag tttgaaatct 540 tgaagtgtga agagttgtta ttaactgcac agctggagag catcaagaga cttatatcag 600 act 603 795 394 DNA Murine misc_feature (1)...(394) n = A,T,C or G 795 ggaattcgtt tttgtactgt taacattaac aatttttttt tttttvvbbs arrvgattcc 60 aggctttctt gacactatct ttactcttta tanactcagg aggtggtgct ccaagggcaa 120 agaatattac wrcwgactta gccaatttaa ctgctccagc tgggaataca ctctaaacag 180 aacccctaca atcagagtcc tatggctctc tctgaagagc aatgtaaatc aaacattagc 240 acatttctat tacctgctta aatgttcgaa gtctatccag tgtcctctgt ctctcttggc 300 taacccaggc actttttctt tcctcttcat catgcaattt gtctctcttt atttgtattg 360 tatgatgggc tctatattca tcttcactct gaaa 394 796 205 DNA Murine misc_feature (1)...(205) n = A,T,C or G 796 ggaattnccc ggctcgagcg gccacttttt ttttyttttt ttttwwtttt tttttttttt 60 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt scctttgggg 120 gttttthttb tttttaaahh tbktgsggdk tyacccgaam rggcmagcct tymcacccmg 180 gggggttwkt ccccaaaagg aggga 205 797 263 DNA Murine misc_feature (1)...(263) n = A,T,C or G 797 ggaattcgnt atttatatat avgcgatamt nbsggtttgk nacattagtt ttaaaaaagg 60 gaaagthttg ntytgtatan nnygttacct tttacagaat nhaagnattc aacattaagn 120 accatgtaac cgagacactt gatctgacac aggggcmgtc gggaaaccga tgactgcagt 180 aatcaccact gtacaaaaat gttagtgggt tttgtgcacg taaaatgcac acttccattt 240 cctgtcagtt tcttatttga vaa 263 798 422 DNA Murine misc_feature (1)...(422) n = A,T,C or G 798 ggaattcttt amvtgtttac catctactcg tgctgaatcc ttccaaggag attgctccag 60 cgtatatctc caggtcctcg ctttgctcct tttaccaaaa aatgcaaaac acaattccat 120 cgtgcatctt aagggctcca atcgtcaaaa ataggaaaaa aattctttgg aatagccaat 180 taagttgaat gaaaaaaaan tccnancacg aatgcggttt ggttggggca ggaaggaatc 240 cactcctatg tkcctggtaa tctgatcccg cnccatgaat cctggtaatt catcctccct 300 atcctatcca cattatgatt tgaatccaat gatcctactc cagtgattga caagtgtgca 360 ttgcctatgt ttgtttatvc bbkaattaca aaactatttt tttttaactt cctaagaatt 420 cc 422 799 159 DNA Murine misc_feature (1)...(159) n = A,T,C or G 799 ggtggaattc ggtcgagcgg ccgcnttttt tttttttttt ttthwwhhww mmmmmmmhhh 60 bbbbkkknnn kkkkkttttt twdggwacaa caagdtktaa dgnttttttt wctattnggg 120 kawaaamvgg tttwaangkt tttwwwaarn gggttttna 159 800 310 DNA Murine 800 scavmahtta cactatatac acyaaccatc awcamtcttt tacctatctc ttatccacta 60 ttaacttcct tcaattttyt ttttaattct tttttttgta ctactcatga tgttttyttc 120 cctcttcttt tttctaatyt tttttttccc aayayttata ctttaatata aamtctygtt 180 ttttcattcy acaaatdtty ttgctctcca wttytcattc ctttaaaatt csgrcwttta 240 attkcttbca aacbattttg ctttttgctc ckttttkatt gtccctcatt kaktctctkg 300 wcccatttgg 310 801 276 DNA Murine 801 ggaattcgtt tttttttttt tttttccctc tgtggtctaa gcttgtgggt cccagactta 60 gttgagataa agctggctgt tatctcaaag tcttcctcag ttccagcctg agaatcggca 120 tctaagtctt caaacatttc gttgctcgtt ttatgccctc atgagctctg accattgcat 180 gcgttcccat cccagctaca gaacttcagt ttataagcac acagtaacca ttcctcattg 240 catgatgccc tcaaataaaa ggtgaataca gtctat 276 802 170 DNA Murine misc_feature (1)...(170) n = A,T,C or G 802 ggaatyngga tbdrvargvc bttaggatga ctgtttgtat gggaagaagt tagagacaat 60 ggatttnadn acngatttnn bggataangc kttgtsattn nnnagtanca gggaagtaag 120 ycattttagc ctatncntta agvgtgaact acaggacagt aanctatata 170 803 260 DNA Murine misc_feature (1)...(260) n = A,T,C or G 803 ggaattcctc caatctacac ctatacttaa aaatcatgaa tctgactagc catgccattg 60 aaaaccactc agtactagag gatgaaccag ttttcaatgt tatcagccct ggaaaacacg 120 sccagctccc acccccagca cattctantt tngttttaac attttatarr ddnbgnbatt 180 dyhmktktgg ccatttttgt gggcaraaag ttaaatggct tccytttrag rctttggtsc 240 yggggcvadg ggagcacacc 260 804 203 DNA Murine misc_feature (1)...(203) n = A,T,C or G 804 ggaattcacc ggctcgaccg gccgcttttb tbtthhbyyn yynvtcatnc vdgnbyttyg 60 nnnnctgtgc ctagtavtnn ngvnnbccta aavctcacta gaatcctatt bbvgtatggy 120 amahbtyctt sgttctaaat ygggttkttt tgtcttggtt ttyytgtttt ycgttttgtt 180 ttgttttgtt gttaagagct aga 203 805 307 DNA Murine misc_feature (1)...(307) n = A,T,C or G 805 ggaattcgag gaatatcaac ttagtgctat twtcacatcg ttcagtcaaa cttagccaga 60 gttccaacnc cctacttaaa attcaactag aaagttacct accaagtact aattagcatt 120 ataahgtcag agcctgcagc tccaggcctt tcagttagtt gtttactaga aaggacagtc 180 ttaagccaga tacagtttmy cataaganah gttaaagmmt nccagtgaag caagttttty 240 ctttagccct agattccmgg cagnactatt gagcatagat aatyyccccc cctcaggcca 300 gcttttt 307 806 278 DNA Murine misc_feature (1)...(278) n = A,T,C or G 806 ggtgcaattc ccgdgcggcc vttttttttt tttttttttt tttttttttt ttttwwwwhn 60 nbbdrnnnnn nnnttttttt ttcactggnn atatntgntt tvtnccatca gacatgctgc 120 aatthtvgvc attyccmcag ggtwtagcag tkttaaamag tttgcatttg aacagctgac 180 atgaataaac aaacagtggg taggtgaata acctgcaaac gcgagttctg gattcacttt 240 tttgagctga gatgacagat tcagcaggaa ctctgtac 278 807 292 DNA Murine misc_feature (1)...(292) n = A,T,C or G 807 ggaattcctt ttmgmgcaag cctggggtcc agagcagatg tatttcgatt ctgaccctga 60 gtacgagggc ctgttcgata agmctcccct ggaggagggc cacactgcgc gtgcacctaa 120 gtctgcatcc tcggctggca ggaaatctgg tcggcgttcg agtgggaggg ctccggggac 180 ccgsgctggg ctgtcccgaa aggccssccs gtgcngtcca gansccmaag gaagaagagc 240 ctccagttgg aagagggctg ctaccttgac cacttgccyg gaacctbtgg ca 292 808 409 DNA Murine misc_feature (1)...(409) n = A,T,C or G 808 ggaattcatt ctttaaaaga ttattctgtc tgcaaagttt aaaaagtgtt gaaaaatatt 60 ctatacatct tgctcttaga acgtctccac tttgacagat caggtgaccc tcctcatcat 120 cctctatact cctggatctt ttccttcggt ggctcttctg gaacggcaag tgggcagcac 180 caagatcgtc cagccaatca atatatcaga gtgaattcag ggcagaatac gcaattcath 240 cagnsgggga gatattcagg canttcagrt aagcaccagg ccacgracag ttggcaggmg 300 caatttcaaa wtnmagtccc cagraattca caggscacag tttankgtta cagaagraaa 360 aacatggcaa ggmacagatt ttcagataag atacttaamg tggcaatag 409 809 238 DNA Murine 809 actagtacgg cgcagswggt ggaattcaga aatdactact gcgaaggata tgttccaaga 60 cattaccata gagacgttga aagcacttac cggatccatt gcagtaaatc ctcagtcagg 120 agcaggagar gcagccstaa gagaaagcgt aatagaccct gtgcaagtca tcagtcgcat 180 tcggttatga gtsgtttgtt ttawatttgg aatggattga ttgtaaagct ctgaaagg 238 810 391 DNA Murine misc_feature (1)...(391) n = A,T,C or G 810 ggaattcggg gsctaaagtc tgtctacatt acagatgggg ctggactgta ctctggtgtt 60 ctgggggatg tgggwkagat gagaagvrga aggcagcttg gcctgtccca cctattcaag 120 tgcctcccta aagcaggatn mgnaaagcta gghmcctagc caataagcag agcctttsgc 180 tgavggagaa agtagtcacc catgagaaat ccaaccacaa gtttgtgcca caggcaggtg 240 aatatgaggt ctccctgggg tgttggactc cctggactaa gaaaattaat acnaahhhhh 300 tgacactgca gtaccyctat ggagammvmc ntmtacagtt ttagtttggt agggtatcaa 360 gtcacntgta ccaatgtgac ctgttcaatc t 391 811 450 DNA Murine misc_feature (1)...(450) n = A,T,C or G 811 gcagctattv gbcatctcgm cctcmgctwt gcsacmgctt ggymcsnrst sgrkccacta 60 gtaacggncg ccagtgtggg ghattcccct tttgggyygt tctgaagctt gdtcttcawt 120 ctttaccctg gvctcaaatg nttgvctccc htcmathaac ctctttvctt tngttggtgc 180 ggngagttct cttttccctt tccctttctt atggcagccc ctaccttggc aactccactc 240 cactccacca ggtttgtctt cccagatagc agctggcaag ttctccaagg ctcagggtcc 300 taaggctcag tttgcagtgt gggcagtgtg taggaccagc ataatcttat tcttgttgcc 360 tccacaacac cacatcccct agtggttcgt gcgccccttg aagctctcca tagagctkgt 420 tgacactgcc actyagggcc tatgtccctt 450 

What is claimed is:
 1. An isolated polynucleotide comprising a nucleotide sequence containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 2. An isolated polynucleotide comprising a nucleotide sequence having a urogenital sinus-derived expressed sequence tag sequence at least 95% identical to a sequence comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 3. An isolated polynucleotide encoding a polypeptide wherein, except for at least one conservative amino acid substitution, said polypeptide has an amino acid sequence that is identical to a urogenital sinus-derived express sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 4. An isolated polynucleotide comprising a nucleotide sequence containing the urogenital sinus-derived expressed sequence tag comprising ug311.
 5. An isolated polynucleotide of claim 1 which is DNA.
 6. The isolated polynucleotide of claim 1 which is cDNA.
 7. The isolated polynucleotide of claim 1 which is genomic DNA.
 8. The isolated polynucleotide of claim 1 which is RNA.
 9. The isolated polynucleotide of claim 1 which further comprises a detectable label.
 10. A polynucleotide vector containing the polynucleotide of claim
 1. 11. A polynucleotide expression vector containing the polynucleotide of claim 1 in operative association with a nucleotide regulatory element that controls expression of the polynucleotide in a host cell.
 12. A cultured genetically engineered host cell containing the polynucleotide of claim
 1. 13. A cultured genetically engineered host cell containing the polynucleotide of claim 1 in operative association with a nucleotide regulatory element that controls expression of the polynucleotide in the host cell.
 14. The genetically engineered host cell of claim 13 which is prokaryotic.
 15. The genetically engineered host cell of claim 13 which is eukaryotic.
 16. A method of producing a polypeptide urogenital sinus-derived gene product, comprising the steps of: (a) growing the genetically engineered host cell of claim 14 in a culture; and (b) collecting the polypeptide gene product from the culture.
 17. A method of producing a polypeptide urogenital sinus-derived gene product, comprising the steps of: (a) growing the genetically engineered host cell of claim 15 in a culture; and (b) collecting the polypeptide gene product from the culture.
 18. An isolated polypeptide comprising the amino acid sequence encoded by the nucleotide sequence containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 19. A fusion protein comprising the polypeptide of claim 16 operatively associated with a heterologous polypeptide.
 20. An isolated polypeptide comprising a polypeptide having an amino acid sequence at least 95% identical to the amino acid sequence encoded by the nucleotide sequence containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 21. A fusion protein comprising the polypeptide of claim 18 or 20 operatively associated with a heterologous polypeptide.
 22. An isolated polypeptide comprising the amino acid sequence encoded by the nucleotide sequence containing the urogenital sinus-derived expressed sequence tag comprising ug311.
 23. A pharmaceutical composition comprising (a) An isolated polypeptide comprising the amino acid sequence encoded by the nucleotide sequence containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194; (b) pharmaceutically acceptable salts thereof; and a pharmaceutically acceptable carrier.
 24. A pharmaceutical composition comprising (a) An isolated polypeptide comprising a polypeptide having an amino acid sequence at least 95% identical to the amino acid sequence encoded by the nucleotide sequence containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194; (b) pharmaceutically acceptable salts thereof; and a pharmaceutically acceptable carrier.
 25. A method for diagnosing prostate disease, comprising assaying, in a patient sample, the expression of a polynucleotide containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 26. The method of claim 25 in which the expression of the polynucleotide is up-regulated.
 27. The method of claim 25 in which the expression of the polynucleotide is down-regulated.
 28. A method of monitoring the efficacy of a compound in clinical trials for the treatment of prostate disease, comprising assaying, in a patient sample, the expression of a polynucleotide containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 29. The method of claim 28 in which the expression of the polynucleotide is up-regulated.
 30. The method of claim 28 in which the expression of the polynucleotide is down-regulated.
 31. The method of claim 25 or 28 in which differential expression of the polynucleotide is assayed by: (a) obtaining a sample of cells from a patient; (b) assaying the expression of the polynucleotide in the sample of cells; and (c) comparing the expression level of the polynucleotide in the patient sample to the expression level of the polynucleotide in a control sample of cells, in which a difference in the expression level of the polynucleotide in the patient sample and the control indicates differential expression of the polynucleotide.
 32. A method for diagnosing prostate disease, comprising determining, in a patient sample, the presence of a mutation in a gene containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 33. The method of claim 32 in which the mutation of the gene is assayed by: (a) obtaining a sample of cells from the patient; (b) analyzing the structure of the gene in genomic DNA obtained from the sample of cells; and (c) comparing the structure of the gene in the patient sample to the structure of the gene in a control sample of cells, in which a difference in the structure of the gene in the patient sample and the control indicates a mutation in the gene in the patient.
 34. A method for identifying a substance for treating prostate disease comprising assaying the ability of a test substance to modulate the expression of a gene containing the urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194.
 35. The method of claim 34 in which the prostate disease is prostatitis, benign or malignant growth of the prostate gland.
 36. The method of claim 34 in which the modulation of the expression of said gene is assayed by: (a) exposing a sample of cells to a test substance; (b) assaying the expression of said gene in the sample of cells; and (c) comparing the expression level of the gene in the sample exposed to the substance to the expression level of the gene in a control sample of cells, in which a difference between the expression level of the gene in the sample exposed to the substance and the control indicates the modulation of expression of the gene.
 37. The method of claim 34 in which the gene is down-regulated by the test substance.
 38. The method of claim 36 in which the substance is an oligonucleotide complementary to the 5′ region of the gene and blocks transcription via triple helix formation.
 39. The method of claim 38 in which the substance is an antisense or ribozyme molecule that blocks translation of the gene.
 40. The method of claim 34 in which the gene is up-regulated by the test substance.
 41. The method of claim 34 in which the substance is a small organic or inorganic molecule that modulates the activity of the protein product by binding to the protein product.
 42. The method of claim 34 in which the substance is an antibody that modulates the activity of the protein product by binding to the protein product.
 43. An assay for identifying a substance that binds to the protein encoded by a gene comprising: (a) contacting a protein or peptide containing an amino acid sequence corresponding to the binding site of the protein encoded by a gene containing a urogenital sinus-derived expressed sequence tag comprising ug092, ug093, ug096, ug101, ug102, ug106, ug120, ug254, ug291, ug307, ug308, ug311, ug317, ug320, ug334, ug335, ug353, ug354, ug357, ug440, ug441, ug482, ug484, ug485, ug491, ug493, ug494, ug503, ug505, ug506, ugs148, ugs186, and ugs194, with a test substance, under conditions and for a time sufficient to permit binding and formation of a complex between the protein or peptide and the test substance, and (b) detecting the formation of a complex, in which the ability of the test substance to bind to the protein is indicated by the presence of the test substance in the complex. 